Abstract:
:Expression and overexpression of the epidermal growth factor receptor (EGFR) have been described in several solid tumors including bladder, breast, colorectal, NSCLC, prostate, and ovarian cancers. In addition to gene amplification, point mutations within the kinase domain also occur. Previous reports indicate that the patient's response to gefitinib depends on either the presence of mutations within the kinase domain of EGFR or the expression of the most frequent alteration, the truncated EGFR variant III (EGFRvIII). Therefore, it is important to determine if these EGFR alterations are present in urothelial carcinoma. The kinase domain of EGFR (exons 18-21) from 11 bladder cancer cell lines as well as from 75 patient tumors was analyzed by automated sequencing. No mutations were detected in all samples tested. Furthermore, analysis of EGFRvIII by immunohistochemistry revealed that almost half of all the patient samples expressed this truncation in a urothelial carcinoma tissue microarray. However, there have been previous reports of inconsistencies in detecting EGFRvIII by immunohistochemistry owing to the specificity of the antibodies and the methodologies utilized. Therefore, these results were validated by reverse transcription PCR, real-time PCR and western blot analysis. In these assays, none of the samples tested positive for EGFRvIII. Taken together, these results indicate that mutations within the tyrosine kinase domain of EGFR and expression of EGFRvIII are rare events in bladder cancer and therefore do not contribute to the malignant phenotype of this tumor. These results have clinical implications in selecting tyrosine kinase inhibitors for the therapy of urothelial carcinoma.
journal_name
World J Uroljournal_title
World journal of urologyauthors
Villares GJ,Zigler M,Blehm K,Bogdan C,McConkey D,Colin D,Bar-Eli Mdoi
10.1007/s00345-007-0202-7subject
Has Abstractpub_date
2007-12-01 00:00:00pages
573-9issue
6eissn
0724-4983issn
1433-8726journal_volume
25pub_type
杂志文章,收录出版abstract:PURPOSE:The perioperative and oncological outcomes of laparoscopic radical nephrectomy (LRN) for T1-T2 renal cell carcinoma (RCC) are well established. We aim to determine whether LRN is a comparable alternative to open radical nephrectomy (ORN) in the treatment of T3 RCC using a matched pair analysis study design. ME...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-014-1280-y
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:Urinary cytology (C) and cystoscopy remain the gold standard for the detection and screening of bladder cancer (BC). In this prospective study, we analyzed whether baseline C, ImmunoCyt (I), BTA Stat (B), hemoglobin dipstick (H), and NMP22 BladderChek (N) can predict recurrence and progression. METHODS:Urinary...
journal_title:World journal of urology
pub_type: 临床试验,杂志文章
doi:10.1007/s00345-016-1795-5
更新日期:2016-10-01 00:00:00
abstract::Reconstruction of long anterior urethral strictures that cannot be excised and reanastomosed remains controversial. We critically reviewed the literature on free-graft and pedicled skin-flap urethroplasty to determine the optimal method of repair. Overall, free grafts were successful in 84.3% of cases and flaps, in 85...
journal_title:World journal of urology
pub_type: 杂志文章,评审
doi:10.1007/s003450050048
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:We report on a 62-year-old gentleman presenting at our urological department with an advanced renal cell cancer of the right kidney (10 cm in diameter), with an extensive caval vein thrombus (level IV) and bilateral pulmonary metastases. Another suspicious lesion at the left hemithorax was radiologically des...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-012-0955-5
更新日期:2014-02-01 00:00:00
abstract:PURPOSE:Prostate Imaging Reporting and Data System version 2 (PI-RADSv2) provides reasonable performance in detecting significant cancers. Still, it is unclear about whether all PI-RADS 4 lesions show the same cancer detection rate (CDR) regardless of tumor size. The aim was to compare the CDRs of small (< 10 mm) and l...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-019-02924-2
更新日期:2020-06-01 00:00:00
abstract::Urinary bladder cancer (UBC) is a common disease worldwide. At any point in time 2.7 million people have a history of UBC. The incidence of UBC varies over the world with highest rates in developed communities. But the burden of UBC will increase in less developed areas of the world. These changes can be attributed to...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-009-0383-3
更新日期:2009-06-01 00:00:00
abstract::In 90 patients treated with laser prostatectomy using the Urolase (n = 50) or Ultraline (n = 40) laser fiber, the fiber-tip durability was investigated. In general the Urolase fiber tips were less damaged than the Ultraline fiber tips. At visual inspection, 62% of the Urolase fiber tips were graded as minimally damage...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/BF00183619
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVE:To explore efficacy and safety of Botulinum Neurotoxin Type A (BoNT-A) prostatic injection in patients with lower urinary tract symptoms (LUTS) due to benign prostatic hyperperplasia. MATERIALS AND METHODS:A phase 3 multicenter open-labeled study randomised patients to receive BoNT-A prostatic injection or o...
journal_title:World journal of urology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s00345-018-2193-y
更新日期:2018-06-01 00:00:00
abstract::Noncontrast helical computed tomography (CT) has recently been found to be superior to excretory urography (IVU) in the evaluation of patients with suspected ureterolithiasis. Noncontrast helical CT does not require the use of intravenous contrast material with its associated cost and risk of adverse reactions and can...
journal_title:World journal of urology
pub_type: 杂志文章,评审
doi:10.1007/s003450050020
更新日期:1998-01-01 00:00:00
abstract:PURPOSE:Different enhanced recovery after surgery (ERAS) protocols (EP) for radical cystectomy (RC) have been published. Protocols highly differ in number of included items and specific measures. MATERIALS AND METHODS:A systematic review and meta-analysis on EPs in RC were performed using the databases MedLine, Cochra...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-020-03133-y
更新日期:2020-12-01 00:00:00
abstract:PURPOSE:To determine whether individual and/or cumulative components of metabolic syndrome (obesity, hypertension, dyslipidemia, and hyperglycemia) are associated with pathologic features of kidney cancer. PATIENTS AND METHODS:A review of our kidney tumor database identified 462 patients who underwent partial or radic...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-016-1850-2
更新日期:2017-01-01 00:00:00
abstract:INTRODUCTION:Changes in the management of minimally invasive oncologic renal surgery have introduced ablative therapies as the most recent advancement in minimally invasive technology. METHODS:The current evidence-based medicine on the topic of laparoscopic (LCA) and percutaneous (PCA) renal cryoablation was gathered ...
journal_title:World journal of urology
pub_type: 杂志文章,评审
doi:10.1007/s00345-010-0552-4
更新日期:2010-10-01 00:00:00
abstract:PURPOSE:Despite the increasing number of studies confirming the importance of neoadjuvant chemotherapy (NC) in patients before radical cystectomy (RC) for bladder cancer (BCa), NC remains underused. The aim of our study was to develop a nomogram predicting the cancer-specific mortality (CSM) of patients who underwent R...
journal_title:World journal of urology
pub_type: 杂志文章,多中心研究
doi:10.1007/s00345-015-1640-2
更新日期:2016-02-01 00:00:00
abstract::During bladder development, undifferentiated mesenchymal and epithelial cells undergo an orderly sequence of differentiation defined by the expression of smooth-muscle (alpha-actin, myosin, vinculin, desmin, vimentin, and laminin) and epithelial (cytokeratins 5, 7, 8, 14, 18 and 19) protein markers. This process requi...
journal_title:World journal of urology
pub_type: 杂志文章,评审
doi:10.1007/BF00184602
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:To evaluate the association between the plant-based content of diet and serum testosterone levels in men from the national health and nutrition examination survey (NHANES) database. MATERIALS AND METHODS:Data on demographics, diet, and testosterone levels was acquired from the NHANES database. Using the food f...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-020-03276-y
更新日期:2020-05-28 00:00:00
abstract:PURPOSE:To investigate the spontaneous clearance rates of remnant particles following miniaturized percutaneous nephrolithotomy (mini-PCNL) and retrograde intrarenal surgery (RIRS). METHODS:Among 624 patients who underwent mini-PCNL or RIRS at our institution from 2011 to 2015, we collected data of 247 patients with 2...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-016-1807-5
更新日期:2016-11-01 00:00:00
abstract::Although immediate radical cystectomy remains the standard of care for invasive bladder cancer, a large body of international experience from single institutions and cooperative groups has accumulated, suggesting favorable results with bladder-sparing approaches in appropriately selected patients. Modern selective bla...
journal_title:World journal of urology
pub_type: 杂志文章,评审
doi:10.1007/s00345-006-0114-y
更新日期:2006-11-01 00:00:00
abstract:PURPOSE:To compare the ability of Prostate Health Index (PHI) to diagnose csPCa, with that of total PSA, PSA density (PSAD) and the multiparametric magnetic resonance (mpMRI) of the prostate. METHODS:We analysed a group of 395 men planned for a prostate biopsy who underwent a mpMRI of the prostate evaluated using the ...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-020-03397-4
更新日期:2020-08-06 00:00:00
abstract:PURPOSE:Current systemic treatment of targeted therapies, namely the vascular endothelial growth factor-antibody (VEGF-AB), VEGF receptor tyrosine kinase inhibitor (TKI) and mammalian target of rapamycin (mTOR) inhibitors, have improved progression-free survival and replaced non-specific immunotherapy with cytokines in...
journal_title:World journal of urology
pub_type: 杂志文章,评审
doi:10.1007/s00345-016-1868-5
更新日期:2017-02-01 00:00:00
abstract:INTRODUCTION:Urinary incontinence in men after radical prostatectomy affects strongly quality of life. If conservative treatment fails, surgical treatment consists of implantable devices. If the requirement of manual dexterity in the artificial sphincter is to be avoided, the ProACT system offers a readjustable system,...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-018-2483-4
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVES:To characterize testosterone profile changes over time in a cohort of prostate cancer (PCa) patients managed with active surveillance (AS) and to assess its correlation with the initial disease characteristics and further progression. METHODS:We conducted retrospective chart review of PCa patients managed w...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-018-2270-2
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVES:To evaluate retrospectively the objective and subjective parameters in 42 male patients who underwent bone anchored sub-urethral sling positioning (BAUS) for SUI (stress urinary incontinence) due to ISD (intrinsic sphincter deficiency). METHODS:Patients with SUI due to radical retropubic prostatectomy (36 p...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-007-0222-3
更新日期:2008-04-01 00:00:00
abstract::We report on 208 patients with locally advanced renal-cell carcinoma who received a surgical adjuvant vaccination with autologous, Newcastle disease virus (NDV)-modified, and lethally irradiated tumor cells in combination with low-dose recombinant interleukin-2 and interferon-alpha. The pathological stage was defined ...
journal_title:World journal of urology
pub_type: 临床试验,杂志文章
doi:10.1007/BF00184874
更新日期:1995-01-01 00:00:00
abstract:PURPOSE:To evaluate the present human-based evidence that diet is related to the risk and outcome of prostate cancer. METHODS:Review of major case-control and cohort studies, and experimental trials evaluating the effect of diet or dietary constituents on the risk of prostate cancer. RESULTS:Although non-experimental...
journal_title:World journal of urology
pub_type: 杂志文章,评审
doi:10.1007/s00345-011-0810-0
更新日期:2012-04-01 00:00:00
abstract:OBJECTIVES:To evaluate the diagnostic value of 12 core biopsy versus sextant biopsy at different prostatic-specific antigen densities (PSAD). METHODS:We retrospectively analyzed the records of 1,463 patients who underwent transrectal ultrasound-guided prostate biopsies at our institution. 995 patients underwent 12 cor...
journal_title:World journal of urology
pub_type: 杂志文章,多中心研究
doi:10.1007/s00345-008-0264-1
更新日期:2008-08-01 00:00:00
abstract:PURPOSE:This study aims to determine whether intra-network and inter-network brain connectivities are altered using an independent component analysis (ICA). METHODS:Resting-state functional MRI (rs-fMRI) data were acquired from 26 patients with OAB and 28 healthy controls (HC). Eleven resting-state networks (RSNs) wer...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-019-02838-z
更新日期:2020-04-01 00:00:00
abstract:PURPOSE:To describe the clinical characteristics of struvite stones and determine the preoperative predictors of sepsis in struvite patients undergoing percutaneous nephrolithotomy (PCNL). METHODS:A retrospective study of patients who underwent PCNL between April 2011 and March 2018 was performed. The data of the stru...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-019-02748-0
更新日期:2020-01-01 00:00:00
abstract:PURPOSE:To determine whether a delayed percutaneous nephrolithotomy (PCNL) reduces the rate of bacteremia/sepsis in patients with neuromuscular disorders. Patients with neuromuscular disorders are at higher risk of developing complications after PCNL. One strategy to reduce the risk of infectious complications is to pl...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-013-1044-0
更新日期:2013-12-01 00:00:00
abstract:PURPOSE:To assess the impact of prolonged catheterization time on 1-week (short-term), 3-month (intermediate-term) and 1-year (long-term) UC. METHODS:Between 2008 and 2015, 6918 men underwent RP by four high-volume surgeons. Exclusion criteria were baseline urinary incontinence (UI) and radiotherapy prior or within 12...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-018-2274-y
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:Different methods for induction and monitoring of urethral sphincter deficiency were explored in a large animal model. METHODS:Sphincter deficiency was established in female pigs by dilatation and cauterization, and amount and frequencies of voiding were monitored and explored by pad test. Sphincteric closu...
journal_title:World journal of urology
pub_type: 杂志文章
doi:10.1007/s00345-017-2088-3
更新日期:2017-12-01 00:00:00