Abstract：
:Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide with a very high mortality. Because the success of the conventional therapies is limited, gene therapy may represent an alternative for HCC management. Our earlier study has shown that Bid plays a role in the development of HCC. The aim of our study is to evaluate the possibility of using truncated Bid (tBid) as a novel therapy for HCC treatment. Two HCC cell lines, Hep3B and PLC/PRF/5, were used in the experiment. Hep3B was a p53-resistant while PLC/PRF/5 a p53-sensitive. A recombinant adenovirus-Ad/AFPtBid, which contained a tBid gene driven by an alpha-fetoprotein (AFP) promoter, was constructed. Both Hep3B and PLC/PRF/5 cells infected with Ad/AFPtBid showed a significant decrease in cell viability. The decrease in cell viability by Ad/AFPtBid resulted from apoptosis of HCC cells, evident by enhanced activity of caspases and increased release of cytochrome c. In vivo experiment was performed by the intratumor injection of Ad/AFPtBid in nude mice inoculated with Hep3B. Ad/AFPtBid injection significantly inhibited tumor growth, and tumor tissues showed a marked increase in TUNEL-positive cells. Our experiment also demonstrated that Ad/AFPtBid only targeted AFP-producing cells but not those non-AFP producing cells. In conclusion, these results indicate that the introduction of Ad/AFPtBid can not only significantly but specifically kill HCC cells that produce AFP. The cell death induced by Ad/AFPtBid in HCC cells is via an apoptotic pathway that can be independent of p53 status.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Miao J,Chen GG,Chun SY,Yun JP,Chak EC,Ho RL,Lai PBdoi
10.1002/ijc.22040subject
Has Abstractpub_date
2006-10-15 00:00:00pages
1985-93issue
8eissn
0020-7136issn
1097-0215journal_volume
119pub_type
杂志文章abstract：:The expression of the recently described rap genes, closely related in the effector region to the ras proto-oncogenes, was examined by Northern blot analysis in 41 primary human tumors. The structural and in vitro biological properties of the rap gene products suggest their possible antagonistic action in the same eff...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910440608
更新日期：1989-12-15 00:00:00
abstract：:APC10.1 is a new intestinal cell line derived from ApcMin/+ mice that retains both the heterozygous Apc genotype and a nonactivated Wnt signaling pathway and displays an early neoplastic phenotype. Although tumorigenic both in immunodepressed and in immunocompetent syngeneic mice, it requires a high cell dose and a lo...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.11690
更新日期：2004-03-20 00:00:00
abstract：:In this review, we evaluate key molecular pathways and markers of muscle-invasive bladder cancer (MIBC). Overexpression and activation of EGFR, p63, and EMT genes are suggestive of basal MIBC subtype generally responsive to chemotherapy. Alterations in PPARγ, ERBB2/3, and FGFR3 gene products and their signaling along ...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.29918
更新日期：2016-06-01 00:00:00
abstract：:Recent genome-wide scans identified several novel breast cancer risk alleles, including variants of the FGFR2, MAP3K1 and LSP1 genes, and a study of associations between these alleles and characteristics of breast cancer patients reported a borderline significant correlation between the number of FGFR2 minor alleles a...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.23924
更新日期：2009-02-01 00:00:00
abstract：:Our group has conducted two previous studies on the association between vitamin D binding protein (DBP) and renal cell carcinoma (RCC), the most common form of kidney cancer, finding strong inverse associations. We undertook the current analysis to replicate our findings in a different study population that included w...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32758
更新日期：2020-08-01 00:00:00
abstract：:Organochlorine insecticides have been studied extensively in relation to breast cancer incidence, and results from two meta-analyses have been null for late-life residues, possibly due to measurement error. Whether these compounds influence survival remains to be fully explored. We examined associations between organo...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.29806
更新日期：2016-02-01 00:00:00
abstract：:MAGE-1 and MAGE-3 or -6 are genes encoding melanoma-rejection antigens recognized by cytotoxic T lymphocytes in an HLA-A1 restriction manner. MAGE-1 and MAGE-3 or -6 were expressed in 5/14 (36%) and 6/14 (43%) neuroblastoma (NB) cell lines, and in 20/41 (49%) and 24/41 (59%) clinical NB-related tumors, respectively. A...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(SICI)1097-0215(19961021)69:5<375::AID-IJC
更新日期：1996-10-21 00:00:00
abstract：:Cancer incidence disparities exist among specific Asian American populations. However, the existing reports exclude data from large metropoles like Chicago, Houston and New York. Moreover, incidence rates by subgroup have been underestimated due to the exclusion of Asians with unknown subgroup. Cancer incidence data f...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.29958
更新日期：2016-05-01 00:00:00
abstract：:Noninvasive biomarkers for early pancreatic ductal adenocarcinoma (PDAC) diagnosis and disease risk stratification are greatly needed. We conducted a nested case-control study within the Prospective Investigation into Cancer and Nutrition (EPIC) cohort to evaluate prediagnostic microRNAs (miRs) as biomarkers of subseq...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.30790
更新日期：2017-09-01 00:00:00
abstract：:To generate non-small cell lung cancer (NSCLC)-reactive lymphocytes, we transfected an HLA-A2-expressing human NSCLC line (1355) with the cDNA encoding the lymphocyte co-stimulatory molecule CD80. Following selection in G418, 1355.7 demonstrated stable cell-surface expression of CD80. Allogeneic mixed lymphocyte tumor...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19981209)78:6<685::aid-ijc
更新日期：1998-12-09 00:00:00
abstract：:The presence of carcinogen-DNA adducts in human tissues is evidence of exposure to carcinogens and may be an indicator of cancer risk. DNA was isolated from non-tumorous bronchial tissue of 37 cigarette smokers, 8 former smokers and 8 non-smokers and analyzed for the presence of aromatic and/or hydrophobic DNA adducts...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910460403
更新日期：1990-10-15 00:00:00
abstract：:We have previously developed 3 monoclonal anti-idiotypic antibodies (Ab2) of LOU rat origin directed against the binding site of the murine monoclonal IgM LAM8, which recognizes the small-cell lung carcinoma (SCLC)-associated sialoglycoprotein antigen sGP 90-135. The aim of this study was to compare the efficiencies o...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910500118
更新日期：1992-01-02 00:00:00
abstract：:Human lymphoblastoid interferon inhibited the growth in vitro of fibroblasts and epithelial cells from normal, hyperplastic and neoplastic human breast tissue. At an interferon concentration of 10(3) inter-national units (IU) per ml, the inhibitory effects on monolayer growth were completely reversible but the growth ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910220307
更新日期：1978-09-15 00:00:00
abstract：:Chicken embryo fibroblasts and hepatocytes were studied in the presence of catalase and superoxide dismutase in order to establish whether these enzymes had the capacity to favour cell multiplication as previously shown for in vitro tumour ascites cells (ATP C+). The results indicate that, unlike ATP C+ cells, both fi...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910400312
更新日期：1987-09-15 00:00:00
abstract：:EPLIN is frequently downregulated or lost in various cancers. The purpose of this study was to evaluate the importance of EPLIN in prostate cancer progression, with particular focus on the mechanistic implications to elucidate EPLIN's tumor suppressive function in cancer. EPLIN expression was evaluated in prostate can...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.31786
更新日期：2018-11-15 00:00:00
abstract：:BRMS1 is regarded as a metastasis suppressor gene for its ability to reduce metastatic potential of human and murine breast cancer cells as well as human melanoma cells. However, BRMS1 association to human tumor progression is not clearly understood. In the present study we analyzed BRMS1 mRNA expression in tumor prog...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22379
更新日期：2007-03-15 00:00:00
abstract：:A long-term tissue culture cell line has been derived from a human prostate adenocarcinoma metastatic to the brain. The cell line, DU 145, has been passaged 90 times in vitro over a period of 2 years. The cells are epithelial, grow in isolated islands on plastic Petri dishes, and form colonies in soft agar suspension ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910210305
更新日期：1978-03-15 00:00:00
abstract：:Multiple drug resistance (MDR) is a problem that seriously reduces the efficacy of many chemotherapy agents. One mechanism for MDR is increased acidification of endocytic vesicles and increased cytosol pH, so weak base chemotherapeutic agents, including doxorubicin, are trapped in endocytic vesicles and exhibit a drug...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22098
更新日期：2006-12-01 00:00:00
abstract：:Co-injection of fibroblasts with human epithelial breast-tumor MCF7 cells in the presence of Matrigel enhances tumor growth in nude mice. While most of the matrix metalloproteinases (MMPs) have been shown to be produced by stromal cells, tumor cells such as MCF7 cells are unable to produce MMPs. We therefore, hypothes...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19980413)76:2<267::aid-ijc
更新日期：1998-04-13 00:00:00
abstract：:Apoptosis protease-activating factor 1 (APAF-1), a transcriptional target of p53, is a cytosolic adaptor protein that links the mitochondrial apoptosis pathway to the caspase cascade. Here, we aimed to study the impact of APAF-1 expression levels on cell death induced by anticancer drugs or ionizing irradiation (IR) a...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.21535
更新日期：2006-05-01 00:00:00
abstract：:DU-PAN-2 is a high-molecular-weight glycoprotein defined by a murine monoclonal antibody (MAb) against a pancreatic ductal adenocarcinoma cell line. In order to evaluate the usefulness of this antigen as a tumor marker, we determined the serum level of the antigen by competitive inhibition radioimmunoassay in sera fro...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910370509
更新日期：1986-05-15 00:00:00
abstract：:Immunochromatographical fecal occult blood tests were shown to have higher sensitivity for detecting colorectal neoplasms than the commonly used guaiac-based test. However, positivity rates, sensitivity and specificity vary widely. We aimed to assess the reasons for this heterogeneity. Six dichotomous (qualitative) im...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.25154
更新日期：2010-10-01 00:00:00
abstract：:The relationship between chemosensitivity and p53 is currently considered from two mutually exclusive points of view: (1) wt p53 increases chemosensitivity due to apoptosis and (2) wt p53 decreases chemosensitivity due to growth arrest and DNA repair. We used p53-expressing adenovirus (Ad-p53) to directly evaluate eff...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19980316)75:6<933::aid-ijc
更新日期：1998-03-16 00:00:00
abstract：:Hormonal regulation of mouse mammary tumor virus (MMTV) synthesis was studied in the CCL-51-SF cell subline derived from the Sykes' mammary tumor cell line CCL-51 and adapted to grow in semi-synthetic in vitro conditions. The virus was quantitated by measuring the supernatant reverse transcriptase activity in exogenou...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910190215
更新日期：1977-02-15 00:00:00
abstract：:Breast cancer is the leading cancer diagnosis and second most common cause of cancer deaths in sub-Saharan Africa (SSA). Yet, there are few population-level survival data from Africa and none on the survival differences by stage at diagnosis. Here, we estimate breast cancer survival within SSA by area, stage and count...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32406
更新日期：2020-03-01 00:00:00
abstract：:To elucidate the mechanisms responsible for the development of anti-estrogen resistance, we have cloned and established 3 stable ICI-182,780-resistant sub-lines, MCF-7/182R-1, MCF-7/182R-6 and MCF-7/182R-7 from the estrogen-receptor(ER)-positive and estrogen-responsive human breast-cancer MCF-7 cell line by long-term ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19970917)72:6<1129::aid-ij
更新日期：1997-09-17 00:00:00
abstract：:Modern life involves mistimed sleeping and eating patterns that in experimental studies are associated with adverse health effects. We assessed whether timing of meals is associated with breast and prostate cancer risk taking into account lifestyle and chronotype, a characteristic correlating with preference for morni...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.31649
更新日期：2018-11-15 00:00:00
abstract：:Commercial cow milk contains considerable amounts of estrogens. Our study assessed the effect of commercial low-fat milk on the development of 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary tumors in rats. Eighty 6-week-old female Sprague-Dawley rats received a single oral dose of 5 mg DMBA. Twenty-four hours l...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.20172
更新日期：2004-07-01 00:00:00
abstract：:Cancer cells have the capacity to lyse erythrocytes by a cell-contact-requiring phenomenon. Subcellular fractionation procedures have revealed that the hemolytic principle resides in the cancer cell plasma membrane. In this study we report the detergent extraction of a potent hemolytic factor from the plasma membranes...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910470217
更新日期：1991-01-21 00:00:00
abstract：:Smoking is indisputably linked to lung cancer, yet only a small fraction of smokers develops this disease. Although previously tobacco-derived carcinogens and enzyme polymorphisms have been identified to increase the risk for smokers, recent epidemiological data suggest even sex-specificity as a new and additional fac...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.32716
更新日期：2020-05-01 00:00:00
