Isolation and Characterization of a Bacillus cereus Mutant Strain Hyperproductive of Exo-beta-Amylase.

abstract：:Lactobacillus iners is often associated with vaginal dysbiosis and bacterial vaginosis (BV), which are risk factors for adverse gynecological and obstetric outcomes. To discover natural inhibitors of L. iners, cell-free culture supernatants (CFSs) from 77 vaginal human Lactobacillus strains and 1 human intestinal stra...

journal_title：Applied and environmental microbiology

authors： Nilsen T,Swedek I,Lagenaur LA,Parks TP

更新日期：2020-10-01 00:00:00

abstract：:The contribution of the biochemical pathways nitrification, denitrification, and dissimilatory NO3- reduction to NH4+ (DNRA) to the accumulation of NO2- in freshwaters is governed by the species compositions of the bacterial populations resident in the sediments, available carbon (C) and nitrogen (N) substrates, and e...

journal_title：Applied and environmental microbiology

authors： Kelso BHL,Smith RV,Laughlin RJ

更新日期：1999-01-01 00:00:00

abstract：:The role of the storage lipid poly-beta-hydroxybutyrate (PHB) in trichloroethylene transformation by methanotrophic mixed cultures was investigated. Naphthalene oxidation rates were used to assay for soluble methane monooxygenase activity. The PHB content of methanotrophic cells grown in reactors varied diurnally as w...

journal_title：Applied and environmental microbiology

authors： Henrysson T,McCarty PL

更新日期：1993-05-01 00:00:00

abstract：:The aromatic amino acids are synthesized via a common biosynthetic pathway. A tryptophan-producing mutant of Corynebacterium glutamicum was genetically engineered to produce tyrosine or phenylalanine in abundance. To achieve this, three biosynthetic genes encoding the first enzyme in the common pathway, 3-deoxy-d-arab...

journal_title：Applied and environmental microbiology

authors： Ikeda M,Katsumata R

更新日期：1992-03-01 00:00:00

abstract：:Binding of three Bacillus thuringiensis insecticidal crystal proteins (ICPs) to the midgut epithelium of Ostrinia nubilalis larvae was characterized by performing binding experiments with both isolated brush border membrane vesicles and gut tissue sections. Our results demonstrate that two independent ICP receptors ar...

journal_title：Applied and environmental microbiology

authors： Denolf P,Jansens S,Peferoen M,Degheele D,Van Rie J

更新日期：1993-06-01 00:00:00

abstract：:Although tractable model organisms are essential to characterize the molecular mechanisms of evolution and adaptation, the ecological relevance of their behavior is not always clear because certain traits are easily lost during long-term laboratory culturing. Here, we demonstrate that despite their long tenure in the ...

journal_title：Applied and environmental microbiology

authors： Beer KD,Wurtmann EJ,Pinel N,Baliga NS

更新日期：2014-03-01 00:00:00

abstract：:Western Lake Erie (Laurentian Great Lakes) is prone to annual cyanobacterial harmful algal blooms (cHABs) dominated by Microcystis spp. that often yield microcystin toxin concentrations exceeding the federal EPA recreational contact advisory of 8 μg liter-1 In August 2014, microcystin levels were detected in finished ...

journal_title：Applied and environmental microbiology

authors： McKindles KM,Manes MA,DeMarco JR,McClure A,McKay RM,Davis TW,Bullerjahn GS

更新日期：2020-10-28 00:00:00

abstract：:The nutristat, a substrate concentration-controlled continuous culture, was used to grow pentachlorophenol (PCP)-degrading microorganisms. The PCP concentration control system consisted of on-line measurement of the PCP concentration in the culture vessel with a tangential filter and a flowthrough spectrophotometer. W...

journal_title：Applied and environmental microbiology

authors： Rutgers M,Bogte JJ,Breure AM,van Andel JG

更新日期：1993-10-01 00:00:00

abstract：:Pseudomonas fluorescens Pf-5 is a soil bacterium that suppresses plant pathogens due in part to its production of the antibiotic pyoluteorin. Previous characterization of Pf-5 revealed three global regulators, including the stationary-phase sigma factor sigma(S) and the two-component regulators GacA and GacS, that inf...

journal_title：Applied and environmental microbiology

authors： Whistler CA,Stockwell VO,Loper JE

更新日期：2000-07-01 00:00:00

abstract：:The recent advent of tools enabling statistical inferences to be drawn from comparisons of microbial communities has enabled the focus of microbial ecology to move from characterizing biodiversity to describing the distribution of that biodiversity. Although statistical tools have been developed to compare community s...

journal_title：Applied and environmental microbiology

authors： Schloss PD,Handelsman J

更新日期：2006-10-01 00:00:00

abstract：:Soil samples were screened to select microorganisms with the capability to oxidize organic sulfides into the corresponding sulfoxides with differential enantioselectivities. Several bacterial strains that preferentially produced the S-configured sulfoxide enantiomer were isolated. Surprisingly, one bacterial strain, g...

journal_title：Applied and environmental microbiology

authors： Adam W,Heckel F,Saha-Möller CR,Taupp M,Meyer JM,Schreier P

更新日期：2005-04-01 00:00:00

abstract：:Pseudomonas oleovorans is capable of producing poly(3-hydroxyalkanoates) (PHAs) as intracellular storage material. To analyze the possible involvement of phaD in medium-chain-length (MCL) PHA biosynthesis, we generated a phaD knockout mutant by homologous recombination. Upon disruption of the phaD gene, MCL PHA polyme...

journal_title：Applied and environmental microbiology

authors： Klinke S,de Roo G,Witholt B,Kessler B

更新日期：2000-09-01 00:00:00

abstract：:The detection of Listeria monocytogenes from food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth of L. innocua can occur in samples where both species are present. In this study, we analyzed t...

journal_title：Applied and environmental microbiology

authors： Lemaître JP,Duroux A,Pimpie R,Duez JM,Milat ML

更新日期：2015-03-01 00:00:00

abstract：:Resistant and susceptible populations of the diamondback moth (Plutella xylostella) were tested with crystalline, solubilized, and partially and fully activated forms of the Bacillus thuringiensis Cry1Ac delta-endotoxin. Fully activated toxin greatly reduced the resistance ratio (ratio of the 50% lethal concentration ...

journal_title：Applied and environmental microbiology

authors： Sayyed AH,Gatsi R,Kouskoura T,Wright DJ,Crickmore N

更新日期：2001-09-01 00:00:00

abstract：:We reported recently a unique fungal structure, called the spiny ball, on the vegetative hyphae of Coprinus comatus (O. F. Müll.:Fr.) Pers. Although some observations regarding the role of this structure were presented, its function remained largely unknown. In this study, we showed that purified (isolated and washed)...

journal_title：Applied and environmental microbiology

authors： Luo H,Liu Y,Fang L,Li X,Tang N,Zhang K

更新日期：2007-06-01 00:00:00

abstract：:Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for th...

journal_title：Applied and environmental microbiology

authors： Satoh W,Nakata M,Yamamoto H,Ezaki T,Hiramatsu K

更新日期：2002-12-01 00:00:00

abstract：:Growth inhibition by isoleucine hydroxamate in Serratia marcescens was significantly enhanced by adding valine plus leucine and by using glycerol as the carbon source. Isoleucine hydroxamate-resistant mutants were isolated under conditions in which growth inhibition was enhanced. One of the mutants, strain GIHVLr2179,...

journal_title：Applied and environmental microbiology

authors： Kisumi M,Komatsubara S,Chibata I

更新日期：1977-12-01 00:00:00

abstract：:An inexpensive, simple-to-construct nephelometer which was used to monitor the lysis of spheroplasts is described. The nephelometer is a flow-through device with a linear response to cell concentration from the lower detection limit to 8 x 10(8) cells per ml. ...

journal_title：Applied and environmental microbiology

authors： Smith TL,Witter LD

更新日期：1982-12-01 00:00:00

abstract：:A technique to determine which nutrients limit bacterial growth in soil was developed. The method was based on measuring the thymidine incorporation rate of bacteria after the addition of C, N, and P in different combinations to soil samples. First, the thymidine incorporation method was tested in two different soils:...

journal_title：Applied and environmental microbiology

authors： Aldén L,Demoling F,Bååth E

更新日期：2001-04-01 00:00:00

abstract：:Blooms of the cyanobacterium Anabaena circinalis are a major worldwide problem due to their production of a range of toxins, in particular the neurotoxins anatoxin-a and paralytic shellfish poisons (PSPs). Although there is a worldwide distribution of A. circinalis, there is a geographical segregation of neurotoxin pr...

journal_title：Applied and environmental microbiology

authors： Beltran EC,Neilan BA

更新日期：2000-10-01 00:00:00

abstract：:The common reed (Phragmites australis), a cosmopolitan aquatic macrophyte, plays an important role in the structure and function of aquatic ecosystems. We compared bacterial community compositions (BCCs) and their assembly processes in the root-associated compartments (i.e., rhizosphere and endosphere) of reed and bul...

journal_title：Applied and environmental microbiology

authors： He R,Zeng J,Zhao D,Huang R,Yu Z,Wu QL

更新日期：2020-07-02 00:00:00

abstract：:Pseudomonas putida aggressively colonizes root surfaces and is agglutinated by a root surface glycoprotein. Mutants of P. putida derived chemically or by Tn5 insertion demonstrated enhanced or decreased agglutinability. Two nonagglutinable Tn5 mutants (Agg) and two mutants with enhanced agglutinability (Agg) possessed...

journal_title：Applied and environmental microbiology

authors： Anderson AJ,Habibzadegah-Tari P,Tepper CS

更新日期：1988-02-01 00:00:00

abstract：:This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology. A central composite design was used as an experimental design ...

journal_title：Applied and environmental microbiology

authors： Oh S,Rheem S,Sim J,Kim S,Baek Y

更新日期：1995-11-01 00:00:00

abstract：:The diversity of fungi in permanently frozen soil from northeastern Siberia was studied by culture-independent PCR amplification of diverse environmental 18S rRNA genes. Elaborate protocols to avoid contamination during drilling, sampling, and amplification were used. A broad diversity of eukaryotic DNA sequences that...

journal_title：Applied and environmental microbiology

authors： Lydolph MC,Jacobsen J,Arctander P,Gilbert MT,Gilichinsky DA,Hansen AJ,Willerslev E,Lange L

更新日期：2005-02-01 00:00:00

abstract：:Synthetic deoxyoligonucleotide probes that hybridized against the region at positions 455 through 480 of 16S rRNA were developed for the identification of all five Xenorhabdus species. Sequence variation in the respective rRNA region between two strains of Xenorhabdus luminescens in addition allowed the construction o...

journal_title：Applied and environmental microbiology

authors： Pütz J,Meinert F,Wyss U,Ehlers RU,Stackebrandt E

更新日期：1990-01-01 00:00:00

abstract：:The nor-1 gene in the filamentous fungus Aspergillus parasiticus encodes a ketoreductase involved in aflatoxin biosynthesis. To study environmental influences on nor-1 expression, we generated plasmid pAPGUSNNB containing a nor-1 promoter-beta-glucuronidase (GUS) (encoded by uidA) reporter fusion with niaD (encodes ni...

journal_title：Applied and environmental microbiology

authors： Chiou CH,Miller M,Wilson DL,Trail F,Linz JE

更新日期：2002-01-01 00:00:00

abstract：:Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats depo...

journal_title：Applied and environmental microbiology

authors： Oladeinde A,Bohrmann T,Wong K,Purucker ST,Bradshaw K,Brown R,Snyder B,Molina M

更新日期：2014-01-01 00:00:00

abstract：:Rhodococcus erythropolis HL 24-1 isolated as a 2,4-dinitrophenol-degrading organism can utilize 2-chloro-4,6-dinitrophenol as the sole nitrogen, carbon, and energy source under aerobic conditions. This compound is metabolized with liberation of stoichiometric amounts of chloride and nitrite. Under anaerobic conditions...

journal_title：Applied and environmental microbiology

authors： Lenke H,Knackmuss H

更新日期：1996-03-01 00:00:00

abstract：:The mechanisms of metal resistance of a cadmium-resistant Alcaligenes sp. were studied. Growth in a defined medium was unaffected by cadmium at concentrations up to 0.1 mM, while at concentrations up to 2.5 mM, growth occurred after an extended lag phase. The increase in length of the lag phase was abolished by repeat...

journal_title：Applied and environmental microbiology

authors： McEntee JD,Woodrow JR,Quirk AV

更新日期：1986-03-01 00:00:00

abstract：:Enterococcus faecalis encodes a biotin-dependent oxaloacetate decarboxylase (OAD), which is constituted by four subunits: E. faecalis carboxyltransferase subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and the novel subunit Ef-H. Our results show that in E. faecalis, subunits Ef-A, Ef-D, ...

journal_title：Applied and environmental microbiology

authors： Repizo GD,Blancato VS,Mortera P,Lolkema JS,Magni C

更新日期：2013-05-01 00:00:00