Regulation of Indian hedgehog mRNA levels in chondrocytic cells by ERK1/2 and p38 mitogen-activated protein kinases.


:Indian hedgehog (Ihh) is produced by growth plate pre-hypertrophic chondrocytes, and is an important regulator of endochondral ossification. However, little is known about the regulation of Ihh in chondrocytes. We have examined the role of integrins and mitogen-activated protein (MAP) kinases in Ihh mRNA regulation in CFK-2 chondrocytic cells. Cells incubated with the beta1-integrin blocking antibody had decreased Ihh mRNA levels, which was accompanied by decreases of activated extracellular signal-regulated kinases (ERK1/2) and activated p38 MAPK. Ihh mRNA levels were also inhibited by U0126, a specific MEK1/2 inhibitor, or SB203580, a specific p38 MAPK inhibitor. Cells transfected with constitutively active MEK1 or MKK3 had increased Ihh mRNA levels, which were diminished by dominant-negative MEK1, p38alpha or p38beta. Stimulation of the PTH1R with 10(-8) M rPTH (1-34) resulted in dephosphorylation of ERK1/2 that was evident within 15 min and sustained for 1 h, as well as transient dephosphorylation of p38 MAPK that was maximal after 25 min. PTH stimulation decreased Ihh mRNA levels, and this effect was blocked by transfecting the cells with constitutively active MEK1 but not by MKK3. These studies demonstrated that activation of ERK1/2 or p38 MAPK increased Ihh mRNA levels. Stimulation of the PTH1R or blocking of beta1-integrin resulted in inhibition of ERK1/2 and p38 MAPK and decreased levels of Ihh mRNA. Our data demonstrate the central role of MAPK in the regulation of Ihh in CFK-2 cells.


J Cell Physiol


Lai LP,DaSilva KA,Mitchell J





Has Abstract


2005-04-01 00:00:00












  • The increase in hormone-stimulated adenylate cyclase activity following Rous sarcoma virus transformation.

    abstract::Rous sarcoma virus (RSV)-infected chicken embryo cells were used to study the effect of viral transformation on the hormone-stimulated synthesis of cyclic AMP. Transformation by RSV greatly increased the cells' ability to synthesize and accumulate cyclic AMP in response to the beta-adrenergic agonist isoproterenol as ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Howard RF,Friis RR,Sheppard JR,Glossmann H

    更新日期:1982-06-01 00:00:00

  • Fibroblast-dependent growth of mouse mast cells in vitro: duplication of mast cell depletion in mutant mice of W/Wv genotype.

    abstract::In spite of the apparent depletion of mast cells in tissues of mutant mice of W/Wv genotype, cells with many features of mast cells do develop when bone marrow cells of W/Wv mice are cultured in the presence of pokeweed mitogen-stimulated spleen cell-conditioned medium (PWM-SCM). In order to resolve this discrepancy a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Fujita J,Nakayama H,Onoue H,Kanakura Y,Nakano T,Asai H,Takeda S,Honjo T,Kitamura Y

    更新日期:1988-01-01 00:00:00

  • Adenosine and adenosine receptors in the immunopathogenesis and treatment of cancer.

    abstract::Tumor cells overcome anti-tumor responses in part through immunosuppressive mechanisms. There are several immune modulatory mechanisms. Among them, adenosine is an important factor which is generated by both cancer and immune cells in tumor microenvironment to suppress anti-tumor responses. Two cell surface expressed ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Kazemi MH,Raoofi Mohseni S,Hojjat-Farsangi M,Anvari E,Ghalamfarsa G,Mohammadi H,Jadidi-Niaragh F

    更新日期:2018-03-01 00:00:00

  • Galactosemia: the good, the bad, and the unknown.

    abstract::Alpha-D-galactose is metabolized in species ranging from E. coli to mammals predominantly via a series of sequential reactions collectively known as the Leloir pathway. Deficiency of any one of these enzymes in humans results in a form of the inherited metabolic disorder, galactosemia, although the symptoms and severi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Fridovich-Keil JL

    更新日期:2006-12-01 00:00:00

  • Extinction of the human insulin gene expression in insulinoma x fibroblast somatic cell hybrids involves cis-acting DNA elements.

    abstract::Insulin gene expression in rat insulinoma (RIN) cells is extinct in RIN x fibroblast hybrids and can reappear upon loss of DNA contributed by the fibroblast parent. (Besnard et al., Exp. Cell Res. 185:101-108, 1989). In the present study, we looked for the role of 5'-flanking sequences of the human insulin gene in the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Besnard C,Monthioux E,Loràs P,Jami J,Daegelen D

    更新日期:1991-03-01 00:00:00

  • Increased transcriptional response to mechanical strain in keloid fibroblasts due to increased focal adhesion complex formation.

    abstract::Clinicians have observed that keloids preferentially form in body areas subject to increased skin tension. We hypothesized a difference exists in the transcriptional response of keloid fibroblasts to mechanical strain compared with normal fibroblasts. Normal and keloid fibroblasts were seeded in a device calibrated to...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang Z,Fong KD,Phan TT,Lim IJ,Longaker MT,Yang GP

    更新日期:2006-02-01 00:00:00

  • Cannabinoid receptor 1 mediates palmitic acid-induced apoptosis via endoplasmic reticulum stress in human renal proximal tubular cells.

    abstract::The endocannabinoid system (ECS) is activated at the onset of obesity and diverse metabolic diseases. Endocannabinoids mediate their physiological and behavioral effects by activating specific cannabinoid receptors, mainly cannabinoid receptor 1 (CB(1)R). Diabetic nephropathy (DN) is induced by hyperlipidemia, and ren...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lim JC,Lim SK,Han HJ,Park SH

    更新日期:2010-11-01 00:00:00

  • Direct effects of wheat germ agglutinin on inositol phosphate formation and cytosolic-free calcium level in intestine 407 cells.

    abstract::The interaction between dietary lectins, especially wheat germ agglutinin (WGA), and intestinal cells has been implicated in the pathogenesis of celiac disease. The present study was undertaken to investigate the immediate effects following such an interaction. Direct WGA-stimulation of Intestine 407 cells leads to an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sjölander A

    更新日期:1988-03-01 00:00:00

  • Comparative genomics of NAD(P) biosynthesis and novel antibiotic drug targets.

    abstract::NAD(P) is an indispensable cofactor for all organisms and its biosynthetic pathways are proposed as promising novel antibiotics targets against pathogens such as Mycobacterium tuberculosis. Six NAD(P) biosynthetic pathways were reconstructed by comparative genomics: de novo pathway (Asp), de novo pathway (Try), NmR pa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Bi J,Wang H,Xie J

    更新日期:2011-02-01 00:00:00

  • Tumor necrosis factor alpha: posttranscriptional stabilization of WAF1 mRNA in p53-deficient human leukemic cells.

    abstract::The p53 protein directly regulates the expression of the WAF1 (wild-type p53-activated fragment 1) protein which is a cyclin-dependent kinase inhibitor (CDK1). DNA damaging agents such as ionizing or UV radiation, and some chemical agents induce WAF1 in wild-type p53 containing cells, thereby halting cell cycle progre...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Shiohara M,Akashi M,Gombart AF,Yang R,Koeffler HP

    更新日期:1996-03-01 00:00:00

  • Temperature dependent expression of polyoma virus in murine embryonal carcinoma cells.

    abstract::At 37 degrees C, undifferentiated murine teratocarcinoma cells (PCC4) are resistant to infection with SV40 and Polyoma virus. When infection is carried out at 31 degrees C, these cells become fully susceptible to a variety of polyoma virus strains, including wt, ts-a, and hr-t; they also display an increased susceptib...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dandolo L,Vasseur M,Kress C,Aghion J,Blangy D

    更新日期:1980-10-01 00:00:00

  • BMP-2 increases migration of human chondrosarcoma cells via PI3K/Akt pathway.

    abstract::Bone morphogenetic protein-2 (BMP-2), a member of transforming growth factor-beta superfamily, plays a crucial role in migration and metastasis of human cancer cells. Integrins are the major adhesive molecules in mammalian cells. Here we found that BMP-2 directed the migration and increased cell surface and mRNA expre...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Fong YC,Li TM,Wu CM,Hsu SF,Kao ST,Chen RJ,Lin CC,Liu SC,Wu CL,Tang CH

    更新日期:2008-12-01 00:00:00

  • Biochemical genetic analysis of pyrimidine biosynthesis in mammalian cells. II. Isolation and characterization of a mutant of Chinese hamster ovary cells with defective dihydroorotate dehydrogenase (E.C. activity.

    abstract::A mutant (A204) of Chinese hamster ovary cells (CHO-K1), which is deficient in dihydroorotate (DHO) dehydrogenase (E.C. 1.3,3.1) activity, has been isolated by a replica plating procedure. The mutant does not show a requirement for exogenously added pyrimidines. Examination of intact cells shows that the mutant accumu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Stamato TD,Patterson D

    更新日期:1979-03-01 00:00:00

  • Functional activity of serotoninergic and melatoninergic systems expressed in the skin.

    abstract::We tested the expression of genes coding receptors of a cutaneous serotoninergic/melatoninergic system in whole human skin and in normal and pathologic cultured skin cells. Evaluation of serotonin (5HT), melatonin (MT), and melatonin-related receptors (MRR) showed expression of the isoforms 5HT2B, 5HT7, and MT1 genes ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Slominski A,Pisarchik A,Zbytek B,Tobin DJ,Kauser S,Wortsman J

    更新日期:2003-07-01 00:00:00

  • G Protein γ subunit 7 loss contributes to progression of clear cell renal cell carcinoma.

    abstract::Clear cell renal cell carcinoma (ccRCC) is a common urinary neoplasm, looking for useful candidates to establish scientific foundation for the therapy of ccRCC is urgent. We downloaded genomic profiles of GSE781, GSE6244, GSE53757, and GSE66271 from the Gene Expression Omnibus (GEO) database. GEO2R was used to analyze...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xu S,Zhang H,Liu T,Chen Y,He D,Li L

    更新日期:2019-11-01 00:00:00

  • Intracellular chloride regulates cell proliferation through the activation of stress-activated protein kinases in MKN28 human gastric cancer cells.

    abstract::Recently, we reported that reduction of intracellular Cl(-) concentration ([Cl(-)](i)) inhibited proliferation of MKN28 gastric cancer cells by diminishing the transition rate from G(1) to S cell-cycle phase through upregulation of p21, cyclin-dependent kinase inhibitor, in a p53-independent manner. However, it is sti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ohsawa R,Miyazaki H,Niisato N,Shiozaki A,Iwasaki Y,Otsuji E,Marunaka Y

    更新日期:2010-06-01 00:00:00

  • The pur protein family: genetic and structural features in development and disease.

    abstract::The Pur proteins are an ancient family of sequence-specific single-stranded nucleic acid-binding proteins. They bind a G-rich element in either single- or double-stranded nucleic acids and are capable of displacing the complementary C-rich strand. Recently several reports have described Pur family member knockouts, mu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Johnson EM,Daniel DC,Gordon J

    更新日期:2013-05-01 00:00:00

  • Insulin-like growth factor-I increases bone sialoprotein (BSP) expression through fibroblast growth factor-2 response element and homeodomain protein-binding site in the proximal promoter of the BSP gene.

    abstract::Insulin-like growth factor-I (IGF-I) promotes bone formation by stimulating proliferation and differentiation of osteoblasts. Bone sialoprotein (BSP), is thought to function in the initial mineralization of bone, is selectively expressed by differentiated osteoblast. To determine the molecular mechanism of IGF-I regul...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Nakayama Y,Nakajima Y,Kato N,Takai H,Kim DS,Arai M,Mezawa M,Araki S,Sodek J,Ogata Y

    更新日期:2006-08-01 00:00:00

  • Decreased in vivo and in vitro colony stimulating activity responses to bacterial lipopolysaccharide in C3H/HeJ mice.

    abstract::Mice of the C3H/HeJ strain exhibit low inflammatory and immunological responses to certain bacterial lipopolysaccharide (LPS) preparations. Lymphocytes from C3H/HeJ mice also show defective LPS-induced mitogenesis. We tested the colony stimulating activity (CSA) response of C3H/HeJ mice. As controls we used mice of th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Russo M,Lutton JD

    更新日期:1977-08-01 00:00:00

  • Proteasome inhibitor MG132 induces BAG3 expression through activation of heat shock factor 1.

    abstract::BAG3 protein, a member of the BAG co-chaperones family, sustains cell survival in a variety of normal and neoplastic cell types, via its interaction with a variety of partners, such as the heat shock protein (HSP) 70, Bcl-2, Raf-1 and others. Expression of BAG3 is induced by some stressful stimuli, such as heat shock,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Du ZX,Zhang HY,Meng X,Gao YY,Zou RL,Liu BQ,Guan Y,Wang HQ

    更新日期:2009-03-01 00:00:00

  • Sevoflurane anesthesia represses neurogenesis of hippocampus neural stem cells via regulating microRNA-183-mediated NR4A2 in newborn rats.

    abstract::Sevoflurane has been commonly utilized in nonobstetric surgeries in pregnant women, and its impacts on fetal brain are still not completely known. Ectopic NR4A2 expression has been reported to be related with familial Parkinson disease, and through dual luciferase we found that NR4A2 is a target gene of microRNA-183 (...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Shao CZ,Xia KP

    更新日期:2019-04-01 00:00:00

  • Modulating calcium-mediated NFATc1 and mitogen-activated protein kinase deactivation underlies the inhibitory effects of kavain on osteoclastogenesis and bone resorption.

    abstract::Osteoclasts are responsible for bone resorption during the process of bone remodeling. Increased osteoclast numbers and bone resorption activity are the main factors contributing to bone loss-related diseases such as osteoporosis. Therefore, modulating the formation and function of osteoclasts is critical for the effe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Guo Q,Cao Z,Wu B,Chen F,Tickner J,Wang Z,Qiu H,Wang C,Chen K,Tan R,Gao Q,Xu J

    更新日期:2018-01-01 00:00:00

  • Peroxisome proliferator-activated receptor (PPAR)gamma is expressed by human spermatozoa: its potential role on the sperm physiology.

    abstract::The peroxisome proliferation-activated receptor gamma (PPARgamma) is mainly expressed in the adipose tissue and integrates the control of energy, lipid, and glucose homeostasis. The present study, by means of RT-PCR, Western blot, and immunofluorescence techniques, demonstrates that human sperm express the PPARgamma. ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Aquila S,Bonofiglio D,Gentile M,Middea E,Gabriele S,Belmonte M,Catalano S,Pellegrino M,Andò S

    更新日期:2006-12-01 00:00:00

  • C-C motif chemokine ligand 23 abolishes ER stress- and LPS-induced reduction in proliferation of bovine endometrial epithelial cells.

    abstract::To reduce embryonic losses in domestic animals for economic production of livestock meat and milk, chemokines and their receptors are required for proper implantation and placentation during early pregnancy. In this study, we investigated the effects of C-C-motif chemokine ligand 23 (CCL23) on the proliferation of bov...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lim W,Bae H,Bazer FW,Song G

    更新日期:2018-04-01 00:00:00

  • Serum-stimulated changes in calcium transport and distribution in mouse 3T3 cells and their modification by dibutyryl cyclic AMP.

    abstract::Serum stimulation of quiescent 3T3 cells returns the cells to a proliferative state. Changes in Ca content, transport and distribution during the transition through G1 and S phase have been investigated following serum stimulation of these cells. 45 Ca exchange data indicate at least two kinetically defined cellular c...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tupper JT,Del Rosso M,Hazelton B,Zorgniotti F

    更新日期:1978-04-01 00:00:00

  • Insulin-like growth factor-binding protein enhancement of insulin-like growth factor-I (IGF-I)-mediated DNA synthesis and IGF-I binding in a human breast carcinoma cell line.

    abstract::The insulin-like growth factors (IGFs) are potent mitogens for malignant cell proliferation. The majority of secreted IGFs are bound to specific IGF-binding proteins (IGFBPs) that are secreted by a large number of cells. These proteins may either inhibit or enhance IGF actions. Breast carcinoma cells secrete a variety...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chen JC,Shao ZM,Sheikh MS,Hussain A,LeRoith D,Roberts CT Jr,Fontana JA

    更新日期:1994-01-01 00:00:00

  • Gap junctions in human synovial cells and tissue.

    abstract::Our objective was to establish the existence of intercellular communication through gap junctions in synovial lining cells and in primary and passaged cultures of human synovial cells. Communication between cells was assessed using the nystatin perforated-patch method, fluorescent dye transfer, immunochemistry, transm...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kolomytkin OV,Marino AA,Sadasivan KK,Meek WD,Wolf RE,Hall V,McCarthy KJ,Albright JA

    更新日期:2000-07-01 00:00:00

  • Differential role of Rho GTPases in intestinal epithelial barrier regulation in vitro.

    abstract::Maintenance of intestinal epithelial barrier functions is crucial to prevent systemic contamination by microbes that penetrate from the gut lumen. GTPases of the Rho-family such as RhoA, Rac1, and Cdc42 are known to be critically involved in the regulation of intestinal epithelial barrier functions. However, it is sti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Schlegel N,Meir M,Spindler V,Germer CT,Waschke J

    更新日期:2011-05-01 00:00:00

  • Coordinated Dynamics of RNA Splicing Speckles in the Nucleus.

    abstract::Despite being densely packed with chromatin, nuclear bodies and a nucleoskeletal network, the nucleus is a remarkably dynamic organelle. Chromatin loops form and relax, RNA transcripts and transcription factors move diffusively, and nuclear bodies move. We show here that RNA splicing speckled domains (splicing speckle...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang Q,Kota KP,Alam SG,Nickerson JA,Dickinson RB,Lele TP

    更新日期:2016-06-01 00:00:00

  • Embryologic origin of endometriosis: analysis of 101 human female fetuses.

    abstract::The etiology of endometriosis, a gynecological disease characterized by the presence of endometrial glands and stroma outside the uterine cavity, is still unknown. Our research group has recently demonstrated the presence of ectopic endometrium in human female fetuses at different gestational ages. In this manuscript ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Signorile PG,Baldi F,Bussani R,Viceconte R,Bulzomi P,D'Armiento M,D'Avino A,Baldi A

    更新日期:2012-04-01 00:00:00