Abstract：
:Hemin is a heme oxygenase-1 (HO-1) inducer which provides endogenous carbon monoxide known for playing roles in cell proliferation, inflammation or aggregation process. The objective of the current study was to examine the effect of prophylactic treatment with hemin in a thrombosis vascular model. Three groups of Wistar rats, control (n = 6), hemin (n = 6) and hemin + HO-1 inhibitor (n = 6), were used for this study. Hemin-treated animals received hemin (50 mg/kg/d; I.P.) for seven days and HO-1 inhibitor group received hemin at the same dose and SnPP IX (60 mg/kg/d; I.P.). All animals were exposed to electric stimulation of the left carotid according to Kawasaki's procedure to induce reproducible thrombus formation. The hemin treatment did not induce blood pressure disturbance. Effects of hemin on vascular thrombosis were quantified by histopathology and its influence on haemostasis was assessed by measuring prothrombin time (PT), activated partial thromboplastin time (APTT) and blood parameters at the end of treatment. The HO-1 mRNA and protein level variation were also checked out. Results showed that chronic treatment with hemin significantly (p < 0.01) reduced the vascular occlusion degree when compared to control and hemin SnPP groups with 7.2 +/- 4.6 vs. 71.1 +/- 14.7 and 74.0 +/- 8.8%, respectively. Moreover, we observed significant (p < 0.05) perturbations of blood parameters in hemin-treated and hemin-SnPP treated rats. Interestingly, hemin treatment did not significantly increase both PT and APTT. Finally, the HO-1 mRNA and protein levels were increased in hemin-treated carotid artery. In conclusion, hemin by inducing HO-1 expression may be a preventive agent against clinical disorders associated to an increased risk of thrombosis events and may limit haemorrhagic risks.
journal_name
Thromb Haemostjournal_title
Thrombosis and haemostasisauthors
Desbuards N,Rochefort GY,Schlecht D,Machet MC,Halimi JM,Eder V,Hyvelin JM,Antier Dsubject
Has Abstractpub_date
2007-09-01 00:00:00pages
614-20issue
3eissn
0340-6245issn
2567-689Xpii
07090614journal_volume
98pub_type
杂志文章abstract：:Our previous study has shown that Toll-like receptor 4 (TLR4) and its signalling pathway contribute to anti-β₂-glycoprotein I/β₂-glycoprotein I (anti-β₂GPI/β₂GPI)-induced tissue factor (TF) expression in human acute monocytic leukaemia cell line THP-1 and annexin A2 (ANX2) is involved in this pathway. However, its dow...
journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：10.1160/TH11-04-0229
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章,评审
doi：10.1160/TH09-05-0310
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：1988-02-25 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章,meta分析
doi：10.1160/TH10-10-0642
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abstract：:Antithrombin (AT) is a serine protease inhibitor that regulates the activity of coagulation proteases of both intrinsic and extrinsic pathways. We identified an AT-deficient patient with a heterozygous Thr90Ser (T90S) mutation who experiences recurrent venous thrombosis. To understand the molecular basis of the clotti...
journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：10.1055/s-0040-1710590
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：1981-02-23 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：1993-05-03 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：10.1160/TH04-03-0165
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：10.1055/a-1344-4405
更新日期：2020-12-31 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：2009-02-01 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章,随机对照试验
doi：10.1160/TH16-09-0728
更新日期：2017-04-03 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：1979-08-31 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：2009-05-01 00:00:00
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journal_title：Thrombosis and haemostasis
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
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journal_title：Thrombosis and haemostasis
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
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doi：10.1160/TH13-04-0320
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
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journal_title：Thrombosis and haemostasis
pub_type： 临床试验,杂志文章
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更新日期：2006-01-01 00:00:00
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章,评审
doi：
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journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：10.1055/s-0039-1692721
更新日期：2019-09-01 00:00:00
abstract：:In this study we sought to evaluate if platelet function measured after percutaneous coronary intervention (PCI) affects the severity of myocardial infarction (MI), measured by markers of cardiac necrosis. We measured platelet function by both a point-of-care assay (PFA-100) and platelet-rich plasma aggregation by two...
journal_title：Thrombosis and haemostasis
pub_type： 杂志文章
doi：
更新日期：2007-10-01 00:00:00
