当前位置： SCI文献检索 > CHINESE MEDICAL JOURNAL期刊下所有文献 > Clinical application and analysis of hepatitis C virus NS3 antigen detection by ELISA in human serum.

Clinical application and analysis of hepatitis C virus NS3 antigen detection by ELISA in human serum.

Abstract：

BACKGROUND:Hepatitis C virus (HCV) core antigen assays have been produced to exclude infectious donations collected during the preseroconversion window phase (PWP). For the same purpose, we evaluated the specificity and sensitivity of a novel hepatitis C virus NS3 antigen detection immunoassay and the application of this assay in clinical diagnosis. METHODS:Samples from 77 healthy subjects, 173 anti-HCV positive patients and 3708 hepatitis patients other than HCV positive were tested with the HCV NS3 antigen assay. Some HCV NS3 antigen positive samples were further validated with HCV-RNA, neutralization and immunodot assays. Twenty-five sequential samples from 11 HCV NS3 antigen positive patients were subjected to kinetic study. RESULTS:Only 48 (1.3%) of 3708 anti-HCV negative samples were positive for HCV NS3 antigen. Among them, 44 of 3030 samples from patients only infected with HBV were HCV NS3 antigen positive, 4 of the 445 samples from patients infected with other type hepatitis were HCV NS3 antigen positive. In addition, 42 (24.3%) of 173 anti-HCV positive samples were HCV NS3 antigen positive and all 77 samples from healthy subjects were negative to HCV NS3 antigen assay. Of the 15 HCV NS3 antigen positive samples, 9 (60%) were HCV-RNA positive. The neutralization and positive percentage of immunodot assay for 23 HCV NS3 antigen positive sera were 87.0% (20/23) and 69.6% (16/23) respectively. Of the 25 sequential samples from 11 HCV NS3 antigen positive patients, there was a negative correlation between the OD values and the duration of test (r = -0.989, P < 0.05), and there were correlations among their HCV NS3 antigen, HCV-RNA and anti-HCV titres. The anti-HCV antibodies of two sera were detected while their OD values of HCV NS3 antigen decreased gradually. CONCLUSIONS:The HCV NS3 antigen detection assay showed perfect specificity and high sensitivity. Thus, it would be useful and economical as a routine test in laboratories for early diagnosis of HCV infection and prevention.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Xie L,Wu XD,Huang DZ,Chen HL,He LX,Wang J,Han DK

subject

Has Abstract

pub_date

2007-02-20 00:00:00

pages

294-9

issue

eissn

0366-6999

issn

2542-5641

journal_volume

120

pub_type

杂志文章

在线工具

Clinical application and analysis of hepatitis C virus NS3 antigen detection by ELISA in human serum.