Abstract:
:OCT-4 (also known as POU5F1) is a key regulator of self-renewal in embryonic stem cells. Regarding the new cancer stem cell concept, the expression of such genes is potentially correlated with tumorigenesis and can affect some aspects of tumor behavior, such as tumor recurrence or resistance to therapies. Although OCT-4 has been introduced as a molecular marker for germ cell tumors, little is known about its expression in somatic cancers. Here, we have investigated the potential expression of OCT-4 in bladder cancer. We used semiquantitative RT-PCR to examine the expression of OCT-4 in 32 tumors, 13 apparently nontumor tissues taken from the margin of tumors and 9 normal urothelial tissues. The expression of OCT-4 at protein level was further determined by Western blotting and immunohistochemical (IHC) analysis. OCT-4 expression was detected in almost all examined tumors (31/32), but at much lower level (p<0.001) in some nonneoplastic samples (6/22). A significantly strong correlation of 0.6 has been observed between OCT-4 expression and the presence of tumors (p<0.001). Western blot analysis further confirmed the expression of OCT-4 in tumor biopsies. According to IHC results, OCT-4 is primarily localized in the nuclei of tumor cells, with no or low immunoreactivity in nontumor cells. Our study demonstrated, for the first time, the expression of OCT-4 in bladder cancer and a further clue to the involvement of embryonic genes in carcinogenesis.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Atlasi Y,Mowla SJ,Ziaee SA,Bahrami ARdoi
10.1002/ijc.22508subject
Has Abstractpub_date
2007-04-01 00:00:00pages
1598-602issue
7eissn
0020-7136issn
1097-0215journal_volume
120pub_type
杂志文章abstract::Tumour cell anaerobic metabolism has been reported to be a prognostic factor in colorectal cancer. The present study investigated the association between monocarboxylate transporter (MCT) 1, MCT 2, lactate dehydrogenase (LDH) 1 and LDH 5, the tumour microenvironment, and outcome in patients with colorectal cancer. A c...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32045
更新日期:2019-05-01 00:00:00
abstract::The aim of this study was to identify pathways that have a significant impact during renal carcinogenesis. Sixty-seven paired samples of both noncancerous renal cortex tissue and cancerous tissue from patients with clear cell renal cell carcinomas (RCCs) were subjected to whole-exome, methylome and transcriptome analy...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28768
更新日期:2014-09-15 00:00:00
abstract::Endometriosis is a common gynecologic disease, which generally follows a benign course. Notwithstanding, several clinical and histologic studies as well as molecular data show that endometriosis could be a precursor of sporadic endometrioid and clear cell carcinomas at extrauterine loci. Several reports have implicate...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10715
更新日期:2002-12-01 00:00:00
abstract::For many years, clinicians and scientists attempt to develop methods to stimulate the immune system to target malignant cells. Recent data suggest that effective cancer vaccination requires combination immunotherapies to overcome tumor immune evasion. Through presentation of both MHC-I and II molecules, DCs-based vacc...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31795
更新日期:2019-02-15 00:00:00
abstract::Colibactin-producing E. coli (CoPEC) are frequently detected in colorectal cancer (CRC) and exhibit procarcinogenic properties. Because increasing evidence show the role of immune environment and especially of antitumor T-cells in CRC development, we investigated the impact of CoPEC on these cells in human CRC and in ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32920
更新日期:2020-06-01 00:00:00
abstract::Circadian disruption has been classified as probably carcinogenic to humans by the World Health Organization's International Agency for Research on Cancer. The circadian clock is subject to environmental factors, particularly light exposure and food intake rhythms. However, the association between nutritional circadia...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31584
更新日期:2018-11-15 00:00:00
abstract::Esophageal squamous-cell neoplasia (ESCN) is a common second primary neoplasia found in patients with head-and-neck squamous-cell carcinoma (HNSCC). This study sought to identify the risk factors for synchronous ESCN and how they influence survival in HNSCC patient. Eight hundred and fifteen incident HNSCC patients we...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30911
更新日期:2017-11-15 00:00:00
abstract::EBNA-positive lymphoblast cells were detected in 0.1 to 0.9% of the T-cell-depleted lymphocytes obtained from peripheral blood samples of five patients with infectious mononucleosis (IM). The same blood specimens from four of the five patients contained cells that formed EBNA-positive colonies in soft agar containing ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910230603
更新日期:1979-06-15 00:00:00
abstract::Family history of urogenital cancers has been associated with an increased risk of cancer at the same sites. To evaluate the risks of bladder, prostate and renal cell neoplasms for family history of urogenital cancers in first-degree relatives, we analysed data from a case-control study conducted in northern Italy bet...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.23037
更新日期:2007-12-15 00:00:00
abstract::The effect of EGF on the soft agar colony-forming ability of fresh human tumor cells was assessed in 40 specimens obtained from various types of carcinoma including those of the breast, endometrium ovary, and other sites. Cells from four established human tumor cell lines (three breast and one endometrial) were also i...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910300611
更新日期:1982-12-15 00:00:00
abstract::Neoplastic transformation is characterised by an imbalance in favour of cell growth over programmed cell death (apoptosis). The tumour-suppressor gene p53, responsible for maintaining cell-cycle control, is mutated in the majority of human cancers. Loss of function of the target genes of p53 are therefore important in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::Cell lines established from T-cell leukemias have recently been reported to exhibit a chromosome translocation t(8;14) involving proto-oncogene c-myc and the gene of the T-cell receptor alpha-chain(TcR-alpha). In this work, we have studied a case of T-cell leukemia presenting a t(8;14)(q24;q11) translocation that was ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910380609
更新日期:1986-12-15 00:00:00
abstract::We derived from blood lymphocytes of a melanoma patient a large number of cytolytic T-cell clones directed against a cell line of the autologous tumor. Three distinct groups of antigens were recognized by these CTL on the autologous melanoma cells: group A consisted of stable antigens present on all sublines, whereas ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440413
更新日期:1989-10-15 00:00:00
abstract::Heme oxygenase (HO)-1 catalyzes the degradation of cytotoxic heme into biliverdin and blocks antitumor immune responses, thus protecting cancer against host defense. Whether this scenario also applies to neuroblastoma (NB), the most common extracranial solid childhood tumor, is not known. Here, we demonstrate for the ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29933
更新日期:2016-04-15 00:00:00
abstract::Previous studies have shown that macrophage cell lines retained the ability to phagocytize, to secrete lysosomal enzymes, and to function as effector cells in antibody-dependent cellular cytoxicity. In this paper, the cytolytic activity of murine macrophage cell lines against tumor target cells was assessed using an 1...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910260110
更新日期:1980-07-15 00:00:00
abstract::In a series of consecutive 51chromium (51Cr) assays, 21 melanoma and 14 colo-rectal carcinoma patients were tested for their in vitro cell-mediated immune reactions to melanoma, rectal adenocarcinoma, and normal fibroblast target cells. Blood lymphocytes (BL) from four individuals were included in each experiment. In ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910170106
更新日期:1976-01-15 00:00:00
abstract::The family of protein kinases includes many oncogenes and growth-factor receptors, as well as genes that are involved in cell-cycle regulation. We have identified protein kinases expressed in a human breast-cancer cell line, 600PEI, and a primary human breast carcinoma, using PCR cloning techniques based on consensus ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910540409
更新日期:1993-06-19 00:00:00
abstract::Licensed human papillomavirus (HPV) vaccines are expected to prevent high-risk (hr) HPV-infections (most notably types 16 and 18). Whether HPV vaccination will change the distribution of hrHPVs at the population level is open, since competition between HPV types is not well understood. Two stratified random subcohorts...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24539
更新日期:2009-11-01 00:00:00
abstract::Understanding the role of signal transduction in regulating pathways responsible for cell growth, survival and apoptosis is critical for cancer therapy. We developed and characterized a HER2/neu and Fas overexpressing cell line (BNT.888 ACA2) from a salivary gland adenocarcinoma that arose in a HER2/neu transgenic mou...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21837
更新日期:2006-07-15 00:00:00
abstract::Prostate stem cell antigen (PSCA) and prostate-specific antigen (PSA) are overexpressed in most prostate cancers. PSCA- and PSA-derived, HLA-A2 binding peptides are specific targets for T-cell responses in vitro. A phase I/II trial was performed to demonstrate feasibility, safety and induction of antigen-specific immu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22097
更新日期:2006-11-15 00:00:00
abstract::The cytokines that regulate angiogenesis in normal and malignant prostate tissue are not well studied. Using an RT-PCR-based screen, we observed that cultured, low-passage normal human prostate epithelial cells (PrECs) express a variety of cytokines which have been shown to have angiogenic and/or endothelial cell-acti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990315)80:6<868::aid-ijc
更新日期:1999-03-15 00:00:00
abstract::We have shown that modulation of intracellular calcium in EBV latently infected cells could induce the expression of viral antigens, and suggested that a protein kinase-C (PKC) may play a major role in the EBV genome activation. We now report further investigations on the role of PKC using 2 selective enzymatic inhibi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910400624
更新日期:1987-12-15 00:00:00
abstract::Dimethylarsinic acid (DMA) is a major metabolite of inorganic arsenicals, which are epidemiologically significant chemicals in relation to liver cancer in mammals. The present study was conducted to determine the promoting effects of organic arsenicals related to DMA [monomethylarsonic acid (MMA) and trimethylarsine o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10471
更新日期:2002-07-10 00:00:00
abstract::A clone of Cloudman S91 murine melanoma was fused in vitro with non-malignant hamster cheek pouch cells by means of lysolecithin, and the putative hybrid progeny cells, HCP-MM, were found to be highly malignant in hamster, but not in appropriate mice. A malignant clone of HCP-MM cells was shown to have hamster species...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910150214
更新日期:1975-02-15 00:00:00
abstract::Therapeutics targeting the phosphatidylinositol 3-kinase/mammalian target of rapamycin (PI3K/mTOR) pathway initially produce potent antitumor effects, but resistance frequently occurs. Using a phosphoproteome analysis, we found that colorectal cancer (CRC) cells exhibit resistance against PI3K/mTOR inhibition through ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33047
更新日期:2020-11-15 00:00:00
abstract::Risks of 2nd primary cancer were assessed in all patients with cutaneous melanoma (12,460) and all patients with ocular melanoma (2,018) incident in Denmark from 1943 to 1989 and followed for totals of 88,667 person-years and 16,045 person-years, respectively. After cutaneous melanoma, 972 2nd cancers occurred. The ri...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910610606
更新日期:1995-06-09 00:00:00
abstract::Abnormal expression of homeobox genes may lead to the development of leukemias, lymphomas, and solid tumors. Expression of homeobox genes in mammary glands, however, has not been studied actively until recently. We have examined the expression of POU homeobox genes in human breast cancer cell lines and human breast ti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990331)81:1<104::aid-ijc
更新日期:1999-03-31 00:00:00
abstract::The U.S. Preventive Services Task Force (USPSTF) recently updated their national lung screening guidelines and recommended low-dose computed tomography (LDCT) for lung cancer (LC) screening through age 80. However, the risk of overdiagnosis among older populations is a concern. Using four comparative models from the C...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30602
更新日期:2017-06-01 00:00:00
abstract::Vitamin D has anticarcinogenic properties and might influence colorectal cancer (CRC) risk, but the epidemiological evidence is inconsistent. Many mechanisms of action for vitamin D have been proposed, with some of them initiating via its binding to the vitamin D receptor (VDR). Using a large Scottish case-control stu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23769
更新日期:2008-11-01 00:00:00
abstract::The human polyomaviruses JC virus (JCV) and BK virus (BKV) are oncogenic in experimental systems and commonly infect humans. JCV DNA has been reported to be present in human colon mucosa and in colorectal cancers. To investigate whether the risk for colorectal cancer is associated with JCV or BKV infection, we perform...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22770
更新日期:2007-09-01 00:00:00