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Protein kinase C isotype expression and regulation of lymphoid cell motility.

Abstract：

:Lymphocyte migration into inflammatory sites involves a change from a spherical, non-motile phenotype to an irregular, constantly shape-changing, motile phenotype. We have previously shown that lymphocytes are maintained in the non-motile state by the constitutive activity of protein kinase C (PKC). In this paper we have attempted to identify the PKC isotype which regulates these morphological changes by three different approaches. (a) Motile and non-motile T-cell lines were compared for expression of the alpha, beta I, beta II, gamma, delta, epsilon, eta, zeta and theta isotypes by Western blotting. There was no obvious correlation of isotype expression with motility. (b) Two different PKC inhibitors, one specific for classical isotypes, Go6976 and the other GF109203X, which inhibits both classical and non-classical isotypes were compared for induction of motility in non-motile lymphocytes. Only GF109203X induced motility implying that a non-classical isotype is involved. (c) Non-motile lymphocytes were chronically treated with the PKC activator bryostatin and the time courses of induction of motility and downregulation of PKC isotypes were compared. Induction of motility correlated better with downregulation of epsilon, eta and theta than with alpha or beta. It is concluded that the data fit best with the involvement of a non-classical PKC isotype in regulating lymphocyte motility although no association with a particular isotype was found.

journal_name

Immunology

journal_title

Immunology

authors

Thorp KM,Verschueren H,De Baetselier P,Southern C,Matthews N

doi

10.1046/j.1365-2567.1996.486567.x

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

434-8

issue

3

eissn

0019-2805

issn

1365-2567

journal_volume

87

pub_type

杂志文章

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