Detection of hepatitis B virus genotypes using oligonucleotide chip among hepatitis B virus carriers in Eastern China.

Abstract:

AIM:To determine the genotype distribution of hepatitis B virus (HBV) with a newly oligonucleotide chip assay among the HBV carriers in Eastern China. METHODS:An assay using oligonucleotide chip was developed for detection of HBV genotypes in serum samples from HBV DNA-positive patients in Eastern China. This method is based on the principle of reverse hybridization with Cy5-labeled amplicons hybridizing to type-specific oligonucleotide probes that are immobilized on slides. The results of 80 randomly chosen sera were confirmed by direct sequencing. RESULTS:HBV genotype B, C and mixed genotype were detected in 400 serum samples, accounting for 8.3% (n = 33), 83.2% (n = 333), and 8.5% (n = 34), respectively. The evaluation of the oligonucleotide assay showed 100% concordance with the amplicon phylogenetic analysis except 9 mixed genotype infections undetected by sequencing. CONCLUSION:The study indicates that HBV genotype C and B prevail in the Eastern China. It is suggested that the oligonucleotide chip is a reliable and convenient tool for the detection of HBV genotyping.

journal_name

World J Gastroenterol

authors

Tang XR,Zhang JS,Zhao H,Gong YH,Wang YZ,Zhao JL

doi

10.3748/wjg.v13.i13.1975

subject

Has Abstract

pub_date

2007-04-07 00:00:00

pages

1975-9

issue

13

eissn

1007-9327

issn

2219-2840

journal_volume

13

pub_type

杂志文章,多中心研究
  • Entering the duodenal diverticulum: a method for cannulation of the intradiverticular papilla.

    abstract::Successful cannulation of the common bile duct may be difficult in patients in whom the papilla is located entirely within a diverticulum. In this study, we report successful biliary cannulation in three patients following intubation of the distal tip of the duodenoscope into the duodenal diverticulum and locating the...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v18.i48.7394

    authors: Wang BC,Shi WB,Zhang WJ,Gu J,Tao YJ,Wang YQ,Wang XF

    更新日期:2012-12-28 00:00:00

  • Moxibustion inhibits interleukin-12 and tumor necrosis factor alpha and modulates intestinal flora in rat with ulcerative colitis.

    abstract:AIM:To investigate the effect of moxibustion on intestinal flora and release of interleukin-12 (IL-12) and tumor necrosis factor-α (TNF-α) from the colon in rat with ulcerative colitis (UC). METHODS:A rat model of UC was established by local stimulation of the intestine with supernatant from colonic contents harvested...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v18.i46.6819

    authors: Wang XM,Lu Y,Wu LY,Yu SG,Zhao BX,Hu HY,Wu HG,Bao CH,Liu HR,Wang JH,Yao Y,Hua XG,Guo HY,Shen LR

    更新日期:2012-12-14 00:00:00

  • Current treatment for liver metastases from colorectal cancer.

    abstract::The liver is the commonest site of distant metastasis of colorectal cancer and nearly half of the patients with colorectal cancer ultimately develop liver involved during the course of their diseases. Surgery is the only therapy that offers the possibility of cure for patients with hepatic metastatic diseases. Five-ye...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v9.i2.193

    authors: Liu LX,Zhang WH,Jiang HC

    更新日期:2003-02-01 00:00:00

  • Malignant solitary fibrous tumor involving the liver.

    abstract::Solitary fibrous tumors are predominantly benign and are most commonly found in the thoracic cavity and pleura; while reports exist in the literature of malignant solitary fibrous tumors and those located in extrathoracic organs, these cases are considered extremely rare. Herein, a case is reported of a malignant soli...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v19.i21.3354

    authors: Jakob M,Schneider M,Hoeller I,Laffer U,Kaderli R

    更新日期:2013-06-07 00:00:00

  • Comparison of multichannel intraluminal impedance-pH monitoring and reflux scintigraphy in pediatric patients with suspected gastroesophageal reflux.

    abstract:AIM:To evaluate the agreement of multichannel intraluminal impedance-pH monitoring (MII-pHM) and gastroesophageal reflux scintigraphy (GES) for the diagnosis of gastroesophageal reflux disease. METHODS:Seventy-five consecutive patients with suspected gastroesophageal reflux disease (GERD) underwent 24-h combined MII-p...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v22.i43.9595

    authors: Uslu Kızılkan N,Bozkurt MF,Saltık Temizel IN,Demir H,Yüce A,Caner B,Özen H

    更新日期:2016-11-21 00:00:00

  • Prognostic factors in patients with advanced cholangiocarcinoma: role of surgery, chemotherapy and body mass index.

    abstract:AIM:To study the factors that may affect survival of cholangiocarcinoma in Lebanon. METHODS:A retrospective review of the medical records of 55 patients diagnosed with cholangio-carcinoma at the American University of Beirut between 1990 and 2005 was conducted. Univariate and multivariate analyses were performed to de...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.14.3224

    authors: Farhat MH,Shamseddine AI,Tawil AN,Berjawi G,Sidani C,Shamseddeen W,Barada KA

    更新日期:2008-05-28 00:00:00

  • Is endoscopic ultrasonography essential for endoscopic resection of small rectal neuroendocrine tumors?

    abstract:AIM:To evaluate the importance of endoscopic ultrasonography (EUS) for small (≤ 10 mm) rectal neuroendocrine tumor (NET) treatment. METHODS:Patients in whom rectal NETs were diagnosed by endoscopic resection (ER) at the Pusan National University Yangsan Hospital between 2008 and 2014 were included in this study. A tot...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v23.i11.2037

    authors: Park SB,Kim DJ,Kim HW,Choi CW,Kang DH,Kim SJ,Nam HS

    更新日期:2017-03-21 00:00:00

  • Eosinophilic gastroenteritis: clinical experience with 15 patients.

    abstract:AIM:To evaluate the clinic features of eosinophilic gastroenteritis and to examine the diagnosis, treatment, long-term outcome of this disease. METHODS:Charts with a diagnosis of eosinophilic gastroenteritis from 1984 to 2002 at Mackay Memorial Hospital were reviewed retrospectively. There were 15 patients diagnosed w...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v9.i12.2813

    authors: Chen MJ,Chu CH,Lin SC,Shih SC,Wang TE

    更新日期:2003-12-01 00:00:00

  • Green tea polyphenols ameliorate non-alcoholic fatty liver disease through upregulating AMPK activation in high fat fed Zucker fatty rats.

    abstract:AIM:To investigate protective effects and molecular mechanisms of green tea polyphenols (GTP) on non-alcoholic fatty liver disease (NAFLD) in Zucker fatty (ZF) rats. METHODS:Male ZF rats were fed a high-fat diet (HFD) for 2 wk then treated with GTP (200 mg/kg) or saline (5 mL/kg) for 8 wk, with Zucker lean rat as thei...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v23.i21.3805

    authors: Tan Y,Kim J,Cheng J,Ong M,Lao WG,Jin XL,Lin YG,Xiao L,Zhu XQ,Qu XQ

    更新日期:2017-06-07 00:00:00

  • Beneficial effects of adenosine triphosphate-sensitive K+ channel opener on liver ischemia/reperfusion injury.

    abstract:AIM:To investigate the effect of diazoxide administration on liver ischemia/reperfusion injury. METHODS:Wistar male rats underwent partial liver ischemia performed by clamping the pedicle from the medium and left anterior lateral segments for 1 h under mechanical ventilation. They were divided into 3 groups: Control G...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v20.i41.15319

    authors: Nogueira MA,Coelho AM,Sampietre SN,Patzina RA,Pinheiro da Silva F,D'Albuquerque LA,Machado MC

    更新日期:2014-11-07 00:00:00

  • Replication and gene expression of mutant hepatitis B virus in a transgenic mouse containing the complete viral genome with mutant s gene.

    abstract:AIM:To establish the transgenic mouse line harbouring complete hepatitis B virus (HBV) genome with mutant s gene (adr subtype). METHODS:Transgenic mice were generated by microinjecting HBV genome into fertilized eggs. Integration, expression, replication of HBV gene and histological changes in transgenic mice were est...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v10.i21.3141

    authors: Ge JH,Zhang LZ,Li JX,Liu H,Liu HM,He J,Yao YC,Yang YJ,Yu HY,Hu YP

    更新日期:2004-11-01 00:00:00

  • Efficacy of docetaxel combined with oxaliplatin and fluorouracil against stage III/IV gastric cancer.

    abstract:AIM:To investigate the clinical efficacy and toxic effects of neoadjuvant chemotherapy using docetaxel combined with oxaliplatin and fluorouracil for treating stage III/IV gastric cancer. METHODS:A total of 53 stage III/IV gastric cancer patients were enrolled into the study and treated with neoadjuvant chemotherapy. ...

    journal_title:World journal of gastroenterology

    pub_type: 临床试验,杂志文章

    doi:10.3748/wjg.v20.i48.18413

    authors: Yu YJ,Sun WJ,Lu MD,Wang FH,Qi DS,Zhang Y,Li PH,Huang H,You T,Zheng ZQ

    更新日期:2014-12-28 00:00:00

  • Elastography-based screening for esophageal varices in patients with advanced chronic liver disease.

    abstract::Elastography-based liver stiffness measurement (LSM) is a non-invasive tool for estimating liver fibrosis but also provides an estimate for the severity of portal hypertension in patients with advanced chronic liver disease (ACLD). The presence of varices and especially of varices needing treatment (VNT) indicates dis...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v25.i3.308

    authors: Paternostro R,Reiberger T,Bucsics T

    更新日期:2019-01-21 00:00:00

  • Polymorphisms of DNA repair genes XRCC1 and XRCC3, interaction with environmental exposure and risk of chronic gastritis and gastric cancer.

    abstract:AIM:To evaluate the association between polymorphisms XRCC1 Arg194Trp and Arg399Gln and XRCC3 Thr241Met and the risk for chronic gastritis and gastric cancer, in a Southeastern Brazilian population. METHODS:Genotyping by PCR-RFLP was carried out on 202 patients with chronic gastritis (CG) and 160 patients with gastric...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v11.i42.6593

    authors: Duarte MC,Colombo J,Rossit AR,Caetano A,Borim AA,Wornrath D,Silva AE

    更新日期:2005-11-14 00:00:00

  • Insulin-like growth factor binding protein-7 induces activation and transdifferentiation of hepatic stellate cells in vitro.

    abstract:AIM:To investigate the role of insulin-like growth factor binding protein-7 (IGFBP-7) in the activation and transdifferentiation of hepatic stellate cells (HSC) in vitro. METHODS:Rat HSC-T6 cells were cultured in separate dishes and treated with various concentration of transforming growth factor (TGF)-beta(1), IGFBP-...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.15.3246

    authors: Liu LX,Huang S,Zhang QQ,Liu Y,Zhang DM,Guo XH,Han DW

    更新日期:2009-07-14 00:00:00

  • Successful treatment of ileal ulcers caused by immunosuppressants in two organ transplant recipients.

    abstract::Although gastroduodenal ulcers are common in solid organ transplant patients, there are few reports on multiple giant ulcers in the distal ileum and ileocecal valve caused by immunosuppressants Herein, we report on a liver transplant recipient and a renal transplant recipient with multiple large ulcers in the distal i...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v22.i24.5616

    authors: Guo YW,Gu HY,Abassa KK,Lin XY,Wei XQ

    更新日期:2016-06-28 00:00:00

  • Gastric neuroendocrine neoplasms type 1: A systematic review and meta-analysis.

    abstract:BACKGROUND:To date, the histopathological parameters predicting the risk of lymph node (LN) metastases and local recurrence, associated mortality and appropriateness of endoscopic or surgical resection in patients with gastric neuroendocrine neoplasms type 1 (GNENs1) have not been fully elucidated. AIM:To determine th...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,meta分析

    doi:10.3748/wjg.v25.i35.5376

    authors: Tsolakis AV,Ragkousi A,Vujasinovic M,Kaltsas G,Daskalakis K

    更新日期:2019-09-21 00:00:00

  • Systematic review and meta-analysis on the association of tuberculosis in Crohn's disease patients treated with tumor necrosis factor-α inhibitors (Anti-TNFα).

    abstract:AIM:To perform a meta-analysis on the risk of developing Mycobacterium tuberculosis (TB) infection in Crohn's disease (CD) patients treated with tumor necrosis factor-alpha (TNFα) inhibitors. METHODS:A meta-analysis of randomized, double-blind, placebo-controlled trials of TNFα inhibitors for treatment of CD in adults...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,meta分析,评审

    doi:10.3748/wjg.v24.i25.2764

    authors: Cao BL,Qasem A,Sharp RC,Abdelli LS,Naser SA

    更新日期:2018-07-07 00:00:00

  • Improving outcomes in pancreatic cancer: key points in perioperative management.

    abstract::This review focused in the perioperative management of patients with pancreatic cancer in order to improve the outcome of the disease. We consider that the most controversial points in pancreatic cancer management are jaundice management, vascular resection and neo-adjuvant therapy. Preoperative biliary drainage is re...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i39.14237

    authors: Alamo JM,Marín LM,Suarez G,Bernal C,Serrano J,Barrera L,Gómez MA,Muntané J,Padillo FJ

    更新日期:2014-10-21 00:00:00

  • A new framework for reverse cholesterol transport: non-biliary contributions to reverse cholesterol transport.

    abstract::Reduction of low-density lipoprotein-cholesterol through statin therapy has only modestly decreased coronary heart disease (CHD)-associated mortality in developed countries, which has prompted the search for alternative therapeutic strategies for CHD. Major efforts are now focused on therapies that augment high-densit...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v16.i47.5946

    authors: Temel RE,Brown JM

    更新日期:2010-12-21 00:00:00

  • Prominent role of gamma-glutamyl-transpeptidase on the growth of Helicobacter pylori.

    abstract:AIM:gamma-glutamyl transpeptidase (GGT) has been reported as a virulence and colonizing factor of Helicobacter pylori (H pylori). This study examined the effect of GGT on the growth of H pylori. METHODS:Standard H pylori strain NCTC 11637 and 4 clinical isolates with different levels of GGT activity as measured by an ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v10.i20.2994

    authors: Gong M,Ho B

    更新日期:2004-10-15 00:00:00

  • Prognostic value of perioperative leukocyte count in resectable gastric cancer.

    abstract:AIM:To investigate the prognostic significance of perioperative leukopenia in patients with resected gastric cancer. METHODS:A total of 614 eligible gastric cancer patients who underwent curative D2 gastrectomy and adjuvant chemotherapy were enrolled in this study. The relationship between pre- and postoperative hemat...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v22.i9.2818

    authors: Chen XF,Qian J,Pei D,Zhou C,Røe OD,Zhu F,He SH,Qian YY,Zhou Y,Xu J,Xu J,Li X,Ping GQ,Liu YQ,Wang P,Guo RH,Shu YQ

    更新日期:2016-03-07 00:00:00

  • Conventional therapy for Crohn's disease.

    abstract::Crohn's disease (CD) is a multifactorial disorder of unknown cause. Outstanding progress regarding the pathophysiology of CD has led to the development of innovative therapeutic concepts. Numerous controlled trials have been performed in CD over the last years. However, many drugs have not been approved by regulatory ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v12.i30.4794

    authors: Büning C,Lochs H

    更新日期:2006-08-14 00:00:00

  • A novel phenol-bound pectic polysaccharide from Decalepis hamiltonii with multi-step ulcer preventive activity.

    abstract:AIM:To investigate H(+), K(+)-ATPase inhibition, anti-H pylori, antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS:SRPP, with known sugar composition [rhamnose: arabinose: xylose: galactose in the ratio of 16:50:2:32 (w/w), with 141 mg/g ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v13.i39.5196

    authors: Srikanta B,Siddaraju M,Dharmesh S

    更新日期:2007-10-21 00:00:00

  • Current and emerging pharmacological therapy for non-alcoholic fatty liver disease.

    abstract::The main treatment of patients with non-alcoholic fatty liver disease (NAFLD) is life style modification including weight reduction and dietary regimen. Majority of patients are safely treated with this management and pharmacologic interventions are not recommended. However, a subgroup of NAFLD patients with non-alcoh...

    journal_title:World journal of gastroenterology

    pub_type: 社论

    doi:10.3748/wjg.v23.i42.7495

    authors: Eshraghian A

    更新日期:2017-11-14 00:00:00

  • Have patients with esophagitis got an increased risk of adenocarcinoma? Results from a population-based study.

    abstract:AIM:To examine an increased risk of esophageal adenocarcinoma is restricted to patients who develop Barrett's esophagus or whether esophagitis per se is a risk factor for adenocarcinoma. METHODS:A population-based cohort of patients with histological evidence of esophagitis without Barrett's esophagus was constructed ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v11.i46.7290

    authors: Murphy SJ,Anderson LA,Johnston BT,Fitzpatrick DA,Watson PR,Monaghan P,Murray LJ

    更新日期:2005-12-14 00:00:00

  • Metabolic derivatives of alcohol and the molecular culprits of fibro-hepatocarcinogenesis: Allies or enemies?

    abstract::Chronic intake of alcohol undoubtedly overwhelms the structural and functional capacity of the liver by initiating complex pathological events characterized by steatosis, steatohepatitis, hepatic fibrosis and cirrhosis. Subsequently, these initial pathological events are sustained and ushered into a more complex and p...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v22.i1.50

    authors: Boye A,Zou YH,Yang Y

    更新日期:2016-01-07 00:00:00

  • Dietary compliance in celiac disease.

    abstract::Celiac disease is an immune-mediated disorder that causes severe architectural disturbance in the small intestinal mucosa of genetically-predisposed individuals. Impaired absorption of multiple nutrients results and diarrhea and weight loss develop. Evidence has accumulated that a strict gluten-free diet can result in...

    journal_title:World journal of gastroenterology

    pub_type: 社论

    doi:10.3748/wjg.v23.i15.2635

    authors: Freeman HJ

    更新日期:2017-04-21 00:00:00

  • Cancer risk in primary sclerosing cholangitis: Epidemiology, prevention, and surveillance strategies.

    abstract::Primary sclerosing cholangitis (PSC) is a rare cholestatic liver disease characterized by progressive fibroinflammatory destruction of the intra- and/or extrahepatic biliary ducts. While its features and disease course can be variable, most patients with PSC have concurrent inflammatory bowel disease and will eventual...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v25.i6.659

    authors: Fung BM,Lindor KD,Tabibian JH

    更新日期:2019-02-14 00:00:00

  • Oxidative stress as a crucial factor in liver diseases.

    abstract::Redox state constitutes an important background of numerous liver disorders. The redox state participates in the course of inflammatory, metabolic and proliferative liver diseases. Reactive oxygen species (ROS) are primarily produced in the mitochondria and in the endoplasmic reticulum of hepatocytes via the cytochrom...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i25.8082

    authors: Cichoż-Lach H,Michalak A

    更新日期:2014-07-07 00:00:00