Evaluation of a chromogenic medium supplemented with glucose for detecting Enterobacter sakazakii.

Abstract:

:A commercial chromogenic agar medium (DFI) was supplemented with glucose (mDFI) to enhance the specificity of Enterobacter sakazakii (E. sakazakii) detection. Escherichia vulneris (E. vulneris), a putative false-positive strain on the DFI medium, produces alpha-glucosidase. The enzyme alpha- glucosidase hydrolyzes a substrate, 5-bromo-4-chloro-3- indolyl-alpha,D-glucopyranoside (XalphaGlc), producing green colonies. E. sakazakii strains produced green colonies on both DFI and mDFI agar, whereas E. vulneris produced green colonies on DFI agar but small white colonies on mDFI agar. E. sakazakii and E. vulneris were also readily differentiated by colony color when the mixed culture of the two strains was plated on mDFI agar and incubated for 24 h at 37 degrees C. The results indicate that the selectivity of the commercial chromogenic agar medium could be improved by a simple supplementation with glucose.

journal_name

J Microbiol Biotechnol

authors

Song KY,Hyeon JY,Shin HC,Park CK,Choi IS,Seo KH

subject

Has Abstract

pub_date

2008-03-01 00:00:00

pages

579-84

issue

3

eissn

1017-7825

issn

1738-8872

pii

7167

journal_volume

18

pub_type

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