Abstract:
:Inorganic phosphate (Pi) is a physiological regulator of osteoblasts and chondrocytes, suggesting that phosphate may contribute to the biological response of these cells to bioactive glasses like Bioglass 45S5, which is composed of 45% SiO2, 24.5% CaO, 24.5% Na2O, and 6% P2O5. We investigated the effect of varying the Pi content of bioactive glass disks (0%, 3%, 6% and 12% P2O5) using human osteoblast-like MG63 cells as the model. Cell number on 6% Pi disks was comparable to cultures on tissue culture plastic, but was reduced at higher and lower Pi concentrations. Alkaline phosphatase specific activity of isolated cells and cell layer lysates, as well as PGE2, TGF-beta1 and NO levels in conditioned media, were elevated in cultures grown on bioactive glass and varied with the Pi content. The greatest effects were observed in cultures grown on disks with the lowest Pi concentrations. Thus, growth on the bioactive glasses enhances cell function in comparison with tissue culture plastic and lower Pi content favors osteoblast differentiation.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Lossdörfer S,Schwartz Z,Lohmann CH,Greenspan DC,Ranly DM,Boyan BDdoi
10.1016/j.biomaterials.2003.09.094keywords:
subject
Has Abstractpub_date
2004-06-01 00:00:00pages
2547-55issue
13eissn
0142-9612issn
1878-5905pii
S014296120300783Xjournal_volume
25pub_type
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