Abstract:
:The Arabidopsis thaliana THI1 protein is involved in thiamine biosynthesis and is targeted to both chloroplasts and mitochondria by N-terminal control regions. To investigate thi1 expression, a series of thi1 promoter deletions were fused to the beta-glucuronidase (GUS) reporter gene. Transgenic plants were generated and expression patterns obtained under different environmental conditions. The results show that expression derived from the thi1 promoter is detected early on during development and continues throughout the plant's life cycle. High levels of GUS expression are observed in both shoots and roots during vegetative growth although, in roots, expression is restricted to the vascular system. Deletion analysis of the thi1 promoter region identified a region that is responsive to light. The smallest fragment (designated Pthi322) encompasses 306 bp and possesses all the essential signals for tissue specificity, as well as responsiveness to stress conditions such as sugar deprivation, high salinity, and hypoxia.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Ribeiro DT,Farias LP,de Almeida JD,Kashiwabara PM,Ribeiro AF,Silva-Filho MC,Menck CF,Van Sluys MAdoi
10.1093/jxb/eri168keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
1797-804issue
417eissn
0022-0957issn
1460-2431pii
eri168journal_volume
56pub_type
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