Abstract:
:The effect of EGF on the soft agar colony-forming ability of fresh human tumor cells was assessed in 40 specimens obtained from various types of carcinoma including those of the breast, endometrium ovary, and other sites. Cells from four established human tumor cell lines (three breast and one endometrial) were also included in this study. The results showed that addition of EGF at a concentration of 50 ng/ml resulted in a 50% higher cloning efficiency in soft agar in 40% of the samples of fresh human tumors. When cells from tumor cell lines were plated in semi-solid medium containing EGF, the number of colonies formed was at least twice as high as controls. Cells from fresh tumor biopsies were assayed for EGF receptors to determine whether the correlation between the proliferative response in EGF-supplemented semi-solid medium as compared to control could be related to the number of EGF receptors present on the cells. Specific receptors for EGF were detected by using radioiodinated EGF in early-passage cell cultures from some of the tumors tested for clonogenicity. The number of receptors ranged from 0.3 to 3.27 X 10(5) per cell. Cells from two melanoma specimens possessed less than 0.3 X 10(3) EGF receptors per cell. We found no correlation between the number of EGF receptor on a cell surface and the mitogenic effect of EGF on the same tumor cells grown in semi-solid medium.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Pathak MA,Matrisian LM,Magun BE,Salmon SEdoi
10.1002/ijc.2910300611subject
Has Abstractpub_date
1982-12-15 00:00:00pages
745-50issue
6eissn
0020-7136issn
1097-0215journal_volume
30pub_type
杂志文章abstract::Female residents of six counties in Washington and Utah in whom a diagnosis of ovarian cancer was made during 1975-79 were interviewed concerning prior use of oral contraceptives. Interviews with a random sample of women drawn from these same counties were obtained for comparison. A smaller proportion of women with ep...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280602
更新日期:1981-12-01 00:00:00
abstract::Epigenetic alterations play a major role in cancer. Recently we reported that stem cell Polycomb group targets (PcGTs) are up to 12-fold more likely to have cancer-specific promoter DNA hypermethylation than nontargets. To identify potential, prognostic DNA methylation markers in ovarian cancer we analyzed the DNA met...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23563
更新日期:2008-08-01 00:00:00
abstract::Oxidative stress plays a role in the regulation of cancer cell metastasis which involves cell invasion and adhesion that could be supported by ADAM proteins through the activities of their metalloprotease and disintegrin domains. We hypothesized that oxidative stress could act through the induction of ADAM9 protein in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25746
更新日期:2011-08-15 00:00:00
abstract::We describe the anatomic and histologic presentation and prognosis of non-Hodgkin's lymphoma (NHL) among people with AIDS (PWA) and determine their contribution to the NHL burden. We linked AIDS and cancer registries in selected areas of the United States and compared NHL sites and histologies in PWA and non-PWA, afte...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19971127)73:5<645::aid-ijc
更新日期:1997-11-27 00:00:00
abstract::We recently reported the identification of a human SART3 gene that encodes a tumor-rejection antigen recognized by cytotoxic T lymphocytes (CTLs). The squamous-cell carcinoma antigen recognized by T cells-3 (SART3) is an RNA-binding protein expressed in the nucleus of the majority of proliferating cells, including nor...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1391
更新日期:2001-09-01 00:00:00
abstract::DNA sequences coding for simian virus 40 (SV40) large T antigen have been detected at different frequencies in human non-Hodgkin's lymphomas (NHL) by PCR techniques as well as immunohistochemistry. A highly sensitive quantitative real-time PCR specific for a sequence of SV40 large T antigen was established to test whe...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21346
更新日期:2006-01-15 00:00:00
abstract::In a series of consecutive 51chromium (51Cr) assays, 21 melanoma and 14 colo-rectal carcinoma patients were tested for their in vitro cell-mediated immune reactions to melanoma, rectal adenocarcinoma, and normal fibroblast target cells. Blood lymphocytes (BL) from four individuals were included in each experiment. In ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910170106
更新日期:1976-01-15 00:00:00
abstract::The autophagy lysosome pathway is essential to maintain cell viability and homeostasis in response to many stressful environments, which is reported to play a vital role in cancer development and therapy. However, the association of genetic alterations of this pathway with risk of cancer remains unclear. Based on geno...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31288
更新日期:2018-07-01 00:00:00
abstract::The mta1 gene is a recently identified novel candidate metastasis-associated gene. The deduced amino acid sequence contains an src homology-3 domain binding motif, a zinc finger motif and possible phosphorylation sites, suggesting that this gene is involved in signal transduction or regulation of gene expression. The ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970822)74:4<459::aid-ijc
更新日期:1997-08-22 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19961211)68:6<822::AID-IJC
更新日期:1996-12-11 00:00:00
abstract::Imbalance in the Kb and Db region encoded molecules is observed in Lewis lung carcinoma clones. The uncloned metastatic population and the D122 high-metastatic clone show no expression of H-2Kb products, while the nonmetastatic A9 clone expresses Kb products. Twenty-nine new subclones of 3LL and A9 were analyzed for D...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910320118
更新日期:1983-07-15 00:00:00
abstract::Embryonal tumors such as Wilms' tumor (WT), embryonal rhabdomyosarcoma (eRMS) and hepatoblastoma have been thought to have a common pathogenetic mechanism. H19 was found to be inactivated in WT and eRMS either by loss of heterozygosity or by hypermethylation of the maternal allele. We show here that the expression of ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990812)82:4<490::aid-ijc
更新日期:1999-08-12 00:00:00
abstract::Despite the steadily increasing number of patients suffering from squamous-cell carcinomas of the oropharyngeal region, little is known about the molecular steps involved in the induction of these neoplasms. We investigated oropharyngeal cancers from 38 patients for mutations in the p53 tumour-suppressor gene. The maj...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910560608
更新日期:1994-03-15 00:00:00
abstract::A panel of 7 diagnostic cytopathologists, i.e., physicians trained to diagnose the malignant potential of human cells in Papanicolaou-stained smears, was asked to evaluate two sets of microscope slides of stained coverslip preparations of 28 cell culture lines, 15 of which were neoplastic. Slide Set I consisted of 13 ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910380310
更新日期:1986-09-15 00:00:00
abstract::Turkey poults were inoculated with lymphoproliferative disease virus (LPDV) of turkey, and the organotropism of the inoculated virus was determined from the kinetics of virus expression and tumor formation. Molecular hybridization experiments, conducted to determine the level of viral RNA expression in the various org...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910290518
更新日期:1982-05-15 00:00:00
abstract::Occupational data from 5 case-control studies in the United States involving 2,973 male cases and 3,210 controls were analyzed to estimate the percentage of lung cancer attributable to well-known and suspected lung carcinogens. The studies were conducted in areas heterogeneous in terms of industrial activities. The pe...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910420610
更新日期:1988-12-15 00:00:00
abstract::Organochlorine insecticides have been studied extensively in relation to breast cancer incidence, and results from two meta-analyses have been null for late-life residues, possibly due to measurement error. Whether these compounds influence survival remains to be fully explored. We examined associations between organo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29806
更新日期:2016-02-01 00:00:00
abstract::Isothiocyanates (ITC) in cruciferous vegetables may be chemopreventive against gastric cancer development. Glutathione S-transferases (GSTs) may modify the chemopreventive effect of ITC. The relationship between urinary total ITC and risk of gastric cancer was prospectively examined. Between 1986 and 1989, 18,244 midd...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24583
更新日期:2009-12-01 00:00:00
abstract::The purpose of this study is to help clarify the use of prostate size as a selection factor for prostate brachytherapy. From 1997 to 1998, 33 patients with a TRUS-based prostate volume greater than 50 cc were treated at the University of Washington by I-125 (144 Gy) or Pd-103 (115 Gy) implantation for prostatic carcin...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-08-20 00:00:00
abstract::There are clear indications that inheritance plays an essential role in certain cases of human endometrial cancer, and there are at least 2 forms of early-onset heritable endometrial adenocarcinomas (EACs). Females of the BDII inbred rat strain are known to be genetically predisposed to endometrial carcinoma, and we h...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1553
更新日期:2001-12-15 00:00:00
abstract::The studies reported here explore the relationship between tumor-specific transplantation antigens (TSTA) and (1) the expression of serologically defined specificities, and (2) the immunogenicity of H-2K- or H-2D-determined alloantigens on methylcholanthrene (MCA)-induced murine fibrosarcomas. Expression of H-2K and H...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910220115
更新日期:1978-07-15 00:00:00
abstract::V79 cells incubated with di- or tetrasulfonated aluminium phthalocyanines (AlPcS2 or AlPcS4) showed a granular fluorescence pattern. Co-staining with the lysosomotropic dye acridine orange (AO) indicated that the granules that were stained by these photoactive phthalocyanines were identical to lysosomes. Small light e...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910580620
更新日期:1994-09-15 00:00:00
abstract::Several observational epidemiological studies have reported inconsistent results on the association between the use of benzodiazepine and the risk of cancer. We investigated the association by using a meta-analysis. We searched PubMed, EMBASE, and the bibliographies of relevant articles to locate additional publicatio...
journal_title:International journal of cancer
pub_type: 杂志文章,meta分析
doi:10.1002/ijc.30443
更新日期:2017-02-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) development entails changes in liver metabolism. Current knowledge on metabolic perturbations in HCC is derived mostly from case-control designs, with sparse information from prospective cohorts. Our objective was to apply comprehensive metabolite profiling to detect metabolites whose se...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33236
更新日期:2021-02-01 00:00:00
abstract::Membrane-type metalloproteinase-1 (MT1-MMP) is a transmembrane metalloproteinase overexpressed in tumors, which plays a major role in the first step of pro-MMP-2 activation, leading to the generation of an intermediate 62 kDa species. The second step of MMP-2 activation that yields to the mature form is less understoo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10134
更新日期:2002-03-01 00:00:00
abstract::Dermatopontin (DPT), a component of the extracellular matrix (ECM), is involved in promotion of cellular adhesion and ECM assembly activities. However, the role of DPT in the pathogenesis of carcinoma is unclear. We evaluated DPT expression in human oral cancer and its possible roles including cellular adhesion and in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26328
更新日期:2012-06-15 00:00:00
abstract::Growing evidence suggests that people with autoimmune conditions may be at increased risk of hepatobiliary tumors. In the present study, we evaluated associations between autoimmune conditions and hepatobiliary cancers among adults aged ≥66 in the United States. We used Surveillance, Epidemiology, and End Results (SEE...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31835
更新日期:2019-02-15 00:00:00
abstract::EBV-specific cytotoxic T cells (Tc) induced in vitro have continuously proliferated in vitro for over 9 months. The long-term maintenance of the Tc growth was dependent on periodic supplementation of both irrediated EBV-transformed autologous lymphoblastoid cell line (LCL) cels and conditioned medium. The latter was d...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280205
更新日期:1981-08-15 00:00:00
abstract::This is a prospective study of lymphoid tissue showing benign reactive hyperplasia (18 lymph nodes and 2 tonsils), using cytogenetic analysis of cells stimulated with T- or B-cell mitogens. The reason for this study was the detection of an abnormal chromosomal population in cells from an enlarged lymph node excised fr...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440602
更新日期:1989-12-15 00:00:00
abstract::Oncogenic activation of MYC occurs often in breast carcinoma and is associated with poor prognosis. Loss or inactivation of mechanisms that restrain MYC may therefore be involved in tumor progression. In this study, we show that the MYC-interacting adaptor protein BIN1 is frequently missing in malignant breast cells a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00