Abstract：
:One of the key problems in the study of ancient DNA is that of authenticating sequences obtained from PCR amplifications of highly degraded samples. Contamination of ancient samples and postmortem damage to endogenous DNA templates are the major obstacles facing researchers in this task. In particular, the authentication of sequences obtained from ancient human remains is thought by many to be rather challenging. We propose a novel approach, based on the c statistic, that can be employed to help identify the sequence motif of an endogenous template, based on a sample of sequences that reflect the nucleotide composition of individual template molecules obtained from ancient tissues (such as cloned products from a PCR amplification). The c statistic exploits as information the most common form of postmortem damage observed among clone sequences in ancient DNA studies, namely, lesion-induced substitutions caused by cytosine deamination events. Analyses of simulated sets of templates with miscoding lesions and real sets of clone sequences from the literature indicate that the c-based approach is highly effective in identifying endogenous sequence motifs, even when they are not present among the sampled clones. The proposed approach is likely to be of general use to researchers working with DNA from ancient tissues, particularly from human remains, where authentication of results has been most challenging.
journal_name
J Mol Evoljournal_title
Journal of molecular evolutionauthors
Helgason A,Pálsson S,Lalueza-Fox C,Ghosh S,Sigurdardóttir S,Baker A,Hrafnkelsson B,Arnadóttir L,Thorsteinsdóttir U,Stefánsson Kdoi
10.1007/s00239-006-0259-8subject
Has Abstractpub_date
2007-07-01 00:00:00pages
92-102issue
1eissn
0022-2844issn
1432-1432journal_volume
65pub_type
杂志文章abstract：:Here we analyze the molecular evolution of the beta-esterase gene cluster in the Drosophila genus using the recently released genome sequences of 12 Drosophila species. Molecular evolution in this small cluster is noteworthy because it contains contrasting examples of the types and stages of loss of gene function. Spe...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
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更新日期：2009-07-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02101191
更新日期：1988-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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更新日期：2000-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/pl00006381
更新日期：1998-09-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02352300
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/pl00006368
更新日期：1998-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：1999-11-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02100636
更新日期：1986-01-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF01732368
更新日期：1979-12-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-009-9289-3
更新日期：2009-11-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF00173421
更新日期：1994-11-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-003-2543-1
更新日期：2004-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF02101892
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF01653958
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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journal_title：Journal of molecular evolution
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02102318
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pub_type： 杂志文章
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-010-9334-2
更新日期：2010-04-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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更新日期：2004-08-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s002399910031
更新日期：2000-03-01 00:00:00
