Abstract:
:In this study we determined the effect of reactive oxygen species (ROS) generation during incubation in media at 39 degrees C on ram spermatozoa and the protection by exogenously added antioxidant enzyme, superoxide dismutase (SOD). A novel Cu/Zn-SOD, isolated from the fungal strain Humicola lutea 103 (HLSOD), was used. Our results point out that the levels of both, superoxide anion radicals (*O2-) and H2O2, increase approximately 8-10- and 2-3-fold, respectively, during incubation of spermatozoa. Enhanced ROS generation coincided with reduction of motility, independently of the type of diluted medium. Addition of HLSOD (30, 60 and 120 U ml(-1) sperm) improved sperm functions, maintaining almost initial percentages of motile spermatozoa and increasing the values of mean cytochemical coefficient. At the same time, a significant diminution of *O2- and H2O2 content in the presence of antioxidant enzyme was established. The results suggest that HLSOD is an effective *O2- scavenger in semen that leads to protection of sperm functions.
journal_name
Andrologiajournal_title
Andrologiaauthors
Stefanov R,Angelova M,Stefanova T,Subev M,Dolashka P,Voelter W,Zachariev Zdoi
10.1111/j.1439-0272.2004.00604.xkeywords:
subject
Has Abstractpub_date
2004-04-01 00:00:00pages
51-6issue
2eissn
0303-4569issn
1439-0272pii
AND604journal_volume
36pub_type
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