"DAKLI": a multipurpose ligand with high affinity and selectivity for dynorphin (kappa opioid) binding sites.

abstract：:August-Copenhagen-Irish (ACI) rats are unique in that the ovary-intact females develop high incidence of mammary cancers induced solely by hormones upon prolonged exposure to high levels of estrogen alone. Studies have also shown that such prolonged exposure to high-dose estrogen results in human-like aneuploid mammar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blank EW,Wong PY,Lakshmanaswamy R,Guzman R,Nandi S

更新日期：2008-03-04 00:00:00

abstract：:The beta plasmid from Streptococcus faecalis strain DS5, which codes for resistance to erythromycin and lincomycin, was introduced into a Lancefield group F streptococcus, strain DR1501, by transformation. This strain, DR1501 (beta), was found to be an excellent donor of the beta plasmid and readily transferred the re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： LeBlanc DJ,Hawley RJ,Lee LN,St Martin EJ

更新日期：1978-07-01 00:00:00

abstract：:General control nonderepressible 2 (GCN2) plays a major role in the cellular response to amino acid limitation. Although maintenance of amino acid homeostasis is critical for tumor growth, the contribution of GCN2 to cancer cell survival and proliferation is poorly understood. In this study, we generated GCN2 inhibito...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura A,Nambu T,Ebara S,Hasegawa Y,Toyoshima K,Tsuchiya Y,Tomita D,Fujimoto J,Kurasawa O,Takahara C,Ando A,Nishigaki R,Satomi Y,Hata A,Hara T

更新日期：2018-08-14 00:00:00

abstract：:To identify proteins that regulate the transcriptional activity of c-Jun, we have used the yeast two-hybrid screen to detect mammalian polypeptides that might interact functionally with the N-terminal segment of c-Jun, a known regulatory region. Among the proteins identified is a short form of Stat3 (designated Stat3 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schaefer TS,Sanders LK,Nathans D

更新日期：1995-09-26 00:00:00

abstract：:Phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR) by cAMP-dependent protein kinase leads to chloride flux in epithelial cells. Is CFTR also required for the calcium-dependent activation of chloride channels? We used antisense oligodeoxynucleotides to CFTR to reduce the expression of CFT...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wagner JA,McDonald TV,Nghiem PT,Lowe AW,Schulman H,Gruenert DC,Stryer L,Gardner P

更新日期：1992-08-01 00:00:00

abstract：:Neuroglobin (Ngb), a vertebrate globin expressed primarily in neurons, is induced by and protects against neuronal hypoxia and cerebral ischemia. To investigate the spectrum and mechanism of Ngb's neuroprotective action, we studied the effect of transgenic overexpression of Ngb on NMDA and beta-amyloid (Abeta) toxicit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khan AA,Mao XO,Banwait S,Jin K,Greenberg DA

更新日期：2007-11-27 00:00:00

abstract：:Thioredoxins are 12-kDa proteins functional in the regulation of cellular processes throughout the animal, plant, and microbial kingdoms. Growing evidence with seeds suggests that an h-type of thioredoxin, reduced by NADPH via NADP-thioredoxin reductase, reduces disulfide bonds of target proteins and thereby acts as a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yano H,Wong JH,Lee YM,Cho MJ,Buchanan BB

更新日期：2001-04-10 00:00:00

abstract：:Reported effects of anti-CD154 treatment on autoimmunity, alloreactivity, and inflammatory events mediated by macrophages and endothelial cells indicated that it might be an ideal agent for the prevention of intrahepatic islet allograft failure. This hypothesis was tested in MHC-mismatched rhesus monkeys. Transplantat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kenyon NS,Chatzipetrou M,Masetti M,Ranuncoli A,Oliveira M,Wagner JL,Kirk AD,Harlan DM,Burkly LC,Ricordi C

更新日期：1999-07-06 00:00:00

abstract：:Quiescent BALB/c 3T3 cells exposed briefly to a platelet-derived growth factor (PDGF) become "competent" to replicate their DNA but do not "progress" into S phase unless incubated with growth factors contained in platelet-poor plasma. Plasma from hypophysectomized rats is deficient in progression activity; it does not...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stiles CD,Capone GT,Scher CD,Antoniades HN,Van Wyk JJ,Pledger WJ

更新日期：1979-03-01 00:00:00

abstract：:Phospholamban (PLN) is an effective inhibitor of the sarco(endo)plasmic reticulum Ca(2+) ATPase (SERCA). Here, we examined PLN stability and degradation in primary cultured mouse neonatal cardiomyocytes (CMNCs) and mouse hearts using immunoblotting, molecular imaging, and [(35)S]methionine pulse-chase experiments, tog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teng AC,Miyake T,Yokoe S,Zhang L,Rezende LM Jr,Sharma P,MacLennan DH,Liu PP,Gramolini AO

更新日期：2015-06-09 00:00:00

abstract：:The ability to instantly create a state of immunity as achieved in the passive transfer of hyperimmune globulin has had a tremendous impact on public health. Unlike passive immunization, active immunization, which is the foundation of vaccinology, is an anticipatory strategy with inherent limitations. Here we show tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Popkov M,Gonzalez B,Sinha SC,Barbas CF 3rd

更新日期：2009-03-17 00:00:00

abstract：:An electron microscopic study of somatic hybrids resulting from a cross between hamster melanoma cells harboring R-type particles and mouse fibroblasts harboring intracisternal A-type and (mostly) immature C-type particles was performed. The major findings are: (a) hybrid cells that retain a large fraction of both ham...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yotsuyanagi Y,Ephrussi B

更新日期：1974-11-01 00:00:00

abstract：:Transferrin, a nonheme iron-binding protein, is polymorphic in most vertebrate species that have been examined. In pigeons, it is controlled by an autosomal gene, with two known codominant alleles, Tf(A) and Tf(B). The two alleles are found in nearly equal frequencies and the three genotypes are at Hardy-Weinberg equi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frelinger JA

更新日期：1972-02-01 00:00:00

abstract：:We postulated that a synergistic combination of two innate immune functions, pathogen surface recognition and lysis, in a protein chimera would lead to a robust class of engineered antimicrobial therapeutics for protection against pathogens. In support of our hypothesis, we have engineered such a chimera to protect ag...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dandekar AM,Gouran H,Ibáñez AM,Uratsu SL,Agüero CB,McFarland S,Borhani Y,Feldstein PA,Bruening G,Nascimento R,Goulart LR,Pardington PE,Chaudhary A,Norvell M,Civerolo E,Gupta G

更新日期：2012-03-06 00:00:00

abstract：:Natural transformation (i.e., the uptake of DNA and its stable integration in the chromosome) is a major mechanism of horizontal gene transfer in bacteria. Although the vast majority of bacterial genomes carry the genes involved in natural transformation, close relatives of naturally transformable species often appear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Durieux I,Ginevra C,Attaiech L,Picq K,Juan PA,Jarraud S,Charpentier X

更新日期：2019-09-10 00:00:00

abstract：:Antibodies to electrolectin, a lectin endogenous to embryonic skeletal muscle, have been used to study the distribution of electrolectin during myogenesis in L6 cells and rat primary muscle cultures. Antibody binding is highest to mononucleated cells and is low to myotubes in both systems. Binding is much lower to fib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Podleski TR,Greenberg I

更新日期：1980-02-01 00:00:00

abstract：:The balance between mitochondrial fusion and fission influences the reticular shape of mitochondria in yeasts. Little is known about whether mitochondria fusion occurs in plants. Plant mitochondria are usually more numerous and more grain-shaped than animal mitochondria. BLAST searches of the nuclear and mitochondrial...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arimura S,Yamamoto J,Aida GP,Nakazono M,Tsutsumi N

更新日期：2004-05-18 00:00:00

abstract：:The retrosplenial cortex (RSC) is part of a network of interconnected cortical, hippocampal, and thalamic structures harboring spatially modulated neurons. The RSC contains head direction cells and connects to the parahippocampal region and anterior thalamus. Manipulations of the RSC can affect spatial and contextual ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Czajkowski R,Jayaprakash B,Wiltgen B,Rogerson T,Guzman-Karlsson MC,Barth AL,Trachtenberg JT,Silva AJ

更新日期：2014-06-10 00:00:00

abstract：:Some taxa in the superfamily Arctoidea, such as the giant panda and the lesser panda, have presented puzzles to taxonomists. In the present study, approximately 397 bases of the cytochrome b gene, 364 bases of the 12S rRNA gene, and 74 bases of the tRNA(Thr) and tRNA(Pro) genes from the giant panda, lesser panda, kink...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang YP,Ryder OA

更新日期：1993-10-15 00:00:00

abstract：:We have analyzed ligand-receptor complexes resulting from (i) the incubation of canine hepatic plasma membranes with [125I]iodoglucagon and (ii) subsequent gentle solubilization of receptor-bound ligand with digitonin. The complexes (molecular weight approximately equal to 500,000) retain the radiolabeled ligand durin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mason JC,Tager HS

更新日期：1985-10-01 00:00:00

abstract：:Neurotrophins are potent survival factors for developing and injured neurons. However, they are not being used to treat neurodegenerative diseases because of difficulties in administration and numerous side effects that have been encountered in previous clinical trials. Their biological activities use Trk (tropomyosin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiese S,Jablonka S,Holtmann B,Orel N,Rajagopal R,Chao MV,Sendtner M

更新日期：2007-10-23 00:00:00

abstract：:Mast cells and nerve growth factor (NGF) have both been reported to be involved in neuroimmune interactions and tissue inflammation. In many peripheral tissues, mast cells interact with the innervating fibers. Changes in the behaviors of both of these elements occur after tissue injury/inflammation. As such conditions...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leon A,Buriani A,Dal Toso R,Fabris M,Romanello S,Aloe L,Levi-Montalcini R

更新日期：1994-04-26 00:00:00

abstract：:Purine nucleoside analogs modified by replacement of the nitrogen atom at the 3 position by a CH group give a characteristic circular dichroism curve that is not substantially modified by chemical substitution at the 8 position. Since it is rather well established that 8-substituted purine nucleosides are predominantl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles DW,Townsend LB,Redington P,Eyring H

更新日期：1976-07-01 00:00:00

abstract：:Site-directed mutagenesis of selected residues of mammalian protein phosphatase 1 (PP-1) has been carried out to further define the mechanism of catalysis, activation by divalent cations, and inhibition by toxins and inhibitory proteins. Mutation of active site residues predicted to bind metals (N124D and H248N) resul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang HB,Horiuchi A,Goldberg J,Greengard P,Nairn AC

更新日期：1997-04-15 00:00:00

abstract：:Bright, photostable, and nontoxic fluorescent contrast agents are critical for biological imaging. "Self-healing" dyes, in which triplet states are intramolecularly quenched, enable fluorescence imaging by increasing fluorophore brightness and longevity, while simultaneously reducing the generation of reactive oxygen ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pati AK,El Bakouri O,Jockusch S,Zhou Z,Altman RB,Fitzgerald GA,Asher WB,Terry DS,Borgia A,Holsey MD,Batchelder JE,Abeywickrama C,Huddle B,Rufa D,Javitch JA,Ottosson H,Blanchard SC

更新日期：2020-09-29 00:00:00

abstract：:The involvement of methylation in the chemosensory response of bacteria to many attractants has been clearly established by studies in several laboratories. It has been assumed that adaptation of Salmonella typhimurium and Escherichia coli to all attractants involves methylation of a transmembrane methyl-accepting che...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Niwano M,Taylor BL

更新日期：1982-01-01 00:00:00

abstract：:Genetic manipulation of the alpha(2A)-adrenergic receptor (alpha(2A)-AR) in mice has revealed the role of this subtype in numerous responses, including agonist-induced hypotension and sedation. Unexpectedly, alpha(2)-agonist treatment of mice heterozygous for the alpha(2A)-AR (alpha(2A)-AR(+/-)) lowers blood pressure ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tan CM,Wilson MH,MacMillan LB,Kobilka BK,Limbird LE

更新日期：2002-09-17 00:00:00

abstract：:High vein density, a distinctive trait of C4 leaves, is central to both C3-to-C4 evolution and conversion of C3 to C4-like crops. We tested the hypothesis that high vein density in C4 leaves is due to elevated auxin biosynthesis and transport in developing leaves. Up-regulation of genes in auxin biosynthesis pathways ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang CF,Yu CP,Wu YH,Lu MJ,Tu SL,Wu SH,Shiu SH,Ku MSB,Li WH

更新日期：2017-08-15 00:00:00

abstract：:When purified dimers of hemagglutinin-neuraminidase molecules released by protease digestion from three strains of human parainfluenza virus 1 were used in crystallization trials, long thin needle crystals formed. Electron microscopic analysis of these needle crystals revealed that they are composed of stacks of tripl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murti KG,Takimoto T,Laver WG,Portner A

更新日期：1993-02-15 00:00:00

abstract：:Heterochromatin protein 1 (HP1), first discovered in Drosophila melanogaster, is a highly conserved chromosomal protein implicated in both heterochromatin formation and gene silencing. We report here characterization of an HP1-interacting protein, heterochromatin protein 2 (HP2), which codistributes with HP1 in the pe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaffer CD,Stephens GE,Thompson BA,Funches L,Bernat JA,Craig CA,Elgin SC

更新日期：2002-10-29 00:00:00