当前位置： SCI文献检索 > THROMBOSIS AND HAEMOSTASIS期刊下所有文献 > Prolonged exposure to high insulin impairs the endothelial PI3-kinase/Akt/nitric oxide signalling.

Prolonged exposure to high insulin impairs the endothelial PI3-kinase/Akt/nitric oxide signalling.

Abstract：

:Hyperinsulinemia predicts future cardiovascular events, but may also contribute to atherosclerosis. We therefore studied the consequences of prolonged insulin treatment of human umbilical vein endothelial cells (HUVEC) on the phosphatidylinositol-3'-kinase(PI3K)/Akt/nitric oxide(NO)-dependent insulin signaling, together with the expression of the pro-atherogenic molecule vascular cell adhesion molecule (VCAM)-1. HUVEC were incubated with insulin (10(-11) to 10(-7) M) in short- (30 min) and long-term (24 h to 3 days) incubations. In short-term incubations, insulin did not affect constitutive Akt and eNOS at any concentration, but significantly increased their active phosphorylated forms, and NO production. In long-term incubations, however, such insulin effects on the phosphorylated forms, as well as NO production, were attenuated, promoting an effect of insulin also at concentrations otherwise ineffective. Such effects were accompanied by a boosting of insulin effect on VCAM-1 surface expression. In contrast, under similar conditions, insulin did not exert any significant effect on the surface expression of ICAM-1 and E-selectin. Therefore, prolonged exposure of HUVEC to high insulin levels induces a downregulation of the PI3K/Akt/eNOS axis. Such impairment of insulin signalling in states of prolonged hyperinsulinemia pontially contributes to detrimental effects on atherogenesis in insulin resistance states, such as the metabolic syndrome and type 2 diabetes.

journal_name

Thromb Haemost

journal_title

Thrombosis and haemostasis

authors

Madonna R,De Caterina R

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

345-50

issue

2

eissn

0340-6245

issn

2567-689X

pii

09020345

journal_volume

101

pub_type

杂志文章

相关文献

THROMBOSIS AND HAEMOSTASIS文献大全
  • Endothelial perturbation in cystic fibrosis.

    abstract：:Cystic fibrosis (CF) is characterized by a persistent inflammatory state, which can be secondary to chronic pulmonary infection and may affect vascular endothelium. We measured circulating levels of von Willebrand factor (vWF), tissue-plasminogen activator (t-PA), and P-selectin in 20 CF patients and 20 healthy subjec...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Romano M,Collura M,Lapichino L,Pardo F,Falco A,Chiesa PL,Caimi G,Davì G

    更新日期：2001-12-01 00:00:00

  • Immunisation against αIIbβ3 and αvβ3 in a type 1 variant of Glanzmann's thrombasthenia caused by a missense mutation Gly540Asp on β3.

    abstract：:Treatment of bleeding in patients with Glanzmann's thrombasthenia (GT) can be hampered by iso-antibodies against the αIIbβ3 integrin, which cause rapid clearance of transfused donor platelets. Type 1 GT patients with a total absence of αIIbβ3 from the platelet surface are known to be susceptible to form such isoantibo...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH15-12-0982

    authors： Wihadmadyatami H,Röder L,Berghöfer H,Bein G,Heidinger K,Sachs UJ,Santoso S

    更新日期：2016-08-01 00:00:00

  • Vascular thrombogenicity induced by progressive LDL oxidation: protection by antioxidants.

    abstract：:Oxidative modification of LDL, which dysregulates the homeostasis between blood and vascular cells, and alterations of endothelial function are considered among the early events in the pathogenesis of atherosclerosis. This study was designed to evaluate the impact of progressive LDL oxidation on the thrombotic respons...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Banfi C,Camera M,Giandomenico G,Toschi V,Arpaia M,Mussoni L,Tremoli E,Colli S

    更新日期：2003-03-01 00:00:00

  • Pathophysiological relevance of macrophage subsets in atherogenesis.

    abstract：:Macrophages are highly heterogeneous and plastic cells. They were shown to play a critical role in all stages of atherogenesis, from the initiation to the necrotic core formation and plaque rupture. Lesional macrophages primarily derive from blood monocyte, but local macrophage proliferation as well as differentiation...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,评审

    doi：10.1160/TH16-08-0593

    authors： Liberale L,Dallegri F,Montecucco F,Carbone F

    更新日期：2017-01-05 00:00:00

  • Receptor mediated binding of the fibrinolytic components, plasminogen and urokinase, to peripheral blood cells.

    abstract：:Glu-plasminogen binds to platelets; the monocytoid line, U937, and the human fetal fibroblast line, GM1380 bind both plasminogen and its activator, urokinase. This study assesses the interaction of these fibrinolytic proteins with circulating human blood cells. Plasminogen bound minimally to red cells but bound satura...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Miles LA,Plow EF

    更新日期：1987-10-28 00:00:00

  • The platelet adhesion capacity to subendothelial matrix and collagen in a flow model during storage of platelet concentrates for 7 days.

    abstract：:The influence of storage of platelet concentrates (PC) on the adhesion capacity of platelets was studied. Twenty-four PC, 12 prepared by the buffy coat (BC) method and 12 by the platelet-rich plasma (PRP) method, were stored for 7 days at room temperature. On days 1, 3 and 7 of storage, the platelet adhesion capacity ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Boomgaard MN,Gouwerok CW,Homburg CH,de Groot G,IJsseldijk MJ,de Korte D

    更新日期：1994-10-01 00:00:00

  • Effect of urinary protein C inhibitor on lipopolysaccharide-induced disseminated intravascular coagulation in rats.

    abstract：:The effect of urinary protein C inhibitor (uPCI) on disseminated intravascular coagulation (DIC) was investigated using an experimental DIC in rats. uPCI (0.5 and 1.0 mg/kg) was continuously administrated into the left femoral vein of the rats with lipopolysaccharide (50 mg/kg)-induced DIC. In all doses, uPCI signific...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Fujita M,Izutani W,Komurasaki Y

    更新日期：2000-07-01 00:00:00

  • Increased prevalence of hypertension in haemophilia patients.

    abstract：:An increased prevalence of hypertension is reported in haemophilia patients, but data from large, unbiased studies are lacking. The aim of our study was to cross-sectionally assess the prevalence of hypertension in a large cohort of 701 haemophilia patients. Blood pressure (BP) measurements performed in 386 Dutch and ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,多中心研究

    doi：10.1160/TH12-05-0313

    authors： Fransen van de Putte DE,Fischer K,Makris M,Tait RC,Collins PW,Meijer K,Roosendaal G,Chowdary P,Schutgens RE,Mauser-Bunschoten EP

    更新日期：2012-10-01 00:00:00

  • Heavy chain myosin 9-related disease (MYH9 -RD): neutrophil inclusions of myosin-9 as a pathognomonic sign of the disorder.

    abstract：:MYH9-related disease ( MYH9-RD) is an autosomal dominant thrombocytopenia with giant platelets variably associated with young-adult onset of progressive sensorineural hearing loss, presenile cataract, and renal damage. MYH9-RD is caused by mutations of MYH9 , the gene encoding for non-muscle heavy-chain myosin-9. Wild...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH09-08-0593

    authors： Savoia A,De Rocco D,Panza E,Bozzi V,Scandellari R,Loffredo G,Mumford A,Heller PG,Noris P,De Groot MR,Giani M,Freddi P,Scognamiglio F,Riondino S,Pujol-Moix N,Fabris F,Seri M,Balduini CL,Pecci A

    更新日期：2010-04-01 00:00:00

  • Pharmacogenetics of the CD14 endotoxin receptor polymorphism and progression of coronary atherosclerosis.

    abstract：:Atherosclerosis is at least in part an inflammatory disease. CD14 is an endotoxin receptor that after binding of lipopolysaccharides evokes endothelial activation and secretion of several cytokines. A polymorphism of CD14 has been associated with myocardial infarction. We evaluated the role of the -159 T/C polymorphis...

    journal_title：Thrombosis and haemostasis

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1160/TH03-11-0668

    authors： Agema WR,Wouter Jukema J,de Maat MP,Zwinderman AH,Kastelein JJ,Rabelink TJ,van der Wall EE

    更新日期：2004-05-01 00:00:00

  • Dysfunction of annexin A2 contributes to hyperglycaemia-induced loss of human endothelial cell surface fibrinolytic activity.

    abstract：:Hyperglycaemia impairs fibrinolytic activity on the surface of endothelial cells, but the underlying mechanisms are not fully understood. In this study, we tested the hypothesis that hyperglycaemia causes dysfunction of the endothelial membrane protein annexin A2, thereby leading to an overall reduction of fibrinolyti...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH12-12-0944

    authors： Dai H,Yu Z,Fan X,Liu N,Yan M,Chen Z,Lo EH,Hajjar KA,Wang X

    更新日期：2013-06-01 00:00:00

  • Thromboembolic reaction following wall puncture in arterioles and venules of the rabbit mesentery.

    abstract：:The walls of rabbit mesenteric arterioles and venules (diameter 20 to 40 microns) were punctured with glass micropipets (tip diameter 6 to 8 microns). Thromboembolic reactions resulting from this standardized, small mechanical vessel wall injury could be quantified in vivo with the use of intravital video-microscopy. ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： oude Egbrink MG,Tangelder GJ,Slaaf DW,Reneman RS

    更新日期：1988-02-25 00:00:00

  • European Concerted Action on Anticoagulation (ECAA)-the multicentre calibration of rabbit and human ECAA reference thromboplastins. Steering Committee.

    abstract：:Two candidate ECAA reference preparations have been calibrated in a multicentre exercise at 14 representative national laboratories to provide reference thromboplastin for a large field study in 16 European countries. Two preparations were required because of the established differences in ISI arising from the two mai...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,多中心研究

    doi：

    authors： Poller L,Barrowcliffe TW,van den Besselaar AM,Jespersen J,Tripodi A,Houghton D

    更新日期：1996-12-01 00:00:00

  • The sensitivity and specificity of commercial reagents for the detection of lupus anticoagulant show marked differences in performance between photo-optical and mechanical coagulometers.

    abstract：:This study was undertaken to appraise the application of those reagents most widely used in the UK for the detection and confirmation of lupus anticoagulant (LA) on an Amelung KC4A and a Sysmex CA-6000 coagulometer. Five sets of dilute Russell's viper venom time (DRVVT) reagents were assessed as well as the Textarin-P...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Lawrie AS,Mackie IJ,Purdy G,Machin SJ

    更新日期：1999-05-01 00:00:00

  • Low molecular weight heparin for the out-of-hospital treatment of venous thrombosis: rationale and clinical results.

    abstract：:LMWH preparations have been proven to be safe and effective in the out-patient management of acute DVT. Although LMWHs are more expensive than unfractionated heparin, the lack of need for laboratory monitoring of LMWHs and their potential for out-patient treatment more than offsets the drug related cost difference and...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,评审

    doi：

    authors： Hirsh J,Crowther M

    更新日期：1997-07-01 00:00:00

  • Thrombolysis in deep vein thrombosis: is there still an indication?

    abstract：:The most accepted therapy for DVT consists of anticoagulation with unfractionated heparin or low molecular weight heparin, followed by variable duration oral anticoagulation but thrombolytic therapy has been proposed in addition to standard anticoagulation. This paper reviews the literature on post thrombotic syndrome...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,meta分析

    doi：

    authors： Wells PS,Forster AJ

    更新日期：2001-07-01 00:00:00

  • Anti-beta2-glycoprotein I antibodies are associated with pregnancy loss in women with the lupus anticoagulant.

    abstract：:The presence of lupus anticoagulant (LA) predisposes to fetal loss and to venous and arterial thrombosis; however, a subgroup of women is unaffected by pregnancy loss. Currently, no predictive markers are available for the identification of women positive for LA at increased risk for pregnancy loss. It was the aim of ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Sailer T,Zoghlami C,Kurz C,Rumpold H,Quehenberger P,Panzer S,Pabinger I

    更新日期：2006-05-01 00:00:00

  • A multicentre randomised assessment of the DAWN AC computer-assisted oral anticoagulant dosage program.

    abstract：:Computer-assisted oral anticoagulant dosage is being increasingly used to meet growing demands for oral anticoagulation. The DAWN AC is one of the most widely used computer-dosage programs. Evidence of its value and that of other computer programs has been based previously only on laboratory evidence of "time in targe...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：

    authors： Poller L,Keown M,Ibrahim S,Lowe G,Moia M,Turpie AG,Roberts C,van den Besselaar AM,van der Meer FJ,Tripodi A,Palareti G,Shiach C,Bryan S,Samama M,Burgess-Wilson M,Heagerty A,Maccallum P,Wright D,Jespersen J,European

    更新日期：2009-03-01 00:00:00

  • Effect of a synthetic thrombin inhibitor MCI-9038 on experimental models of disseminated intravascular coagulation in rabbits.

    abstract：:We examined the effect of a synthetic thrombin inhibitor, MCI-9038, on two experimental animal models of disseminated intravascular coagulation (DIC). In a model that DIC induced by the intravenous infusion of thrombin, MCI-9038 suppressed the decrease of platelet count by about 50% at a dose of 0.2 micrograms/kg/min ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Hara H,Tamao Y,Kikumoto R,Okamoto S

    更新日期：1987-04-07 00:00:00

  • Role of membrane cholesterol in platelet calcium signalling in response to VWF and collagen under stasis and flow.

    abstract：:Several studies have highlighted a specific role for membrane cholesterol domains in platelet signalling. Upon adhesion to von Willebrand factor (VWF) or collagen, cholesterol-rich domains (CRDs) accumulate in filopodial extensions and selectively harbour counterpart receptors (GPIb and GPVI) and associated signalling...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH07-08-0528

    authors： van Lier M,Verhoef S,Cauwenberghs S,Heemskerk JW,Akkerman JW,Heijnen HF

    更新日期：2008-06-01 00:00:00

  • Studies of alpha-granule proteins in cultured human megakaryocytes.

    abstract：:alpha-Granule protein storage is important for producing platelets with normal haemostatic function. The low to undetectable levels of several megakaryocyte-synthesized alpha-granule proteins in normal plasma suggest megakaryocytes are important to sequester these proteins in vivo. alpha-Granule protein storage in vit...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH03-02-0125

    authors： Veljkovic DK,Cramer EM,Alimardani G,Fichelson S,Massé JM,Hayward CP

    更新日期：2003-11-01 00:00:00

  • A practical, one-clinic visit protocol for pharmacokinetic profile generation with the ADVATE® myPKFiT® dosing tool in severe hemophilia A subjects.

    abstract：:Standard pharmacokinetic (PK) assessments are demanding for persons with hemophilia A, requiring a 72hr washout and 5-11 timed blood samples. A no-washout, single-clinic visit, sparse sampling population PK (PPK) protocol is an attractive alternative. Here, we compared PK parameters obtained with a traditional washout...

    journal_title：Thrombosis and haemostasis

    pub_type： 临床试验

    doi：10.1055/a-1376-0970

    authors： Blanchette V,Zunino L,Grassmann V,Barnes C,Carcao M,Curtain J,Jackson SC,Khoo L,Komrska V,Lillicrap D,Morfini M,Romanova G,Stephens D,Zapotocka E,Rand ML,Blatny J

    更新日期：2021-01-27 00:00:00

  • Long-term secondary prophylaxis in children, adolescents and young adults with von Willebrand disease. Results of a cohort study.

    abstract：:In patients with von Willebrand disease (VWD) replacement therapy with factor VIII/von Willebrand (VWF) concentrates is increasingly applied as prophylactic regimen. Since 2000, 82 consecutively enrolled patients with clinically relevant bleeding episodes (spontaneous, peri- or postoperative) were diagnosed with VWD [...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH10-09-0616

    authors： Halimeh S,Krümpel A,Rott H,Bogdanova N,Budde U,Manner D,Faeser B,Mesters R,Nowak-Göttl U

    更新日期：2011-04-01 00:00:00

  • Vitamin K-dependent protein production in transgenic animals.

    abstract：:Vitamin K-dependent proteins are currently purified from pooled human plasma or produced in mammalian cell culture systems by recombinant DNA technology. Transgenic animals may provide an additional expression system for the production of these therapeutic proteins. Mice have been used to test hybrid genes which direc...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,评审

    doi：

    authors： Luboń H,Paleyanda RK

    更新日期：1997-07-01 00:00:00

  • Vulnerable atherosclerotic plaque metalloproteinases and foam cell phenotypes.

    abstract：:Plaque rupture underlies most myocardial infarctions. Plaques vulnerable to rupture have thin fibrous caps, an excess of macrophages over vascular smooth muscle cells, large lipid cores, and depletion of collagen and other matrix proteins form the cap and lipid core. Production of matrix metalloproteinases from macrop...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Newby AC,George SJ,Ismail Y,Johnson JL,Sala-Newby GB,Thomas AC

    更新日期：2009-06-01 00:00:00

  • Low plasma levels of factor VIIc and antigen are more strongly associated with the 10 base pair promoter (-323) insertion than the glutamine 353 variant.

    abstract：:In a group of 705 healthy middle-aged men, we have examined the relationship between plasma levels of Factor VII (FVII) c and Factor VII antigen (FVIIag) and two polymorphisms in the FVII gene. One polymorphism alters arginine at position 353 to glutamine (R/Q), and the other is the result of a 10 base pair (bp) inser...

    journal_title：Thrombosis and haemostasis

    pub_type： 临床试验,杂志文章

    doi：

    authors： Humphries S,Temple A,Lane A,Green F,Cooper J,Miller G

    更新日期：1996-04-01 00:00:00

  • Factor IX Denver, ASN 346-->ASP mutation resulting in a dysfunctional protein with defective factor VIIIa interaction.

    abstract：:Hemophilia B is a sex-linked recessive bleeding disorder characterized by the presence of either a decreased amount of normal factor IX (FIX) or the presence of a dysfunctional FIX. We have identified a unique mutation in a family with mild hemophilia B. DNA analysis of family members revealed a single base transition...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Lefkowitz JB,Nuss R,Haver T,Jacobson L,Thompson AR,Manco-Johnson M

    更新日期：2001-09-01 00:00:00

  • Measurement of tissue factor messenger RNA levels in leukocytes from patients in hypercoagulable state caused by several underlying diseases.

    abstract：:In a preliminary study, we have demonstrated that tissue factor (TF) is immunohistochemically stained in monocytes from patients with disseminated intravascular coagulation (DIC) but not from healthy volunteers, and that leukocytes from DIC patients induce enhanced activated factor X (FXa) generation in the presence o...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Sase T,Wada H,Nishioka J,Abe Y,Gabazza EC,Shiku H,Suzuki K,Nakamura S,Nobori T

    更新日期：2003-04-01 00:00:00

  • Clot accumulation characteristics of plasminogen-bearing liposomes in a flow-system. Groningen Utrecht Institute for Drug Exploration.

    abstract：:In this study, the clot accumulation properties of liposome-coupled plasminogen were compared to those of free (non-liposomal) plasminogen in an in vitro, closed-loop, flow-system. After introduction of a clot into the closed system, double-radiolabelled plasminogen-liposomes were administered and the accumulation of ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Heeremans JL,Prevost R,Feitsma H,Kluft C,Crommelin DJ

    更新日期：1998-01-01 00:00:00

  • Factor VII Toyama (Thr 359 Met): a homozygous missense mutation causing severe type I deficiency.

    abstract：:We performed a DNA analysis on a patient with severe type I factor VII deficiency by the polymerase chain reaction amplification and a direct DNA sequencing method. The proband was a 66-year-old Japanese woman who had recurrent episodes of excessive bleeding after dental extraction. The functional and antigenic levels...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Ozawa T,Niiya K,Higuchi W,Sakuragawa N

    更新日期：1997-09-01 00:00:00