Expressional potency of mRNAs encoding receptors and voltage-activated channels in the postmortem rat brain.

abstract：:By using titin as a model system, we have demonstrated that fluorescence quenching can be used to study protein folding at the single molecule level. The unfolded titin molecules with multiple dye molecules attached are able to fold to the native state. In the native folded state, the fluorescence from dye molecules i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhuang X,Ha T,Kim HD,Centner T,Labeit S,Chu S

更新日期：2000-12-19 00:00:00

abstract：:The major small nuclear ribonucleoproteins (snRNPs) U1, U2, U4/U6, and U5 share a set of common proteins denoted B/B', D1, D2, D3, E, F, and G which play an important part in the biogenesis of the snRNPs. In addition, there is a link between the common proteins and autoimmunity; the three D proteins, together with B/B...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehmeier T,Raker V,Hermann H,Lührmann R

更新日期：1994-12-06 00:00:00

abstract：:CD4-expressing T cells in lymphoid organs are infected by the primary strains of HIV and represent one of the main sources of virus replication. Gene therapy strategies are being developed that allow the transfer of exogenous genes into CD4(+) T lymphocytes whose expression might prevent viral infection or replication...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schnierle BS,Stitz J,Bosch V,Nocken F,Merget-Millitzer H,Engelstädter M,Kurth R,Groner B,Cichutek K

更新日期：1997-08-05 00:00:00

abstract：:Chronic psychosocial stress/trauma represents an increasing burden in our modern society and a risk factor for the development of mental disorders, including posttraumatic stress disorder (PTSD). PTSD, in turn, is highly comorbid with a plethora of inflammatory disorders and has been associated with increased bone fra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haffner-Luntzer M,Foertsch S,Fischer V,Prystaz K,Tschaffon M,Mödinger Y,Bahney CS,Marcucio RS,Miclau T,Ignatius A,Reber SO

更新日期：2019-04-23 00:00:00

abstract：:Adeno-associated virus (AAV) is a small human Dependovirus whose low immunogenicity and capacity for long-term persistence have led to its widespread use as vector for gene therapy. Despite great recent successes in AAV-based gene therapy, further improvements in vector technology may be hindered by an inadequate unde...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith-Moore S,Neil SJD,Fraefel C,Linden RM,Bollen M,Rowe HM,Henckaerts E

更新日期：2018-04-10 00:00:00

abstract：:Retinopathy of prematurity is a blinding disease, initiated by lack of retinal vascular growth after premature birth. We show that lack of insulin-like growth factor I (IGF-I) in knockout mice prevents normal retinal vascular growth, despite the presence of vascular endothelial growth factor, important to vessel devel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hellstrom A,Perruzzi C,Ju M,Engstrom E,Hard AL,Liu JL,Albertsson-Wikland K,Carlsson B,Niklasson A,Sjodell L,LeRoith D,Senger DR,Smith LE

更新日期：2001-05-08 00:00:00

abstract：:The human rhodopsin gene is the locus for numerous alleles linked to the neurodegenerative disease retinitis pigmentosa. To facilitate the study of retinal degeneration and to test reagents designed to alter the structure and function of this gene, we have developed strains of mice whose native rhodopsin gene has been...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan F,Bradley A,Wensel TG,Wilson JH

更新日期：2004-06-15 00:00:00

abstract：:Speciation involves the origin of trait differences that limit or prevent gene exchange and ultimately results in daughter populations that form monophyletic or exclusive genetic groups. However, for recently diverged populations or species between which reproductive isolation is often incomplete, gene genealogies wil...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dopman EB,Pérez L,Bogdanowicz SM,Harrison RG

更新日期：2005-10-11 00:00:00

abstract：:Mutations arise in a single individual and at a single point in time and space. The geographic distribution of mutations reflects both historical population size and frequency of migration. We employ coalescence-based methods to coestimate effective population size, frequency of migration, and level of recombination c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrell PL,Lundy KE,Clegg MT

更新日期：2003-09-16 00:00:00

abstract：:During cell division, the mitotic spindle segregates the sister chromatids into two nascent cells, such that each daughter cell inherits one complete set of chromosomes. Errors in spindle formation can result in both chromosome missegregation and cytokinesis defects and hence lead to genomic instability. To ensure the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nousiainen M,Silljé HH,Sauer G,Nigg EA,Körner R

更新日期：2006-04-04 00:00:00

abstract：:Reports differ as to whether reconstitution of telomerase activity alone is sufficient for immortalization of different types of human somatic cells or whether additional activities encoded by other "immortalizing" genes are also required. Here we show that ectopic expression of either the catalytic subunit of human t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Hare MJ,Bond J,Clarke C,Takeuchi Y,Atherton AJ,Berry C,Moody J,Silver AR,Davies DC,Alsop AE,Neville AM,Jat PS

更新日期：2001-01-16 00:00:00

abstract：:We have developed a gene-fusion system based on the Escherichia coli beta-glucuronidase gene (uidA). The uidA gene has been cloned from E. coli K-12 and its entire nucleotide sequence has been determined. beta-Glucuronidase has been purified to homogeneity and characterized. The enzyme has a subunit molecular weight o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jefferson RA,Burgess SM,Hirsh D

更新日期：1986-11-01 00:00:00

abstract：:gamma-Aminobutyric acid (GABA)-modulin is a brain protein of Mr 16,500 that down-regulates the high-affinity binding site for GABA which is located in crude synaptic membranes. This protein can be phosphorylated in vitro by the catalytic subunit of cAMP-dependent protein kinase and by a partially purified preparation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wise BC,Guidotti A,Costa E

更新日期：1983-02-01 00:00:00

abstract：:Very-long-baseline interferometry images of the nuclear region of the radio galaxy Cygnus A reveal a pronounced "core" and a knotty jet and counterjet. The knots are moving away from the core at apparent speeds which are subluminal for h = 1 [h = H0/100 km.s-1.Mpc-1;1 parsec (pc) = 3.09 x 10(16)m] and about c for h = ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bartel N,Sorathia B,Bietenholz MF,Carilli CL,Diamond P

更新日期：1995-12-05 00:00:00

abstract：:Although biological cells are mostly transparent, they are phase objects that differ in shape and refractive index. Any image that is projected through layers of randomly oriented cells will normally be distorted by refraction, reflection, and scattering. Counterintuitively, the retina of the vertebrate eye is inverte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Franze K,Grosche J,Skatchkov SN,Schinkinger S,Foja C,Schild D,Uckermann O,Travis K,Reichenbach A,Guck J

更新日期：2007-05-15 00:00:00

abstract：:The present study examined the social skills of previously institutionalized, 8-y-old Romanian children from the Bucharest Early Intervention Project and the influence of attachment security and brain electrical activity (alpha power) on these skills. Participants included children randomized to an intervention involv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Almas AN,Degnan KA,Radulescu A,Nelson CA 3rd,Zeanah CH,Fox NA

更新日期：2012-10-16 00:00:00

abstract：:Prediction of the native tertiary structure of a globular protein from the primary sequence will require a potential energy model that can discriminate all nonnative structures from the native structure(s). A successful model must distinguish not only alternate structures that are very nonnative but also alternate str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Zhang H,Li W,Scott RA

更新日期：1995-01-31 00:00:00

abstract：:Physiological sensing of O(2) tension (partial O(2) pressure, pO(2)) plays an important role in some mammalian cellular systems, but striated muscle generally is not considered to be among them. Here we describe a molecular mechanism in skeletal muscle that acutely couples changes in pO(2) to altered calcium release t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun QA,Hess DT,Nogueira L,Yong S,Bowles DE,Eu J,Laurita KR,Meissner G,Stamler JS

更新日期：2011-09-20 00:00:00

abstract：:It has been recognized since ancient times that malaria fever is highly periodic but the mechanism has been poorly understood. Malaria fever is related to the parasite growth cycle in erythrocytes. After a fixed period of replication, a mature parasite (schizont) causes the infected erythrocyte to rupture, releasing p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kwiatkowski D,Nowak M

更新日期：1991-06-15 00:00:00

abstract：:The nucleotide sequence of a 1,163-base-pair fragment that encodes the entire thyA gene of Escherichia coli K-12 was determined. The strategy involved sequence determination of both DNA strands by using overlapping deletions that had been generated in vitro from the two ends of the fragment with BAL-31 nuclease. The a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Belfort M,Maley G,Pedersen-Lane J,Maley F

更新日期：1983-08-01 00:00:00

abstract：:A series of spontaneous suppressors to the temperature-sensitive phenotype of the single-stranded DNA-binding protein mutation ssb-1 were isolated. A genomic library of EcoRI fragments from one of these suppressor strains was prepared by using pBR325 as the cloning vector. A 10.0-kilobase class of inserts was identifi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruben SM,VanDenBrink-Webb SE,Rein DC,Meyer RR

更新日期：1988-06-01 00:00:00

abstract：:The polyamines, putrescine, spermidine, and spermine, are essential polycations, intimately involved in the regulation of cellular proliferation. Although polyamines exert dynamic effects on the conformation of nucleic acids and macromolecular synthesis in vitro, their specific functions in vivo are poorly understood....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mandal S,Mandal A,Johansson HE,Orjalo AV,Park MH

更新日期：2013-02-05 00:00:00

abstract：:Early events in the action of 17-beta-estradiol can be studied in soluble extracts of rat uterus by exposure of the estradiol-receptor protein to a DNA-cellulose matrix. After complexing with [(3)H]estradiol, the 4S receptor protein binds to the DNA, and it can be eluted with buffer of high ionic strength as a more ti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamamoto KR,Alberts BM

更新日期：1972-08-01 00:00:00

abstract：:Cyclooxygenase-2 (COX-2) expression is induced by mitogenic and proinflammatory factors. Its overexpression plays a causal role in inflammation and tumorigenesis. COX-2 expression is tightly regulated, but the mechanisms are largely unclear. Here we show the control of COX-2 expression by an endogenous tryptophan meta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng HH,Kuo CC,Yan JL,Chen HL,Lin WC,Wang KH,Tsai KK,Guvén H,Flaberg E,Szekely L,Klein G,Wu KK

更新日期：2012-08-14 00:00:00

abstract：:Unlike the dominant role of one class II invariant chain peptide (CLIP) in blocking MHC class II, comparative lipidomics analysis shows that human cluster of differentiation (CD) proteins CD1a, CD1b, CD1c, and CD1d bind lipids corresponding to hundreds of diverse accurate mass retention time values. Although most ions...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang S,Cheng TY,Young DC,Layre E,Madigan CA,Shires J,Cerundolo V,Altman JD,Moody DB

更新日期：2011-11-29 00:00:00

abstract：:Although major research efforts have focused on how specific components of foodstuffs affect health, relatively little is known about a more fundamental aspect of diet, the frequency and circadian timing of meals, and potential benefits of intermittent periods with no or very low energy intakes. The most common eating...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mattson MP,Allison DB,Fontana L,Harvie M,Longo VD,Malaisse WJ,Mosley M,Notterpek L,Ravussin E,Scheer FA,Seyfried TN,Varady KA,Panda S

更新日期：2014-11-25 00:00:00

abstract：:The different isoforms of PDGF bind with different affinities to two different receptor types. Previously, these receptors have been demonstrated mainly on mesenchymal and glial cells. We show here that PDGF beta-type receptors are present also on rat brain neurons in vivo as well as in vitro. Immunohistochemical anal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smits A,Kato M,Westermark B,Nistér M,Heldin CH,Funa K

更新日期：1991-09-15 00:00:00

abstract：:We demonstrate how a conventional confocal spinning-disk (CSD) microscope can be converted into a doubly resolving image scanning microscopy (ISM) system without changing any part of its optical or mechanical elements. Making use of the intrinsic properties of a CSD microscope, we illuminate stroboscopically, generati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz O,Pieper C,Clever M,Pfaff J,Ruhlandt A,Kehlenbach RH,Wouters FS,Großhans J,Bunt G,Enderlein J

更新日期：2013-12-24 00:00:00

abstract：:Glutamate transporters are essential for recovery of the neurotransmitter glutamate from the synaptic cleft. Crystal structures in the outward- and inward-facing conformations of a glutamate transporter homolog from archaebacterium Pyrococcus horikoshii, sodium/aspartate symporter GltPh, suggested the molecular basis ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruan Y,Miyagi A,Wang X,Chami M,Boudker O,Scheuring S

更新日期：2017-02-14 00:00:00

abstract：:The Late Quaternary glacial-interglacial transitions represent the highest amplitude climate changes over the last million years. Unraveling the sequence of events and feedbacks at Termination III (T-III), including potential abrupt climate reversals similar to those of the last Termination, has been particularly chal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pérez-Mejías C,Moreno A,Sancho C,Bartolomé M,Stoll H,Cacho I,Cheng H,Edwards RL

更新日期：2017-09-19 00:00:00