Abstract:
:The spatial specificity and stability of subplasmalemmal domains of high-polarized mitochondria (Delta Psi m(HIGH)) in human oocytes and cleavage stage embryos were investigated in instances where changes in pericortical/subplasmalemmal organization resulted in the corresponding cytoplasm becoming translucent and largely devoid of mitochondria, either by experimental manipulation or as a result of spontaneous, stage-specific morphodynamic processes. The developmental significance of high-polarized mitochondria was examined in early embryos where their loss from a cell(s) occurred by elimination into a focal cluster of small extracellular fragments, or by disproportionate segregation between blastomeres. The findings demonstrate that pronounced changes in the organization of the pericortical and subplasmalemmal cytoplasm do not affect these domains, and their displacement under experimental conditions is fully reversible. By contrast, in instances of their significant or complete elimination, the domains of high-polarized mitochondria did not reform and the affected cell(s) remained intact and undivided during culture. The findings (i) support a spatial specificity and stability for high-polarized mitochondria, which in human oocytes and early embryos, represent a very small fraction of the total mitochondrial complement, and (ii) are discussed with respect to their suggested participation in transmembrane and signal transduction processes. It is proposed that Delta Psi(HIGH) mitochondria located in subplasmalemmal domains are 'vanguard' organelles that can sense and respond to regulatory signals of extrinsic and intrinsic origin during early development.
journal_name
Reprod Biomed Onlinejournal_title
Reproductive biomedicine onlineauthors
Van Blerkom J,Davis Pdoi
10.1016/s1472-6483(10)60622-0subject
Has Abstractpub_date
2006-08-01 00:00:00pages
246-54issue
2eissn
1472-6483issn
1472-6491pii
S1472-6483(10)60622-0journal_volume
13pub_type
杂志文章abstract::Time-lapse technique provides opportunities to observe the dynamic process of human early development. Previous studies have suggested several abnormal division patterns were associated with decreased developmental potential, but no systematic results are currently available. In this study, seven abnormal division pat...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2015.02.008
更新日期:2015-06-01 00:00:00
abstract::The bi-parental genetic state is not a given after assisted reproduction. This is based on a biological definition of parentage that concerns generational inheritance of genetic material. Often three or more individuals may participate in artificial reproduction. Only cytoplasmic and spindle transfer can result in the...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2013.03.008
更新日期:2013-06-01 00:00:00
abstract::The dramatic increase in the incidence of high order multiple pregnancies that has occurred since the mid-1970s is due to three main factors: increasing female age at conception; increasing use of ovulation induction agents and the introduction of sophisticated assisted reproduction techniques. High order multiple pre...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)61854-8
更新日期:2003-04-01 00:00:00
abstract::This proceedings report presents the outcomes from an international Expert Meeting to establish a consensus on the recommended technical and operational requirements for air quality within modern assisted reproduction technology (ART) laboratories. Topics considered included design and construction of the facility, as...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2018.02.005
更新日期:2018-06-01 00:00:00
abstract::The aim of this study was, firstly, to define the different patterns of early cleavage (EC) observed at 26 h after either IVF or intracytoplasmic sperm injection (ICSI) and, secondly, to assess the predictive value of one of these patterns, even EC (EEC), on pregnancy rate in combination with day 2 embryo score. In th...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)60870-x
更新日期:2007-03-01 00:00:00
abstract::Serum anti-Müllerian hormone (AMH) concentrations decline with increasing age and constitute a sensitive marker for ovarian ageing. In addition, basal serum AMH concentrations predict ovarian response during IVF cycles. Concomitantly, oocyte quantity and embryo quality decrease with advancing age. Hence, it was postul...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)60480-4
更新日期:2008-05-01 00:00:00
abstract::In male infertility, complementary treatment with antioxidant-containing food supplements quadruples the spontaneous pregnancy rate and reduces the cost per pregnancy by 60%. ...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2011.05.009
更新日期:2011-09-01 00:00:00
abstract::This study reports two clinical pregnancies and one live birth following the transfer of vitrified blastocysts developed from oocytes with neither zona pellucida nor corona cells. Two zona-free oocytes obtained from two patients of advanced maternal age undergoing minimal stimulation were normally fertilized after int...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2010.04.033
更新日期:2010-10-01 00:00:00
abstract::The current systematic review and meta-analysis evaluate the perinatal outcomes in twin pregnancies following multifetal pregnancy reduction (MPR) compared with non-reduced triplet pregnancies. All studies comparing perinatal outcome of twin pregnancies following MPR to non-reduced triplet pregnancies were considered....
journal_title:Reproductive biomedicine online
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.rbmo.2017.05.012
更新日期:2017-09-01 00:00:00
abstract::Endometriosis is a complex disease that is influenced by genetic and environmental factors. In endometriosis, WNT4 plays a likely role owing to its biological functions. In this study, the TaqMan allelic discrimination technique was used to investigate the relationship between endometriosis and four single nucleotide ...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2014.12.010
更新日期:2015-04-01 00:00:00
abstract::Recurrent pregnancy loss (RPL) has been associated with low expression of apoptosis-and angiogenesis-related genes. The p53 tumour suppressor gene has been shown to induce apoptosis and angiogenesis. Recently, a low increased frequency of a p53 codon 72 polymorphism has been reported among women experiencing RPL. This...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)61013-9
更新日期:2006-03-01 00:00:00
abstract::Oocyte cryopreservation has long been the focus of unsuccessful efforts to perfect its clinical application. Lately, vitrification has been shown to provide high degrees of success by different research groups. This review attempts to address the efficiency of oocyte vitrification in clinical practice through an analy...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::X-linked genetic diseases include a wide range of disorders such as the dystrophinopathies. Additionally in some rare genetic diseases, severity of expression is gender dependent. Prevention of such disorders usually involves prenatal diagnosis and termination of affected pregnancies, while preimplantation genetic dia...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)60178-2
更新日期:2009-09-01 00:00:00
abstract::Multifetal pregnancy reduction (MPR) is an accepted method of reducing complications of triplet pregnancies and higher-order multifetal pregnancies. Eighty-three pregnancies that underwent early (68 weeks) transvaginal MPR were compared with 125 pregnancies that underwent late (11-14 weeks) transabdominal MPR. Rates o...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2016.08.015
更新日期:2016-11-01 00:00:00
abstract::The non-classical major histocompatibility complex (MHC) class Ib antigens, termed HLA-G and HLA-E, have been associated with fetal maternal tolerance. The role of HLA-G in the preimplantation embryo remains unclear although immunoprotection, adhesion and cell signalling mechanisms have been suggested. Unlike HLA-G, H...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2009.11.008
更新日期:2010-02-01 00:00:00
abstract:RESEARCH QUESTION:The prevalence of infertility in couples actively trying to conceive is 25%. What is the consultation-seeking behaviour, diagnosis and related treatment in infertile couples across China? DESIGN:Large cross-sectional population-based study in 2010-2011, in which 25,270 couples from eight provinces/mu...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2018.11.034
更新日期:2019-06-01 00:00:00
abstract:RESEARCH QUESTION:What are the metabolic characteristics of homocysteine in polycystic ovary syndrome (PCOS)? DESIGN:Homocysteine concentrations were determined in serum samples from non-obese and obese control subjects and PCOS patients. Homocysteine metabolism was studied in a rat model of PCOS established using deh...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2018.05.008
更新日期:2018-08-01 00:00:00
abstract::In medicine the changes in therapies are the evidence of the progress in knowledge. But many examples concerning the treatment of early spontaneous abortion, late abortion, menopause, contraception and infertility show that gynaecologists and obstetricians change their treatments just as fashion designers change their...
journal_title:Reproductive biomedicine online
pub_type: 历史文章,杂志文章
doi:10.1016/s1472-6483(10)60903-0
更新日期:2007-04-01 00:00:00
abstract::This worldwide web-based survey compared the clinical practice for luteal-phase supplementation (LPS) in stimulated IVF cycles to the current evidence-based literature. Eighty-four treatment centres in 35 countries, representing a total of 51,155 IVF cycles/year, responded. Vaginal progesterone alone was used for LPS ...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2012.04.005
更新日期:2012-08-01 00:00:00
abstract::The maturation medium for in-vitro oocyte maturation is usually supplemented with serum. However, supplementation with serum from pregnant women adversely affects the outcome of in-vitro maturation. The purpose of the study was to assess if growth factors or granulosa cell co-culture could overcome the adverse effects...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)60068-5
更新日期:2009-08-01 00:00:00
abstract::The aim of this study was to compare the clinical performance of Wallace and Labotect embryo transfer catheters in a single assisted reproduction centre. A total of 260 women undergoing embryo transfer were randomized between Wallace and Labotect catheters. When both catheters failed to negotiate the cervical canal, t...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章,随机对照试验
doi:10.1016/s1472-6483(10)60895-4
更新日期:2007-04-01 00:00:00
abstract::More than 17,000 intrauterine insemination (lUI) cycles were analysed retrospectively with respect to outcome according to differing aetiologies of infertility. The quantity and motility of spermatozoa in the final preparation used for insemination had a positive effect on the outcome, as classically observed in the p...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)60223-4
更新日期:2008-09-01 00:00:00
abstract::The 20th century witnessed the steady development of knowledge about reproductive physiology and endocrinology in animals and humans. These advances led to the identification of higher centres governing the ovary and its follicles. Effects of X-rays on the head and other agents, or excision of the pituitary gland, led...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
doi:10.1016/s1472-6483(12)60006-6
更新日期:2002-01-01 00:00:00
abstract::The transvaginal approach to tubo-ovarian surgery allows easy and direct access to the tubo-ovarian structures and the fossa ovarica without additional manipulation. In the absence of a panoramic view, the proximity of the tubo-ovarian structures allows operative procedures for treatment of superficial and cystic ovar...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
doi:10.1016/s1472-6483(12)60121-7
更新日期:2002-01-01 00:00:00
abstract::This paper reports on and discusses the findings of an online survey initiated by the Donor Sibling Registry of 108 parents of children conceived following oocyte donation. Respondents generally supported early disclosure of donor conception to the child, although some bias in favour of disclosure cannot be excluded, ...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2012.10.013
更新日期:2013-02-01 00:00:00
abstract::The possible relationship between endogenous LH concentrations and clinical outcome was evaluated in 750 patients treated with a standardized gonadotrophin-releasing hormone (GnRH) antagonist and recombinant FSH (rFSH)-only protocol. Serum LH concentrations were measured during stimulation by a central laboratory and ...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.rbmo.2009.12.019
更新日期:2010-04-01 00:00:00
abstract:RESEARCH QUESTION:What are the optimal values of maternal age and body mass index (BMI), paternal age and BMI, number of oocytes retrieved, and serum AMH concentrations for cumulative live birth rate (CLBR) in IVF and embryo transfer (IVF-ET)? DESIGN:This retrospective cohort study included 9494 women who underwent th...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2020.01.024
更新日期:2020-05-01 00:00:00
abstract::High daily doses of gonadotrophin-releasing hormone (GnRH) antagonists during the follicular phase of ovarian stimulation were associated with low implantation rates. To test if this occurred because of profound pituitary suppression, the pituitary response was suppressed with a high-dose GnRH antagonist and recombina...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/S1472-6483(11)60009-6
更新日期:2011-02-01 00:00:00
abstract::This prospective, observational study investigated the efficacy and tolerability of follitropin-alfa for ovarian stimulation in routine clinical practice. The study involved a large, unselected population of women. Stimulation parameters and treatment outcomes were documented for 767 patients who underwent a total of ...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)61975-x
更新日期:2001-01-01 00:00:00
abstract::This study investigated the effects of long-term (24h) in-vitro sperm incubation at room temperature (RT; 23°C) versus testis temperature (35°C) on various sperm-quality parameters. Semen samples (n=41) were prepared both by density-gradient centrifugation (DGC) and the swim-up technique in order to compare the influe...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2013.12.005
更新日期:2014-04-01 00:00:00