Abstract：
:A low concentration of transition metal ions Co2+ and Ni2+ increases the inward current density in neurons from the land snail Helix aspersa. The currents were measured using a single electrode voltage-clamp/internal perfusion method under conditions in which the external Na+ was replaced by Tris+, the predominant external current carrying cation was Ca2+, and the internal perfusate contained 120 mM Cs+/0 K+; 30 mM tetraethylammonium (TEA) was added externally to block K+ current. In the presence of Co2+ (3 mM) or Ni2+ (0.5 mM) inward Ca2+ currents were stimulated normally by voltage-dependent activation of Ca2+ channels. There was a 5-10% decrease in the rate of rise of the inward current. The principal effect of Co2+ and Ni2+ in increasing the current density seems to be a decrease in the rate at which the inward currents decline during a depolarizing voltage pulse. The results may be due to a decrease in a voltage-dependent or Ca(2+)-dependent outward current and/or an inhibition of Ca2+ channel inactivation. Outward current under these conditions (zero internal K+) was significant and most likely due to Cs+ efflux through the voltage-activated or Ca(2+)-activated nonspecific cation channels. Co2+ is an extremely effective blocker of this outward current. These results are not an artifact of internal perfusion or the special ionic conditions. Intracellular recording of unperfused neurons in normal Helix Ringer's solution showed that the Ca(2+)-dependent action potential duration was increased significantly by low concentrations of Co2+. This result is consistant with the Co(2+)-dependent increase in inward (depolarizing) current seen in voltage-clamp experiments.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Kim YK,Woodruff MLdoi
10.1002/jnr.490280411subject
Has Abstractpub_date
1991-04-01 00:00:00pages
549-55issue
4eissn
0360-4012issn
1097-4547journal_volume
28pub_type
杂志文章abstract：:Oxidative stress, neuroinflammation, and excitotoxicity are frequently considered distinct but common hallmarks of several neurological disorders, including Parkinson's disease, amyotrophic lateral sclerosis, multiple sclerosis, and Alzheimer's disease. Although neuron degeneration and death are the ultimate consequen...
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10756
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490110103
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journal_title：Journal of neuroscience research
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更新日期：2008-05-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490180118
更新日期：1987-01-01 00:00:00
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(20000501)60:3<370::AID-JNR
更新日期：2000-05-01 00:00:00
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journal_title：Journal of neuroscience research
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doi：10.1002/jnr.490080237
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journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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更新日期：1992-03-01 00:00:00
