Abstract：
:GABAA-receptors were localized in explant cultures of rat cerebellum and in dissociated primary cultures of rat cerebellar granule cells and rat cerebellar astrocytes using the monoclonal antibody bd-17 directed against the beta-subunit of the GABAA/benzodiazepine/chloride channel complex. At the light microscope level specific staining of GABAA-receptors was localized in various types of neurones in explant cultures of rat cerebellum using the indirect peroxidase-antiperoxidase (PAP) technique, whereas no specific staining was found in astrocytes. At the electron microscope level labeling of GABAA-receptors was observed in the plasma membrane of both the cell bodies and processes in dissociated primary cultures of cerebellar granule cells using an indirect preembedding immunogold staining technique which in contrast to the classical PAP technique allows quantitative estimations to be performed. Quantification of the labeling intensity revealed a higher concentration of GABAA-receptors per microns plasma membrane in the cell bodies than in the processes. In discrete areas an extremely high density of the GABAA-receptors was observed. No specific labeling of GABAA-receptors was observed in dissociated primary cultures of cerebellar astrocytes.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Hansen GH,Hösli E,Belhage B,Schousboe A,Hösli Ldoi
10.1007/BF00966097subject
Has Abstractpub_date
1991-03-01 00:00:00pages
341-6issue
3eissn
0364-3190issn
1573-6903journal_volume
16pub_type
杂志文章abstract：:Selective and limited proteolysis is a key step in the post-translational modification of peptide hormone precursors. This process appears to involve a proteolytic machinery including highly specific endoproteases. Some of the enzyme systems possibly involved in the processing of pro-neuropeptides will be described an...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00966318
更新日期：1987-10-01 00:00:00
abstract：:It is well known that antioxidants containing sulfhydryl (-SH) groups are protective against the toxic effects of mercury. The current study was designed to elucidate the mechanism(s) of the cytoprotective effects of glutathione (GSH) and N-acetylcysteine (NAC) against the toxicity of inorganic mercury (HgCl(2)) in ne...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-9962-3
更新日期：2009-09-01 00:00:00
abstract：:The effects of phenylalanine (PHE) and its deaminated metabolites phenylpyruvate (PHP), phenyllactate (PHL) and phenylacetate (PHA) on sodium and potassium activated adenosinetriphosphatase (Na+,K+-ATPase) in synaptosomes from rat brain were investigated. At very low concentrations (5-10 microM). PHE, PHL and PHA inhi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965524
更新日期：1982-06-01 00:00:00
abstract：:Effects of single and repeated doses of acrylamide on fast and slow axonal transport of radio labeled proteins following the injection of L-[4,5-3H] leucine have been studied in the optic system of male Sprague-Dawley rats. A single dose of acrylamide (100 mg/kg) had no effect, but higher concentrations (200-300 mg/kg...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973750
更新日期：1990-06-01 00:00:00
abstract：:The primary auditory cortex is subject to the modulation of numerous neurotransmitters including norepinephrine (NE), which has been shown to decrease cellular excitability by yet unclear mechanisms. We investigated the possibility that NE directly affects excitatory glutamatergic synapses. We found that bath applicat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-9966-z
更新日期：2009-11-01 00:00:00
abstract：:This study evaluates whether physiological variables differentially affect the local synthesis of protein constituents of synapses in subcellular fractions containing pinched-off dendrites (synaptodendrosomes). Synaptodendrosomes were pulse-labeled in a medium containing 35S-methionine with 3 or 25 mM KCl and in the p...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02527725
更新日期：1996-06-01 00:00:00
abstract：:Endogenous and xenobiotic sulphur-containing convulsant and non-convulsant compounds containing structural moieties of, or bearing a structural resemblance to, GABA and homocysteine were tested in binding studies for their potency in displacing the GABA-mimetic [3H]muscimol from specific, high-affinity sites (Kd = 3.6...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00971006
更新日期：1987-07-01 00:00:00
abstract：:The exposure to hypobaric hypoxia increased lipid peroxidation as indicated by thiobarbituric acid-reactive substances [TBARS] in rat brain. Plasma lactate/pyruvate ratio was used as a marker of hypoxia. We compared the protective effect of alpha-tocopherol with the effect of L-carnitine or phosphocreatine. Rats pretr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020339530924
更新日期：2002-09-01 00:00:00
abstract：:Tyrosinemia type II, also known as Richner-Hanhart syndrome, is an autosomal recessive inborn error of metabolism caused by a deficiency of hepatic cytosolic tyrosine aminotransferase, and is associated with neurologic and development difficulties in numerous patients. Considering that the mechanisms underlying the ne...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1180-3
更新日期：2013-12-01 00:00:00
abstract：:We previously showed that extracellular levels of N-acetylaspartate (NAA) increase when a medium with reduced NaCl concentration is perfused through a microdialysis probe, and proposed that NAA may be released during hypoosmotic swelling. Here, we demonstrate that this effect is due to hypoosmolarity of the perfusion ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020778832745
更新日期：1998-08-01 00:00:00
abstract：:Curcumin (diferuloyl), from the Indian spice turmeric, reduces oxidative damage and induces apoptosis. Utilizing DNA microarrays, we have demonstrated that a low (5 microM) dose of curcumin added to a mixture of astrocytes and oligodendrocytes (C6 rat glioma cells) in culture for 24 and 48 h significantly modulates ge...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9608-x
更新日期：2008-09-01 00:00:00
abstract：:The abnormal function of the voltage-gated potassium channel Kv10.2 can induce epilepsy. However, the physiological function of Kv10.2 in the central nervous system remains unclear. In this study, we found that Kv10.2 knockout (KO) increased the complexity of neurons in the CA3 subarea of hippocampus. Kv10.2 KO led to...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03143-7
更新日期：2020-12-01 00:00:00
abstract：:Several epidemiological studies have shown that consumption of large quantities of vegetables especially cruciferous vegetables (Broccoli and Brussels sprouts) can protect against chronic diseases. Sulforaphane, an isothiocynate found in cruciferous vegetables has been demonstrated to have neuroprotective effects in s...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1965-2
更新日期：2016-10-01 00:00:00
abstract：:The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging com...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-009-9922-y
更新日期：2009-04-01 00:00:00
abstract：:Mammalian MTH1 protein, a MutT-related protein, catalyzes the hydrolysis of 8-oxo-7,8-dihydroguanosine triphosphate (8-oxoGTP) to monophosphate, thereby preventing incorporation of 8-oxo-7,8-dihydroguanine (8-oxoguanine) into RNA. In this study, we applied immunohistochemistry to follow the expression of MTH1 and the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0484-4
更新日期：2011-08-01 00:00:00
abstract：:It is an honour to have this opportunity write an article in recognition of the immense contributions of Bruce Ransom to the field of glial research. For me (BAM) personally there are many highlights both as a colleague and a friend that come to mind when I reflect on the many years that I have known Bruce. My own ent...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2315-8
更新日期：2017-09-01 00:00:00
abstract：:Studies on primary astrocytes cultured in vitro have shown that process formation involves changes in cytoskeletal proteins and release of tension on the substratum. Actin filament reorganization has previously been found to be the major cytoskeletal change occurring during process formation. These changes are relativ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022413718447
更新日期：1998-03-01 00:00:00
abstract：:Age related changes in brain cortex NO metabolism were investigated in mitochondria and cytosolic extracts from youth to adulthood. Decreases of 19%, 40% and 71% in NO production were observed in mitochondrial fractions from 3, 7, and 14 months old rats, respectively, as compared with 1-month-old rats. Decreased nNOS ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9570-z
更新日期：2008-07-01 00:00:00
abstract：:The significant increase of free calcium concentration ([Ca2+]i) was found in rat cerebral cortex synaptosomes and hippocampal crude synaptosomal fraction after their exposure to glutamate. But no change of [Ca2+]i was revealed in cerebellar synaptosomes, the slight increase of [Ca2+]i in striatal synaptosomes was not...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020752101736
更新日期：1999-09-01 00:00:00
abstract：:Rabbits sensitized with whole nervous tissue or myelin basic protein (MBP) plus adjuvant and developing experimental allergic encephalomyelitis (EAE) were studied for the presence of oligoclonal immunoglobulin (Ig) bands in spinal fluid and serum. Samples obtained prior to sensitization and at the time of sacrifice we...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963909
更新日期：1981-01-01 00:00:00
abstract：:Most studies of Alzheimer's disease (AD) have focused on a single precipitating alteration as the etiological event rather than global changes closely linked to aging. Recent evidence suggests that the most significant of these global changes are metabolic. Here we present data indicating that metabolic rate, nutritio...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1025678510480
更新日期：2003-10-01 00:00:00
abstract：:The release of the inhibitory neurotransmitter GABA is generally enhanced under potentially cell-damaging conditions. The properties and regulation of preloaded [3H]GABA release from mouse hippocampal slices were now studied in free radical-containing medium in a superfusion system. Free radical production was induced...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9439-1
更新日期：2008-03-01 00:00:00
abstract：:In vitro aggregation and fibrillization of synthetic amyloid beta-protein Abeta 1-40 was assessed in the conditioned media from rhabdomyosarcoma (CRL 1598, HTB 82, HTB 153, CCL 136), adenocarcinoma (CCL 218), neuroblastoma (SY5Y), and COS cells cultured in the absence and presence of 10% heat-inactivated fetal bovine ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1027379926976
更新日期：1997-02-01 00:00:00
abstract：:The consumption of flavonoid-rich foods, in particular fruits and vegetables, has been epidemiologically associated with a reduced risk of heart disease, neurodegenerative disease, cancer and other chronic diseases. Flavonoid glycosides, the main class of flavonoids, have been shown to exert CNS-mediated activities, p...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-9969-9
更新日期：2009-10-01 00:00:00
abstract：:Association of the candidate gene DLST with late-onset Alzheimer's disease (LOAD) risk has been suggested on the basis of case-control studies. This gene, located on chromosome 14q24.3, encodes a subunit of a mitochondrial component known to be defective in AD, the alpha-ketoglutarate dehydrogenase complex. Positive r...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000014833.54481.1d
更新日期：2004-03-01 00:00:00
abstract：:Ingestion of ammonium induces hyperammonemia which increases tubulin content in cerebrum but not in cerebellum. We have dissected 11 discrete areas of cerebrum and quantified the tubulin content in control and hyperammonemic rats. An heterogeneity in the induction of tubulin is shown. The areas more affected are ventr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965516
更新日期：1989-12-01 00:00:00
abstract：:Isoleucine, together with leucine and valine, constitutes the group of branched-chain amino acids (BCAAs). BCAAs are transported from the blood into the brain parenchyma, where they can serve several distinct functions. Since brain tissue is known to oxidatively metabolize BCAAs to CO(2), they are considered as fuel m...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9840-4
更新日期：2009-01-01 00:00:00
abstract：:Many studies have demonstrated that apoptosis play an important role in cerebral ischemic pathogenesis and may represent a target for treatment. Neuroprotective effect of quercetin has been shown in a variety of brain injury models including ischemia/reperfusion. It is not clear whether BDNF-TrkB-PI3K/Akt signaling pa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0871-5
更新日期：2012-12-01 00:00:00
abstract：:Under some pathological conditions in brain, a large amount of superoxide anion (O(2)(-)) is produced, causing various cellular damages. Among three isozymes of superoxide dismutase (SOD), extracellular (EC)-SOD should play a role to detoxify O(2)(-) in extracellular space; however, a little is known about EC-SOD in b...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0882-2
更新日期：2013-01-01 00:00:00
abstract：:Subacute exposure to manganese (Mn) produced Parkinson's disease-like syndrome called Manganism. Chronic onset and progression are characteristics of Manganism, therefore, this study aimed to examine Mn toxicity following chronic exposures. Male Sprague-Dawley rats were injected Mn2+ 1 and 5 mg/kg, every 10 days for 1...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03059-2
更新日期：2020-08-01 00:00:00
