Abstract：
AIMS:The aims of the study were to compare bacterial recovery following storage of sputum samples at 20 degrees C room temperature and 4 degrees C (refrigerated) for 24 h, and to determine the effect of postage on viable bacterial numbers. METHODS:A total of 38 individual sputum samples from clinically stable patients with bronchiectasis were split into three equal aliquots and quantitative bacterial culture was performed (i) immediately, (ii) following storage at 4 degrees C for 24 h or (iii) following storage at 20 degrees C for 24 h. A further 42 sputum samples were split into two equal aliquots and quantitative bacterial culture was performed either immediately or following postage back to the laboratory by first-class mail from an outside location. RESULTS:The predominant organism could still be recovered following storage at 4 degrees C and 20 degrees C, but viable numbers were significantly reduced following storage at 4 degrees C (p<0.004) by at least an order of magnitude (10-fold) in 24% of samples stored at 4 degrees C compared with only 8% stored at 20 degrees C. Posting samples back to the laboratory did not affect the recovery of bacterial species and there was no difference in viable numbers isolated. CONCLUSIONS:The results suggest that storage at room temperature is preferable to refrigeration as it retains the species isolated and the viable number. The data also confirm that sputum samples can be posted to the laboratory from patients in the community without affecting qualitative or quantitative results.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Pye A,Hill SL,Bharadwa P,Stockley RAdoi
10.1136/jcp.2007.051490subject
Has Abstractpub_date
2008-03-01 00:00:00pages
352-4issue
3eissn
0021-9746issn
1472-4146pii
jcp.2007.051490journal_volume
61pub_type
杂志文章abstract：AIMS:This cross-sectional study investigated the association between eight herpesviruses and the bacterial community profiles from the oral cavity of children with and without leukaemia. METHODS:Sixty participants (aged 3-13), divided into the leukaemia group (LG) and healthy group (HG), were evaluated. Collection of ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2014-202668
更新日期：2015-03-01 00:00:00
abstract：:The mean areas of lymphoid follicles have been determined in 20 specimens of malignant follicular (centrocytic-centroblastic) lymphoma, 15 specimens of reactive follicular hyperplasia and 10 palatine tonsils. In all of the benign specimens and whenever possible for those showing malignancy, the areas of both the whole...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.36.9.1055
更新日期：1983-09-01 00:00:00
abstract：:Strokes are one of the most common causes of mortality and long term severe disability. There is evidence that lipoprotein (a) (Lp(a)) is a predictor of many forms of vascular disease, including premature coronary artery disease. Several studies have also evaluated the association between Lp(a) and ischaemic (thrombot...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.53.7.487
更新日期：2000-07-01 00:00:00
abstract：AIM:To assess the clinical value of malignancy grading systems compared with nuclear DNA content, protein p53, and angiogenesis for predicting recurrence of stage I (UICC, 1987) tongue carcinomas. METHODS:Histopathological malignancy grading according to Jakobsson and tumour front grading according to Bryne et al were...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.52.1.35
更新日期：1999-01-01 00:00:00
abstract：:A patient with megaloblastic anaemia due to nutritional folate deficiency is described. Partial villous atrophy and malabsorption of xylose showed progressive improvement to normal with folic acid therapy. ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.24.2.131
更新日期：1971-03-01 00:00:00
abstract：:It was shown that, on average, the titres of the sera obtained by both the anti-complement immunofluorescence (ACIF) and indirect immunofluorescence for viral late antigens (IFA-LA) procedures were approximately eight times higher than those obtained by complement fixation. There was no significant difference between ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.31.9.827
更新日期：1978-09-01 00:00:00
abstract：:Although Neisseria species other than N gonorrhoeae and N meningitidis normally comprise part of the commensal bacterial flora of the oropharynx, they may occasionally act as opportunistic pathogens. Infections in which these organisms have been implicated include cases of endocarditis, meningitis, septicaemia, otitis...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.36.2.213
更新日期：1983-02-01 00:00:00
abstract：:A new rapid automated method for the identification and classification of microorganisms is described. It is based on the incorporation of 35S-methionine into cellular proteins and subsequent separation of the radiolabelled proteins by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The protein ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.40.9.1070
更新日期：1987-09-01 00:00:00
abstract：:Methods are described for the estimation of plasma and urine alpha-amino nitrogen by the colorimetric ninhydrin reaction; in the method for urine, both ammonia and urea are separated by a rapid ion-exchange resin procedure. Two methods of estimation are given; one has the advantage of great simplicity, but involves th...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.17.2.150
更新日期：1964-03-01 00:00:00
abstract：:SF3B1 is the largest subunit of the Spliceosome Factor 3b (SF3B) complex and part of the U2 small nuclear ribosomal protein. It functions as an important part of spliceosomal assembly, converting precursor messenger RNA (mRNA) to mRNA ready for ribosomal translation. Mutations of SF3B1 are commonly seen in myelodyspla...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jclinpath-2019-205895
更新日期：2019-11-01 00:00:00
abstract：:The proportion of fimbriate strains amongst Escherichia coli freshly isolated from infected urines did not differ significantly from the proportion amongst commensal Esch. coli isolated from the bowel and from non-infected urines. It is concluded that fimbriae play no major role in the pathogenesis of Esch. coli urina...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.20.1.84
更新日期：1967-01-01 00:00:00
abstract：:Four cases of human infection with Achromobacter anitratus, one of which was fatal, are described. They all occurred at one hospital within a period of two months. The bacteriology, antibiotic sensitivity, and pathogenicity of the organisms are discussed. One of the strains was found to differ from the other three. ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.14.6.670
更新日期：1961-11-01 00:00:00
abstract：:The presence of serum agglutinins and precipitins to Candida albicans in men and women who had impaired bladder function following spinal injury is compared with that in other patients in hospital and in apparently fit men and women. Antibody levels which have commonly been regarded as significant were found in 35 of ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.27.9.722
更新日期：1974-09-01 00:00:00
abstract：:The number and density of argyrophil endocrine cells were morphometrically calculated in gastric fundal mucosal biopsy specimens taken from 64 patients with pernicious anaemia (five with gastric carcinoids, 15 with nodular argyrophil cell hyperplasia, 44 with diffuse argyrophil cell hyperplasia) and from 14 healthy co...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.42.4.371
更新日期：1989-04-01 00:00:00
abstract：AIMS:The Royal College of Pathologists recommend that a median of at least 12 lymph nodes should be harvested during pathological staging of colorectal cancer. It is not always easy to harvest the required number, especially in patients with rectal cancer receiving neoadjuvant therapy. Lymph node revealing solutions, f...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2015-203281
更新日期：2016-06-01 00:00:00
abstract：AIMS:To assess the accuracy of the Mast-ID 15 system compared with API 20 E for the identification of stock and fresh clinical strains of Enterobacteriaceae and Acinetobacter spp; to compare the accuracy of 19 pin and 36 pin multipoint inoculator heads. METHODS:One hundred frozen stock cultures of Enterobacteriaceae a...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.45.2.168
更新日期：1992-02-01 00:00:00
abstract：:The morphology of the labial salivary glands in subjects with and without cystic fibrosis has been studied by light and electron microscopy. The terms ;light' cell, ;dark' cell, ;clear' cell, and ;myoepithelial' cell are used to describe the four cell types distinguished at the level of the electron microscope. The da...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.24.3.270
更新日期：1971-04-01 00:00:00
abstract：:The discovery of cell-free circulating fetal nucleic acids in maternal plasma has opened up new possibilities in non-invasive prenatal diagnosis. The rapid advancement of this field in the past decade is catalysed by the discovery of new classes of fetal nucleic acid markers and technological developments in nucleic a...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.2007.048470
更新日期：2009-04-01 00:00:00
abstract：AIMS:To investigate how the biopsy Gleason score (GS) and the clinical risk classification have been changed by the reporting rules. METHODS:565 prostate biopsy specimens were reassessed. Each Gleason pattern, 1 to 5, was interpreted according to the modified Gleason grading system proposed by the International Societ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2008.060632
更新日期：2009-03-01 00:00:00
abstract：AIMS:To study the pathogenicity and virulence characteristics of Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus sapro-phyticus. METHODS:BALB/c mice were challenged intraperitoneally with graded doses of three strains belonging to each species. LD50s were measured for each strain. Haemolysi...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.47.8.743
更新日期：1994-08-01 00:00:00
abstract：:The biochemical classification of the Klebsiella proposed by Cowan, Steel, Shaw, and Duguid (1960) has been applied to 50 strains isolated as the pure or predominant growth from sputum, and Klebsiella species have been correlated with the severity of the chest infection in the patient. A modification in nomenclature i...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.15.5.478
更新日期：1962-09-01 00:00:00
abstract：:An alternative method for detecting the production of slime by coagulase negative staphylococci was compared with the routinely used Christensen method on 124 isolates of coagulase negative staphylococci from carriage sites, blood cultures, and infected peritoneal dialysis fluids. The alternative method requires the u...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.42.8.872
更新日期：1989-08-01 00:00:00
abstract：:During a 12-month period, 40 146 blood donors were tested for the presence of high titre antitetanus antibodies. An attempt has been made to define the most suitable donors, those with antitetanus antibody titres of 4 IU/ml or above, by virtue of titre, sex, location, and length of time since most recent immunisation...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.30.2.177
更新日期：1977-02-01 00:00:00
abstract：BACKGROUND:Gastric mucosa associated lymphoid tissue (MALT) lymphoma is a low grade B cell lymphoma histologically characterised by neoplastic B cells surrounding follicles in a marginal zone pattern and selectively infiltrating epithelium to form characteristic lymphoepithelial lesions. AIMS:To identify solitary epit...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2004.023747
更新日期：2005-11-01 00:00:00
abstract：:A case of an ovarian serous epithelial neoplasm of borderline type admixed with sarcomatous elements is reported. This combination seems to be extremely rare with only four cases previously reported. It may represent a type of collision tumour or the development of a sarcoma in a growth with borderline differentiation...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.45.3.263
更新日期：1992-03-01 00:00:00
abstract：:Evaluation of histological diagnosis requires an index of agreement (to measure repeatability and validity) together with a method of assessing bias. Cohen's kappa statistic appears to be the most suitable tool for measuring levels of agreement, which if unsatisfactory may be caused by bias. Further study of bias is p...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.36.11.1269
更新日期：1983-11-01 00:00:00
abstract：AIMS:The cell block technique for assessing endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) specimens from pancreatic mucinous cystic lesions (MCLs) was systematically evaluated for the first time, including comparisons with three traditional methods of assessing such specimens. METHODS:The prospective a...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2019-206079
更新日期：2020-02-01 00:00:00
abstract：BACKGROUND:Breast-conserving surgery currently focuses on improving margin clearance and excision volume, the main pathology report parameters for oncological and cosmetic outcomes. AIM:To quantitatively evaluate discrepancies in surgical and pathological estimates of breast specimen sizes, including the influence of ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,多中心研究
doi：10.1136/jclinpath-2011-200392
更新日期：2012-03-01 00:00:00
abstract：:The test is an application of the method originally described by Boyden (1951) and adapted by Stavitsky and Arquilla (1958). Sera which contain anti-Panton-Valentine leucocidin agglutinate the prepared red cells, often to a very high dilution, and this has provided a useful serological test for the diagnosis of staphy...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.14.2.161
更新日期：1961-03-01 00:00:00
abstract：AIMS:Epidermal growth factor receptor (EGFR) T790M mutations can be detected in the circulating tumour DNA from plasma of patients with non-small cell lung cancer (NSCLC) to triage patients for osimertinib eligibility and monitor patients longitudinally for development of T790M-mediated resistance. METHODS:Using dropl...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2020-206668
更新日期：2021-02-01 00:00:00
