Abstract：
:Ca(2+) influx through L-type Ca(2+) channels regulates several different cellular processes in developing Purkinje neurons, including activation of transcription factors and expression of cellular proteins. In the current studies, we examined the age dependence of these actions of Ca(2+) during the early developmental period. Purkinje neurons acutely isolated from postnatal day 4-8 rat pups were studied. We also examined the sensitivity of the Ca(2+)-regulated processes to a toxic environmental factor (ethanol) known to show age-dependent actions on developing Purkinje neurons. Results show that Ca(2+) activation of the transcription factor cAMP-responsive element binding protein (CREB) and Ca(2+)-induced alterations in the level of the apoptotic enzyme caspase 3 show both dose and age dependence in the early-developing Purkinje neurons. Interestingly, the age dependence was opposite for the two proteins. Ca(2+) regulation of calbindin, a Ca(2+) binding protein, was dose dependent but showed little age dependence. Exposure to ethanol altered Ca(2+) activation of pCREB in an age-dependent manner but did not alter Ca(2+) regulation of caspase 3 or calbindin levels. Taken together, these results show that the downstream effects of Ca(2+) signaling have age-dependent components during early Purkinje neuron development. This age dependence may play an important role in the normal developmental program and could contribute to the critical window of sensitivity observed for certain toxic agents during early development.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Gruol DL,Quina LA,Netzeband JG,Nguyen D,Gullette CEdoi
10.1002/jnr.20844keywords:
subject
Has Abstractpub_date
2006-06-01 00:00:00pages
1381-92issue
8eissn
0360-4012issn
1097-4547journal_volume
83pub_type
杂志文章abstract：:We have previously shown that Na+, K(+)-ATPase activity in hypothalamus is increased after administration of an acute dose of desipramine, a noradrenaline uptake inhibitor (Viola et al., Cell Molec Neurobiol 9:263-271, 1989). In this report the same treatment (10 mg per kg) was applied to evaluate 3H-ouabain binding i...
journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
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更新日期：1992-01-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490220212
更新日期：1989-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-04-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490200103
更新日期：1988-05-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10713
更新日期：2003-09-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490250312
更新日期：1990-03-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490300117
更新日期：1991-09-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19981201)54:5<691::AID-JNR
更新日期：1998-12-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490160123
更新日期：1986-01-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490210107
更新日期：1988-09-01 00:00:00
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journal_title：Journal of neuroscience research
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更新日期：1995-05-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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更新日期：1995-03-01 00:00:00
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journal_title：Journal of neuroscience research
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更新日期：1991-08-01 00:00:00
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journal_title：Journal of neuroscience research
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更新日期：1990-10-01 00:00:00
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更新日期：2000-12-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
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更新日期：2007-08-01 00:00:00
abstract：:Transferrin is the second most abundant plasma protein and functions to transport iron. It is an essential constituent in culture media for virtually all cells. In a recent study, we reported that transferrin (Tf) is specifically located in oligodendrocytes in the rat nervous system. This investigation examines immuno...
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doi：10.1002/jnr.490170108
更新日期：1987-01-01 00:00:00
