Abstract：
:In a process called quorum sensing, bacteria produce and secrete certain signaling compounds (called autoinducers) that bind to receptors on other bacteria and activate transcription of certain genes. A clever genetic selection yields a new quorum-sensing transcriptional regulator that marches to the beat of a different drummer.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Winans SCdoi
10.1021/cb6003417subject
Has Abstractpub_date
2006-08-22 00:00:00pages
429-31issue
7eissn
1554-8929issn
1554-8937journal_volume
1pub_type
杂志文章,评审abstract：:Glycosylphosphatidylinositol (GPI)-anchored proteins are abundant in the protozoan parasite Trypanosoma brucei, the causative agent of African sleeping sickness in humans and the related disease Nagana in cattle, and disruption of GPI biosynthesis is genetically and chemically validated as a drug target. Here, we exam...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb800143w
更新日期：2008-10-17 00:00:00
abstract：:Complexity generation in naturally occurring peptide scaffolds can occur either by posttranslational modifications of nascent ribosomal proteins or through post assembly line tailoring of nonribosomal peptides. Short enzymatic pathways utilizing bimodular and trimodular nonribosomal peptide synthetase (NRPS) assembly ...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb4001684
更新日期：2013-07-19 00:00:00
abstract：:Epigenetic regulation is directed, in part, by the correlated placement of histone post-translational modifications, but the mechanisms controlling correlated modifications are incompletely understood. Correlations arise from crosstalk among modifications and are frequently attributed to protein-protein interactions t...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb5004527
更新日期：2015-01-16 00:00:00
abstract：:Tumor heterogeneity has hampered the development of novel effective therapeutic options for aggressive cancers, including the deadly primary adult brain tumor glioblastoma (GBM). Intratumoral heterogeneity is partially attributed to the tumor initiating cell (TIC) subset that contains highly tumorigenic, stem-like cel...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00251
更新日期：2018-08-17 00:00:00
abstract：:Labeling of virus opens new pathways for the understanding of viruses themselves and facilitates the utilization of viruses in modern biology, medicine, and materials. Based on the characteristic that viruses hijack their host cellular machineries to survive and reproduce themselves, a host-cell-assisted strategy is p...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb2001878
更新日期：2012-04-20 00:00:00
abstract：:Caenorhabditis elegans lives in compost and decaying fruit, eats bacteria and is exposed to pathogenic microbes. We show that C. elegans is able to modify diverse microbial small-molecule toxins via both O- and N-glucosylation as well as unusual 3'-O-phosphorylation of the resulting glucosides. The resulting glucosyla...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300520u
更新日期：2013-02-15 00:00:00
abstract：:Inducible modulation is often required for precise investigations and manipulations of dynamic biological processes. Transcription activator-like effectors (TALEs) provide a powerful tool for targeted gene editing and transcriptional programming. We designed a series of chemical inducible systems by coupling TALEs wit...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00606
更新日期：2018-03-16 00:00:00
abstract：:GPCRs mediate intracellular signaling upon external stimuli, making them ideal drug targets. However, little is known about their activation mechanisms due to the difficulty in purification. Here, we introduce a method to purify GPCRs in nanodiscs, which incorporates GPCRs into lipid bilayers immediately after membran...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300466n
更新日期：2013-03-15 00:00:00
abstract：:Whereas the C. elegans genome was sequenced many years ago, the role of small molecule signals in its biology is still poorly understood. A recent publication reports the identification of two steroidal signaling molecules that regulate C. elegans reproductive development and dauer diapause via the nuclear receptor DA...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb600173t
更新日期：2006-05-23 00:00:00
abstract：:We have developed a chemically controlled very long-acting delivery system to support once-monthly administration of a peptidic GLP-1R agonist. Initially, the prototypical GLP-1R agonist exenatide was covalently attached to hydrogel microspheres by a self-cleaving β-eliminative linker; after subcutaneous injection in ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00218
更新日期：2017-08-18 00:00:00
abstract：:Protein dimerization provides a mechanism for the modulation of cellular signaling events. In α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors, the rapidly desensitizing, activated state has been correlated with a weakly dimeric, glutamate-binding domain conformation. Allosteric modulators can fo...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb4007166
更新日期：2014-01-17 00:00:00
abstract：:To combat the increasing spread of antimicrobial resistance and the shortage of novel anti-infectives, one strategy for the development of new antibiotics is to optimize known chemical scaffolds. Here, we focus on the biosynthetic engineering of Amycolatopsis sulphurea for derivatization of the atypical tetracycline c...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b01125
更新日期：2019-03-15 00:00:00
abstract：:Recent advances in mass spectrometry (MS)-based proteomics allow the identification and quantitation of thousands of posttranslational modification (PTM) sites in a single experiment. This follows from the development of more effective class enrichment strategies, new high performance instrumentation and bioinformatic...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb500904b
更新日期：2015-01-16 00:00:00
abstract：:The β-hydroxylation of l-histidine is the first step in the biosynthesis of the imidazolone base of the antifungal drug nikkomycin. The cytochrome P450 (NikQ) hydroxylates the amino acid while it is appended via a phosphopantetheine linker to the non-ribosomal peptide synthetase (NRPS) NikP1. The latter enzyme is comp...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00081
更新日期：2017-05-19 00:00:00
abstract：:Calicheamicin γ1I (1) is an enediyne antitumor compound produced by Micromonospora echinospora spp. calichensis, and its biosynthetic gene cluster has been previously reported. Despite extensive analysis and biochemical study, several genes in the biosynthetic gene cluster of 1 remain functionally unassigned. Using a ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb500327m
更新日期：2014-10-17 00:00:00
abstract：:Beta-peptides (beta-amino acid oligomers) that mimic the amphiphilic, helical, and cationic properties of natural antimicrobial peptides have previously been shown to display antifungal activity against planktonic Candida albicans cells. Beta-peptides offer several advantages over conventional peptides composed of alp...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb900093r
更新日期：2009-07-17 00:00:00
abstract：:Large-scale quantification of protein O-linked β- N-acetylglucosamine (O-GlcNAc) modification in a site-specific manner remains a key challenge in studying O-GlcNAc biology. Herein, we developed an isotope-tagged cleavable linker (isoTCL) strategy, which enabled isotopic labeling of O-GlcNAc through bioorthogonal conj...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00414
更新日期：2018-08-17 00:00:00
abstract：:Ammonia lyases catalyze the formation of α,β-unsaturated bonds by the elimination of ammonia from their substrates. This conceptually straightforward reaction has been the emphasis of many studies, with the main focus on the catalytic mechanism of these enzymes and/or the use of these enzymes as catalysts for the synt...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb3002792
更新日期：2012-10-19 00:00:00
abstract：:Protein phosphatases are critical regulators of cellular signaling in both eukaryotes and prokaryotes. The majority of protein phosphatases dephosphorylate phosphoserine/phosphothreonine or phosphotyrosine residues. Recently, however, YwlE, a member of the low-molecular weight protein tyrosine phosphatase (LMW-PTP) fa...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb4001469
更新日期：2013-09-20 00:00:00
abstract：:Aminoglycoside antibiotics were among the first antibiotics discovered and used clinically. Although they have never completely fallen out of favor, their importance has waned due to the emergence of other broad-spectrum antibiotics with fewer side effects. Today, with the dramatically increasing rate of infections ca...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb3005116
更新日期：2013-01-18 00:00:00
abstract：:Nearly one-third of the encoded proteome is comprised of secretory proteins that enable communication between cells and organ systems, playing a ubiquitous role in human health and disease. High-throughput detection of secreted proteins would enhance efforts to identify therapies for secretion-related diseases. Using ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00001
更新日期：2019-03-15 00:00:00
abstract：:Light-activated protein domains provide a convenient, modular, and genetically encodable sensor for optogenetics and optobiology. Although these domains have now been deployed in numerous systems, the precise mechanism of photoactivation and the accompanying structural dynamics that modulate output domain activity rem...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.0c00543
更新日期：2020-10-16 00:00:00
abstract：:A major risk for patients having estrogen receptor α (ERα)-positive breast cancer is the recurrence of drug-resistant metastases after initial successful treatment with endocrine therapies. Recent studies have implicated a number of activating mutations in the ligand-binding domain of ERα that stabilize the agonist co...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00877
更新日期：2018-12-21 00:00:00
abstract：:The nonheme diiron enzyme cyanobacterial aldehyde deformylating oxygenase, cADO, catalyzes the highly unusual deformylation of aliphatic aldehydes to alkanes and formate. We have determined crystal structures for the enzyme with a long-chain water-soluble aldehyde and medium-chain carboxylic acid bound to the active s...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb500343j
更新日期：2014-11-21 00:00:00
abstract：:The mitochondrial ADP/ATP carrier (AAC) is a prominent actor in the energetic regulation of the cell, importing ADP into the mitochondria and exporting ATP toward the cytoplasm. Severe genetic diseases have been ascribed to specific mutations in this membrane protein. How minute, well-localized modifications of the tr...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300012j
更新日期：2012-07-20 00:00:00
abstract：:Our immune system constantly samples peptides found inside the body as a means to detect foreign pathogens, infected cells, and tumorous cells. T cells, which carry out the critical task of distinguishing self from nonself peptides, can only survey peptides that are presented by the major histocompatibility complex pr...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb400594q
更新日期：2013-11-15 00:00:00
abstract：:Transcriptomes provide a myriad of potential RNAs that could be the targets of therapeutics or chemical genetic probes of function. Cell-permeable small molecules, however, generally do not exploit these targets, owing to the difficulty in the design of high affinity, specific small molecules targeting RNA. As part of...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb400265y
更新日期：2013-10-18 00:00:00
abstract：:FAT10 is a ubiquitin-like protein suggested to target proteins for proteasomal degradation. It is highly upregulated upon pro-inflammatory cytokines, namely, TNFα, IFNγ, and IL6, and was found to be highly expressed in various epithelial cancers. Evidence suggests that FAT10 is involved in cancer development and may h...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00667
更新日期：2019-12-20 00:00:00
abstract：:Polyalkoxybenzenes are plant components displaying a wide range of biological activities. In these studies, we synthesized apiol and dillapiol isoxazoline analogues of combretastatins and evaluated their effect on sea urchin embryos. We have shown that p-methoxyphenyl isoxazoline caused sea urchin embryo immobilizatio...
journal_title：ACS chemical biology
pub_type： 信件
doi：10.1021/cb700163q
更新日期：2008-02-15 00:00:00
abstract：:The molecular chaperone Hsp90 requires the assistance of immunophilins, co-chaperones, and partner proteins for the conformational maturation of client proteins. Hsp90 inhibition represents a promising anticancer strategy due to the dependence of numerous oncogenic signaling pathways upon Hsp90 function. Historically,...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb400906e
更新日期：2014-04-18 00:00:00
