当前位置： SCI文献检索 > INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE期刊下所有文献 > Vitamin D analogues increase p53, p21, and apoptosis in a xenograft model of human retinoblastoma.

Vitamin D analogues increase p53, p21, and apoptosis in a xenograft model of human retinoblastoma.

Abstract：

PURPOSE:To study the antineoplastic effect of vitamin D analogues in a xenograft model of human retinoblastoma. METHODS:Athymic mice were injected subcutaneously with Y79 cells and treated 5 days a week with either mineral oil (control group) or the vitamin D analogues calcitriol or 1,25-dihydroxy-16-ene-23-yne vitamin D(3) (16,23-D(3)). BrdU was injected 1 hour before death. Animals were killed after 1, 2, 3, or 5 weeks. Paraffin-embedded sections of the tumors were studied for cell proliferation by monitoring for BrdU incorporation and cell death by terminal transferase dUTP-nick end labeling (TUNEL), 3'-overhang ligation, and histology. Sections of the tumors were immunostained for p53 and p21. RESULTS:There was no significant difference in incorporation of BrdU among the three groups, suggesting that cell proliferation is unaffected by vitamin D analogues. TUNEL was increased in tumors treated with vitamin D analogues compared with the control group. This increase was statistically significant for calcitriol in the time frame examined, but not statistically significant for 16,23-D(3). Alternatively, the ratio of proliferation to cell death was significantly different for both calcitriol and 16,23-D(3) compared with control tumors after 3 weeks of treatment. Dying cells contained DNA strand breaks with overhanging nucleotides and nuclear changes characteristic of apoptosis. There was an increase in staining for p53 and p21 in areas associated with cell death in specimens treated with vitamin D analogues. CONCLUSIONS:Vitamin D analogues appear to attenuate retinoblastoma tumor growth in athymic mice by increasing apoptosis. Cell death is associated with the upregulation of both p53 and p21.

journal_name

Invest Ophthalmol Vis Sci

journal_title

Investigative ophthalmology & visual science

authors

Audo I,Darjatmoko SR,Schlamp CL,Lokken JM,Lindstrom MJ,Albert DM,Nickells RW

doi

10.1167/iovs.02-1198

keywords:

subject

Has Abstract

pub_date

2003-10-01 00:00:00

pages

4192-9

issue

10

eissn

0146-0404

issn

1552-5783

journal_volume

44

pub_type

杂志文章

相关文献

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE文献大全
  • Using two preferred retinal loci for different lighting conditions in patients with central scotomas.

    abstract：PURPOSE:Using a scanning laser ophthalmoscope, it was found that some patients with relative central scotomas reliably used two different preferred retinal loci (PRLs) at different stimulus illuminances. This article describes adaptations in a patient's PRL for fixation when dimming the stimulus increased the relative ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Lei H,Schuchard RA

    更新日期：1997-08-01 00:00:00

  • Proliferative diabetic retinopathy with lymphocyte-rich epiretinal membrane associated with poor visual prognosis.

    abstract：PURPOSE:The aim of this study was to analyze lymphocyte infiltration using immunohistochemistry in proliferative diabetic retinopathy (PDR) membranes. METHODS:Sixteen patients, 13 with PDR and 3 without diabetes, underwent pars plana vitrectomy, and the epiretinal membrane was peeled. Formalin-fixed, paraffin-embedded...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.09-3767

    authors： Kase S,Saito W,Ohno S,Ishida S

    更新日期：2009-12-01 00:00:00

  • Fate of orthotopic corneal allografts in eyes that cannot support anterior chamber-associated immune deviation induction.

    abstract：PURPOSE:Corneal allografts placed in human eyes at high risk often fail, and immune rejection is thought to be a major pathogenic factor. To understand the immunologic factors responsible for rejection in this instance, the authors have created "high-risk" eyes in mice by inducing corneal neovascularization. The author...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Sano Y,Ksander BR,Streilein JW

    更新日期：1995-10-01 00:00:00

  • Pupillometer-based objective chromatic perimetry in normal eyes and patients with retinal photoreceptor dystrophies.

    abstract：PURPOSE:To evaluate a novel objective perimetry using multifocal chromatic pupil light reflex in normal participants and patients with photoreceptor dysfunction, and to relate this new technique with subjective dark-adapted chromatic Goldmann perimetry. METHODS:Thirty-two eyes of 17 retinitis pigmentosa (RP) or cone-r...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.12-11127

    authors： Skaat A,Sher I,Kolker A,Elyasiv S,Rosenfeld E,Mhajna M,Melamed S,Belkin M,Rotenstreich Y

    更新日期：2013-04-17 00:00:00

  • Structure-Function Agreement Is Better Than Commonly Thought in Eyes With Early Glaucoma.

    abstract：Purpose:To assess the agreement between structural (optical coherence tomography [OCT]) and functional (visual field [VF]) glaucomatous damage with an automated method and deviation/probability maps, and to compare this method to a metric method. Methods:Wide-field spectral-domain OCT scans, including the disc and mac...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.19-27920

    authors： Hood DC,Tsamis E,Bommakanti NK,Joiner DB,Al-Aswad LL,Blumberg DM,Cioffi GA,Liebmann JM,De Moraes CG

    更新日期：2019-10-01 00:00:00

  • Long posterior ciliary arterial blood flow and systemic blood pressure.

    abstract：:The purpose of the current study was to investigate the relationship between systemic blood pressure (BP) and long posterior ciliary arterial (LPCA) blood flow (BF) in response to autonomic drugs, and to determine the types of receptors involved. LPCA BF was measured in anesthetized rabbits at the retrobulbar region u...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Okubo H,Gherezhiher T,Koss MC

    更新日期：1990-05-01 00:00:00

  • Adult discrimination performance for pediatric acuity test optotypes.

    abstract：PURPOSE:To compare adult discrimination performance on nine pediatric visual acuity tests to determine the consistency of optotype design. METHODS:After their binocular acuity was measured with each test, eight adult observers (mean age, 27 years ± 6.3 SD; three emmetropes and five corrected myopes) were shown isolate...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.10-6391

    authors： Candy TR,Mishoulam SR,Nosofsky RM,Dobson V

    更新日期：2011-06-16 00:00:00

  • Macrophage IL-12p70 signaling prevents HSV-1-induced CNS autoimmunity triggered by autoaggressive CD4+ Tregs.

    abstract：PURPOSE:CD4(+)CD25(+)FoxP3(+) naturally occurring regulatory T cells (Tregs) maintain self-tolerance and function to suppress overly exuberant immune responses. However, it is unclear whether innate immune cells modulate Treg function. Here the authors examined the role of innate immunity in lymphomyeloid homeostasis. ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.10-6536

    authors： Mott KR,Gate D,Zandian M,Allen SJ,Rajasagi NK,van Rooijen N,Chen S,Arditi M,Rouse BT,Flavell RA,Town T,Ghiasi H

    更新日期：2011-04-08 00:00:00

  • Molecular characterization of the skate peripherin/rds gene: relationship to its orthologues and paralogues.

    abstract：PURPOSE:A great deal of information about functionally significant domains of a protein may be obtained by comparison of primary sequences of gene homologues over a broad phylogenetic base. This study was designed to identify evolutionarily conserved domains of the photoreceptor disc membrane protein peripherin/rds by ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.02-1152

    authors： Li C,Ding XQ,O'Brien J,Al-Ubaidi MR,Naash MI

    更新日期：2003-06-01 00:00:00

  • Imaging Laser-Induced Choroidal Neovascularization in the Rodent Retina Using Optical Coherence Tomography Angiography.

    abstract：PURPOSE:The purpose of this study was to evaluate the performance of optical coherence tomography angiography (OCTA) in visualizing laser-induced choroidal neovascularization (CNV) in the rodent retina. METHODS:Choroidal neovascularization was induced via laser photocoagulation in 2 male Brown Norway rats and 2 male C...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.15-18946

    authors： Park JR,Choi W,Hong HK,Kim Y,Jun Park S,Hwang Y,Kim P,Joon Woo S,Hyung Park K,Oh WY

    更新日期：2016-07-01 00:00:00

  • Absence of degenerative changes in retinal and uveal capillary pericytes in diabetic rats.

    abstract：:Ultrastructural morphometric techniques were used to quantify pericyte degeneration in retinal and uveal capillaries of streptozotocin-diabetic rats in order to assess the suitability of this small rodent model of diabetes for studies of the pathogenesis of microvascular eye disease in diabetic humans. Male, Sprague-D...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Tilton RG,LaRose LS,Kilo C,Williamson JR

    更新日期：1986-05-01 00:00:00

  • Glaucoma progression in eyes with a history of refractive corneal surgery.

    abstract：PURPOSE:To evaluate the glaucoma progression in patients with a history of refractive corneal surgery (RCS). METHODS:A total of 313 eyes of 313 glaucoma patients (RCS group, 34 eyes; non-RCS group, 279 eyes) with a mean follow-up period of 2.7 years were included. Glaucoma progression was determined either by experts'...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.12-9862

    authors： Kim YJ,Yun SC,Na JH,Tchah HW,Jung JJ,Sung KR

    更新日期：2012-07-03 00:00:00

  • Retinal vessel tortuosity associated with central retinal vein occlusion: an optical coherence tomography study.

    abstract：PURPOSE:We studied morphologic changes of the retinal vasculature in eyes with central retinal vein occlusion (CRVO) through the use of optical coherence tomography (OCT). METHODS:Major retinal vessels in 35 eyes from 35 consecutive patients with acute CRVO were examined prospectively and longitudinally with sequentia...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.13-13201

    authors： Muraoka Y,Tsujikawa A,Kumagai K,Akagi-Kurashige Y,Ogino K,Murakami T,Miyamoto K,Yoshimura N

    更新日期：2014-01-07 00:00:00

  • Blood pressure, perfusion pressure, and open-angle glaucoma: the Los Angeles Latino Eye Study.

    abstract：PURPOSE:To examine the cross-sectional relationship between blood pressure, perfusion pressure, and prevalence of open angle glaucoma (OAG) in an adult Latino population. METHODS:Participants aged 40 years and older (N = 6130) from the Los Angeles Latino Eye Study (LALES), a large, population-based study of self-ident...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.08-2956

    authors： Memarzadeh F,Ying-Lai M,Chung J,Azen SP,Varma R,Los Angeles Latino Eye Study Group.

    更新日期：2010-06-01 00:00:00

  • Clinical assessment of mirror artifacts in spectral-domain optical coherence tomography.

    abstract：:PURPOSE. To investigate the characteristics of a spectral-domain optical coherence tomography (SD-OCT) image phenomenon known as the mirror artifact, calculate its prevalence, analyze potential risk factors, measure severity, and correlate it to spherical equivalent and central visual acuity (VA). METHODS. OCT macular...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.09-4057

    authors： Ho J,Castro DP,Castro LC,Chen Y,Liu J,Mattox C,Krishnan C,Fujimoto JG,Schuman JS,Duker JS

    更新日期：2010-07-01 00:00:00

  • Rapid automated diagnosis of diabetic peripheral neuropathy with in vivo corneal confocal microscopy.

    abstract：PURPOSE:To assess the diagnostic validity of a fully automated image analysis algorithm of in vivo confocal microscopy images in quantifying corneal subbasal nerves to diagnose diabetic neuropathy. METHODS:One hundred eighty-six patients with type 1 and type 2 diabetes mellitus (T1/T2DM) and 55 age-matched controls un...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.13-13787

    authors： Petropoulos IN,Alam U,Fadavi H,Marshall A,Asghar O,Dabbah MA,Chen X,Graham J,Ponirakis G,Boulton AJ,Tavakoli M,Malik RA

    更新日期：2014-04-03 00:00:00

  • Maturation of cyclopean visual evoked potential phase in preterm and full-term infants.

    abstract：PURPOSE:P1 is the major positive component of pattern-reversal visual evoked potentials (PR-VEPs). The rapid decrease of its latency correlates with the progressive myelination in the developing infant brain, which affects signal transmission in the visual system. An age-dependent phase shift, analogous to P1 peak late...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.14-13906

    authors： Mikó-Baráth E,Markó K,Budai A,Török B,Kovacs I,Jandó G

    更新日期：2014-04-21 00:00:00

  • Expression of nerve growth factor receptors on the ocular surface in healthy subjects and during manifestation of inflammatory diseases.

    abstract：PURPOSE:Recent studies have suggested the involvement of nerve growth factor (NGF) in the conjunctival inflammatory process and in corneal epithelium proliferation and differentiation. To verify the hypothesis that NGF could locally modulate the inflammatory and reparative processes, the authors evaluated the expressio...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Lambiase A,Bonini S,Micera A,Rama P,Bonini S,Aloe L

    更新日期：1998-06-01 00:00:00

  • Cone differentiation with no photopigment coexpression.

    abstract：PURPOSE:To decide whether the transitory coexpression of cone visual pigments described in the developing rat and gerbil retina is a universal feature of dichromatic mammalian species. METHODS:The rabbit, a species widely used in eye research, was selected for the study and a search made for the presence of cones that...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Szepessy Z,Lukáts A,Fekete T,Barsi A,Röhlich P,Szél A

    更新日期：2000-09-01 00:00:00

  • Retinoids and butyrate modulate fibroblast growth and contraction of collagen matrices.

    abstract：:Wound healing in the eye may lead to undesirable sequelae such as proliferative vitreoretinopathy and scarring of glaucoma filtering fistulas. We therefore sought to manipulate in vitro two key wound healing processes--cell proliferation and contraction of extracellular matrices--using vitamin A (VA), retinoic acid (R...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Kim RY,Stern WH

    更新日期：1990-06-01 00:00:00

  • Functional and Anatomical Outcomes in Patients With Serous Retinal Detachment in Diabetic Macular Edema Treated With Ranibizumab.

    abstract：Purpose:To assess the effect of serous retinal detachment (SRD) on functional and anatomical outcomes in ranibizumab-treated patients with diabetic macular edema (DME). Methods:All consecutive ranibizumab-treated patients with SRD were included in this retrospective study. For each patient with SRD, a patient without ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-20855

    authors： Giocanti-Aurégan A,Hrarat L,Qu LM,Sarda V,Boubaya M,Levy V,Chaine G,Fajnkuchen F

    更新日期：2017-02-01 00:00:00

  • Prenatal ethanol exposure alters scotopic and photopic components of adult rat electroretinograms.

    abstract：:Ocular malformations and visual deficits are pathognomic of fetal alcohol syndrome (FAS). However, there are no reports on retinal visual function. To determine if prenatal (human second-trimester equivalent) or prenatal plus 10 days of postnatal (human third-trimester equivalent) ethanol exposure produced scotopic an...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Katz LM,Fox DA

    更新日期：1991-10-01 00:00:00

  • Spectrum of Variants in 389 Chinese Probands With Familial Exudative Vitreoretinopathy.

    abstract：Purpose:To identify potentially pathogenic variants (PPVs) in Chinese familial exudative vitreoretinopathy (FEVR) patients in FZD4, LRP5, NDP, TSPAN12, ZNF408, and KIF11 genes. Methods:Blood samples were collected from probands and their parent(s). Genomic DNA was analyzed by next-generation sequencing, and the sequen...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.17-23541

    authors： Li JK,Li Y,Zhang X,Chen CL,Rao YQ,Fei P,Zhang Q,Zhao P,Li J

    更新日期：2018-11-01 00:00:00

  • Macular function impairment in eyes with early age-related macular degeneration.

    abstract：PURPOSE:To study different aspects of macular function in eyes with early age-related macular degeneration (early AMD: drusen with or without retinal pigment epithelium alterations) and normal visual acuity, to obtain a complete evaluation of macular function impairment in early AMD and to study the relationship betwee...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Midena E,Degli Angeli C,Blarzino MC,Valenti M,Segato T

    更新日期：1997-02-01 00:00:00

  • Prevention of ornithine cytotoxicity by nonpolar side chain amino acids in retinal pigment epithelial cells.

    abstract：PURPOSE:To investigate the effect of amino acids on ornithine cytotoxicity in ornithine-delta-aminotransferase (OAT)-deficient human retinal pigment epithelial (RPE) cells as an in vitro model of gyrate atrophy (GA) of the choroid and retina. METHODS:RPE cells were treated with 0.5 mM 5-fluoromethylornithine (5-FMOrn)...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.03-0403

    authors： Nakauchi T,Ando A,Ueda-Yamada M,Yamazaki Y,Uyama M,Matsumura M,Ito S

    更新日期：2003-11-01 00:00:00

  • Repeatability and reproducibility of corneal thickness measurements by optical coherence tomography.

    abstract：PURPOSE:To assess the repeatability and interoperator and intersession reproducibility of central corneal thickness (CCT) measurements made by a commercially available optical coherence tomography (OCT) system. METHODS:Intersession and interoperator reproducibility and repeatability were defined according to the guide...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Muscat S,McKay N,Parks S,Kemp E,Keating D

    更新日期：2002-06-01 00:00:00

  • Relationship between retinal vascular geometry with retinal nerve fiber layer and ganglion cell-inner plexiform layer in nonglaucomatous eyes.

    abstract：PURPOSE:To examine the relationship between retinal vascular geometric parameters with retinal nerve fiber layer (RNFL) and ganglion cell-inner plexiform layer (GC-IPL) parameters in nonglaucomatous subjects. METHODS:Study subjects were identified from the Singapore Chinese Eye Study (SCES), a population-based survey ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.13-12796

    authors： Tham YC,Cheng CY,Zheng Y,Aung T,Wong TY,Cheung CY

    更新日期：2013-11-05 00:00:00

  • Genetic evidence for role of carotenoids in age-related macular degeneration in the Carotenoids in Age-Related Eye Disease Study (CAREDS).

    abstract：PURPOSE:We tested variants in genes related to lutein and zeaxanthin status for association with age-related macular degeneration (AMD) in the Carotenoids in Age-Related Eye Disease Study (CAREDS). METHODS:Of 2005 CAREDS participants, 1663 were graded for AMD from fundus photography and genotyped for 424 single nucleo...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,多中心研究

    doi：10.1167/iovs.13-13216

    authors： Meyers KJ,Mares JA,Igo RP Jr,Truitt B,Liu Z,Millen AE,Klein M,Johnson EJ,Engelman CD,Karki CK,Blodi B,Gehrs K,Tinker L,Wallace R,Robinson J,LeBlanc ES,Sarto G,Bernstein PS,SanGiovanni JP,Iyengar SK

    更新日期：2014-01-29 00:00:00

  • Relationship between retinal layer thickness and the visual field in early age-related macular degeneration.

    abstract：PURPOSE:To quantify and compare the structural and functional changes in subjects with early age-related macular degeneration (AMD), using spectral-domain optical coherence tomography (SD-OCT) and microperimetry. METHODS:Twenty-one eyes of 21 subjects with early AMD were examined. MP-1 10-2 visual fields (VFs) and SD-...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.12-10361

    authors： Acton JH,Smith RT,Hood DC,Greenstein VC

    更新日期：2012-11-09 00:00:00

  • The regional distribution of vitamins E and C in mature and premature human retinas.

    abstract：:Vitamin E is used to ameliorate retinopathy of prematurity, but little is known about baseline vitamin E levels in retinas of premature infants or the effect of vitamin E supplementation on these levels. Vitamin E and C levels were measured in mature retinas (1 month to 73 years) and in retinas of premature infants (2...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Nielsen JC,Naash MI,Anderson RE

    更新日期：1988-01-01 00:00:00