SK channels mediate NADPH oxidase-independent reactive oxygen species production and apoptosis in granulocytes.

abstract：:Bacteriorhodopsin from Halobacterium halobium has been solubilized in the nonionic detergent Triton X-100. The circular dichroic spectrum and hydrodynamic properties indicate that the structure of this protein in the detergent is not significantly altered from that of the native membrane-bound form. Bacteriorhodopsin ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reyenolds JA,Stoeckenius W

更新日期：1977-07-01 00:00:00

abstract：:Tumor-promoting phorbol esters affect the ability of mouse hematopoietic progenitor cells to form morphologically recognizable colonies in culture. They induce myeloid progenitor cells to form colonies of the monocyte/macrophage type in the absence of exogenous granulocyte/macrophage colony-stimulating factor. Convers...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sieber F,Stuart RK,Spivak JL

更新日期：1981-07-01 00:00:00

abstract：:Neutron diffraction maps have been used to assign the rotor conformations of the hydroxyl hydrogens in trypsin. Knowledge of these conformations is used to assess the relative importance of steric and electrostatic effects in conferring the H-bonding geometries of these groups. A general finding was that most hydroxyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kossiakoff AA,Shpungin J,Sintchak MD

更新日期：1990-06-01 00:00:00

abstract：:The total synthesis of the sialic acid-containing antigenic epitope fucose GM(1) (Fuc-GM(1)) by an improved reactivity-based one-pot synthetic strategy is reported. Based on a thioglycoside reactivity database, three saccharide building blocks, 3, 4, and 5, were designed and prepared to incorporate a descending order ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mong TK,Lee HK,Duron SG,Wong CH

更新日期：2003-02-04 00:00:00

abstract：:Cyclic nucleotide metabolism was investigated in growing kidneys of rats during compensatory hypertrophy and during neonatal development. After unilateral nephrectomy a mild and short-lasting decrease in cyclic 3':5" adenosine monophosphate (cAMP) was observed in the hypertrophying kidney. In contrast, cyclic 3':5' gu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schlondorff D,Weber H

更新日期：1976-02-01 00:00:00

abstract：:Earlier we have shown that oscillations with a long period ("supercycles") may arise in two-locus systems experiencing cyclical selection with a short period. However, this mode of complex limiting behavior appeared to be possible for narrow ranges of parameters. Here we demonstrate that a multilocus system subjected ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirzhner VM,Korol AB,Nevo E

更新日期：1996-06-25 00:00:00

abstract：:Throughout the world, efforts are under way to restore watersheds, but restoration planning rarely accounts for future climate change. Using a series of linked models of climate, land cover, hydrology, and salmon population dynamics, we investigated the impacts of climate change on the effectiveness of proposed habita...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Battin J,Wiley MW,Ruckelshaus MH,Palmer RN,Korb E,Bartz KK,Imaki H

更新日期：2007-04-17 00:00:00

abstract：:Patients with hematological malignancies can be successfully treated with HLA-matched T cell-depleted allogeneic stem cell transplantation (alloSCT) and subsequent donor lymphocyte infusions (DLIs). The efficacy of DLI is mediated by donor T cells recognizing minor histocompatibility antigens (mHags) on malignant reci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Griffioen M,van der Meijden ED,Slager EH,Honders MW,Rutten CE,van Luxemburg-Heijs SA,von dem Borne PA,van Rood JJ,Willemze R,Falkenburg JH

更新日期：2008-03-11 00:00:00

abstract：:We have shown recently that the temporal order of antioxidant consumption in human blood plasma exposed to a constant flux of aqueous peroxyl radicals is ascorbate = protein thiols greater than bilirubin greater than urate greater than alpha-tocopherol and that detectable lipid peroxidation starts only after ascorbate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frei B,England L,Ames BN

更新日期：1989-08-01 00:00:00

abstract：:A diverse range of neural cell types is generated from a pool of dividing stem and progenitor cells in an orderly manner during development. Little is known of the molecular and cellular biology underpinning the intrinsic control of this process. We have used a nonbiased method to purify populations of neural progenit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Livesey FJ,Young TL,Cepko CL

更新日期：2004-02-03 00:00:00

abstract：:It is unknown whether cross-regulatory interactions between homeotic genes, which have been shown to play an important role in the maintenance of their expression domains during Drosophila development, are also important during mammalian development. We have analyzed here the expression of Hox genes in Hoxa-1 (Hox-1.6...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dollé P,Lufkin T,Krumlauf R,Mark M,Duboule D,Chambon P

更新日期：1993-08-15 00:00:00

abstract：:Six groups of Munich-Wistar rats underwent micropuncture study 2-10 weeks and morphologic studies 11-13 months after induction of streptozotocin diabetes or after sham treatment. Diabetic rats received diets containing 6% (group D6), 12% (D12), or 50% protein (D50) and were maintained under similar conditions of moder...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zatz R,Meyer TW,Rennke HG,Brenner BM

更新日期：1985-09-01 00:00:00

abstract：:A somatic hybrid of ASL-1 leukemia cells [H-2a, thymus leukemia (TL)1,2,3, Thy-1b] and LM(TK)-cells [H-2-k, TL(-), Thy-1 (-), thymidine kinase deficient] was formed with the aid of inactivated Sendai virus. The selection of hybrid cell clones was facilitated by the inability of ASL-1 cells to grow in vitro and of LM(T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang W,Cohen EP

更新日期：1975-05-01 00:00:00

abstract：:YC-1 [3-(5'-hydroxymethyl-2'furyl)-1-benzyl indazole] is an allosteric activator of soluble guanylyl cyclase (sGC). YC-1 increases the catalytic rate of the enzyme and sensitizes the enzyme toward its gaseous activators nitric oxide or carbon monoxide. In other studies the administration of YC-1 to experimental animal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martin E,Lee YC,Murad F

更新日期：2001-11-06 00:00:00

abstract：:The 1,3-1,4-beta-glucanase from Bacillus macerans (wtGLU) and the 1, 4-beta-xylanase from Bacillus subtilis (wtXYN) are both single-domain jellyroll proteins catalyzing similar enzymatic reactions. In the fusion protein GluXyn-1, the two proteins are joined by insertion of the entire XYN domain into a surface loop of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aÿ J,Götz F,Borriss R,Heinemann U

更新日期：1998-06-09 00:00:00

abstract：:The inference of the evolutionary history of a set of languages is a complex problem. Although some languages are known to be related through descent from common ancestral languages, for other languages determining whether such a relationship holds is itself a difficult problem. In this paper we report on new methods,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warnow T

更新日期：1997-06-24 00:00:00

abstract：:Throughout three domains of life, alanyl-tRNA synthetases (AlaRSs) recognize a G3:U70 base pair in the acceptor stem of tRNAAla as the major identity determinant of tRNAAla The crystal structure of the archaeon Archaeoglobus fulgidus AlaRS in complex with tRNAAla provided the basis for G3:U70 recognition with residues...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chong YE,Guo M,Yang XL,Kuhle B,Naganuma M,Sekine SI,Yokoyama S,Schimmel P

更新日期：2018-07-17 00:00:00

abstract：:Stromal cell lines were established from canine long-term marrow cultures, cloned by limiting dilution, and maintained in stromal cell-conditioned medium. These cells grew adherent, maintained stable growth rate and morphology under standard conditions (in 20-30% conditioned medium; confluency, 70-90%), and supported ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huss R,Hong DS,McSweeney PA,Hoy CA,Deeg HJ

更新日期：1995-01-31 00:00:00

abstract：:Numerous studies have shown that high olive oil intake reduces blood pressure (BP). These positive effects of olive oil have frequently been ascribed to its minor components, such as alpha-tocopherol, polyphenols, and other phenolic compounds that are not present in other oils. However, in this study we demonstrate th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Terés S,Barceló-Coblijn G,Benet M,Alvarez R,Bressani R,Halver JE,Escribá PV

更新日期：2008-09-16 00:00:00

abstract：:The Notch signaling pathway defines an evolutionarily conserved cell-cell interaction mechanism that throughout development controls the ability of precursor cells to respond to developmental signals. Here we show that Notch signaling regulates the expression of the master control genes eyeless, vestigial, and Distal-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kurata S,Go MJ,Artavanis-Tsakonas S,Gehring WJ

更新日期：2000-02-29 00:00:00

abstract：:The SRC gene is the prototype for a family of closely related genes whose products have protein-tyrosine kinase activity. We recently described another member of this family, designated FYN, whose cDNA was isolated from normal human fibroblasts. To examine the possible role of FYN as an oncogene, we investigated the e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawakami T,Kawakami Y,Aaronson SA,Robbins KC

更新日期：1988-06-01 00:00:00

abstract：:Climate change is expected to cause shifts in the composition of tropical montane forests towards increased relative abundances of species whose ranges were previously centered at lower, hotter elevations. To investigate this process of "thermophilization," we analyzed patterns of compositional change over the last de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duque A,Stevenson PR,Feeley KJ

更新日期：2015-08-25 00:00:00

abstract：:Metabolic crossfeeding is an important process that can broadly shape microbial communities. However, little is known about specific crossfeeding principles that drive the formation and maintenance of individuals within a mixed population. Here, we devised a series of synthetic syntrophic communities to probe the comp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mee MT,Collins JJ,Church GM,Wang HH

更新日期：2014-05-20 00:00:00

abstract：:Elucidation of mechanisms that regulate hematopoietic stem cell self-renewal and differentiation would be facilitated by the identification of defined culture conditions that allow these cells to be amplified. We now demonstrate a significant net increase (3-fold, P < 0.001) in vitro of cells that are individually abl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller CL,Eaves CJ

更新日期：1997-12-09 00:00:00

abstract：:The rpoN gene encoding the transcription factor sigma54 in Myxococcus xanthus has been cloned using a heterologous rpoN probe. The sequence of the cross-hybridizing DNA confirmed the existence of an ORF 1518 bp long that encodes a well conserved member of the sigma54 family of sigma factors. Low- as well as high-strin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keseler IM,Kaiser D

更新日期：1997-03-04 00:00:00

abstract：:We have studied the involvement of the 5' cap structure in the splicing of precursor mRNAs in a HeLa nuclear extract. We show that precursor mRNAs are spliced efficiently only when they possess a cap structure and that preincubation of a HeLa nuclear extract rendered the splicing reaction highly sensitive to inhibitio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edery I,Sonenberg N

更新日期：1985-11-01 00:00:00

abstract：:Toll-like receptors (TLRs) have been shown to participate in the recognition of pathogens by the innate immune system, but it is not clear how a restricted family of receptors has the capacity to recognize the wide spectrum of TLR stimuli known to exist. We report here that two members of the TLR family, TLR2 and TLR6...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ozinsky A,Underhill DM,Fontenot JD,Hajjar AM,Smith KD,Wilson CB,Schroeder L,Aderem A

更新日期：2000-12-05 00:00:00

abstract：:Rhomboid domain-containing protein 3 (Rhbdd3), which belongs to a family of proteins with rhomboid domain, is widely expressed in immune cells; however, the roles of the Rhbdd members, including Rhbdd3, in immunity remain unknown. Natural killer (NK) cells are critical for host immune defense and also can mediate infl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Liu S,Xia M,Xu S,Wang C,Bao Y,Jiang M,Wu Y,Xu T,Cao X

更新日期：2013-05-07 00:00:00

abstract：:After males of Lasioglossum zephyrum (Hymenoptera: Halictidae) have first been exposed to a conspecific female, the attractiveness of a second female diminishes with increasing genealogical relationship between the two females. The most parsimonious explanation for this is a polygenically controlled female sex pheromo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith BH

更新日期：1983-07-01 00:00:00

abstract：:More than 21 million prescriptions for warfarin are written yearly in the U.S. Despite its importance, warfarin's target, vitamin K epoxide reductase (VKOR), has resisted purification since its identification in 1972. Here, we report its purification and reconstitution. HPC4, a calcium-specific antibody that recognize...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chu PH,Huang TY,Williams J,Stafford DW

更新日期：2006-12-19 00:00:00