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Construction of high-density display of CD147 ectodomain on VCSM13 phage via gpVIII: effects of temperature, IPTG, and helper phage infection-period.

Abstract：

:Production of VCSM13 phage displaying a high density of CD147 ectodomain (CD147Ex) was achieved when culturing conditions were modulated. A phagemid expressing CD147Ex was constructed and used to produce phage display CD147Ex gpVIII fusion protein in TG1 Escherichia coli. Displaying of CD147Ex via gpVIII was successfully increased when growing the transformed TG1 at 25 degrees C with IPTG-stimulation. In addition to temperature and IPTG-stimulation, the VCSM13 helper phage infection-period particularly affected the insertion of CD147Ex into phage progeny. By sandwich ELISA, incorporation of the CD147Ex into phage particle was confirmed. The correct size of the CD147Ex-gpVIII fusion protein at 28kDa was demonstrated by Western immunoblotting. Multivalent display of CD147Ex on phage particles will be valuable in discovering its ligand partner.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Intasai N,Arooncharus P,Kasinrerk W,Tayapiwatana C

doi

10.1016/j.pep.2003.08.019

keywords:

subject

Has Abstract

pub_date

2003-12-01 00:00:00

pages

323-31

issue

2

eissn

1046-5928

issn

1096-0279

pii

S1046592803002857

journal_volume

32

pub_type

杂志文章

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