C-terminal truncation of the retinoblastoma gene product leads to functional inactivation.

abstract：:GM54VA human cells transformed by simian virus 40 (SV40) were fused with peritoneal macrophages obtained from three different mouse strains. All 27 hybrid clones studied were positive for SV40 tumor antigen in 100% of their cells and contained human chromosome 17. Human chromosome 17 was the only human chromosome pres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Croce CM

更新日期：1977-01-01 00:00:00

abstract：:Whole-cell voltage clamp was used to investigate the properties and spatial distribution of fast-deactivating (FD) Ca channels in squid giant fiber lobe (GFL) neurons. Squid FD Ca channels are reversibly blocked by the spider toxin omega-Agatoxin IVA with an IC50 of 240-420 nM with no effect on the kinetics of Ca chan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McFarlane MB,Gilly WF

更新日期：1996-05-14 00:00:00

abstract：:N6-Cyclohexyl[3H]adenosine ([3H]CHA) and 1,3-diethyl-8-[3H]phenylxanthine ([3H]DPX) to bind to adenosine receptors in brain membranes. The agonist [3H]CHA has high affinity in both bovine and guinea pig brain (Kd, 0.7 nM and 6 nM, respectively). [3H]CHA binding kinetics are slow (dissociation t1/2;60 min); binding is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bruns RF,Daly JW,Snyder SH

更新日期：1980-09-01 00:00:00

abstract：:Glyoxalase I forms part of the glyoxalase pathway that detoxifies reactive aldehydes such as methylglyoxal, using the spontaneously formed glutathione hemithioacetal as substrate. All known eukaryotic enzymes contain zinc as their metal cofactor, whereas the Escherichia coli glyoxalase I contains nickel. Database mini...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vickers TJ,Greig N,Fairlamb AH

更新日期：2004-09-07 00:00:00

abstract：:Myocytes in the atria contain a prohormone that gives rise to atrial natriuretic peptides (ANP), which have intrinsic hemodynamic regulatory activity. Little is known about mechanisms regulating ANP release. In rats with indwelling catheters, acute blood volume expansion with 5% (wt/vol) dextrose increases the amount ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zamir N,Haass M,Dave JR,Zukowska-Grojec Z

更新日期：1987-01-01 00:00:00

abstract：:Copper is a required metal nutrient for life, but global or local alterations in its homeostasis are linked to diseases spanning genetic and metabolic disorders to cancer and neurodegeneration. Technologies that enable longitudinal in vivo monitoring of dynamic copper pools can help meet the need to study the complex ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heffern MC,Park HM,Au-Yeung HY,Van de Bittner GC,Ackerman CM,Stahl A,Chang CJ

更新日期：2016-12-13 00:00:00

abstract：:Insufficient and disrupted sleep is linked with cardiovascular and metabolic dysregulation and morbidity. The current study examines the degree to which differences in sleep between black/African American (AA) and white/European American (EA) adults explain racial differences in cardiometabolic (CMB) disease risk. Tot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curtis DS,Fuller-Rowell TE,El-Sheikh M,Carnethon MR,Ryff CD

更新日期：2017-08-15 00:00:00

abstract：:We investigate the hypothesis that two classes of Ca2+ currents, one quickly inactivated by Ca2+ and one slowly inactivated by voltage, contribute to bursting electrical activity in pancreatic islets. A mathematical model of these currents is fit to the experimental whole-cell current-voltage and inactivation profiles...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keizer J,Smolen P

更新日期：1991-05-01 00:00:00

abstract：:We show here that a cell-wall invertase encoded by the Incw1 gene is regulated at both the transcriptional and posttranscriptional levels by sugars in a heterotrophic cell suspension culture of maize. The Incw1 gene encoded two transcripts: Incw1-S (small) and Incw1-L (large); the size variation was attributable to di...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng WH,Taliercio EW,Chourey PS

更新日期：1999-08-31 00:00:00

abstract：:Components in cerebrospinal fluid that are antigenically related to myelin basic protein have been identified by a technique described recently [Barbarese, E., Braun, P. E. & Carson, J. H. (1977) Proc. Natl. Acad. Sci. USA 74, 3360-3364] involving separating the cerebrospinal fluid proteins by sodium dodecyl sulfate/p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carson JH,Barbarese E,Braun PE,McPherson TA

更新日期：1978-04-01 00:00:00

abstract：:Down syndrome (DS) caused by trisomy of chromosome 21 is the most common genetic cause of intellectual disability. Although the prenatal diagnosis of DS has become feasible, there are no therapies available for the rescue of DS-related neurocognitive impairment. A growth inducer newly identified in our screen of neura...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakano-Kobayashi A,Awaya T,Kii I,Sumida Y,Okuno Y,Yoshida S,Sumida T,Inoue H,Hosoya T,Hagiwara M

更新日期：2017-09-19 00:00:00

abstract：:The lac operon of Escherichia coli spans approximately 5300 base pairs and includes the lacZ, lacY, and lacA genes in addition to the operator, promoter, and transcription termination regions. We report here the sequence of the lacA gene and the region distal to it, confirming the sequence of thiogalactoside transacet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hediger MA,Johnson DF,Nierlich DP,Zabin I

更新日期：1985-10-01 00:00:00

abstract：:While the physical dimensions of climate change are now routinely assessed through multimodel intercomparisons, projected impacts on the global ocean ecosystem generally rely on individual models with a specific set of assumptions. To address these single-model limitations, we present standardized ensemble projections...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lotze HK,Tittensor DP,Bryndum-Buchholz A,Eddy TD,Cheung WWL,Galbraith ED,Barange M,Barrier N,Bianchi D,Blanchard JL,Bopp L,Büchner M,Bulman CM,Carozza DA,Christensen V,Coll M,Dunne JP,Fulton EA,Jennings S,Jones MC,

更新日期：2019-06-25 00:00:00

abstract：:We introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridizat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dirks RM,Pierce NA

更新日期：2004-10-26 00:00:00

abstract：:The apoproteins of pulmonary surfactant (PSAP) are thought to be critical for normal surfactant function. They bind to surfactant phospholipids and enhance their ability to form surface films in vitro. These acidic glycoproteins have monomeric molecular weights of 36,000, 32,000, and 28,000 (PSAP-36, -32, and -28). Ea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benson B,Hawgood S,Schilling J,Clements J,Damm D,Cordell B,White RT

更新日期：1985-10-01 00:00:00

abstract：:Cytochrome c oxidase (ferrocytochrome c: oxygen oxidoreductase, EC 1.9.3.1) has been resolved into a pair of complexes of unequal molecular weight. The larger complex (electron transfer complex) contains exclusively the oxidation-reduction proteins characteristic of cytochrome oxidase; the smaller complex (ion transfe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fry M,Vande Zande H,Green DE

更新日期：1978-12-01 00:00:00

abstract：:E2F is a cellular transcription factor that binds to two sites in the adenovirus E2 promoter. Previous experiments have implicated E2F in the E1A-dependent transactivation of the E2 gene since levels of active E2F increase markedly during adenovirus infection in parallel with the increase in E2 transcription, and an E...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hiebert SW,Lipp M,Nevins JR

更新日期：1989-05-01 00:00:00

abstract：:Cell-free extracts of Arbacia eggs (Arbacia punctulata) apparently do not contain an enzymatic system for the reduction of ribonucleotides to deoxyribonucleotides. However, during an interval of 5 hr after fertilization at 23 degrees , an enzymatic system is produced that is capable of catalyzing the reduction of CDP ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noronha JM,Sheys GH,Buchanan JM

更新日期：1972-08-01 00:00:00

abstract：:A mutation that confers resistance to the drug paromomycin is shown to be in the structural gene that codes for the ribosomal RNA in Tetrahymena. This observation was made by exploiting a variant of the ribosomal DNA that distorts amplification of this locus when a new somatic nucleus develops during conjugation. Beca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bruns PJ,Katzen AL,Martin L,Blackburn EH

更新日期：1985-05-01 00:00:00

abstract：:A human peripheral T-cell cDNA library was screened with two labeled synthetic oligonucleotides encoding regions of a human placenta protein-tyrosine-phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48). One positive clone was isolated and the nucleotide sequence was determined. It contained 1305 bas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cool DE,Tonks NK,Charbonneau H,Walsh KA,Fischer EH,Krebs EG

更新日期：1989-07-01 00:00:00

abstract：:Reaction of the normal isomer of [B20H18]2- and the protected thiol anion, [SC(O)OC(CH3)3]-, produces an unexpected isomer of [B20H17SC(O)OC(CH3)3]4- directly and in good yield. The isomer produced under mild conditions is characterized by an apical-apical boron atom intercage connection as well as the location of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feakes DA,Waller RC,Hathaway DK,Morton VS

更新日期：1999-05-25 00:00:00

abstract：:ExoU is a type III-secreted cytotoxin expressing A2 phospholipase activity when injected into eukaryotic target cells by the bacterium Pseudomonas aeruginosa The enzymatic activity of ExoU is undetectable in vitro unless ubiquitin, a required cofactor, is added to the reaction. The role of ubiquitin in facilitating Ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tessmer MH,Anderson DM,Pickrum AM,Riegert MO,Moretti R,Meiler J,Feix JB,Frank DW

更新日期：2018-01-16 00:00:00

abstract：:A single-nucleotide polymorphism in the human arylamine N-acetyltransferase 2 (Nat2) gene has recently been identified as associated with insulin resistance in humans. To understand the cellular and molecular mechanisms by which alterations in Nat2 activity might cause insulin resistance, we examined murine ortholog N...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Camporez JP,Wang Y,Faarkrog K,Chukijrungroat N,Petersen KF,Shulman GI

更新日期：2017-12-26 00:00:00

abstract：:Reef-building corals are critically important species that are threatened by anthropogenic stresses including climate change. In attempts to understand corals' responses to stress and other aspects of their biology, numerous genomic and transcriptomic studies have been performed, generating a variety of hypotheses abo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cleves PA,Strader ME,Bay LK,Pringle JR,Matz MV

更新日期：2018-05-15 00:00:00

abstract：:Widespread belief in misinformation circulating online is a critical challenge for modern societies. While research to date has focused on psychological and political antecedents to this phenomenon, few studies have explored the role of digital media literacy shortfalls. Using data from preregistered survey experiment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guess AM,Lerner M,Lyons B,Montgomery JM,Nyhan B,Reifler J,Sircar N

更新日期：2020-07-07 00:00:00

abstract：:T-box riboswitches control transcription of downstream genes through the tRNA-binding formation of terminator or antiterminator structures. Previously reported T-boxes were described as single-specificity riboswitches that can bind specific tRNA anticodons through codon-anticodon interactions with the nucleotide tripl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saad NY,Stamatopoulou V,Brayé M,Drainas D,Stathopoulos C,Becker HD

更新日期：2013-07-30 00:00:00

abstract：:We describe a synthetic ligand, "DAKLI" (Dynorphin A-analogue Kappa LIgand), related to the opioid peptide dynorphin A. A single reactive amino group at the extended carboxyl terminus permits various reporter groups to be attached, such as 125I-labeled Bolton-Hunter reagent, fluorescein isothiocyanate, or biotin. Thes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldstein A,Nestor JJ Jr,Naidu A,Newman SR

更新日期：1988-10-01 00:00:00

abstract：:Using a proteomics screen, we have identified the methyltransferase G9a as an interacting partner of the hematopoietic activator NF-E2. We show that G9a is recruited to the beta-globin locus in a NF-E2-dependent manner and spreads over the entire locus. While G9a is often regarded as a corepressor, knocking down this ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaturvedi CP,Hosey AM,Palii C,Perez-Iratxeta C,Nakatani Y,Ranish JA,Dilworth FJ,Brand M

更新日期：2009-10-27 00:00:00

abstract：:Treatment of a human breast cancer cell line (MDA-MB-435) in nude mice with a recombinant adenovirus containing the human interferon (IFN) consensus gene, IFN-con1 (ad5/IFN), resulted in tumor regression in 100% of the animals. Tumor regression occurred when virus was injected either within 24 hr of tumor cell implant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang JF,Hu C,Geng Y,Selm J,Klein SB,Orazi A,Taylor MW

更新日期：1996-04-30 00:00:00

abstract：:The myocardin family proteins (myocardin, MRTF-A, and MRTF-B) are serum response factor (SRF) cofactors and potent transcription activators. Gene-ablation studies have indicated important developmental functions for myocardin family proteins primarily in regulation of cardiac and smooth muscle development. Using Xenop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meadows SM,Warkman AS,Salanga MC,Small EM,Krieg PA

更新日期：2008-02-05 00:00:00