Abstract:
:If real-time PCR is to be of much worth to its user, some idea regarding the reliability of its data is essential. We discuss here some of the problems associated with interpreting numerical real-time PCR data that lend themselves to analytical evaluation. We translate into the language of molecular biology some of the criteria which are used to evaluate the performance of any new method (linearity, precision, specificity, limit of detection and quantification).
journal_name
J Biol Regul Homeost Agentsjournal_title
Journal of biological regulators and homeostatic agentsauthors
Vaerman JL,Saussoy P,Ingargiola Ikeywords:
subject
Has Abstractpub_date
2004-04-01 00:00:00pages
212-4issue
2eissn
0393-974Xissn
1724-6083journal_volume
18pub_type
杂志文章abstract::Transmembrane proteases (i.e. membrane-associated proteases, ectoproteases) are present in a wide variety of tissues and cell types including endothelial, epithelial and hematopoietic cells. Natural and synthetic inhibitors have been characterized and have revealed that certain ectoenzymes are able to modulate bioacti...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2001-04-01 00:00:00
abstract::To compare the effectiveness of Doctor Tecar TherapyTM with that of laser therapy in the management of low back pain (LBP), a total of 60 patients with LBP were enrolled. The participants were randomly divided into two groups: a Tecar group (experimental group, 30 subjects), and a laser group (control group, 30 subjec...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2017-04-01 00:00:00
abstract::Regulated on activation, normal T-cell expressed and presumably secreted (RANTES), which generally mediates monocyte-macrophage (MO) activation and recruitment, is a protein of 8-10 kD that chemoattracts eosinophils, monocytes and certain T leukocyte subsets. RANTES is coded for by a gene cluster located on human chro...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Effects of recombinant human interferon alpha (HuIFN-alpha) on human megakaryocyte (CFU-MK) and fibroblast (CFU-F) colony-forming cell growth were studied. Concentration-dependent inhibition of both CFU-MK and CFU-F by HuIFN-alpha was demonstrated. Statistically significant suppression of both CFU-MK and CFU-F was see...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::Many studies demonstrated that human adult cardiac progenitor cells in the form of cardiospheres (CSps) could represent a powerful candidate for cardiac cell therapy. To achieve the clinical translation of this biotechnological product, the development of well-defined culture conditions is required to optimize their p...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2011-04-01 00:00:00
abstract::Dental pulp stem cells (DPSCs) are multipotent stem cells with the potential to differentiate into various cell types. For this reason, they have been proposed as an alternative source for mesenchymal stem cells. Somatostatin is a peptide hormone with an inhibitory effect on several endogenous hormones. The aim of our...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2015-07-01 00:00:00
abstract::Pelvic floor disorders represent a significant cause of morbidity associated with a severe reduction of quality of life. It represents a very common clinical problem that afflicts women three to seven time more often than men. The purpose of this study was to assess the diagnostic tools available to define the imaging...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2013-07-01 00:00:00
abstract::Heat-induced total body hyperthermia has been shown to synergistically enhance the in vivo antitumor effects of MuIFN-gamma directed against B16 melanoma in mice. This observation was made with an 8-hour exposure to hyperthermia that was administered following MuIFN-gamma treatment. It was not known whether this was t...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Upper respiratory infections are widespread in clinical practice. Antibiotics are frequently used in the management of patients with airways infection. However, antibiotics can induce intestinal and respiratory dysbiosis that, in turn, worsens respiratory symptoms. Moreover, respiratory infections per se can cause dys...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2020-11-01 00:00:00
abstract::The systematic analysis of parameters impacting implant primary stability is difficult to achieve with human cadavers or animal models, particularly for complex trans-sinus procedures to determine the effects of cortical layers and bone engagement on implant stability before and after a simulated load in vitro. Solid ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2020-11-01 00:00:00
abstract::A potential role of Helicobater Pylori (HP) infection in several extra-intestinal pathologies has been recently suggested. The aim of our study was to assess the role of serology positive for HP in atopic and non atopic infants and children affected by atopic dermatitis, urticaria, rhinitis and asthma. We included 615...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::This study was carried out to investigate the expression changes of RanBP9 in tissue specimens and osteosarcoma cell strains and preliminarily explore its mechanism in osteosarcoma, so as to provide a theoretical foundation for follow-up experiments. The expression of RanBP9 in human osteosarcoma tissue specimens was ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::Microscopic satellites (microsatellites) in primary melanoma are defined as one or more discontinuous nests of neoplastic melanocytes measuring more than 0.05 mm in diameter that are clearly separated by normal dermis (i.e., no fibrosis or inflammation) from the main invasive component of the melanoma by a distance of...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::Cartilage lesions still represent an unsolved problem: despite the efforts of the basic and translational research, the regeneration of this tissue is far from being reached (1-3). Articular cartilage lesions can be divided in two main groups: superficial or partial defects and full-thickness defects (4, 5). Partial l...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2018-11-01 00:00:00
abstract::The transcription of HPV-16 E6/E7 RNA has been studied by the polymerase chain reaction (PCR) in the Siha cervical carcinoma cell line. With this technique, all three different transcripts have been detected: the cDNA colinear E6 form, and the two differentially spliced forms E6*I and E6*II. All types of RNA appear le...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::The precise etiology of Inflammatory Bowel Disease (IDB) remains unclear and several factors are believed to play a role in its development and progression, including the composition of microbial communities resident in the gastrointestinal tract. Human intestinal microbiota are extensive with at least 15,000-36,000 b...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-01-01 00:00:00
abstract::There is a great hazard of mercury intoxication in the third world for artisanal miners using mercury as amalgam for extracting and refining gold. In developing countries, there is the possibility of risk regarding exposure to Hg from amalgam tooth fillings, ethyl-Hg (thimerosal) added as antiseptic to vaccines and me...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论,评审
doi:
更新日期:2009-10-01 00:00:00
abstract::Aim of this retrospective study was to evaluate the one-year clinical and radiographic outcomes of implants with a triangular shaped neck inserted immediately after tooth extraction in esthetic zones. Patients in which immediate postextraction implants were placed and restored in the anterior maxilla, who underwent a ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2019-11-01 00:00:00
abstract::The pseudoarthrosis (PSA) of scaphoid leads to alteration in load transfer in the wrist joint. Its treatment aims to achieve consolidation to improve clinical complaints and prevent post-traumatic arthritis. The indication for using vascularized bone grafts is still controversial. This prospective comparative study ai...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 临床试验
doi:
更新日期:2020-05-01 00:00:00
abstract::Different surgical techniques have been developed to reconstruct the posterior maxilla without bone graft. A barrier membrane usually placed internal to the sinus, without stabilizer or bone window, pushed inside the sinus cavity as the ''roof'' of the sinus cavity to preserve the space and help bone regeneration has ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2018-11-01 00:00:00
abstract::Reflectance confocal microscopy (RCM) is a non-invasive, in vivo technique for real-time imaging of the epidermis and superficial dermis at the cellular resolution. We performed a pilot study focusing on the evaluation of the effect of topical corticosteroids and calcineurin inhibitors on the epidermis of patients wit...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 临床试验,信件
doi:
更新日期:2015-07-01 00:00:00
abstract::We recently identified a rheumatoid factor associated with autoimmune disease resistance and remission, and have named it regulatory rheumatoid factor (regRF). Epitopes recognized by regRF can be induced in papain Fc fragments of IgG. Immunization of arthritic rats with homologous Fc fragments that expose neoepitopes ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2019-05-01 00:00:00
abstract::Free radical damage to many cellular components has been proposed as the main mechanism underlying the aging process. In the liver, NO can be generated by iNOS, but also by the constitutively expressed endothelial NOS (eNOS). iNOS enzyme appears to be expressed in liver disease such as cirrhosis and fulminant hepatiti...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Cancer of the oral cavity is known to have a diverse aetiology that includes infectious agents. Human papilloma virus has been found to be associated with several types of human cancer, inclusive of cervical, vulvar, vaginal, penile, anal, and cancer of tonsil. The aim of this manuscript is to investigate the presence...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2018-01-01 00:00:00
abstract::The early detection of genomic biomarkers (e.g. RNAs) through analysis of circulating blood cells could have a substantial impact on biomedicine, particularly in monitoring clinical trials, drug toxicity and doping in athletes. To achieve this goal, it is essential to develop methods that are sufficiently sensitive to...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2010-10-01 00:00:00
abstract::Intravenous IgG has been adopted as treatment for various immune-related diseases, including immune thrombocytopenic purpura, autoimmune neuropathies, systemic lupus erythematosus, Guillain-Barré syndrome, myasthenia gravis, Kawasaki disease, skin blistering diseases. The intravenous administration of exogenously pool...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论
doi:
更新日期:2009-01-01 00:00:00
abstract::This study was carried out by varying both graphene oxide (GO) concentration (10 μg/mL, 50 μg/mL, 100 μg/mL) and flakes sizes of 1320 nm and 130 nm. Characterization by scanning electron microscopy and Raman spectroscopy demonstrate that the area of GO flakes varies of one order of magnitude but their c...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00
abstract::Twinky Star colored compomer produced by VOCO GmbH is a new type of filling material designed with seven different colors. Previous studies have shown that using the colored filling material can induce childrens curiosity and increase the acceptance for dental treatment. However, few studies have been conducted to ev...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
abstract::Mast cells (MCs) are mediators of allergy and inflammation and participate in the growth of cancer cells. MCs can promote both neoangiogenesis and tumor growth. They increase in the stroma of certain tumors where they can be recruited by tumor-derived chemoattractants, such as monocyte chemotactic protein-1 (MCP-1), R...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论
doi:
更新日期:2016-10-01 00:00:00
abstract::A cross-talk between dendritic cells (DC) and resting natural killer (NK) cells leads to the activation of both cell populations, a process requiring cell-cell contact. When the number of activated NK cells overwhelms surrounding DC, they became able to kill specifically immature DC, a feedback mechanism to shut off D...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2002-01-01 00:00:00