Blebbistatin, a novel inhibitor of myosin II ATPase activity, increases aqueous humor outflow facility in perfused enucleated porcine eyes.

abstract：:Posterior eye cups of the frog Rana ridibunda were prefixed in the presence of tannic acid, osmicated and stained en bloc with uranyl acetate. Electron microscopy revealed the following features of membrane traffic in cells of the retinal pigment epithelium (RPE): (1) intracellular membranes with increased electron de...

journal_title：Investigative ophthalmology & visual science

authors： Rungger-Brändle E,Englert U,Leuenberger PM

更新日期：1987-12-01 00:00:00

abstract：Purpose:To evaluate the contribution of fixational saccades toward fixation instability in strabismic monkeys. Methods:Binocular eye movements were measured as six experimental monkeys (five strabismic monkeys and one monkey with downbeat nystagmus) and one normal monkey fixated targets of two shapes (Optotype, Disk) ...

journal_title：Investigative ophthalmology & visual science

authors： Upadhyaya S,Pullela M,Ramachandran S,Adade S,Joshi AC,Das VE

更新日期：2017-11-01 00:00:00

abstract：PURPOSE:Retinal pigment epithelial (RPE) cells play important roles in ophthalmologic diseases such as proliferative vitreoretinopathy, AMD, and diabetic retinopathy. MicroRNA-34a (miR-34a) has been reported to be important in the regulation of cell proliferation, migration, differentiation, and apoptosis. In this stud...

journal_title：Investigative ophthalmology & visual science

authors： Hou Q,Tang J,Wang Z,Wang C,Chen X,Hou L,Dong XD,Tu L

更新日期：2013-10-03 00:00:00

abstract：PURPOSE:This study determined whether adenovirus-mediated transfer of the murine interferon-beta (AdCMVIFN-beta) gene protects against liver metastases arising from intraocular melanomas in mice. METHODS:A replication-deficient adenovirus vector (AdCMVIFN-beta) was used for the in vivo transfer of the murine IFN-beta ...

journal_title：Investigative ophthalmology & visual science

authors： Alizadeh H,Howard K,Mellon J,Mayhew E,Rusciano D,Niederkorn JY

更新日期：2003-07-01 00:00:00

abstract：PURPOSE:The transplantation of cultivated corneal endothelial cells (CECs) has gained attention recently for the treatment of patients with corneal endothelial dysfunction. However, an efficient culturing technique for human (H)CECs has yet to be properly established. The present study was conducted to investigate the ...

journal_title：Investigative ophthalmology & visual science

authors： Okumura N,Ueno M,Koizumi N,Sakamoto Y,Hirata K,Hamuro J,Kinoshita S

更新日期：2009-08-01 00:00:00

abstract：Purpose:To investigate the roles and pathways of microRNAs 143 and 145 in transforming growth factor (TGF)-β1-induced human subconjunctival fibrosis. Methods:Human tenon's capsule fibroblasts (HTFs) were obtained from a healthy eye. After treating cultured HTFs with TGF-β1, the expression of microRNAs 143 and 145 was ...

journal_title：Investigative ophthalmology & visual science

authors： Hwang YH,Jung SA,Lyu J,Kim YY,Lee JH

更新日期：2019-05-01 00:00:00

abstract：PURPOSE:The purpose of the present study was to identify the expression of estrogen and progesterone receptor mRNA and of estrogen and progesterone receptor protein in the conjunctiva of healthy women. METHODS:Specimens of conjunctival tissue of 10 premenopausal women (age range, 13-38 years) were obtained during opht...

journal_title：Investigative ophthalmology & visual science

authors： Fuchsjäger-Mayrl G,Nepp J,Schneeberger C,Sator M,Dietrich W,Wedrich A,Huber J,Tschugguel W

更新日期：2002-09-01 00:00:00

abstract：PURPOSE:In corneal endothelial cells, intercellular Ca(2+) waves elicited by a mechanical stimulus involve paracrine intercellular communication, mediated by ATP release via connexin hemichannels, as well as gap junctional intercellular communication. Both mechanisms are inhibited by thrombin, which activates RhoA and ...

journal_title：Investigative ophthalmology & visual science

authors： D'hondt C,Srinivas SP,Vereecke J,Himpens B

更新日期：2007-04-01 00:00:00

abstract：PURPOSE:Looping patterns rich in laminin are present in tissue samples of primary aggressive human uveal melanomas and their metastases. Because these extravascular patterns connect to blood vessels and transmit fluid in vitro and in vivo, the three-dimensional configuration of these patterns has been the subject of co...

journal_title：Investigative ophthalmology & visual science

authors： Chen X,Ai Z,Rasmussen M,Bajcsy P,Auvil L,Welge M,Leach L,Vangveeravong S,Maniotis AJ,Folberg R

更新日期：2003-07-01 00:00:00

abstract：:This study used primary cultures of conjunctival and corneal epithelial cells from rabbit, monkey and humans to investigate the infection process and host interactions of chlamydia. The epithelial cells were isolated from bulbar and palpebral conjunctivae and the cornea following incubation with EDTA or dispase and mi...

journal_title：Investigative ophthalmology & visual science

authors： Patton DL,Chan KY,Kuo CC,Cosgrove YT,Langley L

更新日期：1988-07-01 00:00:00

abstract：:Isotonic, neutral buffered solutions of benzalkonium chloride or chlorhexidine digluconate were applied topically to one eye of rabbits or human subjects. Contralateral control eyes received phosphate buffered saline as placebo. One-half hour later, the tear film of both eyes was loaded with nonpreserved sodium fluore...

journal_title：Investigative ophthalmology & visual science

authors： Burstein NL

更新日期：1984-12-01 00:00:00

abstract：PURPOSE:Heparanase and VEGF are related closely to angiogenesis in cancer. The purpose of our study was to evaluate the expression and correlation of heparanase and VEGF in hypoxia-induced retinal neovascularization. METHODS:C57BL/6 oxygen-induced retinopathy (OIR) mice and human retinal microvascular endothelial cell...

journal_title：Investigative ophthalmology & visual science

authors： Hu J,Song X,He YQ,Freeman C,Parish CR,Yuan L,Yu H,Tang S

更新日期：2012-10-03 00:00:00

abstract：PURPOSE:To isolate and characterize ciliary body epithelial antigens reactive with a monoclonal antibody, 2B4.14.1. METHODS:A mouse monoclonal antibody generated against human corneal endothelium, 2B4.14.1 reacts with nonpigmented epithelium of human and guinea pig ciliary bodies. The ciliary body proteins reactive wi...

journal_title：Investigative ophthalmology & visual science

authors： Howell DN,Ross JR,Miller SE,Sanfilippo FP

更新日期：1994-07-01 00:00:00

abstract：PURPOSE:Defensins are naturally occurring antimicrobial peptides. Recently the authors published evidence of defensin production by the human ocular surface. A study was undertaken to look for intraocular defensins that may account for unexplained antimicrobial activity of intraocular fluids. METHODS:Reverse transcrip...

journal_title：Investigative ophthalmology & visual science

authors： Haynes RJ,McElveen JE,Dua HS,Tighe PJ,Liversidge J

更新日期：2000-09-01 00:00:00

abstract：PURPOSE:Elevated intraocular pressure (IOP), sensed as mechanical stretching by trabecular meshwork (TM) cells, triggers extracellular matrix (ECM) remodeling. In addition to changes in gene expression, alternative mRNA splicing may alter ECM protein isoforms. Changes in mRNA expression and alternative splicing of four...

journal_title：Investigative ophthalmology & visual science

authors： Keller KE,Kelley MJ,Acott TS

更新日期：2007-03-01 00:00:00

abstract：:Ocular malformations and visual deficits are pathognomic of fetal alcohol syndrome (FAS). However, there are no reports on retinal visual function. To determine if prenatal (human second-trimester equivalent) or prenatal plus 10 days of postnatal (human third-trimester equivalent) ethanol exposure produced scotopic an...

journal_title：Investigative ophthalmology & visual science

authors： Katz LM,Fox DA

更新日期：1991-10-01 00:00:00

abstract：Purpose:To investigate the molecular mechanisms of the antiangiogenic activity of UPARANT, an antagonist of the urokinase-type plasminogen activator receptor (uPAR), on primary human retinal endothelial cells (HREC) as a model of in vitro angiogenesis. Methods:The antiangiogenic activity of UPARANT was evaluated on en...

journal_title：Investigative ophthalmology & visual science

authors： Motta C,Lupo G,Rusciano D,Olivieri M,Lista L,De Rosa M,Pavone V,Anfuso CD

更新日期：2016-10-01 00:00:00

abstract：PURPOSE:The authors examined the muscarinic acetylcholine receptor (mAChR) subtypes in rabbit corneal epithelial and endothelial cells and in subcellular fractions of these cell types. A Chinese hamster ovary (CHO) cell line (nontransfected CHO K1), expected to be a negative control, also was investigated. METHODS:Who...

journal_title：Investigative ophthalmology & visual science

authors： Lind GJ,Cavanagh HD

更新日期：1995-07-01 00:00:00

abstract：PURPOSE:The role of macular pigment (MP) in age-related maculopathy (ARM) is still not clearly understood. Recent studies have reported on variations in the spatial distribution of MP optical density (MPOD) including a secondary peak ("ring") in the slope of the MPOD profile. The authors investigated in a cross-section...

journal_title：Investigative ophthalmology & visual science

authors： Dietzel M,Zeimer M,Heimes B,Pauleikhoff D,Hense HW

更新日期：2011-10-11 00:00:00

abstract：PURPOSE:To determine whether changes in the retinal blood flow in light and darkness occur in humans. METHODS; The systolic and diastolic flow velocities were measured by color Doppler in the ophthalmic and the central retinal arteries in 12 healthy individuals in light and darkness. RESULTS:In the ophthalmic artery t...

journal_title：Investigative ophthalmology & visual science

authors： Havelius U,Hansen F,Hindfelt B,Krakau T

更新日期：1999-07-01 00:00:00

abstract：PURPOSE:Peroxynitrite generated during the early phase of experimental autoimmune uveoretinitis (EAU) causes peroxidation of docosahexaenoic acid (22:6), a principal unsaturated fatty acid of the photoreceptor membrane, to its hydroperoxide (22:6HP). During this phase, microglia migrate to the site of photoreceptors. T...

journal_title：Investigative ophthalmology & visual science

authors： Saraswathy S,Wu G,Rao NA

更新日期：2006-08-01 00:00:00

abstract：PURPOSE:The inhibition of angiogenesis by angiostatic steroids has been demonstrated in a variety of systems, including rabbit and rat cornea. There is considerable interest in the therapeutic potential of this class of compounds for angiogenic ocular conditions such as diabetic retinopathy, macular degeneration, and r...

journal_title：Investigative ophthalmology & visual science

authors： Penn JS,Rajaratnam VS,Collier RJ,Clark AF

更新日期：2001-01-01 00:00:00

abstract：Purpose:To compare the capability of the lamina cribrosa depth (LCD) and lamina cribrosa (LC) curvature in discriminating between eyes with primary open-angle glaucoma (POAG) and healthy eyes. Methods:Seventy-seven eyes of 77 patients with POAG and 77 eyes of 77 healthy subjects who were matched for age, sex, and axia...

journal_title：Investigative ophthalmology & visual science

authors： Lee SH,Kim TW,Lee EJ,Girard MJ,Mari JM

更新日期：2017-02-01 00:00:00

abstract：Purpose:To compare the phenotype of blue cone monochromacy (BCM) caused by large deletion mutations with those having the C203R missense mutation. Methods:BCM patients with large deletion mutations (n = 21; age range, 5-60 years), and with the C203R missense mutation (n = 13; age range, 5-70 years), were studied with ...

journal_title：Investigative ophthalmology & visual science

authors： Sumaroka A,Garafalo AV,Cideciyan AV,Charng J,Roman AJ,Choi W,Saxena S,Aksianiuk V,Kohl S,Wissinger B,Jacobson SG

更新日期：2018-12-03 00:00:00

abstract：PURPOSE:Methylenetetrahydrofolate reductase (Mthfr) is a key enzyme in homocysteine-methionine metabolism. We investigated Mthfr expression in retina and asked whether mild hyperhomocysteinemia, due to Mthfr deficiency, alters retinal neurovascular structure and function. METHODS:Expression of Mthfr was investigated a...

journal_title：Investigative ophthalmology & visual science

authors： Markand S,Saul A,Roon P,Prasad P,Martin P,Rozen R,Ganapathy V,Smith SB

更新日期：2015-04-01 00:00:00

abstract：PURPOSE:The influence of genetic and prenatal environmental factors on characteristics of saccadic performance were evaluated in young monozygotic (MZ) twins (8-19 years old) of known chorion type. METHODS:Saccadic eye movements were recorded using an infrared system. Saccadic latency, accuracy, and parameters of ampl...

journal_title：Investigative ophthalmology & visual science

authors： Blekher T,Christian JC,Abel LA,Yee RD

更新日期：1998-10-01 00:00:00

abstract：PURPOSE:To provide a clinical and genetic description of a patient cohort with Stargardt disease (STGD1) with identifiable foveal sparing. METHODS:Patients with retinal atrophy (defined as an absence of autofluorescence) that surrounded the fovea by at least 180° and did not include the fovea were defined as having fo...

journal_title：Investigative ophthalmology & visual science

authors： van Huet RA,Bax NM,Westeneng-Van Haaften SC,Muhamad M,Zonneveld-Vrieling MN,Hoefsloot LH,Cremers FP,Boon CJ,Klevering BJ,Hoyng CB

更新日期：2014-10-16 00:00:00

abstract：PURPOSE:Deposition of the macular pigment carotenoids lutein and zeaxanthin in the human retina occurs early in life. In this study, we examined the interrelationships of maternal carotenoid status and newborn infant macular pigment levels and systemic carotenoid status. As a secondary measure, we also evaluated the ef...

journal_title：Investigative ophthalmology & visual science

authors： Henriksen BS,Chan G,Hoffman RO,Sharifzadeh M,Ermakov IV,Gellermann W,Bernstein PS

更新日期：2013-08-15 00:00:00

abstract：PURPOSE:Videokeratography is a powerful tool for the diagnosis of corneal shape abnormalities. However, interpretation of the topographic map is sometimes difficult, especially when pathologies with similar topographic patterns are suspected. The neural networks model, an artificial intelligence approach, was applied f...

journal_title：Investigative ophthalmology & visual science

authors： Maeda N,Klyce SD,Smolek MK

更新日期：1995-06-01 00:00:00

abstract：PURPOSE:Several studies have reported higher levels of macular pigment (MP) in association with reduced risk for age-related macular degeneration (ARMD), a disease to which there is a genetic predisposition. A classic twin study was performed to determine the heritability of MP in the healthy eye. METHODS:One hundred ...

journal_title：Investigative ophthalmology & visual science

authors： Liew SH,Gilbert CE,Spector TD,Mellerio J,Marshall J,van Kuijk FJ,Beatty S,Fitzke F,Hammond CJ

更新日期：2005-12-01 00:00:00