Abstract:
:The mammalian GADD45 (growth arrest and DNA-damage inducible) gene family is composed of three highly homologous small, acidic, nuclear proteins: GADD45alpha, GADD45beta, and GADD45gamma. GADD45 proteins are involved in important processes such as regulation of DNA repair, cell cycle control, and apoptosis. Annotation of the Drosophila melanogaster genome revealed that it contains a single GADD45-like protein (CG11086; D-GADD45). We found that, as its mammalian homologs, D-GADD45 is a nuclear protein; however, D-GADD45 expression is not elevated following exposure to genotoxic and nongenotoxic agents in Schneider cells and in adult flies. We showed that the D-GADD45 transcript increased following immune response activation, consistent with previous microarray findings. Since upregulation of GADD45 proteins has been characterized as an important cellular response to genotoxic and nongenotoxic agents, we aimed to characterize the effect of D-GADD45 overexpression on D. melanogaster development. Overexpression of D-GADD45 in various tissues led to different phenotypic responses. Specifically, in the somatic follicle cells overexpression caused apoptosis, while overexpression in the germline affected the dorsal-ventral polarity of the eggshell and disrupted the localization of anterior-posterior polarity determinants. In this article we focused on the role of D-GADD45 overexpression in the germline and found that D-GADD45 caused dorsalization of the eggshell. Since mammalian GADD45 proteins are activators of the c-Jun N-terminal kinase (JNK)/p38 mitogen-activated protein kinase (MAPK) signaling pathways, we tested for a genetic interaction in D. melanogaster. We found that eggshell polarity defects caused by D-GADD45 overexpression were dominantly suppressed by mutations in the JNK pathway, suggesting that the JNK pathway has a novel, D-GADD45-mediated, function in the Drosophila germline.
journal_name
Geneticsjournal_title
Geneticsauthors
Peretz G,Bakhrat A,Abdu Udoi
10.1534/genetics.107.079517subject
Has Abstractpub_date
2007-11-01 00:00:00pages
1691-702issue
3eissn
0016-6731issn
1943-2631pii
177/3/1691journal_volume
177pub_type
杂志文章相关文献
GENETICS文献大全abstract::Cyclin E together with its kinase partner Cdk2 is a critical regulator of entry into S phase. To identify novel genes that regulate the G1- to S-phase transition within a whole animal we made use of a hypomorphic cyclin E mutation, DmcycEJP, which results in a rough eye phenotype. We screened the X and third chromosom...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.026617
更新日期:2004-09-01 00:00:00
abstract::The Polymerase Associated Factor 1 complex (Paf1C) is a multifunctional regulator of eukaryotic gene expression important for the coordination of transcription with chromatin modification and post-transcriptional processes. In this study, we investigated the extent to which the functions of Paf1C combine to regulate t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302262
更新日期:2019-07-01 00:00:00
abstract::We have tested the hypothesis that genetic differences among conspecific populations may result in diverse responses to selection, using natural populations of Drosophila melanogaster. Selection for ethanol tolerance in a tube measuring knockdown resistance was imposed on five West Coast populations. In 24 generations...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-09-01 00:00:00
abstract::Low-coverage next-generation sequencing methodologies are routinely employed to genotype large populations. Missing data in these populations manifest both as missing markers and markers with incomplete allele recovery. False homozygous calls at heterozygous sites resulting from incomplete allele recovery confound man...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.182071
更新日期:2016-02-01 00:00:00
abstract::We have characterized a Saccharomyces cerevisiae mutant strain that is hypersensitive to cyclosporin A (CsA) and FK506, immunosuppressants that inhibit calcineurin, a serine-threonine-specific phosphatase (PP2B). A single nuclear mutation, designated cev1 for calcineurin essential for viability, is responsible for the...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::Evolutionary forces shape patterns of genetic diversity within populations and contribute to phenotypic variation. In particular, recurrent positive selection has attracted significant interest in both theoretical and empirical studies. However, most existing theoretical models of recurrent positive selection cannot e...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.156935
更新日期:2014-05-01 00:00:00
abstract::Positive selection on a new mutant allele can increase the frequencies of closely linked alleles (through hitchhiking), as well as create linkage disequilibrium between them. Because this disequilibrium is induced by the selected allele, one may be able to identify loci under selection by measuring the influence of a ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::A genetic model with additive-dominance effects and genotype x environment interactions is presented for quantitative traits with time-dependent measures. The genetic model for phenotypic means at time t conditional on phenotypic means measured at previous time (t-1) is defined. Statistical methods are proposed for an...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::Paramecium has long been a model eukaryote. The sequence of the Paramecium tetraurelia genome reveals a history of three successive whole-genome duplications (WGDs), and the sequences of P. biaurelia and P. sexaurelia suggest that these WGDs are shared by all members of the aurelia species complex. Here, we present th...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.163287
更新日期:2014-08-01 00:00:00
abstract::The reproductive success of individual fire ant queens (Solenopsis invicta) previously has been shown to be strongly influenced by their genotype at a single enzyme-encoding gene, designated Pgm-3. This paper presents evidence that a second, tightly linked gene, designated Gp-9, is under similarly strong selection in ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::Hybrid male sterility (HMS) contributes to reproductive isolation commonly observed among house mouse (Mus musculus) subspecies, both in the wild and in laboratory crosses. Incompatibilities involving specific Prdm9 alleles and certain Chromosome (Chr) X genotypes are known determinants of fertility and HMS, and previ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303474
更新日期:2020-10-01 00:00:00
abstract::A likelihood ratio test is presented for comparing rates of evolutionary change in the paths of descent leading to two species. The test is compared to previous relative rate tests based on variances of estimated numbers of base substitutions. The likelihood approach allows for different transversion and transition ra...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
abstract::Evolutionary rescue describes a situation where adaptive evolution prevents the extinction of a population facing a stressing environment. Models of evolutionary rescue could in principle be used to predict the level of stress beyond which extinction becomes likely for species of conservation concern, or, conversely, ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.300908
更新日期:2018-05-01 00:00:00
abstract::Over one-third of human genome sequence is a product of non-LTR retrotransposition. The retrotransposon that currently drives this process in humans is the highly abundant LINE-1 (L1) element. Despite the ubiquitous nature of L1's in mammals, we still lack a complete mechanistic understanding of the L1 replication cyc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.096636
更新日期:2009-01-01 00:00:00
abstract::Acrylamide gel electrophoresis was performed on the enzyme xanthine dehydrogenase in sixty isochromosomal lines of Drosophila persimilis from three geographic populations. Sequential electrophoretic analysis using varied gel concentrations and buffers revealed twenty-three alleles in this species where only five had b...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::Epistasis contributes significantly to intrapopulation variation in floral morphology, development time, and male fitness components of Mimulus guttatus. This is demonstrated with a replicated line-cross experiment involving slightly over 7000 plants. The line-cross methodology is based on estimates for means. It thus...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.041525
更新日期:2005-12-01 00:00:00
abstract::The pathogenic life cycle of the rice blast fungus Magnaporthe oryzae involves a series of morphogenetic changes, essential for its ability to cause disease. The smo mutation was identified > 25 years ago, and affects the shape and development of diverse cell types in M. oryzae, including conidia, appressoria, and asc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301490
更新日期:2019-01-01 00:00:00
abstract::Smg1 is a key component of nonsense-mediated decay (NMD) in Caenorhabditis elegans and mammals. Here we report that two nonsense alleles of the ortholog of Smg1 do not affect NMD in Drosophila melanogaster. ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.045674
更新日期:2005-09-01 00:00:00
abstract::Translocation induced in the immature oocyte, in meiotic prophase, affects division I orientation and segregation, the usual result being that the two halves of translocations are directed to opposite poles. Since interchange is usually (if not exclusively) between chromatids, this is to be expected from the creation ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-09-01 00:00:00
abstract::Despite its importance to agriculture, the genetic basis of heterosis is still not well understood. The main competing hypotheses include dominance, overdominance, and epistasis. NC design III is an experimental design that has been used for estimating the average degree of dominance of quantitative trait loci (QTL) a...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.082867
更新日期:2008-11-01 00:00:00
abstract::Inbreeding depression, which refers to reduced fitness among offspring of related parents, has traditionally been studied using pedigrees. In practice, pedigree information is difficult to obtain, potentially unreliable, and rarely assessed for inbreeding arising from common ancestors who lived more than a few generat...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.130922
更新日期:2011-09-01 00:00:00
abstract::Two sets of overlapping experiments were conducted to examine recombination and spontaneous mutation events within clusters of resistance genes in lettuce. Multiple generations were screened for recombinants using PCR-based markers flanking Dm3. The Dm3 region is not highly recombinagenic, exhibiting a recombination f...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract::The K1 killer plasmid, [KIL-k1], of Saccharomyces cerevisiae is a 1.25 x 10(6) dalton linear double-stranded RNA plasmid coding for a protein toxin and immunity to that toxin. The [KIL-sd1] plasmid is a replication-defective mutant of [KIL-k1] that depends on one of the recessive chromosomal superkiller (ski-) mutatio...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::A model of the evolution of a transposable element family in a Mendelian host population is proposed that incorporates heritable phenotypic mutations in the elements. The temporal behavior of the numbers of mutant and wild-type elements is studied, and the expected extinction time of the transposable element family is...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::Seed component structures were grouped into maternal and offspring (embryo and endosperm) tissues to characterize a dormancy quantitative trait locus (QTL) for tissue-specific function using a marker-assisted genetic approach. The approach was devised to test if genotypic/allelic frequencies of a marker tightly linked...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.092007
更新日期:2008-08-01 00:00:00
abstract::The inbred mouse strain, C57BL/6J, was derived from mice of the Mus musculus complex. C57BL/6J can be crossed in the laboratory with a closely related mouse species, M. spretus to produce fertile offspring; however there has been no previous evidence of gene flow between M. spretus and M. musculus in nature. Analysis ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract::Four crosses were made between inbred Cannabis sativa plants with pure cannabidiol (CBD) and pure Delta-9-tetrahydrocannabinol (THC) chemotypes. All the plants belonging to the F(1)'s were analyzed by gas chromatography for cannabinoid composition and constantly found to have a mixed CBD-THC chemotype. Ten individual ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Post-transcriptional RNA processing is an important regulatory control mechanism for determining the phenotype of eukaryotic cells. The processing of a transcribed RNA species into alternative splice isoforms yields products that can perform different functions. Each type of cell in a multi-cellular organism is presum...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.066183
更新日期:2007-03-01 00:00:00
abstract::Actin is a major component of the cytoskeleton and one of the most abundant proteins found in eukaryotic cells. Comparative sequence analysis shows that this essential gene has been highly conserved throughout eukaryotic evolution making it useful for phylogenetic analysis. Complete cDNA clones for the actin-encoding ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract::The satellite DNA's from the embryos of five species of Drosophila (D. melanogaster, D. simulans, D. nasuta, D. virilis and D. hydei) have been analyzed for the presence of closed circular duplex DNA molecules, as determined by CsCl-EBr gradients. Circular DNA molecules were found in every species but D. melanogaster....
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1972-11-01 00:00:00