Abstract:
:Mitochondrial cholesterol is maintained within a narrow range to regulate steroid and oxysterol synthesis and to ensure mitochondrial function. Mitochondria acquire cholesterol through several pathways from different cellular pools. Here we have characterized mitochondrial import of endosomal cholesterol using Chinese hamster ovary cells expressing a CYP11A1 fusion protein that converts cholesterol to pregnenolone at the mitochondrial inner membrane. RNA interference-mediated depletion of the voltage-dependent anion channel 1 in the mitochondrial outer membrane or of Niemann-Pick Type C2 (NPC2) in the endosome lumen decreased arrival of cholesterol at the mitochondrial inner membrane. Expression of NPC2 mutants unable to transfer cholesterol to NPC1 still restored mitochondrial cholesterol import in NPC2-depleted cells. Transport assays in semi-permeabilized cells showed nonvesicular cholesterol trafficking directly from endosomes to mitochondria that did not require cytosolic transport proteins but that was reduced in the absence of NPC2. Our findings indicate that NPC2 delivers cholesterol to the perimeter membrane of late endosomes, where it becomes available for transport to mitochondria without requiring NPC1.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Kennedy BE,Charman M,Karten Bdoi
10.1194/jlr.M029942subject
Has Abstractpub_date
2012-12-01 00:00:00pages
2632-42issue
12eissn
0022-2275issn
1539-7262pii
jlr.M029942journal_volume
53pub_type
杂志文章abstract::The characteristics of 7 alpha-dehydroxylase, a bile acid-biotransforming enzyme, were determined using dialyzed cell extracts of Eubacterium sp. V.P.I. 12708. 7 alpha-Dehydroxylase was induced by cholic acid in this organism. Induction by cholic acid resulted in the differential synthesis of at least five new polypep...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
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journal_title:Journal of lipid research
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doi:10.1194/jlr.M000976
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M005108
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M013292
更新日期:2011-04-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1966-11-01 00:00:00
abstract::High density lipophorin (HDLp is the main lipoprotein found in resting insect hemolymph. It has, in general, two molecules of apolipoproteins: apoLp-I (250 kDa) and apoLp-II (80 kDa) and a variable lipid content which ranges from 35% to 59% (w/w). Diacylglycerols (DG), phospholipids (PL), and hydrocarbons (HC) are the...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M400425-JLR200
更新日期:2005-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::The ionization behavior of cholic acid, deoxycholic acid, and chenodeoxycholic acid in a variety of physiologically important molecular environments was studied using 13C NMR spectroscopy. The apparent pKa of the carboxyl group was determined from titration curves obtained from the dependence of the carboxyl carbon ch...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章,随机对照试验
doi:10.1194/jlr.P093013
更新日期:2019-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800145-JLR200
更新日期:2008-09-01 00:00:00
abstract::Cigarette smokers have reduced levels of plasma high density lipoprotein (HDL) compared to nonsmokers and are at risk of premature cardiovascular disease. Previous work from this laboratory has shown that exposure of human plasma to gas-phase cigarette smoke (CS) inhibited the activity of lecithin:cholesterol acyltran...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract::Human apolipoprotein C-I (apoC-I) is an exchangeable apolipoprotein that binds to lipoprotein particles in vivo. In this study, we employed a LC-MS/MS assay to demonstrate that residues 38-51 of apoC-I are significantly protected from proteolysis in the presence of 1,2-dimyristoyl-3-sn-glycero-phosphocholine (DMPC). T...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800529-JLR200
更新日期:2009-07-01 00:00:00
abstract::Optically active 1- or 3-acyl-sn-glycerols were synthesized from 2,3- or 1,2-isopropylidene-sn-glycerols, respectively. The 2,3- or 1,2-isopropylidene-sn-glycerols were condensed with appropriate long saturated or unsaturated fatty acids and the resulting acyl isopropylidene compounds were treated with dimethylboronbr...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1997-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.m200163-jlr200
更新日期:2002-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R001891
更新日期:2010-04-01 00:00:00