Abstract:
:Bacterial lipoproteins, characterized by the N-terminal N-acyl S-diacylglyceryl Cysteine, are key membrane proteins in bacterial homeostasis. It is generally thought that during the modification lipoprotein precursors are translocated via the Sec-machinery in an unfolded state. The recent discovery of twin-arginine translocation (TAT) machinery, meant for exporting folded-proteins, and the presence of TAT-type signal sequences in co-factor-containing (hence already folded) lipoproteins, prompted us to investigate its role and significance in lipoprotein biosynthesis. We systematically analyzed 696 prokaryotic genomes using an algorithm based on DOLOP and TatP rules to predict TAT-pathway-dependent lipoprotein substrates. Occurrence of the deduced TAT-pathway-dependent lipoprotein substrates in relation to genome size, presence or absence of TAT machinery, and extent of its usage for lipoprotein export and habitat types revealed that unlike the host-obligates, the free-living prokaryotes in complex hostile environments (e.g., soil) depend more on TAT-exported lipoproteins. Functional classification of the predicted TAT-dependent lipoproteins revealed enrichment in hydrolases and oxido-reductases, which are fast-folding and co-factor-containing proteins. The role of the TAT pathway in the export of folded-lipoproteins and in niche-specific adaptation for survival has important implications not only in lipoprotein biosynthesis, but also for protein and metabolic engineering applications.
journal_name
J Mol Evoljournal_title
Journal of molecular evolutionauthors
Shruthi H,Babu MM,Sankaran Kdoi
10.1007/s00239-010-9334-2subject
Has Abstractpub_date
2010-04-01 00:00:00pages
359-70issue
4eissn
0022-2844issn
1432-1432journal_volume
70pub_type
杂志文章abstract::Compared with the X chromosome, the mammalian Y chromosome is considerably diminished in size and has lost most of its ancestral genes during evolution. Interestingly, for the X-degenerate region on the Y chromosome, human has retained all 16 genes, while chimpanzee has lost 4 of the 16 genes since the divergence of t...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
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journal_title:Journal of molecular evolution
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journal_title:Journal of molecular evolution
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
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journal_title:Journal of molecular evolution
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journal_title:Journal of molecular evolution
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
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journal_title:Journal of molecular evolution
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
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journal_title:Journal of molecular evolution
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
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pub_type: 杂志文章
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