Influence of chromatin and single strand binding proteins on the activity of an archaeal MCM.

abstract：:Historically referred to as "the GTPase center", the L11 binding region (L11BR) of Escherichia coli 23 S rRNA is a highly conserved structure that has been implicated in several essential functions during protein synthesis. Here, in vivo expression of an RNA fragment containing that structure was found to affect trans...

journal_title：Journal of molecular biology

authors： Van Dyke N,Xu W,Murgola EJ

更新日期：2002-05-31 00:00:00

abstract：:We carry out a theoretical study of the vibrational and relaxation properties of naturally occurring proteins with the purpose of characterizing both the folding and equilibrium thermodynamics. By means of a suitable model, we provide a full characterization of the spectrum and eigenmodes of vibration at various tempe...

journal_title：Journal of molecular biology

authors： Micheletti C,Lattanzi G,Maritan A

更新日期：2002-08-30 00:00:00

abstract：:Clostridium botulinum C3 exoenzyme inactivates the small GTP-binding protein family Rho by ADP-ribosylating asparagine 41, which depolymerizes the actin cytoskeleton. C3 thus represents a major family of the bacterial toxins that transfer the ADP-ribose moiety of NAD to specific amino acids in acceptor proteins to mod...

journal_title：Journal of molecular biology

authors： Han S,Arvai AS,Clancy SB,Tainer JA

更新日期：2001-01-05 00:00:00

abstract：:An open reading frame optimized for expression of 6,7-dimethyl-8-ribityl-lumazine synthase of the hyperthermophilic bacterium Aquifex aeolicus in Escherichia coli was synthesized and expressed in a recombinant E. coli strain to a level of around 15 %. The recombinant protein was purified by heat-treatment and gel-filt...

journal_title：Journal of molecular biology

authors： Zhang X,Meining W,Fischer M,Bacher A,Ladenstein R

更新日期：2001-03-09 00:00:00

abstract：:Chromosome replication initiates without sequence specificity at average intervals of approximately 10 kb during the rapid cell cycles of early Xenopus embryos. If the distribution of origins were random, some inter-origin intervals would be too long to be fully replicated before the end of S phase. To investigate wha...

journal_title：Journal of molecular biology

authors： Lucas I,Chevrier-Miller M,Sogo JM,Hyrien O

更新日期：2000-02-25 00:00:00

abstract：:LIM-kinase activated by GST-Pak1 phosphorylates Acanthamoeba actophorin stoichiometrically and specifically on serine 1. The atomic structure of phosphorylated actophorin determined by X-ray crystallography is essentially identical with the structure of unphosphorylated actophorin. We compared biochemical properties o...

journal_title：Journal of molecular biology

authors： Blanchoin L,Robinson RC,Choe S,Pollard TD

更新日期：2000-01-14 00:00:00

abstract：:The time-course of synthesis of long and short (abortive) transcripts by Escherichia coli RNA polymerase was investigated under single round conditions in vitro. The synthesis of long RNA initiated at the lambda PR (with an altered leader sequence) or at the lacUV5 promoter was completed within five minutes, but abort...

journal_title：Journal of molecular biology

authors： Kubori T,Shimamoto N

更新日期：1996-03-01 00:00:00

abstract：:Nuclear protein-coding genes of euglenids (Discoba, Euglenozoa, Euglenida) contain conventional (spliceosomal) and nonconventional introns. The latter have been found only in euglenozoans. A unique feature of nonconventional introns is the ability to form a stable and slightly conserved RNA secondary structure bringin...

journal_title：Journal of molecular biology

authors： Gumińska N,Zakryś B,Milanowski R

更新日期：2021-02-05 00:00:00

abstract：:Binary complexes between messenger RNA and E. coli ribosomes were examined. A ribosome-mRNA binary complex on T4 gene 32 mRNA withstood inhibition by antibodies against ribosomal protein S1. Anti-S1 blocks ternary complex formation, as measured by "extension inhibition" or "toeprinting" analysis, only when preincubate...

journal_title：Journal of molecular biology

authors： Hartz D,McPheeters DS,Green L,Gold L

更新日期：1991-03-05 00:00:00

abstract：:In human metallothionein-2, the exchange rate constants of ten amide protons were found to range from 1.7 x 10(-4) to 1 x 10(-1) min-1 at pH 6.3 and 8 degrees C. Most of these slowly exchanging protons could be associated with hydrogen bonds in secondary structure elements of the alpha-domain. Amide proton exchange ra...

journal_title：Journal of molecular biology

authors： Messerle BA,Bos M,Schäffer A,Vasák M,Kägi JH,Wüthrich K

更新日期：1990-08-05 00:00:00

abstract：:We have been able to observe the first step in bacteriophage infection, the docking of phage lambda to its membrane receptor maltoporin, at the single-particle level. High-resolution conductance recording from a single trimeric maltoporin channel reconstituted into a planar lipid bilayer has allowed detection of the s...

journal_title：Journal of molecular biology

authors： Gurnev PA,Oppenheim AB,Winterhalter M,Bezrukov SM

更新日期：2006-06-23 00:00:00

abstract：:The papillomavirus E2 transcription and replication factors bind to the DNA consensus ACCGN(4)CGGT sequence (E2-BS), through both direct and indirect readout mechanisms. The two symmetric half-sites ACCG.CGGT are highly conserved in the genomes and are hydrogen bound with E2. Although E2 does not contact the N4 spacer...

journal_title：Journal of molecular biology

authors： Djuranovic D,Oguey C,Hartmann B

更新日期：2004-06-11 00:00:00

abstract：:Na+/H+antiportersare a category of ubiquitous transmembrane proteins with various important physiological roles in almost all living organisms ranging from bacteria to humans. However, the knowledge of novel Na+/H+antiporters remains to be broadened, and the functional roles ofoligomerization in theseantiportershave n...

journal_title：Journal of molecular biology

authors： Shao L,Xu T,Zheng X,Shao D,Zhang H,Chen H,Zhang Z,Yan M,Abdel-Motaal H,Jiang J

更新日期：2021-01-22 00:00:00

abstract：:The molten globule has been assumed to be a major intermediate state of protein folding. To extend our understanding of protein folding it is important to elucidate the thermodynamic mechanism of conformational stability of the molten globule. To clarify the role of electrostatic charge repulsion in the stability of t...

journal_title：Journal of molecular biology

authors： Goto Y,Nishikiori S

更新日期：1991-12-05 00:00:00

abstract：:The structure of basic Winged Bean Agglutinin (WBAI) with two dimeric molecules complexed with methyl-alpha-D-galactopyranoside in the asymmetric unit, has been determined by the molecular replacement method and refined with 2.5 A X-ray intensity data. The polypeptide chain of each monomer has the characteristic legum...

journal_title：Journal of molecular biology

authors： Prabu MM,Sankaranarayanan R,Puri KD,Sharma V,Surolia A,Vijayan M,Suguna K

更新日期：1998-03-06 00:00:00

abstract：:Polar residues play important roles in the association of transmembrane helices and the stabilities of membrane proteins. Although a single Ser residue in a transmembrane helix is unable to mediate a strong association of the helices, the cooperative interactions of two or more appropriately placed serine hydroxyl gro...

journal_title：Journal of molecular biology

authors： North B,Cristian L,Fu Stowell X,Lear JD,Saven JG,Degrado WF

更新日期：2006-06-16 00:00:00

abstract：:The exceptional versatility of calmodulin (CaM) three-dimensional arrangement is reflected in the growing number of structural models of CaM/protein complexes currently available in the Protein Data Bank (PDB) database, revealing a great diversity of conformations, domain organization, and structural responses to Ca(2...

journal_title：Journal of molecular biology

authors： Villarroel A,Taglialatela M,Bernardo-Seisdedos G,Alaimo A,Agirre J,Alberdi A,Gomis-Perez C,Soldovieri MV,Ambrosino P,Malo C,Areso P

更新日期：2014-07-29 00:00:00

abstract：:The RuvAB and RecG proteins of Escherichia coli promote branch migration of Holliday junction intermediates in genetic recombination. Both are structure-specific helicases that unwind and rewind DNA at the junction point. The helicase activities of these proteins were investigated using RNA:DNA hybrid molecules. RuvAB...

journal_title：Journal of molecular biology

authors： Vincent SD,Mahdi AA,Lloyd RG

更新日期：1996-12-13 00:00:00

abstract：:The coupling between the conformational properties of double-stranded DNA and its internal dynamics has been examined. The solution structures of the isomeric DNA oligomers d(GCGTACGC)(2) (UM) and d(CGCTAGCG)(2) (CTSYM) were determined with (1)H NMR spectroscopy by utilizing distance restraints from total relaxation m...

journal_title：Journal of molecular biology

authors： Isaacs RJ,Spielmann HP

更新日期：2001-03-23 00:00:00

abstract：:A molecular dynamics (MD) simulation on the contents of two asymmetric units of the crystal structure of the bacterial serine proteinase, Streptomyces griseus proteinase A (SGPA), resulted in four dihydrogen phosphate anions migrating to form a cluster in a solvent region far removed from the protein surface. In an ef...

journal_title：Journal of molecular biology

authors： Blanchard H,James MN

更新日期：1994-08-26 00:00:00

abstract：:The C terminus, also known as the hypervariable region (residues 166-189), of H-, N-, and K-Ras proteins has sequence determinants necessary for full activation of downstream effectors such as Raf kinase and PI-3 kinase as well as for the correct targeting of Ras proteins to lipid rafts and non-raft membranes. There i...

journal_title：Journal of molecular biology

authors： Thapar R,Williams JG,Campbell SL

更新日期：2004-11-05 00:00:00

abstract：:The majority of the Mycobacterium tuberculosis response to hypoxia and nitric oxide is through the DosRS (DevRS) two-component regulatory system. The N-terminal input domain of the DosS sensor contains two GAF domains. We demonstrate here that the proximal GAF domain binds haem, and identified histidine 149 of DosS as...

journal_title：Journal of molecular biology

authors： Sardiwal S,Kendall SL,Movahedzadeh F,Rison SC,Stoker NG,Djordjevic S

更新日期：2005-11-11 00:00:00

abstract：:Streptococcus mutans is the primary etiological agent of dental caries in man and other mammalian organisms. This bacterium metabolizes carbohydrates actively and thrives under anaerobic conditions by fermenting l-ascorbate (Asc) via the sga operon, which includes SgaT, PtxB, and PtxA. These three proteins are members...

journal_title：Journal of molecular biology

authors： Lei J,Li LF,Su XD

更新日期：2009-02-20 00:00:00

abstract：:To elucidate the general constraints imposed on the structure of the D and T-loops in functional tRNAs, active suppressor tRNAs were selected in vivo from a combinatorial tRNA gene library in which several nucleotide positions in these loops were randomized. Analysis of the nucleotide sequences of the selected clones ...

journal_title：Journal of molecular biology

authors： Doyon FR,Zagryadskaya EI,Chen J,Steinberg SV

更新日期：2004-10-08 00:00:00

abstract：:Alpha-tocopherol transfer protein (alpha-TTP) is a liver protein responsible for the selective retention of alpha-tocopherol from dietary vitamin E, which is a mixture of alpha, beta, gamma, and delta-tocopherols and the corresponding tocotrienols. The alpha-TTP-mediated transfer of alpha-tocopherol into nascent VLDL ...

journal_title：Journal of molecular biology

authors： Meier R,Tomizaki T,Schulze-Briese C,Baumann U,Stocker A

更新日期：2003-08-15 00:00:00

abstract：:Bacteriophage λ of Escherichia coli has two alternative life cycles after infection-host survival with lysogen formation, or host lysis and phage production. In a lysogen, CI represses the two lytic promoters, pR and pL, and activates its own transcription from the auto-regulated pRM promoter. During induction from th...

journal_title：Journal of molecular biology

authors： Lee S,Lewis DEA,Adhya S

更新日期：2018-01-05 00:00:00

abstract：:Meiotic clade AAA ATPases (ATPases associated with diverse cellular activities), which were initially grouped on the basis of phylogenetic classification of their AAA ATPase cassette, include four relatively well characterized family members, Vps4, spastin, katanin and fidgetin. These enzymes all function to disassemb...

journal_title：Journal of molecular biology

authors： Monroe N,Hill CP

更新日期：2016-05-08 00:00:00

abstract：:The three-dimensional structure of the membrane glycoprotein neuraminidase of an escape mutant of the influenza virus strain A/Tokyo/3/67 has been determined to 3 A (1 A = 0.1 nm) resolution by X-ray diffraction. The mutant virus, selected by growing the virus in the presence of a monoclonal antibody to the neuraminid...

journal_title：Journal of molecular biology

authors： Varghese JN,Webster RG,Laver WG,Colman PM

更新日期：1988-03-05 00:00:00

abstract：:We reported recently that activation of the inositol 1,4,5-triphosphate receptor (IP3R) is required for efficient HIV-1 Gag trafficking and viral particle release. IP3R activation requires phospholipase C (PLC)-catalyzed hydrolysis of PI(4,5)P(2) to IP3 and diacylglycerol. We show that Sprouty2 (Spry2), which binds PI...

journal_title：Journal of molecular biology

authors： Ehrlich LS,Medina GN,Carter CA

更新日期：2011-07-22 00:00:00

abstract：:A duck delta II crystallin mutant, where histidine 178 has been replaced by an aspartic acid residue, has been purified from a bacterial expression system and subsequently crystallized. The crystals grow as flat plates, with unit cell dimensions a = 94.1 A, b = 99.9 A, c = 108.7 A and beta = 102 degrees. The crystals ...

journal_title：Journal of molecular biology

authors： Abu-Abed M,Turner MA,Atkinson J,Dole K,Howell PL

更新日期：1994-11-11 00:00:00