Abstract:
OBJECTIVE:To evaluate the effect of hypoxia-inducible factor-1alpha (HIF-1alpha) over-expression on the invasion-associated proteins in human prostate cancer cells, as HIF-1alpha is a transcriptional factor that could activate genes involved in the response to hypoxia, but might also enhance the invasive potency of prostate cancer cells. MATERIALS AND METHODS:Prostate cancer (LNCaP) cells were transfected with recombinant plasmid pcDNA3.1(-)/HIF-1alpha and pcDNA3.1(-) control vector using a commercial system, designated as LNCaP/HIF-1alpha and LNCaP/pcDNA3.1, respectively. The positive clones were selected with G418 and confirmed by Western blot and indirect immunofluorescence labelling. A polycarbonate filter, coated with a matrix gel, was used to analyse the invasive potency. The expression of E-cadherin, vimentin, cathepsin D, matrix metalloproteinase (MMP2) and urokinase plasminogen activator receptor (uPAR) was detected by Western blot. RESULTS:The expression level of HIF-1alpha in LNCaP/HIF-1alpha was distinctly higher than that in LNCaP/pcDNA3.1 and LNCaP. Many more cells of LNCaP/HIF-1alpha penetrated through the polycarbonate filter than those of LNCaP/pcDNA3.1 and LNCaP. Compared with the LNCaP/pcDNA3.1 and LNCaP cells, the expression of vimentin, cathepsin D, MMP-2 and uPAR were up-regulated in LNCaP/HIF-1alpha, whereas the expression of E-cadherin was down-regulated. CONCLUSION:These results show that over-expression of HIF-1alpha directly stimulates the invasive potency of human prostate carcinoma LNCaP cells through the matrix gel. The expression of E-cadherin, vimentin, cathepsin D, MMP-2 and uPAR, which are proteins with established roles in the pathophysiology of invasion, could be regulated by HIF-1alpha in human prostate cancer cells.
journal_name
BJU Intjournal_title
BJU internationalauthors
Luo Y,He DL,Ning L,Shen SL,Li L,Li X,Zhau HE,Chung LWdoi
10.1111/j.1464-410X.2006.06480.xsubject
Has Abstractpub_date
2006-12-01 00:00:00pages
1315-9issue
6eissn
1464-4096issn
1464-410Xpii
BJU6480journal_volume
98pub_type
杂志文章abstract:OBJECTIVE:To assess the possible relationship between treatment with selective serotonin-reuptake inhibitors (SSRIs) and the occurrence of nocturia. SUBJECTS AND METHODS:An unselected group of adult men and women, living in the city of Ostersund, Sweden, were sent a postal questionnaire containing questions on somatic...
journal_title:BJU international
pub_type: 杂志文章
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journal_title:BJU international
pub_type: 杂志文章,评审
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更新日期:2011-10-01 00:00:00
abstract:OBJECTIVE:To assess the efficacy and safety of a transportable extracorporeal shock wave lithotripsy (ESWL) machine, the Modulith SLX-F2(TM) (Storz Medical Italia, Rome, Italy), in the management of solitary urinary calculi. PATIENTS AND METHODS:The study included 233 patients (mean age 51 years; 172 male, 61 female) ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2007.07039.x
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pub_type: 杂志文章,随机对照试验
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更新日期:2012-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1464-410x.2000.00484.x
更新日期:2000-03-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2012-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-03-01 00:00:00
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pub_type: 杂志文章,多中心研究
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pub_type: 临床试验,杂志文章,随机对照试验
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pub_type: 杂志文章,多中心研究
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更新日期:1999-03-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.1999.00019.x
更新日期:1999-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1464-410X.2005.05851.x
更新日期:2005-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1464-410X.2011.10164.x
更新日期:2011-12-01 00:00:00
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更新日期:2014-07-01 00:00:00
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更新日期:2015-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1464-410X.2006.06726.x
更新日期:2007-04-01 00:00:00
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