Abstract：
:This study used primary cultures of conjunctival and corneal epithelial cells from rabbit, monkey and humans to investigate the infection process and host interactions of chlamydia. The epithelial cells were isolated from bulbar and palpebral conjunctivae and the cornea following incubation with EDTA or dispase and microdissection. The cells were trypsinized and grown in microtest wells or Rose Chambers (gelatin-coated glass substratum). The cell origin of the cultured epithelial cells was verified by immunofluorescence staining with anticytokeratins. The cells were nonreactive when stained with antivimentin, a fibroblast marker. The growth kinetics of the cultured epithelial cells were determined by morphological criteria. Cells reached confluency by 5-7 days, and remained as such for 18 days. The cells were maintained as long as 32 days in primary culture. After the cells had attained confluency (5-7 days), they were infected with ocular strains B/TW-5/OT or C/TW-3/OT C. trachomatis. Chlamydial inclusions were identified by light and electron microscopy. Direct FITC C. trachomatis staining (species-specific monoclonal antibody) of the inclusions was used to determine the infectivity of the various types of epithelial cells. The dynamics of the infection process was documented by time lapse photomicroscopy. Small inclusions were identified by 18 hr postinoculation (pi). By 48 hr pi, the inclusions had dramatically increased in size, occupying much of the host perikarya. The reticulate bodies were very active at this time. By 70 to 100 hr pi, the inclusions contained the highly infectious elementary bodies. At this time, rupture of the inclusion and lysis of the infected cells usually occurred.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Patton DL,Chan KY,Kuo CC,Cosgrove YT,Langley Lsubject
Has Abstractpub_date
1988-07-01 00:00:00pages
1087-95issue
7eissn
0146-0404issn
1552-5783journal_volume
29pub_type
杂志文章abstract：PURPOSE:The purpose of this study was to identify target antigens of autoimmune keratitis with a disease-prone mouse model. METHODS:BALB/c nude mice grafted with embryonic rat thymi (TG nude mice) develop various organ-localized autoimmune lesions, including keratitis. A hybridoma producing a monoclonal antibody (OT-2...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.05-0774
更新日期：2006-01-01 00:00:00
abstract：PURPOSE:Obstructive sleep apnea (OSA) syndrome generates hypertension, atherosclerosis, and endothelial and autonomic dysfunction, which may mutually interact with ocular vascular regulation. Exercise and posture changes can be used to manipulate blood pressure, ocular perfusion pressure (OPP), or both. It was hypothes...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章,随机对照试验
doi：10.1167/iovs.11-7936
更新日期：2011-12-16 00:00:00
abstract：PURPOSE:The loss of attentional visual field (AVF) has been linked to poor mobility and car crashes. We investigated the risk factors associated with a decrease in AVF over time among participants in the Salisbury Eye Evaluation Driving Study (SEEDS). METHODS:In a longitudinal cohort of 968 individuals ages 67 to 87, ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.11-8874
更新日期：2013-03-13 00:00:00
abstract：PURPOSE:To examine whether Na(+)-K(+)-2Cl(-) cotransport has the potential to contribute to corneal endothelial ion and fluid transport in cultured and fresh bovine corneal endothelial cells. METHODS:Cl- and Na+ sensitive fluorescent dyes were used to measure furosemide-dependent ion fluxes in cultured and fresh endot...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：PURPOSE:Ion channels in the ciliary epithelium play critical roles in the formation of aqueous humor in the eye. The present study identified a novel, swelling-activated K(+) current in freshly dissociated porcine pigmented ciliary epithelial cells. METHODS:Ciliary epithelial cells were freshly dissociated from porcin...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.11-7173
更新日期：2011-07-29 00:00:00
abstract：:Previous investigations have shown that corneal epithelium, migrating to cover a wound, synthesizes protein and glycoprotein at a faster rate than does normal stratified epithelium. The authors have found that the maximal rate of synthesis, as indicated by the incorporation of leucine and glucosamine, occurs 16 hr aft...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Photoreceptor-mediated mechanisms were studied in patients with a recently identified retinopathy typified by night blindness, cystoid maculopathy, and similar scotopic and photopic electroretinograms (ERGs). Dark-adapted spectral sensitivity functions were only partly explained as composites of rod and cone curves sh...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：PURPOSE:The influence of the inner wall of Schlemm's canal on aqueous outflow facility remains poorly understood. We examined the relationship between inner wall pore characteristics and outflow facility in living primate eyes in which facility had been pharmacologically increased by ethacrynic acid (ECA) infusion and ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：PURPOSE:Keratoconus disease or post-LASIK corneal ectasia are increasingly treated using UV-A/riboflavin-induced corneal collagen cross-linking (CXL). However, this treatment suffers from a lack of techniques to provide an assessment in real-time of the CXL effects. Here, we investigated the potential interest of corne...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.11-9142
更新日期：2012-08-31 00:00:00
abstract：:An arterial connection between the two internal ophthalmic arteries in the rabbit was confirmed. The consensual response to ocular injury and its relationship to this interconnecting artery was examined by transecting the interconnecting vessel and creating a monocular injury by anterior chamber paracentesis. In the r...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1979-02-01 00:00:00
abstract：:Bovine corneal endothelial cells in culture synthesize predominantly type III collagen, unlike rabbit corneal endothelial cultures which synthesize type IV collagen. In an attempt to document whether this type III collagen synthesis by bovine cells is a tissue culture-specific phenomenon, collagens synthesized by orga...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：Purpose:Synucleinopathies such as multiple system atrophy (MSA) and Parkinson's disease are associated with a variety of visual symptoms. Functional and morphological retinal aberrations are therefore supposed to be valuable biomarkers for these neurodegenerative diseases. This study examined the retinal morphology and...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.61.6.12
更新日期：2020-06-03 00:00:00
abstract：:The morphology of the apical surface of rabbit retinal pigment epithelium was studied by scanning electron microscopy from the first minute to several hours after making small nonrhegmatogenous retinal detachments (blebs). From 0 to 5 min, there were only slight changes in the homogeneous, dense mat of filamentous mic...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：PURPOSE:Compelling evidence suggests that alpha2 agonists, such as brimonidine, protect retinal ganglion cells (RGCs) from injury in a wide range of animal models. However, the mechanism of action for this protection and the physiological role of the alpha2 adrenergic system in the retina is not well understood. A majo...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.06-0890
更新日期：2007-03-01 00:00:00
abstract：PURPOSE:To determine the prevalence and incidence of epiretinal membranes (ERM) in eyes with inactive extramacular cytomegalovirus (CMV) retinitis in patients with acquired immune deficiency syndrome (AIDS). METHODS:A case-control report from a longitudinal multicenter observational study by the Studies of the Ocular ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章,多中心研究
doi：10.1167/iovs.14-14479
更新日期：2014-06-12 00:00:00
abstract：PURPOSE:To describe a method for assessment and individualized compensation of anterior segment birefringence with scanning laser polarimetry. METHODS:A scanning laser polarimeter (GDx Nerve Fiber Analyzer; Laser Diagnostic Technologies, Inc., San Diego, CA) was modified to accommodate a variable compensator. The magn...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
abstract：PURPOSE:Two noninvasive delivery strategies for VEGF/PDGF receptor tyrosine kinase inhibitors (RTKI) were explored that exploited uveal retention as a means for establishing an ocular drug depot: a single oral "loading" dose and topical administration. METHODS:Melanin binding was confirmed by centrifugation and mass s...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.12-10169
更新日期：2013-02-27 00:00:00
abstract：PURPOSE:To explore the maximum retinal vasodilation in response to repeated flicker light stimulation in relation to vasospastic propensity in healthy subjects. METHODS:Twenty-four young healthy women were grouped as vasospastic and nonvasospastic, based on their history of cold extremities and on the results of nailf...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.06-0351
更新日期：2006-09-01 00:00:00
abstract：PURPOSE:To evaluate the effect of disease severity and optic disc size on the diagnostic accuracies of optic nerve head (ONH), retinal nerve fiber layer (RNFL), and macular parameters with RTVue (Optovue, Fremont, CA) spectral domain optical coherence tomography (SDOCT) in glaucoma. METHODS:110 eyes of 62 normal subje...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.10-5546
更新日期：2011-03-10 00:00:00
abstract：PURPOSE:To map the gene for autosomal dominant cataracts (ADC) in an American white family of European descent. METHODS:Ophthalmic examinations and linkage analyses using a variety of polymorphisms were performed; two-point lod scores calculated. RESULTS:Affected individuals (14 studied) exhibited variable expressivi...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：2000-08-01 00:00:00
abstract：:The expression of receptors for Fc portion (FcR) of immunoglobulin G (IgG) and for a C3b component of complement (C3bR) by herpes simplex virus (HSV) was studied in primary cultures of rabbit corneal cells. Monolayer cultures of epithelial, stromal and endothelial cells of the rabbit cornea were infected with three st...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：PURPOSE:To evaluate how aging alters 24-hour measurements of intraocular pressure (IOP) in the sitting and supine body positions. METHODS:Fifteen older volunteers with healthy eyes (ages, 53-71 years) were each housed for 1 day in a sleep laboratory. An 8-hour accustomed sleep period was assigned to each subject. Ever...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.11-8763
更新日期：2012-01-10 00:00:00
abstract：PURPOSE:The epidermal growth factor receptor (EGFR) appears in astrocytes after neural injury. The authors' laboratory has reported the presence of EGFR in glaucomatous optic nerves. The activation of EGFR is often associated with induction of cyclooxygenase (COX)-2. In this study, the induction of COX-2 pathway in rat...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.04-1473
更新日期：2005-06-01 00:00:00
abstract：:Retinal dysplasia is a failure in normal retinal development. The morphologic sequence of the dysplastic processes was examined in fetuses and neonates from dogs affected with an inherited form of retinal dysplasia. The dysplastic change has its onset at 45-50 days' gestation and is most prominent in the dorsal peripa...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：PURPOSE:Many retinal imaging instruments use infrared wavelengths to reduce the risk of light damage. However, we have discovered that exposure to infrared illumination causes a long-lasting reduction in infrared autofluorescence (IRAF). We have characterized the dependence of this effect on radiant exposure and invest...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.13-12562
更新日期：2014-05-20 00:00:00
abstract：PURPOSE:To investigate macular photoreceptor structure in patients with inherited retinal degeneration using high-resolution images and to correlate the findings with clinical phenotypes and genetic mutations. METHODS:Adaptive optics scanning laser ophthalmoscopy (AOSLO) images of photoreceptors were obtained in 16 ey...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.06-1422
更新日期：2007-07-01 00:00:00
abstract：PURPOSE:We identified a topographic relation of localized reticular pseudodrusen (RPD) to choroidal watershed zones (CWZ) and to changes in choroidal volumes (CV). METHODS:We included 30 eyes of 30 patients with RPD in an area <10 mm(2) and no other retinal alteration (76.7 ± 6.9 years). Patients underwent spectral-do...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.13-11923
更新日期：2013-05-07 00:00:00
abstract：PURPOSE:Trefoil factor family (TFF) peptides, and in particular TFF3, are characteristic secretory products of mucous epithelia that promote antiapoptosis, epithelial migration, restitution, and wound healing. For a long time, a receptor for TFF3 had not yet been identified. However, the chemokine receptor CXCR4 has be...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.15-18129
更新日期：2016-01-01 00:00:00
abstract：PURPOSE:Activation of complement has been implicated as one of the major causes of age-related macular degeneration (AMD). Evidence is accumulating for a role of complement in other retinal diseases, such as diabetic retinopathy and proliferative vitreoretinopathy. Because of the paucity of animal models that directly ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.10-6002
更新日期：2011-05-18 00:00:00
abstract：PURPOSE:To determine the effect of interleukin (IL)-1alpha and tumor necrosis factor (TNF)-alpha on cytokine, chemokine, and receptor expression in corneal stromal cells; the effect of corneal scrape injury on monocyte chemotactic and activating factor (MCAF) expression and monocyte-macrophage influx into the stroma; a...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
