Abstract：
:The guinea-pig (gp) CD4 protein was identified using the rat monoclonal antibody (mAb) H155 derived from an interspecies hybrid. The hybrid was obtained after immunization of rats with purified guinea-pig T lymphocytes and fusion to a mouse myeloma line. The mAb H155 reacted with a subpopulation (60-70%) of nylon-wool-purified guinea-pig T cells. The majority of thymocytes also expressed the H155 antigen. Immunohistological staining showed that predominantly the cortical thymocytes were H155-positive, whereas a part of the medullary cells did not express the antigen. After cell-surface radioiodination of T cell lines, the mAb H155 immunoprecipitated a molecule of about 55,000 MW both under reducing and non-reducing conditions. The antigen recognized by mAb H155 on guinea-pig T cells resembles the human or mouse CD4 antigen. Depletion experiments in combination with functional studies further supported this assumption. In proliferation assays mAb H155 inhibited T-cell responses in vitro. Both the antigen- and alloantigen-induced T-cell activation were impaired in the continuous presence of mAb H155. In addition, mAb H155 inhibited both the mitogen-induced T-cell activation and T-cell proliferation induced by mitogenic mAb and phorbol myristate acetate (PMA). Binding of the mAb H155 to the CD4 molecule might therefore transduce a negative signal on T-cell activation.
journal_name
Immunologyjournal_title
Immunologyauthors
Schäfer H,Burger Rsubject
Has Abstractpub_date
1991-02-01 00:00:00pages
261-8issue
2eissn
0019-2805issn
1365-2567journal_volume
72pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:The epigenetic regulation of transcription factor genes is critical for T-cell lineage specification. A specific methylation pattern within a conserved region of the lineage specifying transcription factor gene FOXP3, the Treg-specific demethylated region (TSDR), is restricted to regulatory T (Treg) cells and is requi...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12261
更新日期：2014-07-01 00:00:00
abstract：:Mouse models of multiple sclerosis (MS) have shown the importance of interleukin-10 (IL-10) -producing regulatory B (Breg) cells in dampening disease activity and inhibiting disease initiation and progression. In MS and other autoimmune diseases decreased frequency and functionality of Breg cells correlate with diseas...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.13024
更新日期：2019-03-01 00:00:00
abstract：:Lymphocyte migration into inflammatory sites involves a change from a spherical, non-motile phenotype to an irregular, constantly shape-changing, motile phenotype. We have previously shown that lymphocytes are maintained in the non-motile state by the constitutive activity of protein kinase C (PKC). In this paper we h...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1996.486567.x
更新日期：1996-03-01 00:00:00
abstract：:Culture dishes precoated with thin layers of acid soluble rat tail collagen simplify conditions necessary to obtain in vitro high IgG anti-DNP responses from primed and boosted mice. In contrast to Mishell-Dutton type cultures the use of collagen precludes the need to continually rock cultures and to add nutrients. Re...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
abstract：:Anti-Gal is the most abundant natural antibody in humans, constituting ~ 1% of immunoglobulins. Anti-Gal is naturally produced also in apes and Old World monkeys. The ligand of anti-Gal is a carbohydrate antigen called the 'α-gal epitope' with the structure Galα1-3Galβ1-4GlcNAc-R. The α-gal epitope is present as a maj...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/imm.12110
更新日期：2013-09-01 00:00:00
abstract：:The results presented in this paper demonstrate that a mouse IgM monoclonal antibody (T-80) recognizes an antigen on cells of the T-lymphocyte lineage of sheep. However, this antibody does not identify all T cells, as 10-20% of thymocytes and some peripheral-blood T cells are negative. T-80- thymocytes reside in the m...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:The non-specific normal immunosuppressive protein (Nip) has been described in our laboratory and its biological activity was extensively studied. In the present study, further purification analyses of Nip were conducted. Fractionation of Nip by Ultrogel AcA 34 column resulted in peak (I) that displayed Nip activity in...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：:Free malarial merozoites isolated from in vitro cultures of P. knowlesi and emulsified with Freund's complete (FCA) or incomplete (FIA) adjuvant were used to vaccinate twelve Rhesus monkeys against the uniformly lethal infection caused by P. knowlesi. Initial challenge of six monkeys with the same parasite variant as ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：:Purified cleavage products of the guinea-pig complement component C3, namely C3b and C3a, interact with guinea-pig and mouse macrophages in culture to induce a dose- and time dependent release of lysosmal enzymes into the medium. In the case of C3b the selectivity of the release of hydrolases, which occurs without cel...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:Myelin oligodendrocyte glycoprotein (MOG), a minor protein of the central nervous system myelin, is recognized as a potential target in multiple sclerosis and neuromyelitis optica. The extracellular domain of MOG is commonly used in a wide range of mouse strains and other animals to induce experimental autoimmune ence...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12155
更新日期：2013-12-01 00:00:00
abstract：:Chloroquine treatment of antigen-presenting cells (APC) was explored as a tool to investigate the processing pathway for major histocompatibility complex (MHC) class II-restricted presentation of the endogenous secreted hen egg lysozyme (HEL) and transmembrane measles virus haemagglutinin (HA). A 72-hr pretreatment of...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:Recent studies involving T cells that express gamma delta T-cell receptor (gamma delta TcR) have raised the possibility that Qa-1/Tla region class I major histocompatibility complex (MHC)-like molecules are antigen-presenting molecules for gamma delta TcR. In this report, cytotoxic T lymphocyte (CTL) clones specific f...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:The role of the mouse homocytotropic antibodies in passive cutaneous anaphylaxis reaction was investigated. One class of antibody was heat stable, detected at 2 h but not at 48 h after passive transfer, and belonged to a sublcass of mouse IgG. The other was heat labile, detected at 2 h and 48 h after passive transfer,...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1977-04-01 00:00:00
abstract：:The effect of disodium cromoglycate (DSCG) upon human immunoglobulin (Ig) isotypes and IgG subclasses production by purified B cells was studied. DSCG enhanced IgM, IgG1, IgG2, IgG3, IgG4 and IgA production in a dose-dependent fashion, while DSCG failed to induce IgE production at any concentrations tested by purified...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:The fate of allogeneic lymphocytes (AO or DA) transferred to non-immune PVG recipients was studied in the light of previous evidence (Heslop & McNeilage, 1983; Rolstad & Ford, 1983) that allogeneic lymphocytes can be rapidly destroyed in certain strain combinations of rats and mice by a mechanism that is distinct from...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Isolated normal human peripheral neutrophils became cytotoxic to chicken red blood cells (CRBC) in the presence of phytohaemagglutinin (PHA) and concanavalin A (Con A), a phenomenon which we have termed lectin-dependent neutrophilmediated cytotoxicity (LDNMC). Substantial cytotoxicity could be demonstrated by 1 h of i...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:An investigation was made of the properties of cytotoxic T cells induced by Con A and exposure to LCM virus-infected cells. As a basis for such studies, the optimal conditions for in vitro Con A stimulation of in vivo LCM virus-primed C3H mouse splenocytes were determined. The most potent cytotoxicity was obtained whe...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:Activation of lymph nodes by the T-cell mitogens PHA and Con A is correlated with a depletion of lymphatic mast cells. This result, and our earlier reports on the depletion of mast cells in lymph nodes stimulated by allogeneic and tumour cells, as well as on the elevation of mast cell number in thymus-less 'nude' mice...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:Different kinds of IgM antibodies were tested for their activity in antibody-dependent cellular cytotoxicity (ADCC): firstly an anti-benzylpenicilloyl (BPO) IgM antibody from immune rabbit serum purified by affinity, ion exchange, and molecular-sieving chromatography, secondly two monoclonal rat anti-BPO IgM antibodie...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
abstract：:Bile acids (BAs) play important roles not only in lipid metabolism, but also in signal transduction. TGR5, a transmembrane receptor of BAs, is an immunomodulative factor, but its detailed mechanism remains unclear. Here, we aimed to delineate how BAs operate in immunological responses via the TGR5 pathway in human mon...
journal_title：Immunology
pub_type： 临床试验,杂志文章
doi：10.1111/imm.12045
更新日期：2013-05-01 00:00:00
abstract：:We have previously shown that chlorpromazine (CPZ) inhibits tumour necrosis factor (TNF) production and protects against endotoxic shock in mice. In this paper we investigated the effect of pretreatment with CPZ, 4 mg/kg i.p. 30 min before, compared with dexamethasone (DEX; 3 mg/kg) on the induction of other endotoxin...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Age-related macular maculopathy (ARM) and age-related macular degeneration (AMD) are the leading causes of blindness in the Western world. Despite the magnitude of this clinical problem, very little is known about the pathogenesis of the disease. In this study, we analysed the sera (using indirect immunohistochemistry...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2005.02173.x
更新日期：2005-07-01 00:00:00
abstract：:Hepatitis C virus (HCV) infection is a global health problem characterized by a high rate of chronic infection, which may in part be due to a defect in myeloid dendritic cells (mDCs). This defect appears to be remedied by treatment with interferon-α (IFN-α) -based antiviral therapies; however, the molecular mechanisms...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12350
更新日期：2014-12-01 00:00:00
abstract：:alpha 1-Acid glycoprotein (AG), a serum component elevated during acute inflammation, has been implicated in the suppression of various immunological responses. Pretreatment of lymphoid cells with AG at a concentration commonly found in patients with acute inflammation results in the inhibition of mitogen induced lymp...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is an autoimmune disease that involves dysregulation of B and T cells. A tolerogenic peptide, designated hCDR1, ameliorates disease manifestations in SLE-afflicted mice. In the present study, the effect of treatment with hCDR1 on the CD74/macrophage migration inhibitory factor (MIF) ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2010.03342.x
更新日期：2011-01-01 00:00:00
abstract：:One of the hallmarks of systemic autoimmune diseases, such as systemic lupus erythematosus (SLE), is the immune response to nuclear autoantigens. Several studies have proposed that dendritic cells may acquire the nuclear autoantigens from the apoptotic cells to initiate the systemic autoimmune responses. To examine th...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2002.01438.x
更新日期：2002-07-01 00:00:00
abstract：:Lymphocytes are heterogeneous with respect to their life-span. Typical B cells, bearing on their membranes immunoglobulin receptors, easily detectable by immunofluorescence, belong mainly to the long-lived population: this can be observed using combined autoradiography and immunofluorescence. However, when primed mice...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1975-05-01 00:00:00
abstract：:The effect of long chain and medium chain fatty acid absorption on transport and mitogen-induced blast transformation of lymphocytes in intestinal lymphatics was investigated. Intestinal lymph was collected from mesenteric lymph duct cannulated rats maintained in Bollman's cage. Following the intraduodenal administrat...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Free fatty acids (FFA) were tested for their effects on in vitro thymus-dependent (TD) and thymus-independent (TI) immune responses. Murine T cells proliferating in one-way mixed leucocyte reactions (MLR) were extremely sensitive to inhibition by exogenous stearic acid (18:0) but were only moderately affected by oleic...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:Degradation of the extracellular matrix plays an important role in rheumatoid articular destruction. Rheumatoid synovial fibroblasts secrete a large amount of matrix-degrading metalloproteinases (MMPs), which initiate tissue damage by proteolytic degradation of collagens and proteoglycans. Cytokines, such as interleuk...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1996.d01-789.x
更新日期：1996-12-01 00:00:00
