Three-dimensional reconstruction of extravascular matrix patterns and blood vessels in human uveal melanoma tissue: techniques and preliminary findings.

Abstract:

PURPOSE:Looping patterns rich in laminin are present in tissue samples of primary aggressive human uveal melanomas and their metastases. Because these extravascular patterns connect to blood vessels and transmit fluid in vitro and in vivo, the three-dimensional configuration of these patterns has been the subject of considerable speculation. In the current study, methods were devised to describe the three-dimensional configuration of looping extravascular matrix patterns in archival human uveal melanoma tissue. METHODS:Twenty-five serial 4-microm-thick sections from primary uveal melanoma tissue were labeled with fluorescence-tagged laminin and examined by confocal microscopy to generate a Z-series within each 4-microm-thick section. The z-series from each section was stacked using an immersive three-dimensional environment (ImmersaDesk; Fakespace, Kitchener, Ontario, Canada) to allow for precise alignment and compensation for distortion artifact. RESULTS:Extravascular matrix patterns that appeared to form loops in two dimensions were shown to represent thin wrappings around branching and twisting cylindrical groupings of melanoma cells. Blood vessels joined with some of these laminin-positive cylindrical wrappings. CONCLUSIONS:In this preliminary study, periodic acid-Schiff (PAS)-positive laminin-rich looping patterns in two-dimensional tissue sections appear to outline cylindrical branching packets of melanoma cells rather than spheroidal nests. The conduction of fluid through this extravascular system may provide a novel delivery system for contrast and diagnostic agents.

authors

Chen X,Ai Z,Rasmussen M,Bajcsy P,Auvil L,Welge M,Leach L,Vangveeravong S,Maniotis AJ,Folberg R

doi

10.1167/iovs.02-1333

keywords:

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

2834-40

issue

7

eissn

0146-0404

issn

1552-5783

journal_volume

44

pub_type

杂志文章
  • Silencing Toll-like receptor-9 in Pseudomonas aeruginosa keratitis.

    abstract:PURPOSE:To determine the effects of silencing Toll-like receptor (TLR) 9 signaling in Pseudomonas aeruginosa keratitis. METHODS:Corneal TLR9 mRNA levels were tested by RT-PCR in C57BL/6 (B6, susceptible) and BALB/c (resistant) mice and compared. The response of B6 mice to CpG DNA, which binds TLR9, was tested after su...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-0185

    authors: Huang X,Barrett RP,McClellan SA,Hazlett LD

    更新日期:2005-11-01 00:00:00

  • A practical method for measuring macular pigment optical density.

    abstract:PURPOSE:Increasing evidence indicates that the macular pigments (MP) protect the central retina and may retard macular disease. For that reason, a practical method for measuring MP that does not require elaborate optics and can be applied to diverse populations by operators with a modest amount of experience was develo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Wooten BR,Hammond BR Jr,Land RI,Snodderly DM

    更新日期:1999-10-01 00:00:00

  • A novel experimental mouse model of retinal detachment: complete functional and histologic recovery of the retina.

    abstract:PURPOSE:To establish an experimental mouse model of retinal detachment (RD) created by corneal puncture (CP). METHODS:Mouse corneas were punctured with a 30.5-gauge beveled needle, and the anterior chamber was penetrated. Histologic and functional changes of the retina were examined by light microscopy and electroreti...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-8241

    authors: Zeng R,Zhang Y,Shi F,Kong F

    更新日期:2012-03-26 00:00:00

  • Light and electron microscopy of the anterior chamber angle structures following surgical disinsertion of the ciliary muscle in the cynomolgus monkey.

    abstract::Light and electron microscopic studies were done on 11 cynomolgus monkey eyes which had undergone total iris removal followed by surgical disinsertion of the ciliary muscle from the scleral spur 4.7 to 14.4 months earlier. Anterior chamber perfusion to measure gross outflow facility had been performed one to nine time...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Lütjen-Drecoll E,Kaufman PL,Bárány EH

    更新日期:1977-03-01 00:00:00

  • Angiography and multifocal electroretinography show that blood supply to the pig retina may be both ipsilateral and contralateral.

    abstract:PURPOSE:We recently presented a transfemoral endovascular coiling technique for inducing experimental retinal ischemia in pigs. Substantial variation was seen in the degree of ischemia. It was hypothesized that the blood supply to the retina may originate from both the ipsilateral and contralateral ophthalmic arteries ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-12376

    authors: Morén H,Gesslein B,Undrén P,Andreasson S,Malmsjö M

    更新日期:2013-09-09 00:00:00

  • Reduction of liver metastasis of intraocular melanoma by interferon-beta gene transfer.

    abstract:PURPOSE:This study determined whether adenovirus-mediated transfer of the murine interferon-beta (AdCMVIFN-beta) gene protects against liver metastases arising from intraocular melanomas in mice. METHODS:A replication-deficient adenovirus vector (AdCMVIFN-beta) was used for the in vivo transfer of the murine IFN-beta ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.02-1147

    authors: Alizadeh H,Howard K,Mellon J,Mayhew E,Rusciano D,Niederkorn JY

    更新日期:2003-07-01 00:00:00

  • Sphingosine-1-Phosphate Is a Crucial Signal for Migration of Retina Müller Glial Cells.

    abstract:PURPOSE:Migration of Müller glial cells is enhanced in proliferative retinopathies, but the mechanisms involved are ill defined. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid synthesized by sphingosine kinase (SphK), which promotes proliferation, migration, and inflammation, acting as an intracellular media...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-16195

    authors: Simón MV,Prado Spalm FH,Politi LE,Rotstein NP

    更新日期:2015-09-01 00:00:00

  • Rho/ROCK signaling in regulation of corneal epithelial cell cycle progression.

    abstract:PURPOSE:The authors' previous study showed that the expression of a Rho-associated serine/threonine kinase (ROCK) is regulated during cell cycle progression in corneal epithelial cells. The present study was conducted to determine whether and how Rho/ROCK signaling regulates cell cycle progression. METHODS:Rabbit corn...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.07-0488

    authors: Chen J,Guerriero E,Lathrop K,SundarRaj N

    更新日期:2008-01-01 00:00:00

  • Microkeratome-assisted preparation of ultrathin grafts for descemet stripping automated endothelial keratoplasty.

    abstract:PURPOSE:To compare three microkeratome-assisted techniques for the preparation of ultrathin (UT) grafts for Descemet stripping automated endothelial keratoplasty. METHODS:After dissection with a 300-μm microkeratome head in 40 donor tissues, a second cut was performed with a 130-μm head either after manual stromal hyd...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-7753

    authors: Busin M,Patel AK,Scorcia V,Ponzin D

    更新日期:2012-01-31 00:00:00

  • Therapeutic potential of semaphorin 3E for the treatment of choroidal neovascularization.

    abstract:PURPOSE:We investigated the effects of semaphorin 3E (Sema3E)/PlexinD1 signaling in the development of choroidal neovascularization (CNV) and explored the therapeutic potential of the pathway. METHODS:We used a laser-induced CNV model in the mouse. The expression of Sema3E and PlexinD1 was evaluated with immunohistoch...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-14087

    authors: Suda K,Guo C,Oishi A,Ikeda S,Uemura A,Yoshimura N

    更新日期:2014-07-01 00:00:00

  • Cone degeneration following rod ablation in a reversible model of retinal degeneration.

    abstract:PURPOSE:Amphibian retinas regenerate after injury, making them ideal for studying the mechanisms of retinal regeneration, but this leaves their value as models of retinal degeneration in question. The authors asked whether the initial cellular changes after rod loss in the regenerative model Xenopus laevis mimic those ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-5347

    authors: Choi RY,Engbretson GA,Solessio EC,Jones GA,Coughlin A,Aleksic I,Zuber ME

    更新日期:2011-01-21 00:00:00

  • Nocturnal elevation of intraocular pressure is detectable in the sitting position.

    abstract:PURPOSE:When intraocular pressure (IOP) was monitored in supine healthy young adults throughout a 24-hour period, a diurnal-to-nocturnal elevation of IOP was observed. This study was undertaken to investigate whether a similar elevation of IOP can be detected when experimental subjects are in the sitting position. MET...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.03-0349

    authors: Liu JH,Bouligny RP,Kripke DF,Weinreb RN

    更新日期:2003-10-01 00:00:00

  • Assessment of Transepidermal Water Loss From the Ocular Area in Dry Eye Disease.

    abstract:Purpose:To investigate transepidermal water loss (TEWL) from the ocular area in dry eye disease (DED) and evaluate the correlation between ocular TEWL and other DED parameters. Methods:Transepidermal water loss from the ocular area in 56 eyes with DED and 38 healthy eyes was measured using a Tewameter TM300 that was e...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.16-20109

    authors: Jeon HS,Youn SW,Jeon HE,Kim JH,Hyon JY

    更新日期:2016-09-01 00:00:00

  • Type XVII collagen (BP 180) in the developing avian cornea.

    abstract:PURPOSE:Previous sequence analyses of hemidesmosomal BP 180/collagen XVII cDNA from human skin and of a similar chicken corneal cDNA showed some similarities, but major differences as well. The authors examined whether, in one species, the same mRNA is present in cornea and skin. They also studied the developmental exp...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Gordon MK,Fitch JM,Foley JW,Gerecke DR,Linsenmayer C,Birk DE,Linsenmayer TF

    更新日期:1997-01-01 00:00:00

  • miRNA Copy Number Variants Confer Susceptibility to Acute Anterior Uveitis With or Without Ankylosing Spondylitis.

    abstract:Purpose:To investigate the association of microRNA (miRNA) copy number variants (CNVs) with acute anterior uveitis (AAU) with or without ankylosing spondylitis (AS) and to assess underlying disease mechanisms. Methods:This study included 768 patients with AAU+AS+ or AAU+AS- and 660 controls from a Chinese Han populati...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.16-21047

    authors: Yang L,Du L,Yue Y,Huang Y,Zhou Q,Cao S,Qi J,Liang L,Wu L,Wang C,Ye Z,Tian Y,Kijlstra A,Hou S,Yang P

    更新日期:2017-04-01 00:00:00

  • Distribution of transcript and protein isoforms of the synaptic glycoprotein neuroplastin in rat retina.

    abstract:PURPOSE:To examine the expression and localization of the neuroplastins (np), two synapse-enriched members of the immunoglobulin (Ig) superfamily of cell-adhesion molecules, in the developing and adult retina and optic nerve. METHODS:Expressions of the two isoforms np55 and np65 and carboxyl-terminal splice variants w...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Kreutz MR,Langnaese K,Dieterich DC,Seidenbecher CI,Zuschratter W,Beesley PW,Gundelfinger ED

    更新日期:2001-07-01 00:00:00

  • Klf4 regulates the expression of Slurp1, which functions as an immunomodulatory peptide in the mouse cornea.

    abstract:PURPOSE:The secreted Ly6/uPAR-related protein-1 (Slurp1), associated with the hyperkeratotic disorder mal de Meleda, is abundantly expressed in corneas. Here, we examine its corneal expression and functions. METHODS:Gene expression was quantified by quantitative PCR (qPCR), immunoblots, and immunofluorescent staining....

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.12-10759

    authors: Swamynathan S,Buela KA,Kinchington P,Lathrop KL,Misawa H,Hendricks RL,Swamynathan SK

    更新日期:2012-12-19 00:00:00

  • Sodium 4-phenylbutyrate acts as a chemical chaperone on misfolded myocilin to rescue cells from endoplasmic reticulum stress and apoptosis.

    abstract:PURPOSE:To evaluate the effect of chemical chaperones on the trafficking of secretion-incompetent primary open-angle glaucoma-associated mutant myocilin and the possibility to rescue cells coexpressing mutant and wild-type myocilin from endoplasmic reticulum (ER) stress and apoptosis. METHODS:CHO-K1, HEK293 and human ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-0943

    authors: Yam GH,Gaplovska-Kysela K,Zuber C,Roth J

    更新日期:2007-04-01 00:00:00

  • Visual acuity and pupillary responses to spatial structure in infants.

    abstract:PURPOSE:To determine the age of onset of the pupil grating response (PGR). To compare estimates of resolution acuity obtained by pupillometric and behavioral methods in early infancy. METHODS:Dynamic infrared pupillometry was undertaken on 19 newborn infants while they fixated a uniform background upon which a 0.1 c/d...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Cocker KD,Moseley MJ,Bissenden JG,Fielder AR

    更新日期:1994-04-01 00:00:00

  • Myeloid body associations in the frog pigment epithelium.

    abstract::Myeloid bodies are found in the retinal pigment epithelium of certain vertebrate species. They are organized structural forms of the smooth endoplasmic reticulum which are usually seen as stacks of flattened, smooth saccules having a circular or lens-shaped configuration. Our findings in the frog Rana pipiens suggest ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Matthes MT,Basinger SF

    更新日期:1980-03-01 00:00:00

  • Retarding photoreceptor degeneration in Pdegtm1/Pdegtml mice by an apoptosis suppressor gene.

    abstract:PURPOSE:Mice (Pdegtm1/Pdegtm1) homozygous for a mutant allele of the gamma subunit of retinal cyclic guanosine monophosphate phosphodiesterase (PDE gamma) suffer a severe photoreceptor degeneration. To determine whether the antiapoptotic BCL2 gene is effective in delaying the cell death pathway in this new strain of mu...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Tsang SH,Chen J,Kjeldbye H,Li WS,Simon MI,Gouras P,Goff SP

    更新日期:1997-04-01 00:00:00

  • eNOS, a pressure-dependent regulator of intraocular pressure.

    abstract:PURPOSE:Pathology in the primary drainage pathway for aqueous humor in the eye is responsible for ocular hypertension, the only treatable risk factor in patients with glaucoma. Unfortunately, the mechanisms that regulate pressure-dependent drainage of aqueous humor and thus intraocular pressure (IOP) are unknown. To be...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-7839

    authors: Stamer WD,Lei Y,Boussommier-Calleja A,Overby DR,Ethier CR

    更新日期:2011-12-09 00:00:00

  • Elastin content of the scleral spur, trabecular mesh, and sclera.

    abstract::The scleral spur and trabecular mesh of the human eye contain approximately 5% elastic tissue. Elastic tissue forms less than 2% of the sclera. ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Moses RA,Grodzki WJ Jr,Starcher BC,Galione MJ

    更新日期:1978-08-01 00:00:00

  • Reduced toxicity of liposome-associated amphotericin B injected intravitreally in rabbits.

    abstract::The ocular toxicity of liposome-intercalated amphotericin B and commercial amphotericin B were compared after intravitreal injection in healthy pigmented rabbits. Ophthalmoscopic observations over 5 weeks following a single intravitreal injection showed vitreal band formation and focal retinal damage after doses of co...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Tremblay C,Barza M,Szoka F,Lahav M,Baum J

    更新日期:1985-05-01 00:00:00

  • Comparative assessment for the ability of Cirrus, RTVue, and 3D-OCT to diagnose glaucoma.

    abstract:PURPOSE:We compared the ability of circumpapillary retinal nerve fiber layer (cpRNFL) thickness and macular parameters obtained by three spectral-domain optical coherence tomography (SD-OCT) instruments to detect glaucoma. METHODS:We enrolled 87 normal eyes and 145 glaucomatous eyes (75 early glaucomatous eyes (EGs), ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.12-11268

    authors: Akashi A,Kanamori A,Nakamura M,Fujihara M,Yamada Y,Negi A

    更新日期:2013-07-10 00:00:00

  • Protective effect of pioglitazone on retinal ischemia/reperfusion injury in rats.

    abstract:PURPOSE:To investigate the protective effect of pioglitazone on the rat retina after ischemia/reperfusion (I/R) injury and to explore its possible mechanisms. METHODS:Retinal ischemia was induced by increasing the intraocular pressure to 110 mm Hg for 60 minutes, and pioglitazone was delivered 3 hours before the I/R. ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-11614

    authors: Zhang XY,Xiao YQ,Zhang Y,Ye W

    更新日期:2013-06-06 00:00:00

  • Age-related cataracts in alpha3Cx46-knockout mice are dependent on a calpain 3 isoform.

    abstract:PURPOSE:Previous studies have demonstrated that in 129alpha3Cx46-/- mice, age-related nuclear cataract is formed. In the present study, a more in vivo-relevant model was generated to test the hypothesis that the calpain 3 gene is involved in age-related nuclear cataractogenesis in alpha3Cx46 knockout mice. METHODS:To ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-0926

    authors: Tang Y,Liu X,Zoltoski RK,Novak LA,Herrera RA,Richard I,Kuszak JR,Kumar NM

    更新日期:2007-06-01 00:00:00

  • Connexin 50-R205G Mutation Perturbs Lens Epithelial Cell Proliferation and Differentiation.

    abstract:Purpose:To investigate the underlying mechanisms for how the mouse Cx50-R205G point mutation, a homologue of the human Cx50-R198W mutation that is linked to cataract-microcornea syndrome, affects proper lens growth and fiber cell differentiation to lead to severe lens phenotypes. Methods:EdU labeling, immunostaining, ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.61.3.25

    authors: Tjahjono N,Xia CH,Li R,Chu S,Wang J,Gong X

    更新日期:2020-03-09 00:00:00

  • Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors.

    abstract::PURPOSE. Simple sphingolipids control crucial cellular processes in several cell types. Previous work demonstrated that sphingolipids, such as ceramide, sphingosine, and sphingosine-1-phosphate, are key mediators in the regulation of survival, differentiation, and proliferation of retina photoreceptors. Ceramide-1-pho...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-7065

    authors: Miranda GE,Abrahan CE,Agnolazza DL,Politi LE,Rotstein NP

    更新日期:2011-08-22 00:00:00

  • Gene transfer to the human trabecular meshwork by anterior segment perfusion.

    abstract:PURPOSE:To determine whether adenovirus vectors are capable of transferring a foreign active protein to the perfused anterior segment of the human eye. METHODS:Primary cultures from the human trabecular meshwork tissue were exposed to replication-deficient adenovirus Av1LacZ4 carrying the reporter beta-galactosidase g...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Borrás T,Matsumoto Y,Epstein DL,Johnson DH

    更新日期:1998-07-01 00:00:00