Abstract：
:This study was designed to determine the effects of various environmental factors on the deoxyribonucleic acid (DNA) obtained from dental pulp. Extracted teeth were subjected to the following conditions: varying pH (3,7,10); temperature (4 degrees C, 25 degrees C, 37 degrees C, incineration); humidity (20%, 66%, 98%); various types of soil (sand, potting soil, garden soil); seawater; burying the teeth outdoors, and aging (one week to six months). In addition, teeth that had been extracted and held at room temperature for 16 and 19 years were also examined. Following isolation of DNA, the samples were analyzed on yield gels to determine the concentration and integrity of the recovered DNA. Restriction digestion with Pst I was followed by electrophoresis of the generated fragments, Southern transfer to nylon membranes, and hybridization to both human and bacterial probes. It was determined that, aside from soil, the environmental conditions examined did not affect the ability to obtain high-molecular-weight human DNA from dental pulp. Restriction fragment length polymorphic (RFLP) analysis of selected samples was performed. Dental pulp patterns were compared with bloodstain exemplars, revealing matching patterns, although an increase in band-shifting was observed with extended exposure to elevated temperatures.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Schwartz TR,Schwartz EA,Mieszerski L,McNally L,Kobilinsky Lsubject
Has Abstractpub_date
1991-07-01 00:00:00pages
979-90issue
4eissn
0022-1198issn
1556-4029journal_volume
36pub_type
杂志文章abstract：:This study uses the naturalistic decision-making (NDM) perspective to examine how Dutch forensic team leaders (i.e., the officers in charge of criminal forensic research from the crime scene until the use of laboratory assistance) make decisions in real-life settings and identifies the contextual factors that might in...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01714.x
更新日期：2011-07-01 00:00:00
abstract：:The Gustafson technique was a significant contribution to forensic identification. Unfortunately, many statistical errors were present in the published articles. It now seems clear that greater precision can be achieved through modification of the original technique. One of the authors (W. R. M.) will report soon on m...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Fatal traumatic thrombosis of the left internal carotid artery occurred in a 38-year-old man following minor blunt cervical trauma during an altercation. There was no external injury observed on the head, face, or neck. Neurologic deficit developed soon after the injury, which progressed to right hemiplegia. His condi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
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更新日期：1980-10-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01005.x
更新日期：2009-05-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
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更新日期：2012-05-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.14587
更新日期：2021-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13556
更新日期：2018-03-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13613
更新日期：2018-05-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12321
更新日期：2014-03-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14597
更新日期：2021-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/1556-4029.12795
更新日期：2015-09-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00556.x
更新日期：2007-11-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00590.x
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journal_title：Journal of forensic sciences
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更新日期：2005-05-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13741
更新日期：2018-07-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12888
更新日期：2016-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:Deoxyribonucleic acid (DNA) typing of ribosomal ribonucleic acid (rRNA) genes was performed with a polymerase chain reaction (PCR) assay for species identification. A variable region of the 28S ribosomal RNA gene was amplified with primers complementary to flanking sequences phylogenetically well conserved. The produc...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Between February 2017 and August 2018, West Virginia University conducted a reliability study to determine expert performance among forensic footwear examiners in the United States. Throughout the study's duration, 70 examiners each performed 12 comparisons and reported a total of 840 conclusions. In order to assess t...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14551
更新日期：2020-11-01 00:00:00
abstract：:As part of the Forensic Ear Identification (FearID) research project, which aims to obtain estimators for the strength of evidence of earmarks found on crime scenes, a large database of earprints (over 1200 donors) has been collected. Starting from a knowledge-based approach where experts add anatomical annotations of...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,多中心研究
doi：10.1111/j.1556-4029.2006.00253.x
更新日期：2006-11-01 00:00:00
