A gene atlas of the mouse and human protein-encoding transcriptomes.

abstract：:By commandeering cellular translation initiation factors, or destroying those dispensable for viral mRNA translation, viruses often suppress host protein synthesis. In contrast, cellular protein synthesis proceeds in human cytomegalovirus (HCMV)-infected cells, forcing viral and cellular mRNAs to compete for limiting ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McKinney C,Perez C,Mohr I

更新日期：2012-04-10 00:00:00

abstract：:We have developed three computer programs for comparisons of protein and DNA sequences. They can be used to search sequence data bases, evaluate similarity scores, and identify periodic structures based on local sequence similarity. The FASTA program is a more sensitive derivative of the FASTP program, which can be us...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pearson WR,Lipman DJ

更新日期：1988-04-01 00:00:00

abstract：:It is known from a variety of experimental systems that the ability of tumor cells to grow locally and metastasize can be affected by the presence of adjacent normal tissues and cells, particularly mesenchymally derived stromal cells such as fibroblasts. However, the comparative influence of such normal cell-tumor cel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cornil I,Theodorescu D,Man S,Herlyn M,Jambrosic J,Kerbel RS

更新日期：1991-07-15 00:00:00

abstract：:The DNA in chromatin of isolated thymus nuclei, and in two different preparations of deoxyribonucleoprotein extracted from chromatin, has been digested with DNase. By variation of the time of reaction and the concentration of enzyme, and by comparison of the action of the enzyme on (protein-free) DNA with its action o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mirsky AE

更新日期：1971-12-01 00:00:00

abstract：:A major problem facing the effective treatment of patients with cancer is how to get the specific antitumor agent into every tumor cell. In this report we describe the use of a strategy that, by using retroviral vectors encoding a truncated human CD5 cDNA, allows the selection of only the infected cells, and we show t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： García-Hernández B,Castellanos A,López A,Orfao A,Sánchez-García I

更新日期：1997-11-25 00:00:00

abstract：:The mechanism of proton transfer from the bulk into the membrane protein interior was studied. The light-induced reduction of a bound ubiquinone molecule Q(B) by the photosynthetic reaction center is accompanied by proton trapping. We used kinetic spectroscopy to measure (i) the electron transfer to Q(B) (at 450 nm), ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gopta OA,Cherepanov DA,Junge W,Mulkidjanian AY

更新日期：1999-11-09 00:00:00

abstract：:beta 1-Adrenergic receptors from turkey erythrocyte membranes have been identified by specific binding of the radiolabeled antagonist (-)-[3H]dihydroalprenolol. These receptors are inactivated by the alkylating agent N-ethylmaleimide when occupied by beta-adrenergic agonists but not when occupied by antagonists or whe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vauquelin G,Bottari S,Andre C,Jacobsson B,Strosberg AD

更新日期：1980-07-01 00:00:00

abstract：:The two internal transcribed spacers (ITS1 and ITS2) of rDNA of three members of the Ixodes ricinus "complex" (Acari: Ixodidae) were sequenced. Sequence variation was assessed for the North American species I. scapularis, I. dammini, and I. pacificus at three levels: within individual/population, between individuals o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wesson DM,McLain DK,Oliver JH,Piesman J,Collins FH

更新日期：1993-11-01 00:00:00

abstract：:It was reported that the rate of elongation of partially reconstituted RNA, if prepared without centrifugation, showed dependency upon the state of protein aggregation like that found when free RNA was used as the nucleic acid source [Butler, P. J. G. (1974) J. Mol. Biol. 82, 333-341]. Butler concluded from this resul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohno T,Takahashi M,Okada Y

更新日期：1977-02-01 00:00:00

abstract：:In classical evolutionary theory, traits evolve because they facilitate organismal survival and/or reproduction. We discuss a different type of evolutionary mechanism that relies upon differential dispersal. Traits that enhance rates of dispersal inevitably accumulate at expanding range edges, and assortative mating b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shine R,Brown GP,Phillips BL

更新日期：2011-04-05 00:00:00

abstract：:Superhard metals are of interest as possible replacements with enhanced properties over the metal carbides commonly used in cutting, drilling, and wear-resistant tooling. Of the superhard metals, the highest boride of tungsten--often referred to as WB4 and sometimes as W(1-x)B3--is one of the most promising candidates...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lech AT,Turner CL,Mohammadi R,Tolbert SH,Kaner RB

更新日期：2015-03-17 00:00:00

abstract：:Optimization of hydride transfer (HT) catalysts to enhance rates and selectivities of (photo)electroreduction reactions could be a crucial component of a sustainable chemical industry. Here, we analyze how ring functionalization of the adsorbed transient intermediate 2-pyridinide (2-PyH-*)-predicted to form in situ fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu S,Carter EA

更新日期：2019-11-12 00:00:00

abstract：:The completion of the human genome project and the development of high-throughput approaches herald a dramatic acceleration in the pace of biological research. One of the most compelling next steps will be learning the functional roles of all proteins. Achievement of this goal depends in part on the rapid expression a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Braun P,Hu Y,Shen B,Halleck A,Koundinya M,Harlow E,LaBaer J

更新日期：2002-03-05 00:00:00

abstract：:Antibodies have been prepared to the core protein of cartilage chondroitin sulfate proteoglycan from clones isolated after fusion of rat spleen cells and mouse myeloma cell lines. Antibodies produced by five clones were studied in detail by a radioimmunoassay utilizing 35SO42-labeled hyaluronidase-digested chondroitin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dorfman A,Hall T,Ho PL,Fitch F

更新日期：1980-07-01 00:00:00

abstract：:Ocean acidification (OA) refers to the ongoing decline in oceanic pH resulting from the uptake of atmospheric CO(2). Mounting experimental evidence suggests that OA will have negative consequences for a variety of marine organisms. Whereas the effect of OA on the calcification of adult reef corals is increasingly well...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Albright R,Mason B,Miller M,Langdon C

更新日期：2010-11-23 00:00:00

abstract：:Skeletal muscle adapts to different patterns of motor nerve activity by alterations in gene expression that match specialized properties of contraction, metabolism, and muscle mass to changing work demands (muscle plasticity). Calcineurin, a calcium/calmodulin-dependent, serine-threonine protein phosphatase, has been ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosenberg P,Hawkins A,Stiber J,Shelton JM,Hutcheson K,Bassel-Duby R,Shin DM,Yan Z,Williams RS

更新日期：2004-06-22 00:00:00

abstract：:[3H]Forskolin bound to high- and low-affinity sites in the rat brain. The high-affinity site was discretely located, with highest densities in the striatum, nucleus accumbens, olfactory tubercule, substantia nigra, hippocampus, and the molecular layers of the cerebellum. This site did not correlate well with the distr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poat JA,Cripps HE,Iversen LL

更新日期：1988-05-01 00:00:00

abstract：:Rifamycin antibiotics, which inhibit RNA-directed DNA polymerase of Rauscher leuckemia virus, prevent the leukemogenic activity of the virus, and the effect of leukemogenesis correlates with the magnitude of inhibition of the purified enzyme. This inhibition of enzyme activity by rifamycin SV derivatives is due to a r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu AM,Ting RC,Gallo RC

更新日期：1973-05-01 00:00:00

abstract：:The main consequence of the Chernobyl accident has been an increase in papillary thyroid carcinomas (PTCs) in those exposed to radioactive fallout as young children. Our aim was to identify genomic alterations that are associated with exposure to radiation. We used array comparative genomic hybridization to analyze a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hess J,Thomas G,Braselmann H,Bauer V,Bogdanova T,Wienberg J,Zitzelsberger H,Unger K

更新日期：2011-06-07 00:00:00

abstract：:The use of matching variable number of tandem repeat (VNTR) profiles to link suspects with crimes is potentially very powerful, but it has been quite controversial. Initial debate over laboratory procedures has largely given way to debate over the statistical and population genetic issues involved in calculating the f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weir BS

更新日期：1992-12-15 00:00:00

abstract：:We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tao M,Lipmann F

更新日期：1969-05-01 00:00:00

abstract：:Malaria merozoite invasion of human erythrocytes depends on recognition of specific erythrocyte surface receptors by parasite ligands. Plasmodium vivax merozoite invasion is totally dependent on the recognition of the Duffy blood group antigen by the parasite ligand Duffy-binding protein (DBP). Receptor recognition by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： VanBuskirk KM,Sevova E,Adams JH

更新日期：2004-11-02 00:00:00

abstract：:Estrogen receptor alpha (ERalpha) plays a major role in the regulation of neuroendocrine functions and behaviors by estrogens. Although the generation of ERalpha knockout mice advanced our knowledge of ERalpha functions, gene deletion using this method is global and potentially confounded by developmental consequences...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Musatov S,Chen W,Pfaff DW,Kaplitt MG,Ogawa S

更新日期：2006-07-05 00:00:00

abstract：:Most poxviruses, including variola, the causative agent of smallpox, express a secreted protein of 35 kDa, vCCI, which binds CC-chemokines with high affinity. This viral protein competes with the host cellular CC-chemokine receptors (CCRs), reducing inflammation and interfering with the host immune response. Such prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carfí A,Smith CA,Smolak PJ,McGrew J,Wiley DC

更新日期：1999-10-26 00:00:00

abstract：:Many studies have shown that individuals from invasive populations of many different plant species grow larger than individuals from native populations and that this difference has a genetic basis. This increased vigor in invasive populations is thought to be due to life history tradeoffs, in which selection favors th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feng YL,Lei YB,Wang RF,Callaway RM,Valiente-Banuet A,Inderjit,Li YP,Zheng YL

更新日期：2009-02-10 00:00:00

abstract：:Recent studies on proteins whose N and C termini are in close proximity have demonstrated that folding of polypeptide chains and assembly of oligomers can be accomplished with circularly permuted chains. As yet no methodical study has been conducted to determine how extensively new termini can be introduced and where ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graf R,Schachman HK

更新日期：1996-10-15 00:00:00

abstract：:The broad negative consequences of habitat degradation on biodiversity have been studied, but the complex effects of natural-agricultural landscape matrices remain poorly understood. Here we used stable carbon and nitrogen isotopes to detect changes in mammal resource and habitat use and trophic structure between pres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Magioli M,Moreira MZ,Fonseca RCB,Ribeiro MC,Rodrigues MG,Ferraz KMPMB

更新日期：2019-09-10 00:00:00

abstract：:The structure and the expression of 11 cellular oncogenes (protooncogenes) were analyzed in primary cells from 20 acute lymphocytic (ALL) and 31 acute myelogenous (AML) leukemia patients. Neoplastic cells, obtained prior to initiation of therapy, were purified and classified, on the basis of both surface antigen patte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mavilio F,Sposi NM,Petrini M,Bottero L,Marinucci M,De Rossi G,Amadori S,Mandelli F,Peschle C

更新日期：1986-06-01 00:00:00

abstract：:Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation on an autoradiograph, allows more sequence to be obtained from a gel. We also show...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biggin MD,Gibson TJ,Hong GF

更新日期：1983-07-01 00:00:00

abstract：:Clonal deletion provides an important mechanism for the elimination of autoreactive T cells. Deletion is accomplished by programmed cell death directed by interaction of the T-cell receptor (TCR) of the developing thymocyte with major histocompatibility complex elements in the thymic environment. In this report we pre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Russell JH,White CL,Loh DY,Meleedy-Rey P

更新日期：1991-03-15 00:00:00