Complementation of a red-light-indifferent cyanobacterial mutant.

Abstract:

:Many cyanobacteria alter their phycobilisome composition in response to changes in light wavelength in a process termed complementary chromatic adaptation. Mutant strains FdR1 and FdR2 of the filamentous cyanobacterium Fremyella diplosiphon are characterized by aberrant chromatic adaptation. Instead of adjusting to different wavelengths of light, FdR1 and FdR2 behave as if they are always in green light; they do not respond to red light. We have previously reported complementation of FdR1 by conjugal transfer of a wild-type genomic library. The complementing DNA has now been localized by genetic analysis to a region on the rescued genomic subclone that contains a gene designated rcaC. This region of DNA is also able to complement FdR2. Southern blot analysis of genomic DNA from FdR1 and FdR2 indicates that these strains harbor DNA insertions within the rcaC sequence that may have resulted from the activity of transposable genetic elements. The predicted amino acid sequence of RcaC shares strong identity to response regulators of bacterial two-component regulatory systems. This relationship is discussed in the context of the signal-transduction pathway mediating regulation of genes encoding phycobilisome polypeptides during chromatic adaptation.

authors

Chiang GG,Schaefer MR,Grossman AR

doi

10.1073/pnas.89.20.9415

keywords:

subject

Has Abstract

pub_date

1992-10-15 00:00:00

pages

9415-9

issue

20

eissn

0027-8424

issn

1091-6490

journal_volume

89

pub_type

杂志文章
  • Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls.

    abstract::To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombin...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0604632103

    authors: Bauer S,Vasu P,Persson S,Mort AJ,Somerville CR

    更新日期:2006-07-25 00:00:00

  • Organization of kappa light chain genes in germ-line and somatic tissue.

    abstract::We studied the organization of the kappa light chain genes in germ-line (sperm) and somatic (embryo) tissues. We constructed a plasmid containing a DNA insert coding for the kappa chain MOPC 167 and used the Southern blotting technique to determine the organization of kappa variable and constant region genes. In the h...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.2.1106

    authors: Joho R,Weissman IL,Early P,Cole J,Hood L

    更新日期:1980-02-01 00:00:00

  • Envelope glycans of immunodeficiency virions are almost entirely oligomannose antigens.

    abstract::The envelope spike of HIV is one of the most highly N-glycosylated structures found in nature. However, despite extensive research revealing essential functional roles in infection and immune evasion, the chemical structures of the glycans on the native viral envelope glycoprotein gp120--as opposed to recombinantly ge...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1006498107

    authors: Doores KJ,Bonomelli C,Harvey DJ,Vasiljevic S,Dwek RA,Burton DR,Crispin M,Scanlan CN

    更新日期:2010-08-03 00:00:00

  • FK506-binding protein (FKBP) partitions a modified HIV protease inhibitor into blood cells and prolongs its lifetime in vivo.

    abstract::HIV protease inhibitors are a key component of anti-retroviral therapy, but their susceptibility to cytochrome P(450) metabolism reduces their systemic availability and necessitates repetitive dosing. Importantly, failure to maintain adequate inhibitor levels is believed to provide an opportunity for resistance to eme...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0805375106

    authors: Marinec PS,Chen L,Barr KJ,Mutz MW,Crabtree GR,Gestwicki JE

    更新日期:2009-02-03 00:00:00

  • Formation of unconventional standing waves at graphene edges by valley mixing and pseudospin rotation.

    abstract::We investigate the roles of the pseudospin and the valley degeneracy in electron scattering at graphene edges. It is found that they are strongly correlated with charge density modulations of short-wavelength oscillations and slowly decaying beat patterns in the electronic density profile. Theoretical analyses using n...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1114548108

    authors: Park C,Yang H,Mayne AJ,Dujardin G,Seo S,Kuk Y,Ihm J,Kim G

    更新日期:2011-11-15 00:00:00

  • Purification and initial characterization of a type beta transforming growth factor from human placenta.

    abstract::A polypeptide transforming growth factor (TGF) that induces anchorage-dependent rat kidney fibroblasts to grow in soft agar has been isolated from human placenta and purified to homogeneity. This polypeptide is classified as a type beta TGF because it does not compete with epidermal growth factor (EGF) for membrane re...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.80.12.3676

    authors: Frolik CA,Dart LL,Meyers CA,Smith DM,Sporn MB

    更新日期:1983-06-01 00:00:00

  • Physical limits to spatial resolution of optical recording: clarifying the spatial structure of cortical hypercolumns.

    abstract::Neurons in macaque primary visual cortex are spatially arranged by their global topographic position and in at least three overlapping local modular systems: ocular dominance columns, orientation pinwheels, and cytochrome oxidase (CO) blobs. Individual neurons in the blobs are not tuned to orientation, and populations...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0500291102

    authors: Polimeni JR,Granquist-Fraser D,Wood RJ,Schwartz EL

    更新日期:2005-03-15 00:00:00

  • HLA class I and II antigens are partially co-clustered in the plasma membrane of human lymphoblastoid cells.

    abstract::Major histocompatibility complex (MHC) class II molecules displayed clustered patterns at the surfaces of T (HUT-102B2) and B (JY) lymphoma cells characterized by interreceptor distances in the micrometer range as detected by scanning force microscopy of immunogold-labeled antigens. Electron microscopy revealed that a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.14.7269

    authors: Jenei A,Varga S,Bene L,Mátyus L,Bodnár A,Bacsó Z,Pieri C,Gáspár R Jr,Farkas T,Damjanovich S

    更新日期:1997-07-08 00:00:00

  • Control of the mRNA for hepatic tryptophan oxygenase during hormonal and substrate induction.

    abstract::Glucocorticoid hormones increase the level of hepatic tryptophan oxygenase (EC 1.13.11.11; L-tryptophan:oxygen 2,3-oxidoreductase (decyclizing) by increasing its rate of synthesis. Studies were performed to determine whether this induction is mediated by controlling the level of the mRNA for tryptophan oxygenase of by...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.3.1017

    authors: Schutz G,Killewich L,Chen G,Feigelson P

    更新日期:1975-03-01 00:00:00

  • Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis.

    abstract::The N-terminal 392 amino acids of the Drosophila kinesin alpha subunit (designated DKH392) form a dimer in solution that releases only one of its two tightly bound ADP molecules on association with a microtubule, whereas a shorter monomeric construct (designated DKH340) releases > or = 95% of its one bound ADP on asso...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.15.6865

    authors: Hackney DD

    更新日期:1994-07-19 00:00:00

  • Cable bacteria generate a firewall against euxinia in seasonally hypoxic basins.

    abstract::Seasonal oxygen depletion (hypoxia) in coastal bottom waters can lead to the release and persistence of free sulfide (euxinia), which is highly detrimental to marine life. Although coastal hypoxia is relatively common, reports of euxinia are less frequent, which suggests that certain environmental controls can delay t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1510152112

    authors: Seitaj D,Schauer R,Sulu-Gambari F,Hidalgo-Martinez S,Malkin SY,Burdorf LD,Slomp CP,Meysman FJ

    更新日期:2015-10-27 00:00:00

  • From the Cover: Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering.

    abstract::With the completion of genome sequences of major model organisms, increasingly sophisticated genetic tools are necessary for investigating the complex and coordinated functions of genes. Here we describe a genetic manipulation system termed "genomic engineering" in Drosophila. Genomic engineering is a 2-step process t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0900641106

    authors: Huang J,Zhou W,Dong W,Watson AM,Hong Y

    更新日期:2009-05-19 00:00:00

  • Hypoxia-activated cytotoxic agent tirapazamine enhances hepatic artery ligation-induced killing of liver tumor in HBx transgenic mice.

    abstract::Transarterial chemoembolization (TACE) is the main treatment for intermediate stage hepatocellular carcinoma (HCC) with Barcelona Clinic Liver Cancer classification because of its exclusive arterial blood supply. Although TACE achieves substantial necrosis of the tumor, complete tumor necrosis is uncommon, and the res...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1613466113

    authors: Lin WH,Yeh SH,Yeh KH,Chen KW,Cheng YW,Su TH,Jao P,Ni LC,Chen PJ,Chen DS

    更新日期:2016-10-18 00:00:00

  • Noninvasive wearable electroactive pharmaceutical monitoring for personalized therapeutics.

    abstract::To achieve the mission of personalized medicine, centering on delivering the right drug to the right patient at the right dose, therapeutic drug monitoring solutions are necessary. In that regard, wearable biosensing technologies, capable of tracking drug pharmacokinetics in noninvasively retrievable biofluids (e.g., ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2009979117

    authors: Lin S,Yu W,Wang B,Zhao Y,En K,Zhu J,Cheng X,Zhou C,Lin H,Wang Z,Hojaiji H,Yeung C,Milla C,Davis RW,Emaminejad S

    更新日期:2020-08-11 00:00:00

  • Targeting cancer micrometastases with monoclonal antibodies: a binding-site barrier.

    abstract::Monoclonal antibodies penetrate bulky tumors poorly after intravenous administration, in part because of specific binding to the target antigen. Experiments presented here demonstrate an analogous phenomenon in micrometastases; poor antibody penetration, attributable to a "binding-site barrier" phenomenon, can be seen...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.19.8999

    authors: Saga T,Neumann RD,Heya T,Sato J,Kinuya S,Le N,Paik CH,Weinstein JN

    更新日期:1995-09-12 00:00:00

  • An active biopolymer network controlled by molecular motors.

    abstract::We describe an active polymer network in which processive molecular motors control network elasticity. This system consists of actin filaments cross-linked by filamin A (FLNa) and contracted by bipolar filaments of muscle myosin II. The myosin motors stiffen the network by more than two orders of magnitude by pulling ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0903974106

    authors: Koenderink GH,Dogic Z,Nakamura F,Bendix PM,MacKintosh FC,Hartwig JH,Stossel TP,Weitz DA

    更新日期:2009-09-08 00:00:00

  • Cell-specific deletion of glucosylceramide synthase in brain leads to severe neural defects after birth.

    abstract::Sialic acid-containing glycosphingolipids, i.e., gangliosides, constitute a major component of neuronal cells and are thought to be essential for brain function. UDP-glucose:ceramide glucosyltransferase (Ugcg) catalyzes the initial step of glycosphingolipid (GSL) biosynthesis. To gain insight into the role of GSLs in ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0500893102

    authors: Jennemann R,Sandhoff R,Wang S,Kiss E,Gretz N,Zuliani C,Martin-Villalba A,Jäger R,Schorle H,Kenzelmann M,Bonrouhi M,Wiegandt H,Gröne HJ

    更新日期:2005-08-30 00:00:00

  • Slowing down of North Pacific climate variability and its implications for abrupt ecosystem change.

    abstract::Marine ecosystems are sensitive to stochastic environmental variability, with higher-amplitude, lower-frequency--i.e., "redder"--variability posing a greater threat of triggering large ecosystem changes. Here we show that fluctuations in the Pacific Decadal Oscillation (PDO) index have slowed down markedly over the ob...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1501781112

    authors: Boulton CA,Lenton TM

    更新日期:2015-09-15 00:00:00

  • Reconstitution of paired T cell receptor alpha- and beta-chains from microdissected single cells of human inflammatory tissues.

    abstract::We describe a strategy to "revive" putatively pathogenic T cells from frozen specimens of human inflammatory target organs. To distinguish pathogenic from irrelevant bystander T cells, we focused on cells that were (i) clonally expanded and (ii) in direct morphological contact with a target cell. Using CDR3 spectratyp...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0604247103

    authors: Seitz S,Schneider CK,Malotka J,Nong X,Engel AG,Wekerle H,Hohlfeld R,Dornmair K

    更新日期:2006-08-08 00:00:00

  • Molecular cloning, nucleotide sequence, and expression of the gene encoding human eosinophil differentiation factor (interleukin 5).

    abstract::The human eosinophil differentiation factor (EDF) gene was cloned from a genomic library in lambda phage EMBL3A by using a murine EDF cDNA clone as a probe. The DNA sequence of a 3.2-kilobase BamHI fragment spanning the gene was determined. The gene contains three introns. The predicted amino acid sequence of 134 amin...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.19.6629

    authors: Campbell HD,Tucker WQ,Hort Y,Martinson ME,Mayo G,Clutterbuck EJ,Sanderson CJ,Young IG

    更新日期:1987-10-01 00:00:00

  • Transcriptional regulation of the Xlim-1 gene by activin is mediated by an element in intron I.

    abstract::The Xlim-1 gene is activated in the late blastula stage of Xenopus embryogenesis in the mesoderm, and its RNA product becomes concentrated in the Spemann organizer at early gastrula stage. A major regulator of early expression of Xlim-1 is activin or an activin-like signal. We report experiments aiming to identify the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.18.9717

    authors: Rebbert ML,Dawid IB

    更新日期:1997-09-02 00:00:00

  • Neoplastic transformation and tumorigenesis by the human protooncogene MYC.

    abstract::Damage to the protooncogene MYC has been implicated in the genesis of diverse human tumors, but the tumorigenic potential of the isolated gene has been disputed. Here we report the use of a retroviral vector to test the potency of human MYC for neoplastic transformation in avian cells. We found that sustained and abun...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.6.2102

    authors: Ramsay GM,Moscovici G,Moscovici C,Bishop JM

    更新日期:1990-03-01 00:00:00

  • Time-resolved x-ray diffraction reveals multiple conformations in the M-N transition of the bacteriorhodopsin photocycle.

    abstract::We measured the M-N transition of wild-type bacteriorhodopsin (pH 9, 10 degrees C) by time-resolved x-ray diffraction study at SPring8 BL45XU-A. We confirmed the accumulation of M and N intermediates by absorbance measurements, and we found that the time resolution of x-ray diffraction experiments (244 ms) was suffici...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.260504897

    authors: Oka T,Yagi N,Fujisawa T,Kamikubo H,Tokunaga F,Kataoka M

    更新日期:2000-12-19 00:00:00

  • Subnuclear relocalization and silencing of a chromosomal region by an ectopic ribosomal DNA repeat.

    abstract::Our research addresses the relationship between subnuclear localization and gene expression in fission yeast. We observed the relocalization of a heterochromatic region, the mating-type region, from its natural location at the spindle-pole body to the immediate vicinity of the nucleolus. Relocalization occurred in res...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1315581110

    authors: Jakociunas T,Domange Jordö M,Aït Mebarek M,Bünner CM,Verhein-Hansen J,Oddershede LB,Thon G

    更新日期:2013-11-19 00:00:00

  • Mitotic Golgi disassembly is required for bipolar spindle formation and mitotic progression.

    abstract::During mitosis, the mammalian Golgi vesiculates and, upon partitioning, reassembles in each daughter cell; however, it is not clear whether the disassembly process per se is important for partitioning or is merely an outcome of mitotic entry. Here, we show that Golgi vesiculation is required for progression to metapha...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1610844113

    authors: Guizzunti G,Seemann J

    更新日期:2016-10-25 00:00:00

  • Targeted manipulation of maize genes in vivo using chimeric RNA/DNA oligonucleotides.

    abstract::Site-specific heritable mutations in maize genes were engineered by introducing chimeric RNA/DNA oligonucleotides. Two independent targets within the endogenous maize acetohydroxyacid synthase gene sequence were modified in a site-specific fashion, thereby conferring resistance to either imidazolinone or sulfonylurea ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.15.8768

    authors: Zhu T,Peterson DJ,Tagliani L,St Clair G,Baszczynski CL,Bowen B

    更新日期:1999-07-20 00:00:00

  • Calcium influx mediated by the Escherichia coli heat-stable enterotoxin B (STB).

    abstract::The heat-stable enterotoxin B (STB) of Escherichia coli is a 48-amino acid extracellular peptide that induces rapid fluid accumulation in animal intestinal models. Unlike other E. coli enterotoxins that elicit cAMP or cGMP responses in the gut [heat-labile toxin (LT) and heat-stable toxin A (STA), respectively], STB i...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.8.3202

    authors: Dreyfus LA,Harville B,Howard DE,Shaban R,Beatty DM,Morris SJ

    更新日期:1993-04-15 00:00:00

  • 3-Hydroxyanthranilic acid inhibits PDK1 activation and suppresses experimental asthma by inducing T cell apoptosis.

    abstract::3-Hydroxyanthranilic acid (HAA), a compound generated during tryptophan metabolism initiated by indoleamine 2,3-dioxygenase, is known to induce T cell death, but its molecular target is not known. Here we report that HAA inhibits NF-kappaB activation upon T cell antigen receptor engagement by specifically targeting PD...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0709261104

    authors: Hayashi T,Mo JH,Gong X,Rossetto C,Jang A,Beck L,Elliott GI,Kufareva I,Abagyan R,Broide DH,Lee J,Raz E

    更新日期:2007-11-20 00:00:00

  • Binding and transcription of relaxed DNA templates by fractions of maize chloroplast extracts.

    abstract::Preparations of partially purified chloroplast DNA-dependent RNA polymerase from maize and some other plants transcribe cloned chloroplast genes preferentially and much more actively from appropriately negatively supercoiled templates than from relaxed templates. We have found that the polymerase in such fractions doe...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.3.876

    authors: Zaitlin D,Hu J,Bogorad L

    更新日期:1989-02-01 00:00:00

  • A TNFSF15 disease-risk polymorphism increases pattern-recognition receptor-induced signaling through caspase-8-induced IL-1.

    abstract::Inflammatory diseases are characterized by dysregulated cytokine production. Altered functions for most risk loci, including the inflammatory bowel disease and leprosy-associated tumor necrosis factor ligand superfamily member 15 (TNFSF15) region, are unclear. Regulation of pattern-recognition-receptor (PRR)-induced s...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1404178111

    authors: Hedl M,Abraham C

    更新日期:2014-09-16 00:00:00