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The classical and a non-classical pathways associated with NF-kappaB are involved in estrogen-mediated regulation of calbindin-D9k gene in rat pituitary cells.

Abstract：

:Calbindin-D9k (CaBP-9k) is a high affinity calcium binding protein that is highly expressed in the duodenum, kidney, uterus, and pituitary glands. Previous studies have shown that CaBP-9k expression is regulated by several steroid hormones, such as 1,25-dihydroxyvitamin D3, glucocorticoids, 17beta-estradiol (E2), and progesterone (P4), in a tissue-specific manner. However, the promoter elements that mediate transcriptional regulation by these steroid hormones are not clearly understood, mainly due to the lack of an appropriate cell line expressing CaBP-9k. Recently it was shown that CaBP-9k was constitutively expressed in the rat pituitary gland, and is expressed in an E2-dependent manner in a pituitary gland tumor-derived cell line, GH3. In the current study, we examined the activity of the estrogen responsive element (ERE) in rat CaBP-9k gene in GH3 cells, using a luciferase gene reporter assay, electrophoretic mobility shift assay, and mutagenesis. A nuclear factor-kappaB (NF-kappaB) binding site in the CaBP-9k promoter region was identified (nucleotides -848 to -834 from the transcriptional start site), and we demonstrated that addition of an NF-kappaB blocker to GH3 cells reduced E2-induced CaBP-9k transcription. In the present study, we further showed a previously reported imperfect ERE (nucleotides +51 to +65) between exon I and intron A of CaBP-9k, indicating that the interaction of estrogen receptor (ER) alpha with this region is involved in the regulation of CaBP-9k promoter activity and its expression. Taken together, these results suggest that in GH3 cells, both the classical ERalpha-ERE pathway and a non-classical pathway involving NF-kappaB are involved in E2-mediatd regulation of CaBP-9k expression in the pituitary gland.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Lee GS,Choi KC,Han HJ,Jeung EB

doi

10.1016/j.mce.2007.07.009

subject

Has Abstract

pub_date

2007-10-15 00:00:00

pages

42-50

issue

1-2

eissn

0303-7207

issn

1872-8057

pii

S0303-7207(07)00284-5

journal_volume

277

pub_type

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The classical and a non-classical pathways associated with NF-kappaB are involved in estrogen-mediated regulation of calbindin-D9k gene in rat pituitary cells.