Abstract:
:We report the results of a genetic screen designed to identify transcriptional coregulators of yeast heat-shock factor (HSF). This sequence-specific activator is required to stimulate both basal and induced transcription; however, the identity of factors that collaborate with HSF in governing noninduced heat-shock gene expression is unknown. In an effort to identify these factors, we isolated spontaneous extragenic suppressors of hsp82-deltaHSE1, an allele of HSP82 that bears a 32-bp deletion of its high-affinity HSF-binding site, yet retains its two low-affinity HSF sites. Nearly 200 suppressors of the null phenotype of hsp82-deltaHSE1 were isolated and characterized, and they sorted into six expression without heat-shock element (EWE) complementation groups. Strikingly, all six groups contain alleles of genes that encode subunits of Mediator. Three of the six subunits, Med7, Med10/Nut2, and Med21/Srb7, map to Mediator's middle domain; two subunits, Med14/Rgr1 and Med16/Sin4, to its tail domain; and one subunit, Med19/Rox3, to its head domain. Mutations in genes encoding these factors enhance not only the basal transcription of hsp82-deltaHSE1, but also that of wild-type heat-shock genes. In contrast to their effect on basal transcription, the more severe ewe mutations strongly reduce activated transcription, drastically diminishing the dynamic range of heat-shock gene expression. Notably, targeted deletion of other Mediator subunits, including the negative regulators Cdk8/Srb10, Med5/Nut1, and Med15/Gal11 fail to derepress hsp82-deltaHSE1. Taken together, our data suggest that the Ewe subunits constitute a distinct functional module within Mediator that modulates both basal and induced heat-shock gene transcription.
journal_name
Geneticsjournal_title
Geneticsauthors
Singh H,Erkine AM,Kremer SB,Duttweiler HM,Davis DA,Iqbal J,Gross RR,Gross DSdoi
10.1534/genetics.105.052738keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
2169-84issue
4eissn
0016-6731issn
1943-2631pii
genetics.105.052738journal_volume
172pub_type
杂志文章相关文献
GENETICS文献大全abstract::The application of quantitative genetics in plant and animal breeding has largely focused on additive models, which may also capture dominance and epistatic effects. Partitioning genetic variance into its additive and nonadditive components using pedigree-based models (P-genomic best linear unbiased predictor) (P-BLUP...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.171322
更新日期:2014-12-01 00:00:00
abstract::hunchback (hb) is a key regulatory gene in the early segmentation gene hierarchy of Drosophila. It codes for a transcription factor of the Cys2-His2 zinc finger type and shows two separate zinc finger domains in its coding region. hb forms a morphogenetic gradient in the middle of the embryo that is required for setti...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::Data are presented concerning the gene arrangements in both arms of the X-chromosome of Drosophila robusta in eight altitudinal transects. The major change appears to be the increase of gene arrangement XL-1 (and decrease in XL, sometimes also XL-2) with increasing altitude. In each transect only one combination of XL...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1978-08-01 00:00:00
abstract::Functional analyses of the Arabidopsis genome require analysis of the gametophytic generation, since approximately 10% of the genes are expressed in the male gametophyte and approximately 9% in the female gametophyte. Here we describe the genetic and molecular characterization of 67 Ds insertion lines that show reduce...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.090852
更新日期:2009-04-01 00:00:00
abstract::Dissecting the genes involved in complex traits can be confounded by multiple factors, including extensive epistatic interactions among genes, the involvement of epigenetic regulators, and the variable expressivity of traits. Although quantitative trait locus (QTL) analysis has been a powerful tool for localizing the ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.090175
更新日期:2008-12-01 00:00:00
abstract::This study attempted to assay the zygotic contribution of X chromosome genes to the genetic control of embryonic morphogenesis in Drosophila melanogaster. A systematic screen for X-linked genes which affect the morphology of the embryo was undertaken, employing the phenotype of whole mount embryos as the major screeni...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Establishing durable disease resistance in agricultural crops, where much of the plant defense is provided through effector-R gene interactions, is complicated by the ability of pathogens to overcome R gene resistance by losing the corresponding effector gene. Many proposed methods to maintain disease resistance in th...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.2.693
更新日期:2004-02-01 00:00:00
abstract::Laterality is believed to have genetic components, as has been deduced from family studies in humans and responses to artificial selection in mice, but these genetic components are unknown and the underlying physiological mechanisms are still a subject of dispute. We measured direction of laterality (preferential use ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::The sex-ratio trait described in several Drosophila species is a type of naturally occurring X-linked meiotic drive that causes males bearing a sex-ratio X chromosome to produce progenies with a large excess of females. We have previously reported the occurrence of sex-ratio X chromosomes in Drosophila simulans. In th...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::We have isolated 15 spontaneous mutants resistant to one or several antibiotics like chloramphenicol, erythromycin and spiramycin. We have shown by several criteria that all of them result from mutations localized in the mitochondrial DNA. The mutations have been mapped by allelism tests and by two- and three-factor c...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-12-01 00:00:00
abstract::HO endonuclease-induced double-strand breaks (DSBs) within a direct duplication of Escherichia coli lacZ genes are repaired either by gene conversion or by single-strand annealing (SSA), with > 80% being SSA. Previously it was demonstrated that the RAD52 gene is required for DSB-induced SSA. In the present study, the ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::The I factor (IF) is a LINE-like transposable element from Drosophila melanogaster. IF is silenced in most strains, but under special circumstances its transposition can be induced and correlates with the appearance of a syndrome of female sterility called hybrid dysgenesis. To elucidate the relationship between IF ex...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::In this article we investigate multiplicative effects between genes in relation to heterosis. The extensive literature on heterosis due to multiplicative effects between characters is reviewed, as is earlier work on the genetic description of heterosis. A two-locus diallelic model of arbitrary gene action is used to d...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::High-throughput sequencing and bioinformatic techniques have enhanced classical genetic analysis and are essential methods for geneticists. Tsukamoto and colleagues use numerous genomic and bioinformatics methods to explore the role of ribonucleoprotein complexes in regulating oocyte meiotic maturation, which is the t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301139
更新日期:2018-07-01 00:00:00
abstract::We studied the control of pharyngeal excitation in Caenorhabditis elegans. By laser ablating subsets of the pharyngeal nervous system, we found that the MC neuron type is necessary and probably sufficient for rapid pharyngeal pumping. Electropharyngeograms showed that MC transmits excitatory postsynaptic potentials, s...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::New rudimentary (r) mutants have been isolated following mutagenesis with ethyl methanesulfonate (r(LE)), ICR-170 (r(LI)) and X rays (r(LX)). From wing phenotype measurements on homoallelic females, it has been shown that the r(LE) mutant series includes several leaky alleles, as well as alleles that produce moderate ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-09-01 00:00:00
abstract::Site-specific recombinases are widely used tools for analysis of genetics, development, and cell biology, and many schemes have been devised to alter gene expression by recombinase-mediated DNA rearrangements. Because the FRT and lox target sites for the commonly used FLP and Cre recombinases are asymmetrical, and mus...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303394
更新日期:2020-08-01 00:00:00
abstract::The worldwide pattern of single nucleotide polymorphism (SNP) variation is of great interest to human geneticists, population geneticists, and evolutionists, but remains incompletely understood. We studied the pattern in noncoding regions, because they are less affected by natural selection than are coding regions. Th...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::Temporally spaced genetic data allow for more accurate inference of population genetic parameters and hypothesis testing on the recent action of natural selection. In this work, we develop a novel likelihood-based method for jointly estimating selection coefficient and allele age from time series data of allele freque...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303400
更新日期:2020-10-01 00:00:00
abstract::Genic variation in natural populations of Drosophila simulans was surveyed using allozymic and two-dimensional electrophoretic techniques. Consistent with some previous reports, allozymic heterozygosity appeared lower than in the sibling species D. melanogaster (0.07 vs. 0.16). No variation was detected by two-dimensi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Genic heterozygosity and variation were studied in the permanent translocation heterozygotes Oenothera biennis I, Oe. biennis II, Oe. biennis III, Oe. strigosa, Oe. parviflora I, Oe. parviflora II, and in the related bivalent formers Oe. argillicola and Oe. hookeri. From variation at 20 enzyme loci, we find that trans...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-03-01 00:00:00
abstract::We derive a formula for the distribution of the length T of the recombination interval containing a target gene and using N gametes in a region where R kilobases correspond to 1 cM. The formula can be used to calculate the number of meiotic events required to narrow a target gene down to a specific interval size and h...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::We compared allele sequences of two loci near the Arabidopsis lyrata self-incompatibility (S) loci with sequences of A. thaliana orthologs and found high numbers of shared polymorphisms, even excluding singletons and sites likely to be highly mutable. This suggests maintenance of entire S-haplotypes for long evolution...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.051938
更新日期:2006-04-01 00:00:00
abstract::F1 hybrids between mouse inbred strains PWD and C57BL/6 represent the most thoroughly genetically defined model of hybrid sterility in vertebrates. Hybrid male sterility can be fully reconstituted from three components of this model, the Prdm9 gene, intersubspecific homeology of Mus musculus musculus and Mus musculus ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302554
更新日期:2019-11-01 00:00:00
abstract::In diploid Saccharomyces cerevisiae cells, bud-site selection is determined by two cortical landmarks, Bud8p and Bud9p, at the distal and proximal poles, respectively. Their localizations depend on the multigenerational proteins Rax1p/Rax2p. Many genes involved in bud-site selection were identified previously by genom...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.128231
更新日期:2011-08-01 00:00:00
abstract::Obtaining accurate estimates of the genetic covariance matrix Sigma(G) for multivariate data is a fundamental task in quantitative genetics and important for both evolutionary biologists and plant or animal breeders. Classical methods for estimating Sigma(G) are well known to suffer from substantial sampling errors; i...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.113381
更新日期:2010-07-01 00:00:00
abstract::The RPS5 disease resistance gene of Arabidopsis mediates recognition of Pseudomonas syringae strains that possess the avirulence gene avrPphB. By screening for loss of RPS5-specified resistance, we identified five pbs (avrPphB susceptible) mutants that represent three different genes. Mutations in PBS1 completely bloc...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Use of the CRISPR/Cas9 RNA-guided endonuclease complex has recently enabled the generation of double-strand breaks virtually anywhere in the C. elegans genome. Here, we present an improved strategy that makes all steps in the genome editing process more efficient. We have created a toolkit of template-mediated repair ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.180679
更新日期:2015-10-01 00:00:00
abstract::The construction in bacteriophage lambda of a set of long DNA palindromes with paired changes in the central sequence is described. Identical palindrome centers were previously used by others to test the S-type model for cruciform extrusion in vitro. Long DNA palindromes prevent the propagation of carrier phage lambda...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::The rate of decline in reproductive fitness in populations of Drosophila melanogaster inbred at an initial rate of approximately 1% per generation has been investigated under both competitive and noncompetitive conditions. Breeding population size was variable in the inbred lines with an estimated harmonic mean of 66....
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00