Abstract:
AIM:To evaluate whether intratumoral injection of liposome-endostatin complexes could enhance the antitumor efficacy of radiation therapy in human liver carcinoma (BEL7402) model. METHODS:Recombinant plasmid pcDNA3.End was transfected into human liver carcinoma cell line (BEL7402) with lipofectamine to produce conditioned medium. Then BEL7402 cells and human umbilical vein endothelial cells (HUVECs) were treated with the conditioned medium. Cell cycle and apoptosis were analyzed by flow cytometer and endothelial cell proliferation rates were determined by MTT assay. The antitumor efficacy of endostatin gene combined with ionizing radiation in mouse xenograft liver tumor was observed. RESULTS:Endostatin significantly suppressed the S phase fraction and increased the apoptotic index in HUVECs. In contrast, endostatin treatment had no effect on BEL7402 cell apoptosis (2.1+/-0.3% vs 8.9+/-1.3%, t = 8.83, P = 0.009<0.01) or cell cycle distribution (17.2+/-2.3% vs 9.8+/-1.2%, t = 4.94, P = 0.016<0.05). The MTT assay showed that endostatin significantly inhibited the proliferation of HUVECs by 46.4%. The combination of local endostatin gene therapy with radiation therapy significantly inhibited the growth of human liver carcinoma BEL7402 xenografts, the inhibition rate of tumor size was 69.8% on d 28 compared to the untreated group. The tumor volume in the pcDNA3.End combined with radiation therapy group (249+/-83 mm3) was significantly different from that in the untreated group (823+/-148 mm3, t = 5.86, P = 0.009<0.01) or in the pcDNA3 group (717+/-94 mm3, t = 6.46, P = 0.003<0.01). Endostatin or the radiation alone also inhibited the growth of liver tumor in vivo, but their inhibition effects were weaker than those of endostatin combined with radiation, the inhibition rates on d 28 were 44.7% and 40.1%, respectively. CONCLUSION:Endostatin not only significantly suppresses tumor growth but also enhances the antitumor efficacy of radiation therapy in human carcinoma xenograft.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Zheng AQ,Song XR,Yu JM,Wei L,Wang XWdoi
10.3748/wjg.v11.i28.4439keywords:
subject
Has Abstractpub_date
2005-07-28 00:00:00pages
4439-42issue
28eissn
1007-9327issn
2219-2840journal_volume
11pub_type
杂志文章abstract:AIM:To evaluate the effect of sitagliptin vs placebo on histologic and non-histologic parameters of non-alcoholic steatohepatitis (NASH). METHODS:Twelve patients with biopsy-proven NASH were randomized to sitagliptin (100 mg daily) (n = 6) or placebo (n = 6) for 24 wk. The primary outcome was improvement in liver fibr...
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pub_type: 杂志文章,随机对照试验
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更新日期:2017-01-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v26.i38.5863
更新日期:2020-10-14 00:00:00
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doi:10.3748/wjg.v9.i4.683
更新日期:2003-04-01 00:00:00
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更新日期:2015-10-14 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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pub_type: 杂志文章,多中心研究
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更新日期:2015-04-28 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2019-02-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.4037
更新日期:2009-08-28 00:00:00
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pub_type: 杂志文章
doi:10.3748/wjg.v12.i29.4745
更新日期:2006-08-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i7.1060
更新日期:2007-02-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 社论
doi:10.3748/wjg.v23.i15.2635
更新日期:2017-04-21 00:00:00
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pub_type: 杂志文章
doi:10.3748/wjg.v20.i25.8253
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更新日期:2020-08-28 00:00:00
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pub_type: 杂志文章,评审
doi:10.3748/wjg.v26.i16.1888
更新日期:2020-04-28 00:00:00
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pub_type: 杂志文章
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更新日期:2011-01-21 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2006-07-21 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2014-10-07 00:00:00
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更新日期:2005-01-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v3.i2.68
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更新日期:2008-05-28 00:00:00