ATP-dependent Ca uptake into plant membrane vesicles.

abstract：:Programmed cell death regulates a number of biological phenomena, and the apoptotic signal must itself be tightly controlled to avoid inappropriate cell death. We established a genetic screen to search for molecules that inhibit the apoptotic signal from the Fas receptor. Here we report the isolation of a gene, LFG, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Somia NV,Schmitt MJ,Vetter DE,Van Antwerp D,Heinemann SF,Verma IM

更新日期：1999-10-26 00:00:00

abstract：:The effect of phagocytosis on lectin binding to plasma membranes of polymorphonuclear leukocytes was examined. The specific activities of binding sites of concanavalin A and Ricinus communis agglutinin (defined as mug of lectin bound per 100 mug of membrane protein) were measured on isolated membranes; they decreased ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oliver JM,Ukena TE,Berlin RD

更新日期：1974-02-01 00:00:00

abstract：:We investigate yeast sex chromosome evolution by comparing genome sequences from 16 species in the family Saccharomycetaceae, including data from genera Tetrapisispora, Kazachstania, Naumovozyma, and Torulaspora. We show that although most yeast species contain a mating-type (MAT) locus and silent HML and HMR loci str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon JL,Armisén D,Proux-Wéra E,ÓhÉigeartaigh SS,Byrne KP,Wolfe KH

更新日期：2011-12-13 00:00:00

abstract：:Tail-anchored (TA) membrane proteins are involved in a variety of important cellular functions, including membrane fusion, protein translocation, and apoptosis. The ATPase Get3 (Asna1, TRC40) was identified recently as the endoplasmic reticulum targeting factor of TA proteins. Get3 consists of an ATPase and alpha-heli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bozkurt G,Stjepanovic G,Vilardi F,Amlacher S,Wild K,Bange G,Favaloro V,Rippe K,Hurt E,Dobberstein B,Sinning I

更新日期：2009-12-15 00:00:00

abstract：:In many human cancers, tumor-specific chromosomal rearrangements are known to create chimeric products with the ability to transform cells. The EWS/WT1 protein is such a fusion product, resulting from a t(11;22) chromosomal translocation in desmoplastic small round cell tumors, where 265 aa from the EWS amino terminus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim J,Lee JM,Branton PE,Pelletier J

更新日期：1999-12-07 00:00:00

abstract：:Cucumber mosaic virus (CMV) and tomato aspermy virus (TAV) belong to the Cucumovirus genus. They have a tripartite genome consisting of single-stranded RNAs, designated 1, 2, and 3. Previous studies have shown that viable pseudorecombinants could be created in vitro by reciprocal exchanges between CMV and TAV RNA 3, b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Masuta C,Ueda S,Suzuki M,Uyeda I

更新日期：1998-09-01 00:00:00

abstract：:Chromopeptides were prepared by proteolytic digestion of phytochrome (far-red absorbing form, P(fr)) and of phycocyanin. The phycocyanobilin peptide, the chromophore of which is Z,Z,Z-configurated, was modified to the Z,Z,E isomeric chromophore. It has been demonstrated earlier that the P(fr) chromopeptide and the Z,Z...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rüdiger W,Thümmler F,Cmiel E,Schneider S

更新日期：1983-10-01 00:00:00

abstract：:Tissues from rhesus monkeys were screened by PCR for the presence of sequences homologous to known adeno-associated virus (AAV) serotypes 1-6. DNA spanning entire rep-cap ORFs from two novel AAVs, called AAV7 and AAV8, were isolated. Sequence comparisons among these and previously described AAVs revealed the greatest ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao GP,Alvira MR,Wang L,Calcedo R,Johnston J,Wilson JM

更新日期：2002-09-03 00:00:00

abstract：:Uropathogenic Escherichia coli invade bladder epithelial cells (BECs) by direct entry into specialized cAMP regulated exocytic compartments. Remarkably, a significant number of these intracellular bacteria are subsequently expelled in a nonlytic and piecemeal fashion by infected BECs. Here, we report that expulsion of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Song J,Bishop BL,Li G,Grady R,Stapleton A,Abraham SN

更新日期：2009-09-01 00:00:00

abstract：:Immediate postretrieval bilateral blockade of long-acting voltage-dependent calcium channels (L-VDCCs), but not of glutamatergic NMDA receptors, in the dorsal CA1 region of the hippocampus hinders retention of long-term spatial memory in the Morris water maze. Immediate postretrieval bilateral inhibition of calcium/ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Da Silva WC,Cardoso G,Bonini JS,Benetti F,Izquierdo I

更新日期：2013-04-16 00:00:00

abstract：:Phagocytic cells are a critical line of defense against infection. The ability of a pathogen to survive and even replicate within phagocytic cells is a potent method of evading the defense mechanisms of the host. A number of pathogens survive within macrophages after phagocytosis and this contributes to their virulenc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindgren SW,Stojiljkovic I,Heffron F

更新日期：1996-04-30 00:00:00

abstract：:Murine embryonic stem (ES) cells are permanent blastocyst-derived cell lines capable of contributing to a wide variety of tissues, including the germ line, after injection into host blastocysts. Recently, we have shown that ES cells can produce all of the cells of the developing fetus after aggregation with developmen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Forrester LM,Bernstein A,Rossant J,Nagy A

更新日期：1991-09-01 00:00:00

abstract：:Angiosperm reproduction is characterized by alternate diploid sporophytic and haploid gametophytic generations. Gametogenesis shares similarities with that of animals except for the formation of the gametophyte, whereby haploid cells undergo several rounds of postmeiotic mitosis to form gametes and the accessory cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park GT,Frost JM,Park JS,Kim TH,Lee JS,Oh SA,Twell D,Brooks JS,Fischer RL,Choi Y

更新日期：2014-12-23 00:00:00

abstract：:Molecular weights of the isolated proteins from the 30S and 50S ribosomal subunits of Escherichia coli were determined by two independent methods: polyacrylamide gel electrophoresis with sodium dodecylsulphate and equilibrium sedimentation. The values for the molecular weights determined by gel electrophoresis range f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dzionara M,Kaltschmidt E,Wittmann HG

更新日期：1970-12-01 00:00:00

abstract：:Transient expression of either alpha or alpha + beta subunits of the high-conductance Ca2+-activated K+ (maxi-K) channel has been achieved in COS-1 cells. Expression has been studied using charybdotoxin (ChTX), a peptidyl inhibitor that binds in the pore on the alpha subunit. Although some properties of monoiodotyrosi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hanner M,Schmalhofer WA,Munujos P,Knaus HG,Kaczorowski GJ,Garcia ML

更新日期：1997-04-01 00:00:00

abstract：:Targeting tyrosine kinase receptors (RTKs) with specific Abs is a promising therapeutic approach for cancer treatment, although the molecular mechanism(s) responsible for the Abs' biological activity are not completely known. We targeted the transmembrane RTK for hepatocyte growth factor (HGF) with a monoclonal Ab (DN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petrelli A,Circosta P,Granziero L,Mazzone M,Pisacane A,Fenoglio S,Comoglio PM,Giordano S

更新日期：2006-03-28 00:00:00

abstract：:A cyclic AMP-binding protein (CAP protein), cyclic AMP, and RNA polymerase holoenzyme are shown to initiate lac transcription at the lac promoter. Lac repressor appears to control transcription by preventing RNA polymerase and/or CAP protein from binding to the lac promoter. Results support the idea that the lac promo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eron L,Block R

更新日期：1971-08-01 00:00:00

abstract：:Molecular beacons are DNA probes that form a stem-and-loop structure and possess an internally quenched fluorophore. When they bind to complementary nucleic acids, they undergo a conformational transition that switches on their fluorescence. These probes recognize their targets with higher specificity than probes that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonnet G,Tyagi S,Libchaber A,Kramer FR

更新日期：1999-05-25 00:00:00

abstract：:Nitrogen metabolism in bacteria and archaea is regulated by a ubiquitous class of proteins belonging to the P(II)family. P(II) proteins act as sensors of cellular nitrogen, carbon, and energy levels, and they control the activities of a wide range of target proteins by protein-protein interaction. The sensing mechanis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rajendran C,Gerhardt EC,Bjelic S,Gasperina A,Scarduelli M,Pedrosa FO,Chubatsu LS,Merrick M,Souza EM,Winkler FK,Huergo LF,Li XD

更新日期：2011-11-22 00:00:00

abstract：:Mutants of Arabidopsis that contain reduced levels of polyunsaturated fatty acids showed growth characteristics at 22 degrees C that were very similar to wild type. By contrast, at 12 degrees C, the mutants failed to undergo stem elongation during reproductive growth although they produced normal flowers and fertile s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miquel M,James D Jr,Dooner H,Browse J

更新日期：1993-07-01 00:00:00

abstract：:The bottom-up design of protein-based signaling networks is a key goal of synthetic biology; yet, it remains elusive due to our inability to tailor-make signal transducers and receptors that can be readily compiled into defined signaling networks. Here, we report a generic approach for the construction of protein-base...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stein V,Alexandrov K

更新日期：2014-11-11 00:00:00

abstract：:Cereal agriculture originated with the domestication of barley and early forms of wheat in the Fertile Crescent. There has long been speculation that barley was domesticated more than once. We use differences in haplotype frequency among geographic regions at multiple loci to infer at least two domestications of barle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrell PL,Clegg MT

更新日期：2007-02-27 00:00:00

abstract：:A typing method for bacteria was developed and applied to several species, including Escherichia coli and Actinobacillus actinomycetemcomitans. Total genomic DNA was digested with a restriction endonuclease, and fragments were enabled with [alpha-32P]dATP by using the Klenow fragment of DNA polymerase and separated by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Steenbergen TJ,Colloms SD,Hermans PW,de Graaff J,Plasterk RH

更新日期：1995-06-06 00:00:00

abstract：:The crystal structures of Escherichia coli thymidylate kinase (TmpK) in complex with P1-(5'-adenosyl)-P5-(5'-thymidyl)pentaphosphate and P1-(5'-adenosyl)P5-[5'-(3'-azido-3'-deoxythymidine)] pentaphosphate have been solved to 2.0-A and 2.2-A resolution, respectively. The overall structure of the bacterial TmpK is very ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lavie A,Ostermann N,Brundiers R,Goody RS,Reinstein J,Konrad M,Schlichting I

更新日期：1998-11-24 00:00:00

abstract：:The kinetic inhibition of the gelation of hemoglobin S is compared to the change in hemoglobin S soulbility, when the solubility is altered by carbon monoxide, pH, or urea. By means of a new technique, the delay time and the extent of gelation are measured on the same sample. They delay time, td, is found to be propor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hofrichter J,Ross PD,Eaton WA

更新日期：1976-09-01 00:00:00

abstract：:Eph receptor tyrosine kinases and their ephrin ligands regulate cell navigation during normal and oncogenic development. Signaling of Ephs is initiated in a multistep process leading to the assembly of higher-order signaling clusters that set off bidirectional signaling in interacting cells. However, the structural an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Himanen JP,Yermekbayeva L,Janes PW,Walker JR,Xu K,Atapattu L,Rajashankar KR,Mensinga A,Lackmann M,Nikolov DB,Dhe-Paganon S

更新日期：2010-06-15 00:00:00

abstract：:Glial fibrillary acidic protein (GFAP) is a member of the family of intermediate filament structural proteins and is found predominantly in astrocytes of the central nervous system (CNS). To assess the function of GFAP, we created GFAP-null mice using gene targeting in embryonic stem cells. The GFAP-null mice have nor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCall MA,Gregg RG,Behringer RR,Brenner M,Delaney CL,Galbreath EJ,Zhang CL,Pearce RA,Chiu SY,Messing A

更新日期：1996-06-25 00:00:00

abstract：:Profilin1, a ubiquitously expressed actin-binding protein, plays a critical role in cell migration through actin cytoskeletal regulation. Given the traditional view of profilin1 as a promigratory molecule, it is difficult to reconcile observations that profilin1 is down-regulated in various invasive adenocarcinomas an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bae YH,Ding Z,Das T,Wells A,Gertler F,Roy P

更新日期：2010-12-14 00:00:00

abstract：:The Hippo pathway is an evolutionarily conserved regulator of organ growth and tumorigenesis. In Drosophila, oncogenic RasV12 cooperates with loss-of-cell polarity to promote Hippo pathway-dependent tumor growth. To identify additional factors that modulate this signaling, we performed a genetic screen utilizing the D...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo X,Sun Y,Azad T,Janse van Rensburg HJ,Luo J,Yang S,Liu P,Lv Z,Zhan M,Lu L,Zhou Y,Ma X,Zhang X,Yang X,Xue L

更新日期：2020-12-01 00:00:00

abstract：:The N-terminal arginine-rich motif of phage HK022 Nun protein binds to NUT sequences in phage lambda nascent transcripts and induces transcription termination. Interactions between the Nun C terminus and RNA polymerase as well as the DNA template are required for termination. We have isolated Nun C-terminal point and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim HC,Zhou JG,Wilson HR,Mogilnitskiy G,Court DL,Gottesman ME

更新日期：2003-04-29 00:00:00