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High pregnancy rates can be achieved after freezing and thawing human blastocysts.

Abstract：

OBJECTIVE:To examine the results of a 3-year trial using blastocyst cryopreservation to limit multiple pregnancy and optimize overall pregnancy per cycle. DESIGN:Retrospective clinical evaluation of pregnancy rates after freezing and thawing human blastocysts. SETTING:Tertiary-care academic center. PATIENT(S):Seven hundred fifty-three different patients treated in 783 IVF cycles with blastocysts frozen from July 2000 to June 2003. INTERVENTION(S):Two thousand, two hundred fifty-nine blastocysts were frozen in cycles in which only blastocysts were cryopreserved (cycles with pronuclear stage oocytes or pre-embryos also cryopreserved were excluded from the analysis). Of these, 628 (27.6%) were thawed in 218 cycles. MAIN OUTCOME MEASURE(S):Pregnancy rate per cycle with thaw. RESULT(S):Four hundred seventy-nine (76.3%) blastocysts survived thawing, and 440 (92.0%) were transferred after exhibiting evidence of survival (most commonly, blastocoele reexpansion). In cycles with a thaw, 211 (96.8%) of 218 underwent intrauterine transfer. An average of 2.09 blastocysts was transferred per replacement. One hundred twenty-five (59.2%) clinical pregnancies were established, which included 23 sets of twins and 5 triplet gestations. Two sets of monozygotic twins were identified after the replacement of a single thawed blastocyst (1.6%). The age of the patient at the time of cryopreservation (<37 years) was an important factor in the establishment of clinical and ongoing pregnancy. The mode of ovarian stimulation, replacement method, and whether blastocysts were frozen on day 5 or day 6 of development did not demonstrate clinical significance. CONCLUSION(S):Cryopreserved and thawed blastocysts demonstrated a similar potential for implantation when compared with fresh pre-embryos on day 3. On the basis of these results, the blastocyst stage of development appears to be optimal for clinical freeze-thaw trials.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Veeck LL,Bodine R,Clarke RN,Berrios R,Libraro J,Moschini RM,Zaninovic N,Rosenwaks Z

doi

10.1016/j.fertnstert.2004.03.068

keywords:

subject

Has Abstract

pub_date

2004-11-01 00:00:00

pages

1418-27

issue

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(04)02262-9

journal_volume

pub_type

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High pregnancy rates can be achieved after freezing and thawing human blastocysts.