Abstract:
AIMS:Epidermal growth factor receptor (EGFR) T790M mutations can be detected in the circulating tumour DNA from plasma of patients with non-small cell lung cancer (NSCLC) to triage patients for osimertinib eligibility and monitor patients longitudinally for development of T790M-mediated resistance. METHODS:Using droplet digital PCR (ddPCR), we examined the EGFR T790M status of 343 sequential patients with NSCLC and correlated mutational status with demographic and clinical features. Where available, serial T790M blood test results were assessed to identify clinical triggers and timing of repeat testing. RESULTS:Of the 343 patients with liquid biopsy test results, 24% were T790M positive. No clear clinical correlation with a T790M positive test result was identified in this study, although the number of metastatic sites did correlate significantly with the presence of EGFR sensitising mutations (L858R or exon 19 deletion) in patient plasma, as a measure of tumour DNA shedding. Of the 59 serial blood tests from patients that initially tested negative, 14% were positive on sequential testing, at a time interval up to 6 months after an initially negative blood test. CONCLUSIONS:The ddPCR test for EGFR T790M mutations effectively triaged 24% of patients for treatment with osimertinib, avoiding the need for invasive tissue biopsy in these patients. Our findings suggest that initial and repeat ctDNA testing can be used to monitor for acquired EGFR T790M resistance for NSCLC.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Spence T,Perera S,Weiss J,Grenier S,Ranich L,Shepherd F,Stockley TLdoi
10.1136/jclinpath-2020-206668subject
Has Abstractpub_date
2021-02-01 00:00:00pages
91-97issue
2eissn
0021-9746issn
1472-4146pii
jclinpath-2020-206668journal_volume
74pub_type
杂志文章abstract::A rapidly fatal case of enteritis necroticans in a 24 year old man with diabetes was caused by Clostridium perfringens type C. The role of beta toxin in the disease is discussed. This type has not been previously described as a causative agent in necrotising bowel disease of man outside endemic areas. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.8.942
更新日期:1984-08-01 00:00:00
abstract::Sections from 100 cervical biopsy specimens were studied by 12 consultant histopathologists to determine the robustness of the existing pathology terminology and classification. Analysis by kappa statistics showed good agreement in the diagnosis of CIN 3 and squamous carcinoma but an inability to distinguish accuratel...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.3.231
更新日期:1989-03-01 00:00:00
abstract::Hepatitis C virus (HCV) replication is reported in both tumour and non-tumour tissue in a case of hepatocellular carcinoma. Viral replication was established by showing the presence of minus strand HCV RNA by PCR amplification, after excluding residual reverse transcriptase activity of Taq polymerase. No minus strand ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.9.880
更新日期:1995-09-01 00:00:00
abstract:AIM:To develop a simple but reliable polymerase chain reaction (PCR) method to determine the HIV-1 status of patients on formalin fixed, paraffin wax embedded lymph node tissue. METHODS:Fifty lymph node specimens, 20 from HIV-1 seropositive and 30 from HIV-1 seronegative patients, were analysed. Lymph nodes with a var...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.8.733
更新日期:1995-08-01 00:00:00
abstract::The mechanism of DNA degradation and its clinical applications were examined. When purified lambda phage and extracted liver DNA were fixed in phosphate buffered formaldehyde, the DNA did not degrade, but there was incomplete digestion with endonuclease. Rat liver tissues were fixed under various conditions and DNA ex...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.9.748
更新日期:1990-09-01 00:00:00
abstract::An immunohistochemical study of the tissue CEA content of 82 epithelial neoplasms of the ovary has shown that mucinous tumours contain more of this substance than do their serous counterparts; otherwise a knowledge of tissue CEA content appears to be of little value in the differential histological diagnosis of this g...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.9.918
更新日期:1979-09-01 00:00:00
abstract::An enzyme linked immunoadsorbent assay (ELISA) was used to detect IgG, IgM, and IgA antibody in urine to a crude antigenic mixture prepared from six arbitrarily chosen strains of common coliform urinary pathogens. In a group of patients with symptoms of urinary tract infection 24 of 80 (30%) urine samples were positiv...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.7.787
更新日期:1987-07-01 00:00:00
abstract::A method of typing isolates of Proteus mirabilis and Proteus vulgaris is described based on the sensitivity of the organisms to bacteriocine. Twelve standard proteocine producing strains were selected from a large number of isolates tested, and from these liquid proteocine preparations were prepared. The sensitivities...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.10.784
更新日期:1975-10-01 00:00:00
abstract::The rate of digestion of fibrin was measured in vitro by an isotopic technique in 140 blood samples of differing fibrinogen concentration; the assessment of fibrinolytic activity thus obtained was compared with a standard method of measurement, the dilute whole blood lysis time. The lysis time was related exponentiall...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.26.3.189
更新日期:1973-03-01 00:00:00
abstract::KRAS mutation status predicts response to anti-EGFR therapy in colorectal cancer patients. Here we report an interesting case of discordant KRAS mutation status in a patient with two separate tumour foci. Tumour A in sigmoid colon invaded through muscularis propria into the subserosal fat with metastatic disease in re...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202591
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:To study the expression of nuclear beta-catenin and Ki-67 in patients with normal gestation products (NGP), complete hydatidiform moles (CHM), and choriocarcinoma to elucidate their roles in carcinogenesis and their interrelations. METHODS:Expression of nuclear beta-catenin and Ki-67 was studied by immunohis...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.026666
更新日期:2006-04-01 00:00:00
abstract::A method is described for assessing thyroid status using Sephadex column chromatography of serum samples with added (125)I triiodothyronine. The method is simple and inexpensive. It gave a correct diagnostic result in 97% of 258 euthyroid, 90% of 80 hyperthyroid, 78% of 59 hypothyroid, and 67% of 50 euthyroid, ill pat...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.20.2.170
更新日期:1967-03-01 00:00:00
abstract:AIMS:To determine whether the recommended methods for the handling of breast excision biopsy specimens from screening patients cause more laboratory work than those used for non-screening patients. METHODS:All breast excision biopsy specimens from 1990 were identified. Ninety one came from patients identified during t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.6.521
更新日期:1992-06-01 00:00:00
abstract::Haemoglobin, haematocrit, and plasma iron values were estimated on blood samples collected from 100 male and 113 female National Blood Transfusion Service blood donors, and from 83 male and 66 female age-matched control subjects. No significant differences in any of these values were found to exist between the donors ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.22.6.680
更新日期:1969-11-01 00:00:00
abstract::Bilateral adrenalectomy produces a reduction in the alkaline phosphatase concentration in rat intestine, which is not prevented by the administration of saline, but is prevented by the administration of cortisone. The administration of A.C.T.H. to normal rats leads to a rise in intestinal alkaline phosphatase levels a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.20.2.185
更新日期:1967-03-01 00:00:00
abstract::A new sensitive nitrite test, the SRN test, was evaluated for its suitability as a reliable screening technique for the detection of bacteriuria. The SRN test was compared to a currently available nitrite test, the Microstix nitrite test, and the results obtained with both nitrite tests were assessed in comparison wit...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.7.723
更新日期:1981-07-01 00:00:00
abstract::A slight modification in tissue processing and staining technique enables a previously described method for staining aluminium to be used to demonstrate aluminium in osteomalacia associated with haemodialysis. The stain appears to be accurate in diagnosing this condition and may assist in establishing the diagnosis be...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.12.1352
更新日期:1981-12-01 00:00:00
abstract::Human papillomavirus antigen was found in 39 (16%) of 253 colposcopic biopsies from a group of women at high risk for cervical cancer who had been examined in the early 1970s. Immunohistochemical evidence of papillomavirus infection was found in 20 (30%) of 67 cervical intraepithelial neoplasia lesions infected with w...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.8.855
更新日期:1985-08-01 00:00:00
abstract::The possible use of sodium hypochlorite solution buffered to pH 7.6 and containing 100 ppm available chlorine (avCl) for disinfecting fibrescopes was investigated. A flexible fibrescope experimentally contaminated with Pseudomonas putida, Mycobacterium fortuitum, or Bacillus subtilis spores was effectively disinfected...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.3.296
更新日期:1982-03-01 00:00:00
abstract::A latex enzyme immunoassay (LEIA) for detecting IgG class antibody to rubella virus was compared with the latex agglutination test (LA) and a haemagglutination inhibition assay (HI). Of 243 sera tested, four discrepant results were observed among all three techniques, corresponding to borderline values. Except for one...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.9.766
更新日期:1990-09-01 00:00:00
abstract:BACKGROUND:Prognostic factors in predicting outcomes in patients with head and neck squamous-cell carcinoma (HNSCC) are limited to the clinical-pathological parameters, including lymph node metastasis, location, grade and stage of the disease. AIM:To determine whether the expression of these proteins has a value in pr...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.036624
更新日期:2007-02-01 00:00:00
abstract:AIMS:To analyse the expression of proteins involved in DNA double strand break detection and repair in the luminal and myoepithelial compartments of benign breast lesions and malignant breast tumours with myoepithelial differentiation. METHODS:Expression of the ataxia telangiectasia (ATM) and p53 proteins was immunohi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.017434
更新日期:2004-11-01 00:00:00
abstract::Muscle biopsy samples were analysed from five control subjects, four patients with mild to moderate fibre atrophy and four patients with severe atrophy. Patchy increase in lipid was noted with oil red O staining but there was no consistent association of lipid with selective fibre types. Ultrastructural studies demons...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.7.778
更新日期:1983-07-01 00:00:00
abstract::Several diseases of the small intestine, including gluten-sensitivity, present with malabsorption and a "flat" mucosa. Determination of the mitotic index of epithelial lymphocytes provides a simple, objective method of assessing, and thus of predicting, whether a flat mucosa is due to gluten-sensitivity (index greater...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.2.149
更新日期:1983-02-01 00:00:00
abstract::The nitroblue tetrazolium (NBT) test has been evaluated as a means of differentiating episodes of infection and rejection in 10 cadaver kidney transplant recipients. Normal NBT values were observed during all 11 episodes of acute rejection. A significant elevation of the NBT score was encountered in six of eight episo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.9.734
更新日期:1974-09-01 00:00:00
abstract::A competitive enzyme linked immunosorbent assay (ELISA) method was used to measure the concentration of lactoferrin in fluids from 36 odontogenic keratocysts, 15 dentigerous cysts, and 30 radicular/residual cysts. Keratocyst fluids contained significantly higher concentrations of lactoferrin than fluids from the other...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.2.180
更新日期:1989-02-01 00:00:00
abstract:AIM:To assess the clinical value of malignancy grading systems compared with nuclear DNA content, protein p53, and angiogenesis for predicting recurrence of stage I (UICC, 1987) tongue carcinomas. METHODS:Histopathological malignancy grading according to Jakobsson and tumour front grading according to Bryne et al were...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.1.35
更新日期:1999-01-01 00:00:00
abstract::Factor VIII procoagulant activity (VIII:C), factor VIII related antigen (VIIIR:AG), and the von Willebrand factor (VIII:WF) were measured in 19 patients with liver disease (8 cases of alcoholic cirrhosis and 11 with acute viral hepatitis). In both groups of patients the levels of VIII:C, VIIIR:AG, and VIIIR:WF were ab...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.3.221
更新日期:1977-03-01 00:00:00
abstract::In a twenty-year period 19 appendicectomy specimens were diagnosed as primary Crohn's disease. This represents 4.9% of the total number of both resection specimens and mucosal biopsies diagnosed as Crohn's disease during that time. This is a review of the main histopathological features found in these appendices and t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.6.632
更新日期:1983-06-01 00:00:00
abstract::A case of an ovarian serous epithelial neoplasm of borderline type admixed with sarcomatous elements is reported. This combination seems to be extremely rare with only four cases previously reported. It may represent a type of collision tumour or the development of a sarcoma in a growth with borderline differentiation...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.3.263
更新日期:1992-03-01 00:00:00