Abstract:
:Cofilin-actin rods are inclusion-like structures that are induced by certain chemical or physical stresses in cultured cells, and the rods formed in neurons are thought to be associated with neurodegeneration. Here, we cloned an Aplysia cofilin homolog and overexpressed it in cultured neurons. Overexpressed cofilin formed rod-like structures that included actin. The overall neuronal morphology was unaffected by cofilin overexpression; however, a decrease in number of synaptic varicosities was observed. Consistent with this structural change by cofilin overexpression, the synaptic strength was reduced, and furthermore, the long-term facilitation elicited by repeated pulses of 5-hydroxytryptamine was impaired in sensory-to-motor synapses. However, cofilin overexpression did not induce programmed cell death. These findings suggest that the formation of cofilin-actin rod-like structures can lead to neurodegeneration, and this might be a mechanism of rundown of neuronal and synaptic function without cell death in neurodegenerative diseases.
journal_name
Proc Natl Acad Sci U S Aauthors
Jang DH,Han JH,Lee SH,Lee YS,Park H,Lee SH,Kim H,Kaang BKdoi
10.1073/pnas.0507675102keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
16072-7issue
44eissn
0027-8424issn
1091-6490pii
0507675102journal_volume
102pub_type
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