Abstract:
:In this study, we examined effects of retinol deficiency and three retinoids, all-trans-retinoic acid, 13-cis-retinoic acid, and etretin, the aromatic derivative of retinoic acid on nuclear retinoic acid receptor isoforms alpha, beta, and gamma mRNA in rat adipose tissue. Retinol deficiency caused an almost 50% decrease in isoform beta mRNA levels of adipose tissue, whereas little change occurred in the abundance of alpha and gamma isoforms transcripts in this tissue. Intragastric administration of all-trans-retinoic acid to retinol-deficient rats increased the adipose tissue retinoic acid receptor isoforms beta and gamma mRNA levels sixfold and twofold, respectively, in 4 h as compared to adipose tissue of retinol-deficient rats that were administered cottonseed oil. In contrast to this, 13-cis-retinoic acid and etretin at equimolar doses were not effective in inducing beta or gamma isoforms mRNA in retinol-deficient rats. These results show that adipose tissue isoform beta responds to retinol deficiency, and all-trans-retinoic acid rapidly induces beta and gamma mRNA isoforms in this tissue. Thus, retinoic acid may regulate expression of specific genes through its interaction with retinoic acid receptors in adipocytes.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Riaz-ul-Haq,Chytil Fkeywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
381-4issue
3eissn
0022-2275issn
1539-7262journal_volume
33pub_type
杂志文章abstract::The extent to which the heart can convert alpha-linolenic acid (alpha-LNA, 18:3n-3) to longer chain n-3 PUFAs is not known. Conversion rates can be measured in vivo using radiolabeled alpha-LNA and a kinetic fatty acid model. [1-(14)C]alpha-LNA was infused intravenously for 5 min in unanesthetized rats that had been f...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800093-JLR200
更新日期:2008-08-01 00:00:00
abstract::As 5-lipoxygenase (5-LO) is an emerging target in obesity and insulin resistance, we have investigated whether this arachidonate pathway is also implicated in the progression of obesity-related fatty liver disease. Our results show that 5-LO activity and 5-LO-derived product levels are significantly elevated in the li...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800101-JLR200
更新日期:2008-12-01 00:00:00
abstract::Endothelial dysfunction is a key step in atherosclerosis development. Our recent studies suggested that oxLDL-induced increase in endothelial stiffness plays a major role in dyslipidemia-induced endothelial dysfunction. In this study, we identify oxysterols, as the major component of oxLDL, responsible for the increas...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M027102
更新日期:2012-07-01 00:00:00
abstract::In vivo single-voxel magnetic resonance spectroscopy (MRS) at 4.7T and ex vivo high-resolution proton magnetic resonance spectroscopy (HR-NMR) at 500 MHz were used to study the composition of adipose tissues in Zucker obese and Zucker lean rats. Lipid composition was characterized by unsaturation and polyunsaturation ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M011825
更新日期:2011-02-01 00:00:00
abstract::N-3 polyunsaturated fatty acids from marine oil have been shown to decrease T cell-mediated immune function both in animals and humans, and to inhibit the mitogen-induced lymphoproliferative response when added to lymphocyte culture medium. As phosphatidic acid (PA) is a key mediator of the mitogenic process, the pres...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::Diet1 modulates intestinal production of the hormone, fibroblast growth factor (FGF)15, which signals in liver to regulate bile acid synthesis. C57BL/6ByJ mice with a spontaneous Diet1-null mutation are resistant to hypercholesterolemia compared with wild-type C57BL/6J mice through enhanced cholesterol conversion to b...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M078279
更新日期:2018-03-01 00:00:00
abstract::Cerebrosides containing either threo- or erythro-[3-3-H]sphingosine were synthesized by a new procedure. Glucopyranosyl or galactopyranosyl ceramides were converted to their 3-keto derivatives with 2,3-dichloro-5,6-dicyanobenzoquinone and reduced with 3-H-labeled sodium borohydride. The resulting tritiated cerebroside...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract::Normal human plasma HDL was applied to a column of heparin-Sepharose in the presence of MnCl(2) and three fractions were obtained by stepwise elution with increasing NaCl concentrations: a non-retained fraction (NR, 78% of protein) and two retained fractions (R(1) and R(2), 18 and 2.5% of protein, respectively). Both ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::Very few studies have investigated the interrelations between proprotein convertase subtilisin/kexin type 9 (PCSK9) metabolism, cholesterol synthesis, and cholesterol absorption. We aimed to address this issue in a large clinical trial of 245 patients with hypercholesterolemia. Serum lipids, PCSK9, lathosterol (choles...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.P088583
更新日期:2019-01-01 00:00:00
abstract::SREBP cleavage-activating protein (Scap) is an endoplasmic reticulum membrane protein required for cleavage and activation of sterol regulatory element-binding proteins (SREBPs), which activate the transcription of genes in sterol and fatty acid biosynthesis. Liver-specific loss of Scap is well tolerated; hepatic synt...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M059709
更新日期:2015-08-01 00:00:00
abstract::In an effort tao simulate the effects of insulin on fat cells prepared from fresh adipose tissue, pieces of epididymal adipose tissue were cultured in a medium containing charcoal-treated bovine serum albumin (BSA) with a gas phase of 100% O2. Using this system, the insulin effect on [1-14C]glucose oxidation was retai...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::The activity of rat liver microsomal squalene epoxidase is inhibited effectively by digitonin. Concentrations of 0.8 to 1.2 mg/ml of digitonin cause total inhibition of microsomal (0.75 mg protein/ml) squalene epoxidase either in microsomes that were pretreated with digitonin and subsequently washed and subjected to e...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract::A novel and rapid method for the detergent-mediated synthesis of protein-lipid complexes has been developed and has several advantages over detergent dialysis methods. This new method involves co-incubation of human apolipoprotein A-I (apoA-I), the major protein component of high density lipoproteins (HDL), and dipalm...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract::Hypertriglyceridemia results from accumulation of triglyceride (TG)-rich lipoproteins (TRLs) in the circulation and is associated with increased CVD risk. ApoC-III is an apolipoprotein on TRLs and a prominent negative regulator of TG catabolism. We recently established that in vivo apoC-III predominantly inhibits LDL ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M093740
更新日期:2019-08-01 00:00:00
abstract::We have studied the recognition and uptake of acid lipase by human fibroblasts in order to determine requirements for localization and function of the enzyme in lysosomes. Our approach was based on evidence that a number of acid hydrolases involved in mucopolysaccharide metabolism are secreted from cultured fibroblast...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Membranes of mammalian cells contain lysophosphatidic acid acyltransferase (LPAAT) activities that catalyze the acylation of sn-1-acyl lysophosphatidic acid (lysoPA) to form phosphatidic acid. As the biological roles and biochemical properties of the six known LPAAT isoforms have yet to be fully elucidated, we have ch...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M500435-JLR200
更新日期:2006-03-01 00:00:00
abstract::An in vitro method is presented which measures valid, unidirectional uptake rates for lipids across the intestinal brush border. This method combines analysis by a newly devised, double isotope counting system for solubilized tissue with the use of a nonabsorbable marker to correct gross uptake determinations for cont...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1972-03-01 00:00:00
abstract::LY295427, (3 alpha,4 alpha,5 alpha)-4-(2-propenylcholestan-3-ol), acts through an unknown mechanism to derepress the transcription of the low density lipoprotein (LDL) receptor in the presence of 25-hydroxycholesterol (25-OH chol). Preincubation with LY295427 in Chinese hamster ovary (CHO) cells increased uptake of 25...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::Imidazole catalyzed acylations of lysolipids by acyl-CoAs in water at room temperature and at a pH close to neutrality. In the presence of oleoyl-CoA and either lysophosphatidylcholine, 1-palmitoyl-sn-glycero-3-phosphocholine (LPC); lysophosphatidylglycerol, monoacyl-sn-glycero-3-phosphoglycerol; lysophosphatidyl acid...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.m200075-jlr200
更新日期:2002-07-01 00:00:00
abstract::Structurally specific very low density lipoprotein (VLDL) particles are presumed to redirect VLDL uptake during yolk deposition. Egg production is an energy intensive process, but energy excess negatively affects egg production. This study sought to determine whether overfeeding changed hen lipoprotein profiles, and i...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::Fatty acids (FA) that are utilized for triglyceride (TG) synthesis in the liver and principally from two sources: FA synthesized de novo in the liver and preformed FA. We have measured the contribution from the two sources to very low density lipoprotein (VLDL) TG synthesis individually for palmitate, oleate, stearate...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::The secondary bile acid lithocholic acid (LCA) and its derivatives act as selective modulators of the vitamin D receptor (VDR), although their structures fundamentally differ from that of the natural hormone 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3)]. Here, we have determined the crystal structures of the ligand-binding...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M038307
更新日期:2013-08-01 00:00:00
abstract::Small dense LDL (sdLDL) has been reported to be more atherogenic than large buoyant LDL (lbLDL). We examined the metabolism and protein composition of sdLDL and lbLDL in six subjects with combined hyperlipidemia on placebo and rosuvastatin 40 mg/day. ApoB-100 kinetics in triglyceride-rich lipoproteins (TRLs), lbLDL (d...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M073882
更新日期:2017-07-01 00:00:00
abstract::Ac-hE18A-NH2 is a dual-domain apoE mimetic peptide that possesses the putative receptor binding domain from apoE (LRKLRKRLLR, denoted hE; residues 141-150) covalently attached to lipid-associating peptide 18A. Like apoE, Ac-hE18A-NH2 reduces plasma cholesterol in animal models and exhibits anti-inflammatory properties...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M085985
更新日期:2018-11-01 00:00:00
abstract::In mammals, the apolipoprotein (apo) A-I gene is expressed predominantly in liver and intestine, while in avian species it is expressed in all tissues. Although liver and intestine are the major sites of chicken apoA-I mRNA synthesis, there are appreciable amounts of apoA-I mRNA in kidney, ovary/testes, brain, lung, s...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Absorption rates for many biologically important compounds are determined by the relative hydrophobicity of the jejunal microvillus membrane. An estimate of this parameter may be obtained by measuring the incremental change in free energy that occurs when a methylene group partitions into the bilayer form an external ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract::The transfer or exchange of cholesteryl esters, triglycerides, and phospholipids between plasma very low (VLDL), low (LDL), and high (HDL) density lipoproteins is facilitated by specific lipid transfer proteins. The present report describes a method to separate donor and acceptor lipoprotein pools used in assays for l...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1982-05-01 00:00:00
abstract::Mitochondrial cholesterol is maintained within a narrow range to regulate steroid and oxysterol synthesis and to ensure mitochondrial function. Mitochondria acquire cholesterol through several pathways from different cellular pools. Here we have characterized mitochondrial import of endosomal cholesterol using Chinese...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M029942
更新日期:2012-12-01 00:00:00
abstract::Cholesteryl ester storage disease (CESD) and Wolman disease (WD) are both autosomal recessive disorders associated with reduced activity and genetic defects of lysosomal acid lipase (LAL). The strikingly more severe course of WD is caused by genetic defects of LAL that leave no residual enzymatic activity. Mutations a...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::Common forms of metabolic and cardiovascular diseases involve the interplay of numerous genes as well as important environmental factors. Traditional biochemical and genetic approaches generally attempt to dissect these diseases one gene at a time, for example, by analysis of Mendelian forms or genetically engineered ...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R800067-JLR200
更新日期:2009-04-01 00:00:00