Abstract：
:Human diploid lung fibroblasts (IMR-90) were used to investigate the reported increase in beta-adrenergic-stimulated cyclic adenosine 3',5'-monophosphate (cAMP) levels in fibroblasts aged in culture. Under basal conditions cellular cAMP was 34.2 +/- 5.6 and 38.4 +/- 9.1 pmol/mg protein in early (PDL 22-24) and late (PDL 47-52) passage fibroblasts, respectively. Net release of cAMP from fibroblasts was 67.8 +/- 8.6 and 18.5 +/- 7.0 pmol/30 min/mg protein in early and late passage cultures, respectively. In confluent, early passage fibroblasts, cellular cAMP and net release of cAMP increased by 2.7-fold and 3.8-fold, respectively, after a 30 min incubation in 2 microM isoproterenol. In confluent late passage fibroblasts, isoproterenol incubation increased cellular cAMP and net release of cAMP by 7.8-fold and 26.1-fold, respectively. Adenosine failed to inhibit isoproterenol-induced stimulation of cAMP in early or late passage fibroblasts. There was no passage-related difference in basal, isoproterenol, or forskolin-stimulated adenylyl cyclase activity in crude fibroblast membrane preparations. The activity of cAMP-phosphodiesterase in sonicates of early and late passage IMR-90 was 9.61 +/- 1.15 and 5.81 +/- 1.11 pmol/min/mg protein respectively. Measurements of cAMP in subconfluent early passage fibroblasts indicated that mechanisms related to the reduced cell density in confluent late passage IMR-90 may, in part, account for the enhanced isoproterenol-induced cAMP levels observed in these cultures. The results suggest that the remainder of the enhanced cAMP response to isoproterenol of in vitro aged fibroblasts may be due to a lower cAMP phosphodiesterase activity in these cells.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Ethier MF,Medeiros M,Romano FD,Dobson JG Jrdoi
10.1016/0531-5565(92)90056-6keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
287-300issue
3eissn
0531-5565issn
1873-6815pii
0531-5565(92)90056-6journal_volume
27pub_type
杂志文章abstract：:Women who seek medical care around the time of menopause frequently report changes in one or more aspects of psychological functioning. The etiology of these symptoms is likely multifactorial and, undoubtedly, individual and sociocultural factors are important determinants. The known psychotropic properties of the sex...
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pub_type： 杂志文章,评审
doi：10.1016/0531-5565(94)90021-3
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abstract：:Even though Alzheimer's disease (AD) is the most common cause of dementia, the mechanisms governing the establishment and progression of the disease remain largely unknown. Here, we investigated the implication of the neuroprotective protein BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) in AD and the possibility...
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abstract：:According to the free radical theory of aging, free radicals are involved in the production of changes in cellular metabolism that lead to a time-dependent functional decline in all living beings. Consequently, antioxidant and/or free radicals scavengers may retard the aging process. We explored the effect of melatoni...
journal_title：Experimental gerontology
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journal_title：Experimental gerontology
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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journal_title：Experimental gerontology
pub_type： 杂志文章,随机对照试验
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journal_title：Experimental gerontology
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doi：10.1016/j.exger.2003.12.014
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journal_title：Experimental gerontology
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doi：10.1016/s0531-5565(03)00111-6
更新日期：2003-07-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2016.01.009
更新日期：2016-04-01 00:00:00
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doi：10.1016/0531-5565(95)02027-6
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2015.11.001
更新日期：2015-12-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2014.12.001
更新日期：2015-01-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2004.07.002
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2005.02.005
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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journal_title：Experimental gerontology
pub_type： 杂志文章
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(94)00041-z
更新日期：1995-05-01 00:00:00
abstract：:The hypotheses were: HISS-dependent insulin resistance (HDIR) accounts for insulin resistance that occurs with aging; HDIR is the initiating metabolic defect that leads progressively to type 2 diabetes and the metabolic syndrome; a synergistic antioxidant cocktail in chow confers protection against HDIR, subsequent sy...
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doi：10.1016/j.exger.2008.04.013
更新日期：2008-08-01 00:00:00
